• Journal of the Korean Society of Food Science and Nutrition
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• v.45 no.1
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• pp.27-34
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• 2016

Garlic has drawn attention as a food material for its anti-oxidative and anti-inflammatory properties as well as for prevention and treatment of cancer. In order to increase efficiency, various aging methods for garlic have been attempted. In particular, thermally processed garlic is known to have higher biological activities due to its various chemical changes during heat treatment. Therefore, in this study, we investigated the anti-oxidative effects of garlic extracts aged at low temperature ($60{\sim}70^{\circ}C$). In the results, 2,2-diphenyl-1-picrylhydrazyl and 2,2-azino-bis (3-ethylbenzo-thiazoline-6-sulfonate) radical scavenging activities and ferric reducing ability of low temperature-aged garlic (LTAG) were similar to those of raw garlic. LTAG also showed decreased lipopolysaccharide (LPS)-induced production of reactive oxygen species, although there were not significant differences among samples. In addition, xanthine oxidase activity was inhibited by LTAG; the 15 days and $60^{\circ}C$ extract showed outstanding inhibition compared with the others. To understand the molecular mechanisms behind the anti-oxidative activity of LTAG, we performed quantitative real-time PCR analysis. The 30 days and $70^{\circ}C$ extract upregulated mRNA expression of antioxidant enzymes such as Cu/Zn-superoxide dismutase (SOD), Mn-SOD, glutathione peroxidase, and catalase in LPS-stimulated RAW 264.7 cells. This result indicates that LTAG can be a functional food as a nature antioxidant and antioxidant substance.

• Journal of Bio-Environment Control
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• v.25 no.1
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• pp.9-15
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• 2016

High temperature is one of the important environmental factors limiting cultivation of apple (Malus domestica Borkh). The expression of genes related with anthocyanin synthesis and sugar accumulation in response to high temperature was studied in the 'Hongro' apple fruits at different developmental stages in different temperature conditions through real-time PCR. Expression of ${\hat{a}}$-amylase (BMY) and polygalacturonase (PG) genes related with sugar synthesis was higher in late ripening stages than in initial ripening stages. Expression of four genes such as phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), and malate dehydrogenase (MDH), which were related with fruit skin coloration, increased gradually in apple fruits of the middle and late ripening stages. Interestingly, the expressions of all genes were highly inhibited expressed at $30-35^{\circ}C$ compared to $25^{\circ}C$ in all ripening stages. In the further work, investigation of expression levels of various genes could be conducted in the level of transcriptomics in fruits at the middle ripening stages to get meaningful information of ripening metabolism in apple in high temperatures.

• Journal of fish pathology
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• v.27 no.1
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• pp.57-65
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• 2014

Copper ($CuSO_4$) has been widely used to control algae and pathogens in fish culture ponds. However, its toxic effects on fish depend not only on its concentration in water but also on water quality. The susceptibility of the rockfish, Sebastes schlegeli to copper was evaluated at three water-temperatures (WT; 18, 23 and $28^{\circ}C$) for 4 days. After the exposure of two copper concentrations (100 and $200{\mu}g/L$), a hematological effect was exerted on rockfish, by causing changes in red blood cell count and hematocrit value at $28^{\circ}C$. Total protein levels of the fish showed a tendency of co-increase with glucose depend on the WT, after copper exposure. However, the plasma calcium and magnesium levels were significantly increased at $200{\mu}g/L$ copper, regardless of the WT. Enzymes activities including ALT and LDH in serum were also significantly increased depend upon the copper treatment only. This indicates that inorganic components and enzymes activities were sensitive indexes to stress by toxicant such as copper. The cortisol levels were significantly elevated by both WT rising and copper treatment in serum of rock fish. In conclusion, these changes can be seen as an initial response to temperature stress and as a sustaining response to copper exposure. The present findings suggest that a simultaneous stress by temperature change and copper exposure could accelerate an alteration of hematological and plasma biological parameters in the rockfish.

• Elastomers and Composites
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• v.47 no.1
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• pp.9-17
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• 2012

Herein, life time prediction based on the deterioration of physical properties of chloroprene rubber (CR)aged by heat and seawater was performed. CR samples were experienced an accelerated test at $80^{\circ}C$, $100^{\circ}C$, $120^{\circ}C$ for heat aging, and $40^{\circ}C$, $60^{\circ}C$, $80^{\circ}C$ for seawater aging for 20,000 hrs. The change in tensile strength, maximum elongation,hardness was measured. As a result, the decrease in elongation was a major factor causing failure. The life time estimated using an Arrhenius model was 125 years at $23^{\circ}C$ for thermal aging and 9 years at $23^{\circ}C$ for seawater aging. SEM and elemental analysis reveal that cracks were generated and the content of oxygen was increased for CR agined by seawater. FT-IR spectrum shows the new C-O and C = O bonds were generated by the chemical reaction with seawater. Also, the glass transtion temperature was increased and the thermal decomposition was decreased by seawater aging.

• Korean Journal of Medicinal Crop Science
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• v.9 no.1
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• pp.1-7
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• 2001

This experiment was carried out to investigate the effects of shading treatments and harvesting methods on the growth of Eleutherococcus senticosus, which was known as the medicine of anticancer, anti-stress, hepatic detoxification activity, immunoactivity, and tonic. Light transmission ratio and air temperature were decreased as $40{\sim}64%$,$1.9{\sim}2^{\circ}C$, respectively, in 30% to 70% shading net treatment compared to non-shading. Fifty percentage shading net treatment was effective for the growth and yield of Eleutherococcus senticosus. During summer Eleutherococcus senticosus was growing under shading net treatment compared to non-shading. Tunnel type was more effective for growth than vertical type in shading method. 30cm length included 2 to 3 nodes cutting from soil surface was the most effective for branching stem length, plant height and yield as harvesting methods.

• Microbiology and Biotechnology Letters
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• v.32 no.4
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• pp.297-306
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• 2004

Heat shock proteins (HSPs) were induced in cells in the thermal stress, and the HSP90 family is one of the major classes of HSPs. Gene encoding HSPs have been characterized from various mammals and piscine. We have cloned and sequenced the HSP90 cDNA from a brain cDNA library constructed from flounder (Paralichthys oliThe result of sequence analysis shows it to be the HSP90~. The nucleotide sequence of the HSP90$\beta$ was composed of 2791 long, encoding 726 amino acid residues. The flounder hsp90$\beta$ gene showed very high sequence homology with hsp90f3 of European sea bass (96.6%), zebrafish (92.9%), Atlantic salmon (92.0%) and human (89.5%). We also constructed a phylogenetic tree based on HSP90 amino acid sequences from vertebrate species. Gene-specific primers were selected and used in RT-PCR reactions to measure the basal hsp90$\beta$ mRNA. The hsp90f3 gene is constitutively expressed at a fairly high level in all examined tissues (brain, liver, kidney, muscle, and spleen). In order to express protein of flounder hsp90$\beta$ in E. coli, we used the His-tagged pETvector. Then, the expression of flounder HSP90$\beta$ was confirmed by Western blot analysis.

• Asian Journal of Turfgrass Science
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• v.17 no.1
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• pp.19-33
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• 2003

The objective of this study was to evaluate the effects of crumb rubber recycled from used tires as a soil incorporation and topdressing materials on a trafficked Korean lawngrass‘Zenith’(Zoysia japonica). In Exp 1, incorporation treatments included three particle sizes (PS: coarse =4∼6.35 mm, medium =2∼4 mm, and One : less than 2 mm in diameter) and two incorporation rate (IR: 10 and 20%). Wear treatments were applied 30 passes per day by compactor weights being 60 kg with soccer shoes. Topdressing treatments included three PS and two topdressing depth (TD: 5 and 10 mm). Wear treatments were the same as described in Exp 1. In Exp 1, the treatment with medium PS+IR 20 resulted in the tendency to have high total clipping yield. There was no significant difference in clipping yield, turfgrass visual color, coverage, and root length among the treatments. Compared to control, tissue Zn levels increased about 6.5-fold by the treatments. The treatment with fine PS +IR 20 caused a less peak deceleration than coarse PS +IR 10. Total porosity, air-filled porosity, and capillary porosity increased with fine PS +IR 20. In Exp 2, compared to controls, however, there was a difference in turfgrass visual color after the termination of traffic treatment. There was no difference in root length. The treatment with fine PS + TD 10 resulted in the highest total clipping yield. As a result of soil physical analysis, soil penetration resistance was reduced by the treatments. The treatment with coarse PS resulted in a less peak deceleration than fine PS. In conclusion, turfgrass growth was increased by crumb rubber incorporation which enhanced soil physical properties. The crumb rubber topdressing was able to cushion the crown tissue area while still providing a smooth and uniform surface, improve overall turfgrass quality, and reduce compaction.

• Asian Journal of Turfgrass Science
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• v.15 no.4
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• pp.169-179
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• 2001

This study was conducted to determine the effect of root zone cooling on growth and quality of turfgrasses including Kentucky bluegrass (Poa pratensis L.‘Nuglade’), perennial ryegrass (Lolium perenne L.‘Accent’), tall fescue (Festuca arundinacea Schreb.‘Pixie’), and Japanese lawngrass (Zoysia japonica Steud.) in simulated athletic field during summer season in Korea. Mineral contents in clippings of turfgrasses grown at different soil mixtures and temperatures were also analyzed. Root zone cooling (approximately 4~6$^{\circ}C$ lower than that of untreated-control) resulted in good uniformity, little disease incidence and higher level of chlorophyll contents in cool-season turfgrasses. The effectiveness of root zone cooling in protecting disease incidence from high temperature stress was the most manifest in perennial ryegrass compared to others. Fresh clipping weight in treatment of root zone cooling was increased approximately 2 times in Kentucky bluegrass and perennial ryegrass, and 2.5 times in tall fescue compared to those of control. There was higher growth rate in a soil mixture composed of 80% peat moss ＋10% sand ＋10% soil (v/v/v) than in that of 80% pea moss ＋20% sand (v/v), Mineral contents of N, P, K, Ca, and Mg in clippings of three species of cool-season turfgrasses were significantly increased in treat-ment of root zone cooling but this was not found in Japanese lawngrass. Results showed that root zone cooling has a benefit in keeping good quality and growth of cool-season turfgrasses in sports field under supraoptimal ambient temperature during summer season.

• Journal of Life Science
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• v.17 no.12
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• pp.1649-1653
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• 2007

High temperature stresses have caused growth inhibition and delayed heading in highland cultivation Chinese cabbage during summer in Korea. We have studied high temperature stress responses in the terms of changes of inorganic components and proteins by proteomic analyses. Insufficiencies of nitrogen and phosphorus have affected growth rate and calcium deficiency has caused blunted heading. Proteins extracted from Brassica seedling grown at the altitude of 600m and 900m in the Mount Jilun were extracted and analysed by 2-dimentional polyacrylamide gel electrophoresis. Profiles of protein expression was then analyzed by 2-dimentional gel analyses. Protein spots showing different expression level were picked using the spot handling workstation and subjected to MALDI-TOF MS. Total 48 protein spots were analyzed by MALDI-TOF MS and 30 proteins spots out of 48 were identified by peptide mass fingerprinting analyses. Fourteen proteins were up-regulated in extracts from the altitude of 900m and they were identified as oxygen-evolving proteins, rubisco activase and ATPase etc. Sixteen proteins were up-regulated in extracts from the altitude of 600m and they were identified as glutathione S-transferase(1, 28kD cold induced- and 24 kD auxin-binding proteins) and salt-stress induced protein etc. These stress-induced proteins were related to the mediated protective mechanism against oxidative damage during various stresses. The results indicated that physiological phenomenon in response to high temperature stresses might be resulted by complex and multiple array of responses with drought, heat, oxidative, salt, and cold by high temperature.

• Resources Recycling
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• v.29 no.1
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• pp.25-34
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• 2020

Electrochemical Quartz Crystal microbalance is a tool that is capable of measuring nanogram-scale mass change on electrode surface. When applying alternating voltage to the quartz crystal with metal electrode formed on both sides, a resonant frequency by inverse piezoelectric effect depends on its thickness. The resonant frequency changes sensitively by mass change on its electrode surface; frequency increase with metal dissolution and decrease with metal deposition on the electrode surface. The relationship between resonant frequency and mass change is shown by Sauerbrey equation so that the mass change during metal dissolution can be measured in real time. Especially, it is effective in the case of reaction mechanism and rate studies accompanied by precipitation, volatilization, compound formation, etc. resulting in difficulties on ex-situ AA or ICP analysis. However, it should be carefully considered during EQCM experiments that temperature, viscosity, and hydraulic pressure of solution, and stress and surface roughness can affect on the resonant frequency. Application of EQCM was shown as a case study on leaching of platinum using aqueous chlorine for obtaining activation energy. A platinum electrode of quartz crystal oscillator with 1000 Å thickness exposed to solution was used as leaching sample. Electrogenerated chlorine as oxidant was purged and its concentration was controlled in hydrochloric acid solution. From the experimental results, platinum dissolution by chlorine is chemical reaction control with activation energy of 83.5 kJ/mol.