• Title/Summary/Keyword: 영동지방

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Ovarian and Fat Body Yolk Protein Synthesis in Culex piplens pallens (홍모기(Culex pipiens pallens) 지방체와 난소에서의 난황단백질합성에 관한 연구)

  • 이승훈;박영민;성기창
    • The Korean Journal of Zoology
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    • v.36 no.3
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    • pp.416-424
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    • 1993
  • Ovarian Yolk protein (YP2) synthesis has been investigated in mosquito, Culex pipiens pallens. Yolk protein amount which was syntheized in fat body, accumulated into ovary were analyzed by Rocket immunoelectrophoresis and in vitro organ culture. The result was that yolk protein synthesis began to occur at 6hrs after blood meal, reached at maximum level by 24hrs, and was completed within 48hrs. Yolk protein accmulation into the ovary began to start at 6hrs and coutinued for up to 60hrs after blood meal. Extract from 0, 24, 48, 72hrs ovaries after blood meal were analyzed by electrophoresis and Western blotting. The result was that 24hrs ovary contain one yolk protein(YP1), and 48, 72hrs ovaries contain two kinds of yolk proteins(YPl and YP2). When 48hr ovaries and fat bodies were incubated in $^3$H-leucine contained medium, protein synthesis was not occurred in fat body, but ovary synthesized much protein contained yolk protein (YP2). The result of crossed immunoelectrophoresis represented the same immunity between YPl and YP2. The present data suggest that ovary synthesize yolk protein(YP2) in mosquito, Culex pipiens pallens.

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Composition of Lipids Associated with Dense Coat-enriched Fractions of Bovine Milk Fat Globule Membrane (우유 지방구막의 고밀도 표피에 결합된 지질의 조성)

  • Kwak, Hae-Soo;Chung, Choong-Il;Lee, Jae-Young
    • Korean Journal of Food Science and Technology
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    • v.21 no.5
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    • pp.726-734
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    • 1989
  • To analyze the components of dense coat fractions associated with fat globule membrane, The membrane was treated with various concentrations of Triton X-100, a non-ionic detergent, and the composition of lipids associated to the detergent insoluble material was analyzed. The amount of protein, phospholipid, cholesterol and ganglioside in milk fat globule membrane was reduced consistently with increasing concentrations of Triton X-100. Butyrophilin (band 12), xanthine oxidase (band 3) and band 16 as constituents of insoluble coat materials was revealed after electrophorisis on SDS-polyacrylamide gels. Phosphatidyl ethanolamine, phosphatidyl choline, phosphatidyl serine, phosphatidyl inositol and sphingomyelin were identified as the major phospholipids of the coat materials without selective concentration relative to the original membranes. Percentages of total phospholipid were not changed by any of the treatments. Fatty acids of total lipid were myristate, palmitate, stearate (major saturated acids), oleate and linoleate (major unsaturated acids). Cholesterol contents on a protein basis were slightly reduced with increasing concentrations of Triton X-100. Cholesterol adhered to protein more tightly than other constituents The contents of gangliosides was proportionally refuted with increasing concentration of Triton X-100.

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The Effects of Climatic Factors on the Tree Ring Growth of Pinus densiflora (기후인자가 Pinus densiflora의 연륜 생장에 미치는 영향)

  • Lee, Sang-Tae;Park, Mun-Seop;Jun, Hyang-Mi;Park, Jin-Young;Cho, Hyun-Seo
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.10 no.4
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    • pp.177-186
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    • 2008
  • This study was carried out to analyze the relationship between major climatic factors (temperature and precipitation) and tree ring growth of Pinus densiflora in 28 cities and counties in Korea. To examine tree ring growth of Pinus densiflora and the response of the climatic factors to climate change, the regional climatic data (for how many years??) from weather stations were categorized by five groups using cluster analysis for major climatic factors. In terms of relationship between monthly average temperature and tree ring growth of Pinus densiflora, negative correlations were observed for the temperatures in March of the present year in Baekdudaegan region and those in February and March in Gyeongbuk and East coast region. In addition, the temperature in October of the previous year was also negatively correlated with tree ring growth. Precipitation was positively correlated with the April and June temperatures of the present year in Baekdudaegan region and Gyeongbuk, respectively. Precipitation effect on the tree ring growth with current year June in Honam and Gyeongnam inland region and June, September in East coast region.

Studies on the Effects of Copper on the Lactate Dehydrogenase and Esterase Isozymes in Various Tissues of Carassius carassius (붕어(Carassius carassius)의 조직내 젖산수소이탈효소와 에스테라아제 아이소자임에 미치는 동의 영향에 관한 연구)

  • Lee, Choon-Koo;Choo, Il-Young
    • The Korean Journal of Zoology
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    • v.16 no.2
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    • pp.79-96
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    • 1973
  • In order to elucidate the effects of copper on Corassius carassius, the following were studied: 1) lactate dehydrogenase isozyme patterns by cellulose acetate electrophoresis, 2) LDH activity and copper effect on LDH enzyme system y spectrophotometry, 3) esterase isozyme patterns by agar thin layer electrophoresis, 4) hemoglobin patterns by starch gel electrophoresis, and 5) histological study. 1. There were two bands of LDH isozymes (LDH-3 and LDH-5) in the gill, three bands (LDH-2, LDH-4, and LDH-5) in the liver, and two bands (LDH-3 and LDH-4) in the muscle of the normal fish. The LDH-1 bond was not found in the above three tissues. When the fish were exposed to copper, LDH-3 appeared in the liver, LDH-5 in the muscle, but no new LDH band appeared in the gill. 2. The sepcific activities of the LDH were lowest in the gill and highest in the muscle of the normal fish, and they were gradually decreassed in the gill and highest in the muscle of the normal fish, and they were gradually decreased in the liver and mucle except in the gill from 1-day to 10-day exposure to copper. It indicates that LDH activities in the liver and muscle of the fish were inhibited by copper. 3. Through in vitro experiment, it is clear that the decrease of the LDH activities of the liver and muscle of the fish exposed to copper is mainly caused by the inhibition on the M-LDH in the fish. 4. The numbers of the esterase isozyme bands of the gill, liver, muscle, blood, brain, and kidney of the normal fish were 3, 6, 2, 2, 2, and 2 respectively, and these numbers were the same as those exposed to copper. The relative mobilities of the esterase bands in the gill, liver, blood, and kidney of the exposed group were different from those of the control. 5. There was one hemoglobin band on the anode in the normal fish. It seems that the nobility of hemoglobin band of the fish exposed to copper was slightly faster than that of the normal fish. 6. The normal gill lamellae of the fish consisted of centrally located pillar cells and a number of mucus cells. When the fish were exposed to copper, the epithelial layer was divorced first, disintegrated, and then destroyed completely. 7. The liver of the normal fish had prominent central veins, cords of hepatic cells, and sinusoids. When the fish were exposed to copper, numerous droplets of fat appeared in the cells around the central vein of the liver. It is assumed that the fatty droplets were accumulated by the lesion due to fatty metamorphosis of the liver caused by copper. 8. There was no histological difference between the muscle of the normal fish and that of the fish exposed to copper. 9. In the normal fish, the tubules of the kidney were surrounded by hemopoetic tissues. However, the kidney tissue of the fish exposed to copper received some damage on the proximal tubules. Since the tubule cells were reduced in height, the lumens of the tubules were enlarged. Consequently many proximal tubules exhibited some pink-stained granular casts and various stages of degeneration.

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Districting the Growth Zone by Diameter Growth Pattern for Pinus densiflora in Kangwon Province (직경생장(直徑生長)패턴에 따른 강원도(江原道) 소나무의 생장권역(生長圈域) 구분(區分))

  • Song, Chul Chul;Byun, Woo Hyuk;Lee, Woo Kyun
    • Journal of Korean Society of Forest Science
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    • v.84 no.1
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    • pp.71-76
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    • 1995
  • This study on Pinus desiflora in Kangwon Province has been performed for the purpose of classifying its growth area by geographical factors. To classify its growth area, the basic data which had were the measured values for annual ring width from cores were used. Individual variations in the measured values were removed through the standardization. Regional mean chronologies were estimated from the standardized values. The growth area was classified by the cluster analysis on the basis of the regional mean standardized indices. The results of this study shown that annual growth patterns to be clustered similar to geographical distribution in Kangwon Province. And the regional variations of annual growth patterns in the western part of Kangwon province were greater than those in the eastern part of Kangwon province.

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경주 월성 해자 및 일정교지 출토 고목재의 수종과 연륜연대 분석

  • Kim Tae-U;Park Won-Gyu;Jeong Yeong-Dong
    • 한국문화재보존과학회:학술대회논문집
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    • 2005.11a
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    • pp.290-295
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    • 2005
  • 본 실험은 경주 월성해자와 일정교지에서 발굴된 고목재를 시료로 사용하였다. 월성 해자 5점, 일정교지 2점으로 총 7점을 대상으로 수종분석과 연륜연대 해석을 실시하였다. 수종분석은 광학현미경을 사용하여 식별을 하였으며, 연륜연대 해석은 연륜측정후 연륜분석 컴퓨터프로그램과 그래프 일치도를 육안으로 비교하여 실시하였다. 7개의 시료 전부 밤나무였다. 밤나무는 현재 평남과 함남 이남에 분포하는 수종으로 특히 따뜻한 남부지방에 살기 적합한 수종이다. 각 시료간의 상대연도를 확인한 결과는 월성해자 목재의 수령은 $31{\sim}37$년 일정교지는 45년으로 확인 되었다. 월성해자의 각 시료들은 동시대이나 벌채기간은 6년 차이가 났다. 일정교지의 시료에서는 일치성을 확인하지 못했다.

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A Study on the Type of Folk House in YongDong-South disticts Gangwon-do - Focused on the SimPori in DoGae - (강원 영동남부지방 민가에 관한 연구 - 도계읍 심포리(深浦里)를 중심으로 -)

  • Rim, Sang-Kyu;Kim, Yong-Gee;Chai, Hee-Jai
    • Journal of the Korean Institute of Rural Architecture
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    • v.9 no.3
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    • pp.23-31
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    • 2007
  • The objective area of this study is a private house of common people at SimPori in the riverside area of OsipCheon. This area is the upriver of OsipCheon, and it was distributed at the 田-shaped House and the Dureong House with the composition of GuDl. Owing to the frequent interchange of AnDong and BongWha in the Young Seo district, the Kyup House and the SaBang House mixed with the 田-shaped House with GuDl of the YoungDong district. So the form of Folk House at this area is Dureong House. The Folk House of the seaside of SamCheok City is a compromised type among the 田-shaped House of GuDl in the north district and with MaRu in the south district, and introduced an indeterminate MaRu formed in the GaGokCheon area.

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A Research of the management and the technical problems to strengthen Competitive Power of Small-medium Manufactures in south Area of the Chung-buk (충북 남부지역 중소 제조기업의 경쟁력 제고를 위한 경영 및 애로기술 실태조사 연구)

  • 윤상원;구경완;정성진;백기영;김선훈;서용성;이근복
    • Proceedings of the KAIS Fall Conference
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    • 2001.05a
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    • pp.66-70
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    • 2001
  • 본 연구에서는 충북 남부 3개군 지역(옥천ㆍ보은ㆍ영동)의 중소 제조기업의 경영실태 및 애로기술을 대상으로 실증조사 되었다. 지역의 특정상황의 전제 하에 지방 중소기업들의 실질적인 문제점들을 발견하고, 그 개선 방안을 탐색함은 물론 산ㆍ학ㆍ연 컨소시엄 사업의 성공적인 수행을 위해 시도되었다. 세부적으로는 중소 제조기업의 경영 및 애로기술 특성을 일반적 사항, 경영애로요인, 경영전략, 현장개선기법, 정보화, 제품 및 공정 기술상의 애로기술 현황파악의 6개 영역으로 조사되었다. 조사결과들은 추후 산ㆍ학ㆍ연 공동기술개발 사업에 있어 창의적, 틈새적 과제발굴 및 신제품 기술개발의 토대마련이 될 뿐 아니라, 지역경제 발전을 위한 산ㆍ학ㆍ관의 지원 전략 수립에 적극 활용 될 것으로 판단된다.

Improvement of River Revetment in Kangwon (강원도 하천호안공법 개선방안)

  • Park, Sang-Deog;Choi, Han-Kyu;Choi, Jong-In;Choi, Heung-Sik;Hong, Jong-Sun
    • Proceedings of the Korea Water Resources Association Conference
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    • 2009.05a
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    • pp.485-490
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    • 2009
  • 강원도의 약 89%가 산지로 되어 있기 때문에 강원지역 주민의 거주지는 대부분 홍수 피해를 강하게 받는 계곡이나 하천변에 잡고 있으며 매년 반복되는 홍수의 피해 위험이 높다. 따라서 홍수 시 생활공간을 보호하기 위한 제방의 안전성을 확보하는 것은 강원도의 하천관리에서 가장 중요한 점이다. 호안공법은 일반적으로 하천의 생태계나 경관적인 측면에서 부정적인 경우가 많은 인공시설물이기 때문에 최근에는 자연과 어울리는 친자연형 호안공법을 추구한다. 본 연구는 강원도 내 1개 국가하천 6개 지점, 214개 지방하천 2,760개 지점에 대한 하천 호안공법의 종류, 식생상태, 하천특성, 호안파괴 특성 등을 조사하고 하천호안공법 적용 하천특성과 호안공법, 식생 등의 관계를 분석하여 강원도형 호안공법 개발의 기초자료를 제공하는 데 목적이 있다. 강원도에 설치된 호안공법은 망태형, 붙임형, 블록형, 쌓기형, 옹벽형으로 구분할 수 있으며, 영동지역은 망태형 25%, 옹벽형 20.1.%, 블록형 18.3%를 차지하고 있으나 영서지역 하천은 쌓기형 32.9%, 망태형 19.2%, 옹벽형 16.6%를 나타내고 있다. 두 지역에서 옹벽이 16%이상을 차지하는 것은 하천경사가 급하고 만곡 수충부가 많아 홍수 시 구조적인 안정성이 큰 옹벽을 통한 호안보호에 따른 것으로 판단된다. 옹벽을 제외하면 하천평면이 호안공법 선택에 큰 영향을 미치지 않은 것으로 보이며 오히려 하천경사나 하천재료 특성이 반영된 것으로 판단된다. 호안파괴의 원인은 홍수 시 하상의 세굴이다. 그러나 옹벽호안은 국부적인 하상세굴에도 콘크리트 구조특성으로 쉽게 파괴되지는 않는 것으로 판단된다. 보 하류지점에 설치된 돌망태 호안은 하상저하와 보에 의한 유향변동으로 발생된 강한 수충현상에 의해 파괴되는 것으로 조사되었다. 호안의 식생 활착은 블록형, 망태형, 쌓기형 호안의 순으로 양호한 것으로 나타났다. 망태형이나 쌓기형 호안은 영동지역보다는 영서지역에서 높은 식생 피복도를 나타냈으며, 이는 하천의 경사와 호안의 침수빈도와 관계가 큰 것으로 판단된다. 식생블록형 호안은 지역 구분 없이 식생피복도가 높고 하천경사나 재료특성에도 불구하고 강원도의 하천호안공법으로 우수한 기능을 한다. 시급히 식생을 도입하고자 하는 경우 식생블록형 호안이 추천되며 호안공의 안정성과 식생의 다양성 측면에서는 망태형 호안공법이 추천된다.

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On the Occurrence and Distribution of Storage Proteins During the Metamorphosis of Bombyx mori L (누에의 變態에 따른 貯藏蛋白質의 出現과 分布에 관하여)

  • Eul Won Seo;Hak Ryul Kim
    • The Korean Journal of Zoology
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    • v.29 no.1
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    • pp.1-12
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    • 1986
  • Electrophoretic, immunological, and column chromatography methods were used to determine the appearance and distribution of storage proteins in various organs during the metamorphosis of Bombyx mori L. Two storage proteins start to appear in haemolymph in early 5th instar stage and show the identical mobility with fat body proteins. These proteins show the high concentration in haemolymph in last instar stage but accumulate in fat body after pupation. Storage protein-2 shows the distinct pattern for general storage proteins in both male and females. This protein is involved with the formation of cuticle protein in late last instar stage and appears to be temperally deposited in midgut during the pupal stage. Also SP-2 shows the identity with vitellogenin electrophoretically and immunologically and especially the positive reaction with antibody against yolk protein during the pupal stage, demonstrating that the storage protein is closely related to the formation of yolk protein.

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