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http://dx.doi.org/10.12791/KSBEC.2016.25.1.9

Expression of Genes Affecting Skin Coloration and Sugar Accumulation in 'Hongro' Apple Fruits at Ripening Stages in High Temperatures  

Kim, Seon Ae (Department of Horticulture and Life Science, Yeungnam University)
Ahn, Soon Young (Department of Horticulture and Life Science, Yeungnam University)
Yun, Hae Keun (Department of Horticulture and Life Science, Yeungnam University)
Publication Information
Journal of Bio-Environment Control / v.25, no.1, 2016 , pp. 9-15 More about this Journal
Abstract
High temperature is one of the important environmental factors limiting cultivation of apple (Malus domestica Borkh). The expression of genes related with anthocyanin synthesis and sugar accumulation in response to high temperature was studied in the 'Hongro' apple fruits at different developmental stages in different temperature conditions through real-time PCR. Expression of ${\hat{a}}$-amylase (BMY) and polygalacturonase (PG) genes related with sugar synthesis was higher in late ripening stages than in initial ripening stages. Expression of four genes such as phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), flavanone 3-hydroxylase (F3H), and malate dehydrogenase (MDH), which were related with fruit skin coloration, increased gradually in apple fruits of the middle and late ripening stages. Interestingly, the expressions of all genes were highly inhibited expressed at $30-35^{\circ}C$ compared to $25^{\circ}C$ in all ripening stages. In the further work, investigation of expression levels of various genes could be conducted in the level of transcriptomics in fruits at the middle ripening stages to get meaningful information of ripening metabolism in apple in high temperatures.
Keywords
anthocyanin; high temperature; real-time PCR; ripening metabolism;
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1 KOSAT. 2014. http://kosat.go.kr/potal/korea/kor-nw/2/7/1/index.board.
2 Atkinson, R.G., R. Schroder, I.C. Hallett, D. Cohen, and E.A. MacRae. 2002. Overexpression of polygalacturonase in transgenic apple trees leads to a range of novel phenotypes involving changes in cell adhesion. Plant Physiol. 129:122-133.   DOI
3 Azuma, A., H. Yakushiji, Y. Koshita, and S. Kobayashi. 2012. Flavonoid biosynthesis-related genes in grape skin are differentially regulated by temperature and light conditions. Planta 236:1067-1080.   DOI
4 Ban, Y., S. Kondo, B.E. Ubi, C. Honda, H. Bessho, and T. Moriguchi. 2009. UDP-sugar biosynthetic pathway: contribution to cyaniding 3-galactoside biosynthesis in apple skin. Planta. 230: 871-881.   DOI
5 Barritt, B.H., C.R. Rom, K.R. Guelich, S.R. Drake, and M.A. Dilley. 1991. Light level influences spur quality and canopy development and light interception influence fruit production in apple. Hort Science 26:993-999.
6 Bergqvist, J., N. Dokoozlian, and N. Ebisuda. 2001. Sunlight exposure and temperature effects on berry growth and composition of Cabernet Sauvignon and Grenache in the central San Joaquin valley of California. Am. J. Enol. Vitic. 52:1-7.
7 Curry, E.A. 1997. Temperatures for optimal anthocyanin accumulation in apple tissue. J. Hort. Sci. 72:723-729.
8 Beruter, J. 1985. Sugar accumulation and changes in the activities of related enzymes during development of the apple fruit. J. Plant Physiol. 121:331-341.   DOI
9 Chang, S., J. Puryear, and J. Cairney. 1993. A simple and efficient method for isolating RNA from pine trees. Plant Mol.Biol. 11:113-116.   DOI
10 Costa, F., C.P. Peace, S. Stella, S. Serra, S. Musacchi, M. Bazzani, S. Sansavini, and W.E. Van de Weg. 2002. QTL dynamics for fruit firmness and softening around an ethylene-dependent polygalacturonase gene in apple (Malus x domestica Borkh.). J. Exp. Bot. 1-11.
11 Honda, C., N. Kotoda, M. Wada, S. Kondo, S. Kobayashi, J. Soejima, Z. Zhang, T. Tsuda, and T. Moriguchi. 2002. Anthocyanin biosynthetic genes are coordinately expressed during red coloration in apple skin. Plant Physiol. Biochem. 40: 955-962.   DOI
12 Hulme, M., Z.C. Zaho, and T. Jiang. 1994. Recent and future climate change in East Asia. Intl. J. Climatol. 14:637-658.   DOI
13 Ju, Z., C. Liu, and Y. Yuan. 1995a. Activities of chalcone synthase and UDPGal:flavonoid 3-O-glycosyltransferase in relation to anthocyanin synthesis in apple. Sci. Hortic. 63:175-185.   DOI
14 Ju, Z., C. Liu, Y. Yuan, C. Liu, and S. Xin. 1995b. Relationships among phenylalanine ammonia-lyase activity, simple phenol concentrations and anthocyanin accumulation in apple. Sci. Hortic. 61:215-226.   DOI
15 Kim, S.Y., I.H. Heo, and S.H. Lee. 2010. Impacts of temperature rising on changing of cultivation area of apple in Korea. J. Kor. Assoc. Reg. Geograph.. 16:201-215. (in Korean)
16 Lee, H.C. 1999. Physiologycal and ecological factors affecting fruit coloration and color enhancement in Malus domestica Borkh. cv. 'Fuji'. Ph. D. Thesis. Seoul National University, Korea. (in Korean)
17 Kondo, S., K. Hiraoka, S. Kobayashi, C. Honda, and N. Terahara. 2002. Changes in the expression of anthocyanin biosynthetic genes during apple development, J. Am. Soc. Hort. Sci. 127:971-976.
18 Lakso, A.N. 1994. Apple. Handbook of Environmental Physiology of Fruit Crops. 1:3-42.
19 Lancaster, J.E. 1992. Regulation of skin color in apples. Crit. Rev. Plant Sci. 10:487-502.   DOI
20 Lee, J.C., T. Tomana, U. Naoki, and K. Ikuo. 1979. Physiological study on the anthocyanin development in grape - I. Effect of fruit temperature on the anthocyanin development in "Kyoho" grape. Kor. J. Hort. Sci. Technol. 20:55-65. (in Korean)
21 Lister, C.E., J.E. Walker, and J.R.L. Lancaster. 1996. Developmental changes in enzymes of flavonoid biosynthesis in the skins of red and green apple cultivars. J. Sci. Food Agric. 71:313-320.   DOI
22 Noro, S., H. Ichinohe, and N. Obra. 1991. Effects of controlled air temperature on development of anthocyanin, sugar and citramalic acid during maturation of the apple cultivar Starking Delicious. Bulletin of the Aomori Apple Experiment Station. 27:111-123.
23 Park, M.Y., Y.Y. Song, H.H. Han, and D.H. Sagong. 2009. Influence of air temperature during the growing period on water core occurrence in 'Hongro' apple cultivar and the mitigation technique. Korean J. Agr. Forest Meteorol. 11:100-110. (in Korean)   DOI
24 Song, K.J., J.H. Hwang, and H.K. Yun. 2003. Changes of soluble sugar and starch concentrations in fruits of apple cultivars differing in maturity. Hort. Environ. Biotechnol. 44:207-210.
25 Reay, P.F. 1999. The role of low temperatures in the development of the red blush on apple fruit ('Granny Smith'). Sci. Hortic. 79:113-119.   DOI
26 Sagong, D.H., H.J. Kweon, M.Y. Park, Y.Y. Song, S.H. Ryu, M.J. Kim, K.H. Choi, and T.M. Yoon. 2013. Impacts of urban high temperature events on physiology of apple trees: a case study of 'Fuji'/M.9 apple trees in daegu, korea. Korean J. Agr. Forest Meteorol. 15:130-144. (in Korean)   DOI
27 Saure, M.C. 1990. External control of anthocyanin formation in apple. Sci. Hortic. 42:181-218.   DOI
28 Seo, H.H. and H.S. Park. 2003. Fruit quality of 'Tsugaru' apples influenced by meteorological elements. Korean J. Agr. Forest Meteorol. 54:218-225.
29 Song, J.H., I.K. Kang, and D.G. Choi. 2014. Light conditions and characteristics of leaves and fruit at different canopy positions in slender-spindle 'Hongro' apple trees. Kor. J. Hort. Sci. Technol. 32:440-447.
30 Steyn, W.J., D.M. Horlcroft, S.J.E. Wand, and G. Jacobs. 2004. Anthocyanin degradation in detached pome fruit with reference to preharvest red color loss and pigmentation patterns of blushed and fully red pears. J. Amer. Soc. Hort. Sci. 129:13-19.
31 Steyn, W.J., S.J.E. Wand, D.M. Holcroft, and G. Jacobs. 2005. Red colour development and loss in pears. Acta Hortic. 671:79-85.
32 Tomana, T. and H. Yamada. 1988a. Change in sugar composition during maturation stage of apple fruit grown at different locations. J. Jpn. Soc. Hortic. Sci. 57:178-183.   DOI
33 Yoon, C.G., I.C. Son, and D.I. Kim. 2010. Effects of temperature during growth stage on tree growth and fruit quality in 'Fuji'/M.9 apples. J. Kor. Soc. Int. Agric. 22:143-147.
34 Tomana, T. and H. Yamada. 1988b. Relationship between temperature and fruit quality of apple cultivars grown at different locations. J. Jpn. Soc. Hortic. Sci. 56:391-397.   DOI
35 Ubi, B.E., C. Honda, H. Bessho, S. Kondo, M. Wada, S. Kobayashi, and T. Moriguchi. 2006. Expression analysis of anthocyanin biosynthetic genes in apple skin: Effect of UV-B and temperature. Plant Sci. 170:571-578.   DOI
36 Warrington, I.J., T.A. Fulton, E.A. Halligan, and H.N. de Silva. 1999. Apple fruit growth and maturity are affected by early season temperatures. J. Am. Soc. Hortic. Sci. 124(5):468-477.
37 Yamada, H., H. Ohmura, C. Arai, and M. Terui. 1994. Effect of preharvest fruit temperature on ripening, sugar, and watercore occurrence in apples. J. Am. Soc. Hortic. Sci. 119(6):1208-1214.
38 Yamada, H., K. Hamamoto, A. Sugiura, and T. Tomana. 1988. Effect of controlled fruit temperature on maturation of apple fruits. J. Jpn. Soc. Hortic. Sci. 57(2):173-177.   DOI