• Title/Summary/Keyword: 오약순기산

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Therapeutic Effect of Oyaksungisan in Dogs with Facial Nerve Paralysis (개 안면신경마비에 대한 오약순기산의 치료효과)

  • Eom, Chang-Sub;Jun, Hyung-Kyou;Kim, Sang-Hun;Yun, Hyo-In;Kim, Myung-Cheol;Kim, Duck-Hwan
    • Journal of Veterinary Clinics
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    • v.25 no.4
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    • pp.245-248
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    • 2008
  • Herbal medicines are widely used to treat a variety of human diseases. However, therapeutic effect of herbal medicine on the canine facial nerve paralysis (FNP) has not been investigated. This study examined therapeutic effects of Oyaksungisan on the canine FNP. Ten dogs with the induced canine FNP were equally divided into the control and an herbal medicine-treated groups (5 dogs, Oyaksungisan-treated group), respectively. No treatment was given to the control group. In the Oyaksungisan-treated group, Oyaksungisan was administered orally, twice per day for 2 weeks (50 mg/kg) after inducing FNP. Significant differences in the clinical scores were detected between the Oyaksungisan-treated and control groups on day 14 (p < 0.05). The serum creatine kinase (CK) activities in the Oyaksungisan-treated group showed a tendency to decrease but there was no significant difference compared with those of the control group. In conclusion, Oyaksungisan was effective for the induced canine FNP.

Effects of Oyaksoonkisan and Kamioyaksoonkisan on Hypertension and Pulse Rate (오약순기산과 가미오약순기산이 고혈압과 심박동수에 미치는 영향)

  • Jun, Sung-Bae;Kim, Byung-Tak;Lim, Rak-Chul;Kim, Sung-Hoon
    • The Journal of Korean Medicine
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    • v.18 no.1
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    • pp.267-277
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    • 1997
  • Experiments were performed to determine the effects of the Oyacksunkisan(烏藥順氣散) and Kamioyacksunkisan(加味烏藥順氣散) liquid extract on the hypertension and the pulse rate in Sprague-Dawley rat(SDR) and Spontaneous Hypertensive rats(SHR). The results were obtained as follows ; 1. Blood pressure was significantly decreased firstday and 11th day after administration of Oyacksunkisan extract and pretreatment of phentolamine. 2. Blood pressure and pulse rate were regulated first day after administration of Kamioyacksunkisan and blood pressure was significantly decreased after pretreatment of clonidine and phentolamine. 3. Oyacksunkisan and Kamioyacksunkisan didn't show any significant changes of blood pressure and pulse rate after pretreatment of prdpranolol, hydralazine and verapamil From the above result, it was concluded that Oyacksunkisan(烏藥順氣散) and Kamioyacksunkisan(加味烏藥順氣散) could be applied effectively to the hypertension.

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Effects of OYakSoonGi-San extract on Hypertension and Common Carotid Artery (오약순기산(烏藥順氣散)이 고혈압과 동맥혈관에 미치는 영향)

  • Shin, Hyung-Sup;Ko, Heung;Kim, Ho-Hyun
    • Korean Journal of Oriental Medicine
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    • v.10 no.2
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    • pp.79-92
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    • 2004
  • Objectives : This experiments were performed to determine the effect of OYakSoonGi-San extract on hypertension in spontaneous hypertensive rat and norepinephrine-induced arterial contraction in rabbit. Methods : In order to define the effect of OYakSoonGi-San extract on contracted rabbit carotid arterial strips, transverse strips with intact or damaged endothelium were used for the experiment using organ bath. To analyze the mechanism of OYakSoonGi-San extract-induced relaxation, OYakSoonGi-San extract infused into contracted arterial strips induced by norepinephrine after treatment of indomethacin, $N{\omega}-nitro-L-arginine$, methylene blue or tetraethylammonium chloride. Results : Blood pressure was significantly decreased five days after administration of OYakSoonGi-San extract. The relaxation effect of OYakSoonGi-San extract was dependent on the presence of endothelium, showing that OYakSoonGi-San extract-induced relaxation was not observed in the strips without endothelium. Also OYakSoonGi-San extract-induced relaxation was significantly inhibited in arterial strips which were contracted by high $K^+$. OYakSoonGi-San extract-indeced relaxation was significantly inhibited by the pre-treatment of $N{\omega}-nitro-L-arginine$ or methylene blue, but it was not observed in the strips pre-treated with indomethacin or tetraethylammonium chloride. When additive application of $Ca^{2+}$ in arterial strips which were pre-contracted by norepinephrine in a $Ca^{2+}$-free solution, arterial contraction was increased. But contractile response to $Ca^{2+}$ was attenuated by pre-treatment of OYakSoonGi-San extract. Conclusions : These results demonstrated that OYakSoonGi-San could be applied effectively to hypertension and may inhibit agonist-induced contraction through an decrease influx of extra-cellular $Ca^{2+}$ by the formation of nitric oxide in the vascular endothelial cells.

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Studies on the Anti-inflammatory and Analgesic Activities of Ohyaksungisan (오약순기산(烏藥順氣散)의 소염 진통작용에 관한 연구)

  • Lee, Don-Il;Lee, Seong-Yim;Moon, Young-Hee
    • Korean Journal of Pharmacognosy
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    • v.27 no.3
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    • pp.184-189
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    • 1996
  • Ohyaksungisan, combined preparation of crude drugs, has been used for hemiplegia, arthralgia and paralysis in traditional Korean medicine. The anti-inflammatory activity of the aqueous extract from Ohyaksungisan(OSSE) was investigated on acetic acid-induced edema and adjuvant arthritis in rats. Acute toxicity and analgesic action in mice were also examined. Its anti-inflammatory activity on 5% acetic acid-induced edema and adjuvant arthritis was observed with oral administration. The acute toxicity showed 10% mortality at 2400 mg/kg (p.o), but was not showed at 1200 mg/kg (i.p). OSSE was showed to have significant analgesic action (P<0.05) at 150 mg/kg and this action was strengthened at 300, 600 mg/kg. The anti-inflammatory effect was showed significant preventive effect on the hind paw edema from 90 min. and the adjuvant arthritis, when orally administered for 19 days. showed significant inhibitory effect on the hind paw edema from the 5th day.

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Study on Acute Toxicity of Fermented Ohyaksungi-san (Wuyaoshunqi-san) Extracts (오약순기산 발효물의 급성 독성에 관한 연구)

  • Lee, Ji-Hye;Kwak, Dong-Hoon;Kim, Tae-Soo;Ma, Jin-Yeul
    • Journal of Korean Medicine for Obesity Research
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    • v.11 no.2
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    • pp.25-32
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    • 2011
  • Objectives: The purpose of this study was to investigate the acute toxicity with oral administration in ICR mice of Ohyaksungi-san fermented with Lactobacillus sp. Methods: In single oral administered toxic test, four groups were administrated different dosages(0, 1250, 2500, 5000 mg/kg) of fermented Ohyaksungi-san. After single oral administration, we observed number of death, clinical signs, body weight changes, hematological values and autopsy. Results: Compared with the control group, we could not find any toxic signs in the mortalities, clinical signs, body weight changes, necropsy findings and hematological values in all treated groups (1250, 2500 and 5000 mg/kg). Conclusions: These results indicate that the $LD_{50}$ value of fermented Ohyaksungi-san extract might be over 5000 mg/kg and it have no side toxic effect to ICR mice

Neuroprotective Activity of Fermented Oyaksungisan (오약순기산 발효물의 성분 분석 및 뇌세포 보호 활성)

  • Weon, Jin-Bae;Ma, Jin-Yeul;Yang, Hye-Jin;Ma, Choong-Je
    • Korean Journal of Pharmacognosy
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    • v.42 no.1
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    • pp.22-26
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    • 2011
  • Korean traditional prescription, Oyaksungisan was usually used to treat rheumatoid arthritis, paralysis. In this study, we compared the contents of five major components, ephedrine HCl, ferulic acid, hesperidin, 6-gingerol, glycyrrhizin and various unknown compounds in Ohyaksungisan (OY) with fermented Ohyaksungisan (FOY) by established HPLC-DAD method. The neuroprotective activity against glutamate-induced cytotoxicity in HT22 cell of OY and FOY was investigated by MTT assay. And anti-oxidative activity of OY and FOY was measured by DPPH radical, hydrogen peroxide and hydroxyl radical scavenging activity. After fermentation, contents of five major components and some unknown compounds (1), (2), (3) and (4) were increased in FOY. Also, FOY showed more potent neuroprotective activity. Furthermore, anti-oxidative activity of FOY was increased. In conclusion, the fermentation of OY could change contents various compounds and improved its neuroprotective activity.

Simultaneous Analysis of Six Constituents in Oyaksungi-san using HPLC-PDA (HPLC-PDA를 이용한 오약순기산 중 6종 성분의 동시분석)

  • Seo, Chang-Seob;Kim, Jung-Hoon;Shin, Hyeun-Kyoo
    • Herbal Formula Science
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    • v.20 no.2
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    • pp.37-46
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    • 2012
  • Objectives : Oyaksungi-san(Wuyaoshunqisan) has been used for treatment of stroke and rheumatoid arthritis in Korea. In this study, a simple and accurate high-performance liquid chromatography(HPLC) method was established for simultaneous determination of six main components, liquiritin, ferulic acid, naringin, hesperidin, neohesperidin, and glycyrrhizin in Oyaksungi-san, a traditional Korean herbal prescription. Methods : The analytical column for separation of six constituents was used a Gemini $C_{18}$ column maintained at $40^{\circ}C$. The mobile phase consisted of two solvent systems, 1.0% (v/v) acetic acid in $H_2O$ (A) and 1.0% (v/v) acetic acid in acetonitrile (B) by gradient flow. The flow rate was 1.0 mL/min and the detector was a photodiode array (PDA) set at 254 nm for glycyrrhizin, 280 nm for liquiritin, naringin, hesperidin, and neohesperidin, and 320 nm for ferulic acid. Results : Calibration curves were acquired with $r^2$ values ${\geq}0.9998$. The results of recovery test were 91.58%-105.90% with a relative standard deviations (RSDs, %) value less than 2.0%. The values of RSD for intra- and inter-day precision were 0.03%-1.72% and 0.03%-1.63%, respectively. The contents of the six compounds in Oyaksungi-san were 0.33-9.30 mg/g. Conclusions : The newly established HPLC method will be helpful to improve quality control of Oyaksungi-san.

The Effects of Ohyaksungi-san(Wuyaoshunqi-san) and Electrical Acupuncture on Nerve Regeneration after Crush Injury in Rat Sciatic Nerve (흰쥐 좌골신경 압좌 손상 후 오약순기산 및 전침이 신경손상의 회복에 미치는 영향)

  • Yang, Mi-Sung;Shin, Mi-Suk;An, Hye-Lim
    • Journal of Korean Medicine Rehabilitation
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    • v.18 no.4
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    • pp.25-37
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    • 2008
  • Objectives : Ohyaksungi-san(Wuyaoshunqi-san) has been used for many years as a treatment for sciatica in oriental medicine. And electrical acupuncture also has been used as a treatment for sciatica in recent study. By the way, it is hard to find the study that apply two treatments in the same time. This study was designed to evaluate the effects of Ohyaksungi-san(Wuyaoshunqi-san) and electrical acupuncture on nerve regeneration after crush injury in rat sciatic nerve in the same time. Methods : This study was designed with 4 subgroups to evaluate the effects of Ohyaksungi-san(Wuyaoshunqi-san) and electrical acupuncture on nerve regeneration. As control groups, group I has not been treated during 3 weeks after crush injury in rat sciatic nerve. Group II has been treated Ohyaksungi-san(Wuyaoshunqi-san), Group III has been treated electrical acupuncture, and Group IV has been treated both during 3 weeks. This study has been estimated sciatic function index and change of GAP-43 immunoreactivity about sciatic nerve regeneration. Results : 1. The test for nerve regeneration had significantly good result of sciatic function index in the experimental groups as compared with control groups(p<.05). Especially, Group IV has the greatest result of sciatic function index. 2. GAP-43, the marker of nerve regeneration, more increased in the experimental groups as compared with control groups. Especially, Group IV has the greatest result of GAP-43. Conclusions : Ohyaksungi-san(Wuyaoshunqi-san) and electrical acupuncture have good effects on nerve regeneration after crush injury in rat sciatic nerve.

Effects of Ohyaksungi-san(Wuyaoshungi-san)and Jungsongouhyul Pharmacopuncture on Pain Reduction and Nerve Regeneration after Crush Injury in Rat Sciatic Nerve (오약순기산(烏藥順氣散) 및 중성어혈(中性瘀血) 약침(藥鍼)이 흰쥐 좌골신경 압좌 손상 후 통증 감소와 신경 재생에 미치는 영향)

  • Jung, Moon-Jae;Lee, Jeong-Han;Yeom, Seung-Ryong;Lee, Su-Kyung;Song, Yung-Sun;Kim, Ki-Byoung;Kwon, Young-Dal
    • Journal of Korean Medicine Rehabilitation
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    • v.19 no.2
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    • pp.51-72
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    • 2009
  • Objectives : This study was designed to evaluate the effects of Ohyaksungi-san(Wuyaoshungi-san) and Jungsongouhyul pharmacopuncture on pain reduction and nerve regeneration after crush injury in rat sciatic nerve. Methods : Animal model was produced through crush injury of right sciatic nerve and they were divided into four groups; Group I: no treatment control group; Group II: experimental group treated with Ohyaksungi-san(Wuyaoshungi-san); Group III: experimental group treated with Jungsongouhyul pharmacopuncture; Group IV: experimental group treated with Ohyaksungi-san(Wuyaoshungi-san) and Jungsongouhyul pharmacopuncture. For the assessment of pain, this study was observed the paw withdrawal latency(PWL) and immunoreactivity on the substance-P. For the assessment of nerve regeneration, the sciatic functional index(SFI) and immunoreactivity on the BDNF were measured. Results : 1. In the assessment of pain, the PWL of experimental groups was significantly higher than control group and group IV was significantly higher than other groups at the all days. 2. In immunohistochemical response of substance-P, as time passes, the immunoreactivity of all groups were decreased gradully. Especially, group IV had the lowest immunoreactivity. 3. In the assessment of SFI, the SFI of experimental groups were significantly higher than control group. 4. In immunohistochemical response of BDNF, the BDNF immunoreactivity of all groups was significantly higher than control group and especially, group IV had the highest immunoreactivity at the 14 days after injury. 5. H & E stain was used on the liver and kidney to investigate toxic effect of Jungsongouhyul pharmacopuncture and Ohyaksungi-san(Wuyaoshungi-san) on on 21 days after injury. However there were no any toxic effects both control group and experimental groups. Conclusions : On the basis of these results, we propose that Ohyaksungi-san(Wuyaoshungi-san) and Jungsongouhyul pharmacopuncture were related to pain reduction and motor nerve recovery, also decreased substance-P expression and increased BDNF expression after crush injury of sciatic nerve, especially these two treatments could be more effective when they were combined simultaneously.

The Effects of Ohyaksungi-san(Wuyaoshunqi-san) on Cognition and Motor Function Recovery after Ischemic Brain Injury in Rat (오약순기산(烏藥順氣散)이 허혈성 뇌손상 흰쥐의 인지 및 운동기능회복에 미치는 효과)

  • Ryu, Ki-Jun;Choi, Jin-Bong;Shin, Mi-Suk;Kim, Sun-Jong
    • Journal of Korean Medicine Rehabilitation
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    • v.18 no.3
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    • pp.1-17
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    • 2008
  • Objectives : Ohyaksungi-san(Wuyaoshunqi-san) has been used for many years as a treatment for cerebrovascular diseases in Oriental medicine. This study was designed to evaluate the effects of Ohyaksungi-san(Wuyaoshunqi-san) on cognition and motor function recovery after ischemic brain injury, and also the expression of BDNF in hippocampus. Methods : This study was designed with 4 subgroups to evaluate the effects of Ohyaksungi-san(Wuyaoshunqi-san). As control groups, group I has no treatment during 1 week after ischemic brain injury and group II has no treatment during 2 weeks after ischemic brain injury. As experimental groups, group III has been treated with Ohyaksungi-san(Wuyaoshunqi-san) during 1 week after ischemic brain injury and group IV have treated with Ohyaksungi-san(Wuyaoshunqi-san) during 2 week after ischemic brain injury. Each group has been examined by tests as follows, neurological motor behavioral tests, cognitive motor behavior test and histological test. Neurological motor behavior tests consisted of limb placement test, beam-walking test and horizontal wire test. Cognitive motor behavior test was performed by using Morris water maze. In the histological test, TTC(2,3,5-triphenylteterazolium chloride) staining, hematoxylin & eosin staining, and immunohistochemical staining were used. Results : 1. The tests for motor function recovery change had significantly good result in the experimental groups as compared with control groups(p<.05). 2. The Morris water maze test on cognition also had significantly good result in the experimental groups as compared with control groups(p<.05). 3. In the immunohistochemical staining for the expression of BDNF in hippocampus, more immune reaction was investigated in the experimental groups as compared with control groups. Especially group IV has the greatest immune reaction. Conclusions : Ohyaksungi-san(Wuyaoshunqi-san) has good effects on cognition and motor function recovery after ischemic brain injury, and also the expression of BDNF in hippocampus.