• Food Science of Animal Resources
• /
• v.28 no.2
• /
• pp.154-159
• /
• 2008

To investigate the rheological properties of protein mixed gels mediated by microbial transglutaminase (MTGase), pork myofibrillar protein (MFP), sodium caseinate (SC) and their mixture (MS), the various gels were incubated at different temperatures for various times. Extracted MFP, SC and their mixture (MS, 1:1) were incubated at different temperatures ($4^{\circ}C$ vs $37^{\circ}C$) for various times (0, 0.5, 2, 4 hr), and assessed for viscosity, gel strength and other characteristics using differential scanning calorimeter (DSC) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). DSC measurements showed that incubation at $37^{\circ}C$ rather than $4^{\circ}C$ caused marked changes in thermal transition, and MS displayed similar thermal curves (three endothermic transitions) to MFP and SC alone. After incubation at $37^{\circ}C$ for 2 hrs, the viscosity (cP) of MS increased (p<0.05) due to induction by MTGase, whereas no differences were observed at $4^{\circ}C$. However, gel strength values were no different, regardless of incubation temperatures and times. Future research will address how longer incubation times affect the functionality of protein mixed gels mediated by MTGase.

• Food Science of Animal Resources
• /
• v.28 no.5
• /
• pp.660-666
• /
• 2008

This study was designed to compare the thermal oxidative stability of lard, soy bean oil, and hot pepper seed oil for 0-3 d at $100^{\circ}C$, and to evaluate the effect of capsaicin on thermal oxidative stability in lard and soy bean oil. As result, thermal oxidation stability was shown in the order hot pepper seed oil>soy bean oil>lard for 0-3 d at $100^{\circ}C$. In blended oils, hot pepper seed oil effectively inhibited lipid oxidation when mixed with lard than soy bean oil by showing the ratio of 30% pepper seed oil plus 70% lard and 60% pepper seed oil plus 40% soy bean oil inhibited lipid oxidation during storage periods. And to investigate the antioxidative effect of antioxidants such as capsaicin and alpha-tocopherol in hot pepper seed oil, 1,200 and 2,400 ppm capsaicin, or 0.3% alpha-tocopherol were added in soy bean oil and lard and stroed for 0-3 d at $100^{\circ}C$. Capsaicin inhibited lipid oxidation in lard but not in soy bean oil, however alpha-tocopherol exhibited a prooxidaton effect in soybean oil. Therefore, it suggests that the application of hot pepper seed oil or capsaicin in lard may be better for thermal oxidative stability.

• Food Science of Animal Resources
• /
• v.25 no.1
• /
• pp.78-83
• /
• 2005

Purge & Trap method was applied to perform more simple and rapid detection for analysis of volatile flavor compounds in milk. Maximal sampling of 30 mL milk for glass flask sparger was treated by He gas purging for 2 hours. Reported major volatile compounds were detected by GC-MS after 2 hours absorption and desorbed from Purge & Trap equipped with Tenax trap. Volatile flavor compounds were analyzed by Purge & Trap and GC-MS to investigate the changes of flavor components in milk between raw and deodorized milk. Fourteen volatile compounds including acetaldehyde, ethanol, 2-propanone, dimethyl sulfide, isobutanal, 3-methyl 2-butanone, 2-butanone, 3-methyl butanal, pentanal, 3-hydroxy-2-butanone, methyl disulfide, hexanal, and 2 others were detected. Six compounds such as ethanol, dimethyl sulfide, pentanal, 3-hydroxy-2-butanone, and methyl disulfide were completely eliminated after deodorization treatment. Four compounds such as 3-methyl 2-butanone, 2-butanone, 3-methyl butanal, and an unknown compound 81 (M/sup +/) were also decreased after raw milk was deodorized. The other four compounds such as acetaldehyde, 2-propanone, hexanal, and an unknown compound (M/sup +/) were not decreased.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.20 no.9
• /
• pp.255-261
• /
• 2019

This trial was conducted to determine the optimal supplementation level of Hermetia illucens (HI) on the growth performance, the apparent total tract retention of nutrients and the economic efficiency in weaning pigs. A total of 192 weaned pigs ($Landrace{\times}Yorkshire{\times}Duroc$; $6.51{\pm}0.15kg$) were randomly allotted to 4 treatments and 6 replicates on the basis of their initial body weight. The dietary treatments included a corn-soybean meal based diet supplemented with 0, 1, 2 or 3% HI. There were no significantly effects between the treatments in phases I, II and overall. But average daily gain was significant and linearly increased when HI was added to the diet in the overall group. Although digestibility of dry matter (DM) was increased by adding HI, digestibility responses were not observed by supplying HI in phase I. Also, all the parameters of economic efficiency were not significantly different according to the level of HI. The results showed that HI had beneficial effects on growth performance and digestibility of dry matter. However, there were no effects on economic efficiency by using HI in weaning pig diets.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.41 no.1
• /
• pp.35-40
• /
• 2021

This study was conducted to evaluate the forage productivity and feed value of Sasa borealis (S. borealis) using growing black goats(S. borealis) in order to improve the utilization of S. borealis and to help mitigate the problem of reduced plant species diversity caused by S. borealis in Hanlla Mountain. One control and three treatments were made by the level of addition of S. borealis silage to the TMR feed. T1(10%), T2(20%), and T3(30%) treatments showed more daily weight gains than control group. Feed conversion ratio of T2 is 4.4g, which is significantly lower than control(P<0.05). The nitrogen retention in the control, which had relatively high dry matter intake, was 12.5g, which was significantly higher than that of T3. Sasa. borealis silage is considered to be able to use as a forage source for black goats, and if it is fed in an appropriate amount, it is considered that it will help improve livestock productivity, such as weight gain and feed conversion ratio.

• Journal of Animal Science and Technology
• /
• v.44 no.6
• /
• pp.801-808
• /
• 2002

Proteolytic activities of some commercial milk clotting enzymes(rennet, trypsin, pepsin, papain W-40, neutrase 1.5 and protease S) in bovine skim milk containing 0.02% $CaCl_2$ were determined by measuring DH(Degree of Hydrolysis), NPN(Non Protein Nitrogen) and by comparing patterns of SDS-PAGE(Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis). The DH of microbial enzymes(neutrase 1.5 and protease S) and trypsin in bovine skim milk were higher than those of pepsin and papain W-40. The amounts of NPN in the milk treated with trypsin and the other animal enzymes(rennet and pepsin) showed the highest and lowest degrees of proteolysis, respectively. SDS-PAGE showed that trypsin and protease S hydrolyzed $\alpha$-lactalbumin and papain W-40 hydrolyzed $\beta$-lactoglobulin slightly, while neutrase 1.5 hydrolyzed both $\alpha$-lactalbumin and $\beta$-lactoglobulin after treating for 90 min. Trypsin and protease S easily hydrolyzed ${\alpha}_s$-casein and $\beta$-casein, which were not hydrolyzed by rennet. Papain W-40 hydrolyzed $\kappa$-casein more than rennet as shown in SDS-PAGE. Based on the results of the experiments, the DH and NPN of trypsin, neutrase 1.5 and protease S were shown to be higher than those of the other enzymes. The SDS-PAGE patterns of papain W-40 and neutrase 1.5 were similar with that of rennet.

• 한국유가공학회:학술대회논문집
• /
• 2006.06a
• /
• pp.29-45
• /
• 2006

최근에 들어 웰빙(well-being)과 함께 로하스(LOHAS: Lifestyle of Health and Sustainability)란 개념이 급속도로 확산되고 있으며, 이를 만족하는 식품으로 친환경 농산물과 유기 가공 식품이 대두되고 있다. 여러 마케팅 조사에서도 친환경 농산물과 유기 가공 식품의 소비자 선호도는 상당히 높게 나타난다. 이러한 다양한 소비자의 요구에 맞춰 우리의 2세에게 더욱 안전하고 좋은 품질의 이유식을 제공하고 나아가 한계에 이른 '지구와 환경을 해치지 않는 지속가능 한 소비'를 유도하고자 업체들은 유기농 이유식을 개발하고 있다. 유기농 소비자 10명 가운데 7명은 최근의 웰빙 붐 때문에 유기농에 관심을 가지게 됐으며, 가격이 다소 비싸더라도 가족의 건강을 위해 친환경 농산물을 구입하는 것으로 나타났으며, 국내외를 막론하고 친환경 농산물과 유피 가공 식품의 시장 동향을 살펴보면 지속적으로 증가하고 있으나, 아직까지 일반 제품에 비해 미미한 수준이며, 국내는 인증 제도가 없으나, 유기 가공 식품표시기준에 따라 표시되고 있다. 일반적으로 이유식이라 함은 식품공전에서 영 ${\cdot}$ 유아용 곡류 조제식만을 말하지만 넓게 축산물의 조제유류, 영아용 조제식, 성장기용 조제식, 기타 영 ${\cdot}$ 유아식을 포함할 수 있으며, 유기농 이유식 제조 현황을 살펴보면 동결, 분무, 드럼 등의 건조와 퍼핑이나 Extruder를 통한 알파화로 원료를 가공하여 은 살균, 멸균, 균질, 혼합, 건조, 냉각, 포장 등의 다양한 공정으로 이루어진 분무건조나 유동층이나 연속식 과립기를 사용하여 분말이나 입자형 이유식을 생산하며, 동결 건조를 하여 열수를 가해 죽 상태로 먹을 수 있도록 한 제품, 데워서 떠 먹일 수 있도록 병조림이나 레토르트 유동식 제품, 바로 마실 수 있는 쥬스류, 두유류, 과자 등으로 생산하고 있다. 주된 메이저4사는 일동후디스, 남양유업, 매일유업, 파스퇴르유업으로 다양한 제품들을 생산하고 있으며, 두유를 위주로 한 정식품, 연세우유와 종근당건강, 다양한 다국적 기업들의 제품이 수입되고 있는 실정이다. 이유식 시장도 96년 하반기부터 정체를 보이는 상태다. 2002년도 이유식 시장은 약 1,300억원 규모로 업체들의 고가 신제품과 유기농 이유식 출시로 판매 금액은 소폭 상승하였으나 판매 수량은 큰 변동이 없다. 저출산에 따른 양육비 집중과 유기농 제품에 대한 저변이 크게 확산돼 베이비 웰빙은 어느 정도 예견된 일이거니와 이미 미국 등 선진국에선 유아에 그치지 않고 아동까지 유기농과 고급 원료를 사용한 고가의 웰빙 제품이 봇물을 이루고 있는 상황이어서 유아식 시장은 어느 때보다 경쟁이 치열할 것이며, 국내 식품 업체들이 너도나도 유기농 제품과 매장사업에 뛰어들고 있다. 유기 가공 식품의 지속적인 발전을 위해서 (1) 국내산/수입산 공통으로 인증 제도가 도입되고 원료 농산물뿐만 아니라, 모든 공정이나 공장에 대한 종합적 관리와 동일한 인증 표시가 이루어져야 한다. (2) 정부 부처의 이기를 타파할 수 있는 법정부적 기구나 대책이 필요하다. (3) 유기 가공 식품을 취급하는 업계에서는 기존 3%의 비의도적 혼입치의 고려나 상한선 재설정을 요구하고 진정한 유기 가공 식품으로 공정 경쟁한다. (4) 활발한 인증심사원의 교육과 배출로 인증이나 심사기관의 민간 이양을 적극 장려하고 국가관 상호 신뢰를 구축한다. (5) 각 소비자 단체나 소비자는 환경을 살리고 생명체의 공생이라는 관점에서 최선의 길이 유기농 제품임을 인식하고 소비하여 우리의 다음 세대에게서 빌려온 소중한 환경을 물려준다.

• Journal of Animal Science and Technology
• /
• v.49 no.3
• /
• pp.295-302
• /
• 2007

Leptin, the product of the obese(ob) gene, is an adipocyte-derived hormone for the regulation of whole- body energy storage and energy usage. It has been reported that the homozygous mutations in the gene for leptin(LEP) induce obesity and reduce energy expenditure. In cattle, LEP has significant roles directly or indirectly related with phenotypes such as body weight and fat deposits, therefore SNPs of LEP have been considered important genetic marker to estimate carcass fat content in cattle. In this study, SNPs were screened in LEP(2,222 bp) between intron 1 to 3'-UTR from 24 independent Hanwoo(Korean cattle) by PCR and DNA sequencing. Total 25 SNPs were found and two nonsynonymous SNPs including T1163A(V19E) and G3256A(G132D) were newly detected only from Hanwoo. Among 20 SNPs previously reported in cattle, 16 SNPs were found in Hanwoo; however, the frequencies of some SNPs were significantly different between Hanwoo and western cattle breeds. The other 4 SNPs were not detected from Hanwoo. These Hanwoo specific SNP patterns in LEP will be used in development of molecular marker and application to genetic improvement of Hanwoo.

• Journal of Animal Science and Technology
• /
• v.47 no.3
• /
• pp.355-362
• /
• 2005

This study was carried out to obtain basic information on variety selection for the utilization of whole crop rices(WCR) at National Institute of Crop Science, RDA, in 2004. Fifteen varieties and elite line were evaluated on feed value such as dry matter yield(DMY), crude protein( CP), acid detergent fiber(ADF), neutral detergent fiber(NDF) and total digestible nutrients(TDN). The dry matter yields were ranged from 13.23 to 17.83 ton per ha, the highest yielding varieties were Sobibyeo(l6.98ton / ha) in Japonica type, SR22060 (17.83 ton / ha) in New plant type, Hangangchalbyeo(I7.66 ton / ha) in Tongil type. Suweon 468 showed the highest value in the RFV and TDN content among the varieties, and Suweon 468, Suweon 498, Suweon 490 and SR22058 were chosen to have the high feed values through cluster analysis. The dry weight(grain) was found to be positively related with percent of the ripened grain, 1,000 grain weight and CPo TDN content was found to be positively related with CP, but negatively related with NDF and ADF. RFV was found to be negatively related with plant height, NDF and ADF. The promising rice varieties for WCR were Suweon 468, Suweon 498, Suweon 490 and SR22058 on the basis of CP, TDN and DMY.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2006.05a
• /
• pp.1098-1101
• /
• 2006

최근 3대강특별법에 의하여 시행되고 있는 환경부의 수질오염총량관리를 위해 서낙동강 유역인 낙본N 단위유역에 대한 해당 지자체의 기본계획과 시행계획이 수립된 바 있다. 낙본N 단위유역은 서낙동강을 중심으로 좌측의 대부분이 경상남도 김해시이고, 우측과 하류부는 부산광역시 강서구이다. 서낙동강은 평상시에는 주요 농업용수 공급원과 철새도래지로서 홍수시에는 거대한 유수지 역할을 하고 있다. 서낙동강 유역내에는 분뇨처리장, 하수처리장, 농업 및 축산활동, 내수면 양식어업, 오염원 배출공장 등의 점, 비점오염원이 있으며 대저수문과 녹산수문에 의해 조절되는 정체수역으로서 오염부하강도가 높은 유입지천들로 인하여 상습적인 녹조 발생수역으로 수질개선의 필요성이 매우 높은 지역이다. 본 연구에서는 대표 유역으로 낙본N유역의 소유역 중 배출부하량이 가장 큰 지류에 해당하는 조만강 유역인 낙본N06 소유역의 오염원 조사결과를 이용하여 오염부하량 산정기법을 검토하였다. 장래 오염부하량 예측은 자연증가, 개발계획, 삭감계획에 의해 산정하는데, 이와 같은 과정에서 과거 오염원조사, 부하량산정, 폐수 배출부하량 모니터링 과정에서의 불확실성이 따르게 된다. 본 연구에서는 이와 같은 오염부하량산정 과정에서의 정확도를 높이고자 오염원 자료의 증감에 따른 오염부하량 산정결과의 민감도 분석을 통하여 신뢰도 평가를 수행하였다. 방대한 오염원자료를 이용한 오염부하량 산정은 한국환경정책평가연구원(KEI)에서 개발한 데이터베이스관리 프로그램(Access Program)을 이용하였으며, 각 오염원별 오염원 현황 및 전망 결과와 환경부의 수계오염총량관리기술지침에서 제시한 각 오염원별 오.폐수발생원단위, 배출원단위, 전환계수, 배출계수 등을 이용하여 각 오염원별 배출부하량을 산정하였다. 본 연구에서는 오염원 조사나 장래 오염원 예측에서 있을 수 있는 오차에 대한 전체 배출부하량의 변화를 살펴봄으로써 방대한 양의 오염원 조사시에 정확성 및 효율성을 높이고자 하였다. 본 연구의 결과를 통해 할당부하량을 개별 오염원별로 할당하고 적정한 개발계획과 실현가능한 삭감계획 및 이행방안을 수립하기 위한 오염원조사를 수행함에 있어 기초자료의 효율적인 관리를 통해 오염부하량 산정의 정확성을 높이고자 한다.