• Journal of the Korea Organic Resources Recycling Association
• /
• v.28 no.4
• /
• pp.77-85
• /
• 2020

This study analyzes the effects of mixed fermented organic fertilizer on chinese cabbage growth and soil properties in order to investigate the nutritional effects of organic fertilizers, which are developed as an alternative fertilizer for imported castor oil cake. In this study, four treatments were set up: 100% and 200% rate of nitrogen application (320 kg ha-1 for Chinese cabbage) on mixed fermented organic fertilizer A(FA) and mixed fermented organic fertilizer B(FB), respectively, 100% rates of the mixed expeller cake (MEC) fertilizer, and the untreated control. Results revealed that the growth and yield of Chinese cabbage increased as more fermented organic fertilizer was used. However, while there were no significant differences in growth characteristics between treatments of 100% rate of mixed fermented organic fertilizer and 100% rate of MEC, the impacts on yields resulted similar. The nitrogen use efficiency (NUE) of Chinese cabbage was measured a range of 20-31% depending on the response to treatment. The 100% FA showed the same as NUE and nitrogen absorption with 100% rate of MEC. Regarding soil properties after cultivation, there were no significant differences among the effects of fertilizers in pH, EC, soil organic matter, and available phosphate. However, the content of exchangeable cations(K, Ca, Mg) was higher in areas treated with mixed fermented organic fertilizer than in untreated areas. Furthermore, the bacterial population density in the soil was higher in areas treated with mixed fermented organic fertilizer than in untreated areas and increased as more mixed fermented organic fertilizer was used. There were no significant differences in the population density of actinomycetes and fungi when fertilizer was applied to the soil. These results also show that FA, as a alternative organic fertilizer for imported castor oil cake, has similar nutritional effects as that of MEC. Therefore, further research the appropriate amounts of fertilizer is required to achieve economical and eco-friendly nutrient management.

• Tuberculosis and Respiratory Diseases
• /
• v.44 no.6
• /
• pp.1343-1352
• /
• 1997

Background : Heat-treated cells are known to be protected from lysis by TNF, which is considered to play a central role in the pathogenesis of sepsis-induced acute lung injury. The objective of the study was to investigate the effect of heat shock response by heat-pretreatment on the acute lung injury of the rats induced by intratracheally administered TNF-$\alpha$, Methods : We intratracheally instilled either saline or TNF (R&D, 500ng) with and without heat pretreatment in Sprague-Dawley rats weighing 250~350 g. The heated rats were raised their rectal temperature to $41^{\circ}C$ and was maintained thereafter for 13 minutes at 18 h before intratracheal administration of saline or TNF. After 5 h of intratracheal treatment, lung leak, lung myeloperoxidase activity (MPO) and heat shock proteins were measured in rats. Lung leak index was defined as counts per minute of $I^{25}$ in the right lung divided by counts per minutes of $I^{25}$ in 1.0 ml of blood. All data are expressed as means ${\pm}$SE. Results : There is no difference in acute lung leak index ($0.099{\pm}0.024$ vs $0.123{\pm}0.005$) among the rats given saline intratracheally with and without heat pretreatment, but MPO activity showed a decreased tendency in heat-pretreated rats ($4.58{\pm}0.79\;U/g$) compared with heat-unpretreated rats ($7.32{\pm}0.97\;U/g$) (P=0.064). Rats administered TNF intratracheally with heat-pretreatment had decreased lung leak index ($0.137{\pm}0.012$) and lung MPO activity ($5.51{\pm}1.04\;U/g$) compared with those of heat-unpretreated and TNF-administered rats ($0.186{\pm}0.016$, $14.34{\pm}1.22\;U/g$) (P<0.05 in each). There were no significant difference of lung leak index and MPO activity between TNF-treated rats with heat-pretreatment and saline-treated rats with and without heat-pretreatment. Conclusion : The heat shock response attenuated neutrophil recruitment and acute lung leak induced by intratracheal instillation of TNF-in rats.

• Journal of The Korean Society of Inherited Metabolic disease
• /
• v.16 no.2
• /
• pp.93-101
• /
• 2016

Purpose: McArdle disease, glycogen storage disease type V (GSD V), is one of the most common adolescent-onset glycogen storage diseases. It is caused by recessive mutations in PYGM encoding myophosphorylase, which is critical to glycogen metabolism. Since only a few korean patients have been reported, we will observe the clinical and genetic features of three korean patients with McArdle disease. Methods: We retrospectively reviewed the medical records of three patients with genetically confirmed McArdle disease, including the results of forearm ischemic exercise test, electromyogram, nerve conduction velocity, muscle biopsy, and PYGM analysis in peripheral leukocytes. Results: All three cases were males and their age of symptom onset was 12, 5, 14 years old, respectively. A high basal level of serum creatine kinase was noted in all three patients. They experienced the recurrent episodes of rhabdomyolysis, but second wind phenomenon was not definite. In muscle biopsy, subsarcolemmal space vacuoles including periodic acid schiff stained materials were found in two patients, while no evidence of glycogen storage disease was found in the other. A total of five different mutations, $p.Arg50^*$, p.Trp798Arg, $p.Arg50^*$, p.Glu779del, $p.Asp511Thrfs^*28$ and p.Phe710del, were found in three patients. Avoidance of isometric exercise, aerobic exercise and glucose intake before each exercise were recommended for all patients. Conclusion: The three Korean patients with McArdle disease showed the typical manifestations of the condition. The most mutations were private. Therefore, identification of more cases with long-term follow-up will be required to understand the clinical and genetic features of this disease among Korean population.

• Clinical and Experimental Reproductive Medicine
• /
• v.36 no.4
• /
• pp.311-319
• /
• 2009

Objective: Human embryonic stem cells (hESCs) have the capacity to differentiate into all of the cell types and therefore hold promise for cell therapeutic applications. In order to utilize this important potential of hESCs, enhancement of currently used technologies for handling and manipulating the cells is required. The cryopreservation of hESC colonies was successfully performed using the vitrification and slow freezing-rapid thawing method. However, most of the hESC colonies were showed extremely spontaneous differentiation after freezing and thawing. In this study, we were performed to rapidly collect of early passage hESCs, which was thawed and had high rate of spontaneously differentiation of SNUhES11 cell line. Methods: Four days after plating, partially spontaneously differentiated parts of hESC colony were cut off using finely drawn-out dissecting pipette, which is mechanical separation method. Results: After separating of spontaneously differentiated cells, we observed that removed parts were recovered by undifferentiated cells. Furthermore, mechanical separation method was more efficient for hESCs expansion after thawing when we repeated this method. The recovery rate after removing differentiated parts of hESC colonies were 55.0%, 74.5%, and 71.1% when we have applied this method to three passages. Conclusion: Mechanical separation method is highly effective for rapidly collecting and large volumes of undifferentiated cells after thawing of cryopreserved early passage hESCs.

• Clinical and Experimental Reproductive Medicine
• /
• v.36 no.4
• /
• pp.283-292
• /
• 2009

Objective: Human embryonic stem cells (hESCs) can proliferate indefinitely and differentiate into all kinds of cell types in vitro. Therefore, hESCs can be used as a cell source for cell-based therapy. Transduction of foreign genes to hESCs could be useful for tracing differentiation processes of hESCs and elucidation of gene function. Thus, we tried to introduce enhanced green fluorescent protein (eGFP) gene to hESCs, XX and XY cell lines in this study. Methods: Lentivirus containing eGFP was packaged in 293T cells and applied to hESCs to transduce eGFP. Expression of transduced eGFP was evaluated under the fluorescence microscope and eGFP positive population was analyzed by FACS. Expression of undifferentiation state markers such as Oct4, Nanog, SSEA4 and Tra-1-81 was examined by RT-PCR and/or immunofluorescence in eGFP-hESCs after transduction. In addition, the ability of eGFP-hESCs to form embryoid bodies (EBs) was tested. Results: eGFP was successfully transduced to hESCs by lentivirus. eGFP expression was stably maintained up to more than 40 passages. eGFP-hESCs retained expression patterns of undifferentiation state markers after transduction. Interestingly, disappearance of transduced eGFP was notably observed during spontaneous differentiation of eGFP-hESCs. Conclusion: We established eGFP expressing hESC lines using lentivirus and showed the maintenance of undifferentiation characteristics of these eGFP-hESCs. This reporter-containing hESCs could be useful for tracing the processes of differentiation of hESCs and other studies.

• Food Science and Preservation
• /
• v.9 no.3
• /
• pp.271-276
• /
• 2002

To develop a wrapping film, which suppresses the microbial decay through the storage and prolongs the selflife of fruits and vegetables, the antimicrobial packaging films were prepared and applied to the preservation of strtwberries and cucumbers. Low density polyethylene(LDPE) film of 50㎛ thickness was faricated with 1% of Scutellariae baicalensis extract. The LDPE film impregnated with Scutellariae baicalensis extract showed antimicrobial activity on the disk test against Bacillus cereus, Escherchia coli and Fusarium sp.. The antimicrobial film changed the color and light transmittance, but did not affect heat shrinkage, mechanical tensile strength and wattability. Strawberries and cucumbers were separately wrapped with packaging films in the state of closely-adhered packaging as well as modified atmosphere packaging(MAP). The wrapped strawberries and cucumbers were stored for 21 days at 5$\^{C}$ and for 40 days at l0$\^{C}$, respectively. For the packaged strawberries and cucumbers at 5$\^{C}$ and 10$\^{C}$, the LDPE film impregnated with Scutellariae baicalensis extract showed the reduced growth of total aerobic bacteria, molds and yeasts and did not give any negative effect on other quality attributes during storage in comparison with conttrol film without any additive.

• Economic and Environmental Geology
• /
• v.51 no.5
• /
• pp.401-407
• /
• 2018

For the treatment of heavy metals in the mine drainage from the closed mine area, various methods such as passive, active and semi-active treatments are considered. Among contaminated elements in the mine drainage, Mn is one of the difficult elements for the treatment because it needs high pH over 9.0 for its concentration to be reduced. In this study, the efficiency of various slag complex reactors (slag (S), slag+limestone (SL) and slag+Mn coated gravel (SG)) on Mn removal in the presence of Fe, which is a competitive element with Mn, was evaluated to investigate effective methods for the treatment of Mn in mine drainage. As a result of experiments on Mn removal without Fe during 358 days, using influent with $30{\sim}50Mn{\cdot}mg/L$ and pH 6.7 on the average, S reactor showed continuously high Mn removal efficiency with the average of 99.9% with pH 8.9~11.4. Using the same reactors, Mn removal experiments with Fe during 237 days were conducted with the influent with $40{\sim}60Mn{\cdot}mg/L$. The pH range of effluent reached to 6.1~10.0, which is slightly lower than that of effluent without Fe. S reactor showed the highest range of pH with 7.1~9.9, followed by S+L and S+G reactor. However, the efficiency of Mn removal showed S+L>S>S+G with the range of 94~100%, 68~100% and 68~100%, respectively in spite of relatively low pH range. S+L reactor showed the most resistance on Fe input, which means other mechanisms such as $MnCO_3$ formation by the carbonate prouced from the limestone or autocatalysis reaction of Mn contributed to Mn removal rather than pH related mechanisms. The evidence of reactions between carbonates and Mn, rhodochrosite ($MnCO_3$), was found from the X-ray diffraction analysis of precipitates sample from S+L reactor. From this study, the most effective reactors on Mn removal in the presence of Fe was S+L reactor. The results are expected to be applied for the Mn containing mine water treatment in the presence of Fe within the relatively low range of pH.

• Proceedings of the Korean DIstribution Association Conference
• /
• 2001.11b
• /
• pp.125-141
• /
• 2001

In this paper, the current problems of logistics industry in Korea and their possible solutions were discussed. With Korea Telecoms KT-Logis, the supplier and demander of logistics service would not have to invest large sum of money into their computer system. All they need is just a computer with internet connected. What KT-Logis influence on the logistics industry are the following; 1. Many logistics service supplier and demander can do the business on the web with one computer system. 2. This web based computer system does not only work on the office but also apply on the field worker such as delivery personnel or even the forwarder with mobile phone. 3. KT-Logis is an integrated system which cover the broad arrange of logistics management from truck management to customer relations management. 4. Finally, KT-Logis is web based systems which suits for current e-business and mobile environment. In future, more studies should be done to develop more progressive integrated logistics information systems with enterprise resource planning(ERP) and supply chain management(SCM).

• Journal of the Korean earth science society
• /
• v.22 no.5
• /
• pp.377-387
• /
• 2001

In order to elucidate sedimentation processes and depositional environments in transitional area between continental shelf and coastal zone, sedimentologic study has been done with 84 surface sediments sampled in nearshore/offshore off the mid-western coast of the Korean Peninsula for 3 years (1996${\sim}$1999). The surface sediment can be classified into 4 facies (gravelly sand, sand, silty sand and sandy silt). Mean grain size, sorting, skewenss and kurtosis varies -0.39${\sim}7.82{\Phi}$, 0.36${\sim}4.68{\Phi}$, -0.38${\sim}$0.86, -1.56${\sim}$3.43, respectively. The textural parameters show a finer-grained and poorly-sorted trend shoreward, northward and southward from the central part of the study area. The positively-skewed distribution and relationship of each textural parameters indicate a tide-dominated depositional environment. According to C/M diagram, there are 3 different domains (mode A, B, C) of sediment transport mode. The northern part is characterized by bedload transport (mode A) and represents co-influence of wave and tide, whereas domain C in the southern part is controlled by uniform suspension transport (mode C), correlating with sandy-silt area. In the broad middle area, transport processes are complex (the mixture of bedload, graded suspension and uniform suspension; mode B). Hence, the subdivision depositional environments of this study area may be classified by 3 depositional environments dependent on the interplay of sediment supplies from river, relict sediments and hydrologic conditions. In results, the nearshore and offshore areas are thus characterized as a mixing zone between coastal terrigenous sediments and relict sediments in the continental shelf by complex processes (tide, wave and river flow). These sedimentation processes play an important role in producing distinct sedimentologic features in the transitional zone linking coastal and shelfal areas.

• Reproductive and Developmental Biology
• /
• v.36 no.3
• /
• pp.147-154
• /
• 2012

This study was designed to determine whether low-density lipoproteins (LDL) from egg yolk and taurine, hypotaurine and trehalose as antioxidant in extender improve the freezability and fertility of Korean Jeju Black Bull semen. The semen was cryopreserved with tris egg yolk extenders containing 7% glycerol and treated 4% LDL, 20 mM taurine, hypotaurine and trehalose. Frozen-thawed sperm were evaluated motility, viability, membrane, and acrosome integrity and sperm penetration ability. The results were compared to semen cryopreserved in tris egg yolk extender only as control. Frozen-thawed semen evaluation cleary indicated that the addition of LDL and LDL-antioxidants (taurine, hypotaurine and trehalose) combination were significantly improved (p<0.05) the viability (%; with staining test using eosin-Y) compared to control spermatozoa. Also, in membrane integrity (%; with supravital hypo-osmotic swelling test), not only LDL-antioxiants combination but also LDL were significantly increased (p<0.05) the swelled sperm using HOST compared to control. Sperm acrosome integrity state was classified by CTC (chlortetracycline) staining test. F pattern was significantly increased in LDL-antioxidant combination than control (p<0.05) and B pattern was not significantly differences among all treatments and control. However, AR pattern was significantly decreased in LDL-antioxidants combination than control (p<0.05). Pronucleus formation and sperm penetration index (SFI) were significantly increased in LDL and LDL-antioxidants combination than control (p<0.05). Especially, LDL-taurine significantly improved pronucleus fomation and SFI than LDL (p<0.05). It was concluded that LDL and LDL-antioxidants in extender improved the freezability and fertility of Korean Jeju Black bull spermatozoa.