• Asian Journal of Turfgrass Science
• /
• v.12 no.3
• /
• pp.173-182
• /
• 1998

This study was conducted to evaluate the effect of plant growth inhibitors on "Penncross" creeping bentgrass in the green of golf course for increasing the summer quality. Chlormequat chloride (C) 250$\times$, 500$\times$, 1000$\times$, daminozide (D) 50$\times$, 100$\times$, 200$\times$, paclobutralzol (P) 1000$\times$, 2000$\times$, 4000$\times$, trinexpac-ethyl (T) 1000$\times$, 2000$\times$, 4000$\times$, uniconazole (U) 25$\times$, 50$\times$, 100$\times$ with three concentrations, respectively, were applied to creeping bentgrass. The leaf length, leaf width, and internode length of stolon were inhibited by all plant growth retardants applied as compared to that of control in which D-50, T-2000, and trinexpac-ethyl were the most effective among treatments on the inhibition of leaf length and internode length of stolen, respectively. Leaf thickness increased more in the treatments of C-250, D-50. T-2000 and T-4000 than any other treatments. On the other hand, shoot density which was represented by number of leaves per unit area was found to be the highest in P-2000 and P-4000 treatment. In this case, all plant growth retardants with no significant differences showed increase of 15-20% in density as compared to control. Total dry weight increment was higher in P-2000 and P-4000 while leaf dry weight increment was higher in T-4000. and U-25 and 50 than other treatments. According to the visual evaluation for the purpose of turf quality measurement, it was found that paclo-butralzol, trinexpac-ethyl and uniconazole were not suitable for green maintenance and extension because of making leaves to yellow. In conclusion, it was postulated that C-250, D-50, and T-4000 had possibility to use practically in the green area of golf course.lf course.

• The Korean Journal of Pesticide Science
• /
• v.13 no.4
• /
• pp.275-282
• /
• 2009

The effect of two plant growth-promoting rhizobacteria (PGPR) on plant growth and systemic protection against soft rot disease and stem rot disease of canola (Brassica napus), caused by Erwinia carotovora and Sclerotinia sclerotiorum was investigated in a laboratory and a greenhouse. Selected PGPR strains C4 and D8 were treated to canola seeds by soaking. Strains C4 and D8 significantly not only increased plant height and root length about 74% and 40.3% and also reduced disease severity of soft rot disease by 80% by C4 and D8 respectively, compared to the control. Especially strain C4 showed antifungal activity against 6 fungal pathogens, S. sclerotiorum, Rhizoctonia solani, Botrytis cinerea, Fusarium oxysporum, Phytophthora capsici and Colletotrichum acutatum. In greenhouse experiment, the seed treatment of both of them increased plant height, leaf width and leaf length of canola plant to 19.5% and 24.9%, 11.3% and 15.3%, and 14.1% and 20.7% by C4 and D8, respectively, and reduced disease severity of S. sclerotiorium. These results indicate that these two PGPR strains can decrease disease severity and increased plant growth under greenhouse condition. Therefore, these two bacteria have a potential in controlling Sclerotinia stem rot of canola. These strains have to investigate under field condition to determine their role of antibiosis, induced systemic resistance and plant growth promotion on canola.

• Korean Journal of Soil Science and Fertilizer
• /
• v.44 no.2
• /
• pp.200-205
• /
• 2011

This study was conducted to find the physico-chemical properties and the amino acid content of the pigs hoof hydrolysate, keratin protein and to investigate its fertilizer effect on the growth of crops. The keratin proteins such as pigs hoof were alkali-hydrolyzed to produce the hydrolysates. The chemical properties of the hydrolysate of pigs hoof was 6~7 of pH and $10{\sim}15dS\;m^{-1}$ of EC. Total amino acid contents released from the pigs hoof were 10.18%, respectively. The pot experiment was carried out for the cultivation of lettuce. The treatment design of these pot cultivation was composed of Control (compost + NPK), PHH-0.5, PHH-1.0, PHH-2.0 (${\times}2,000$ ; 1,000 ; 500 diluted solution of pig hoof hydrolysate). After lettuce cultivation, the pH values in all treatment soils were decreased than those in initial soils, and the exchangeable cation value was higher than that of control. In all PHH treatments, lettuce growth was better in the leaf length by 6~16% and the leaf width by 4~15% than in control. Therefore, the PHH solutions manufactured by hydrolysis process had plenty of amino acids, and among them PHH had the most abundant nutrients and amino acids with highest growth and yield effect on lettuce.

• Korean Journal of Soil Science and Fertilizer
• /
• v.43 no.4
• /
• pp.498-505
• /
• 2010

To evaluate the effectiveness of AMF on the growth of horticultural crops, we compared mycorrhizal and non-mycorrhizal plants, lettuce (Lactuca sativa L.), that were inoculated with AMF propagules. As compared to the AMF- seedlings, in AMF+ seedlings at 3 weeks after sowing, the number of leaves increased 9%, leaf fresh weight increased 59%, leaf area increased 58%, and leaf length and width increased 21-22%, and chlorophyll content increased 2%. Furthermore, at 9 weeks after sowing, compared to the AMF- plants, in lettuce plants inoculated with AMF at the sowing and transplanting stages, the number of leaves increased 21% and 18%, leaf fresh weight increased 51% and 41%, root fresh weight increased 56% and 47%, and chlorophyll content increased 18% and 19%, respectively. Further this experiment indicated that the growth responses of lettuce plants inoculated with AMF during transplanting were similar to those inoculated with AMF during sowing. The results imply that the AMF infection timepoint is not important. The P content in the leaves of lettuce plants inoculated with AMF during transplanting was significantly higher (217%) than that of leaves from lettuce plants not inoculated with AMF. In contrast, the P content of the leaves of lettuce plants inoculated with AMF during the sowing stage was similar to that of leaves of control lettuce plants. In this experiment, P and chlorophyll content in AMF+ lettuce plants were higher than in AMF- plants, indicating that the photosynthetic rate was improved with AMF inoculation.

• Korean Journal of Plant Resources
• /
• v.26 no.1
• /
• pp.84-89
• /
• 2013

We established that regenerated plants of Panax ginseng were directly transplanted into the soil without the acclimatization process. When regenerated plants were treated at the concentration of $0.4mg\;l^{-1}$ for 9 hr before transplantation into the soil, our results showed that the survival rate of them was the highest. The optimal size of the root was above 4 cm in length and 0.4 g by weight before transplantation into the soil. The highest survival rate increased to 59.6%. After 4 years of cultivation in the fields, our results showed that there were no significant morphological differences between regenerated plants and wild-type ginseng. Also, we obtained healthy seeds from regenerated plants. This study provides the basis for a relatively rapid multiplication of selected genotypes and will allow a much more rapid evaluation of the germplasm of the unexplored and unstudied Korean ginseng.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.10a
• /
• pp.49-49
• /
• 2018

본 연구는 관상가치가 높아 조경 및 관상소재로 개발이 가능한 큰개관중의 전엽체 증식 및 포자체 형성에 적합한 배양조건을 구명하고자 수행되었다. 실험재료는 무가온 온실에서 채집한 포자를 기내에서 발아시켜 전엽체를 획득한 후 8주 간격으로 계대배양하여 실험에 사용하였다. 배지종류에 따른 전엽체의 기내 증식 및 형태형성의 영향을 알아보고자 배양된 전엽체 300mg을 메스로 다진 다음 농도를 1/4, 1/2, 1, 2배로 조절한 MS와 Knop배지에 8주간 배양하였다. 그 결과, 1MS배지에서 전엽체의 생체중이 5.5g으로 가장 많이 증가하였다. 1MS를 제외한 타 처리구는 생체중의 증가수준이 1.1-3.0g에 머물러 1MS배지보다 저조한 수준을 보였다. 현미경을 이용한 전엽체의 관찰결과, 1MS배지는 엽육의 색이 녹색으로 생육이 양호하였다. 전엽체의 증식이 가장 저조하였던, 2MS와 1/4MS는 생육의 저조뿐만 아니라 노화현상도 관찰되었다. 포자체 형성을 위한 최적의 토양조건을 알아보고자, 원예상토, 피트모스, 펄라이트 및 마사토의 비율을 5종류로 달리하여 배양토를 혼합하였다. 혼합된 토양은 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진하여 기내배양된 전엽체 1g을 증류수와 함께 10초간 분쇄한 다음 토양표면에 분주 후 10주간 재배하였다. 그 결과, 원예상토가 높은 비율로 첨가된 혼합조건에서 포자체의 형성이 우수하였다. 그중 원예상토와 마사토를 2:1(v:v)로 혼합한 토양, 원예상토와 펄라이트를 2:1(v:v)로 혼합한 토양에서 포트 당 각 357.0, 339.8개의 포자체가 형성되었다. 또한 형성된 포자체의 생육을 조사한 결과 원예상토와 마사토를 2:1(v:v)로 혼합한 토양에서 생체중, 엽수, 엽장, 엽폭, 근수, 근장 및 SPAD value 등의 생육수치가 우수하였다. 따라서 큰개관중의 전엽체 증식에 적합한 배지는 1MS로 판단되었으며, 포자체 대량생산을 위해서는 원예상토와 마사토를 2:1(v:v)로 혼합한 토양이 적합하다고 판단된다.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.10a
• /
• pp.82-82
• /
• 2018

산채는 주로 산지에서 자생하는 것을 채취하거나 자생지와 비슷한 환경의 임간에서 재배하여 수확을 한다. 본 시험은 차광처리를 통해 산지가 아닌 노지에서 산채 재배 가능성을 검토하고자 하였다. 대상작목은 개미취(Aster tataricus), 눈개승마(Aruncus dioicus var. kamtschaticus), 단풍취(Ainsliaea acerifolia), 박쥐나물(Parasenecio auricudatus var. matsumurana), 산마늘(Allium microdictyon), 어수리(Heracleum moellendorffii), 우산나물(Syneilesis palmata)이다. 시험작목을 전북 남원시 허브시험장(표고 500m) 포장에서 2017년 4월에 종묘를 정식하여 약 2년간 재배하였다. 고온기인 5월부터 9월까지 35%, 55%, 75%, 95% 차광정도를 달리하여 설치 한 후 시설 내의 기온, 상대습도 등 환경조건 및 재배작물의 고사율, 출현율, 생육특성 등을 조사하였다. 시설 내부의 평균 기온은 차광정도가 높아질수록 다소 낮아졌으며, 상대습도는 점차 높아졌다. 차광정도별 고사율을 조사한 결과 개미취, 어수리, 우산나물의 경우는 차광비율에 상관없이 각각 3.6%, 5.7%, 9.8%로 낮게 나타났다. 반면 단풍취와 산마늘의 경우 차광 비율에 따른 편차가 크지는 않았지만 최대 고사율이 각각 20.0%, 36.8%로 다소 높게 나타났다. 또한 눈개승마 75% 차광에서 50.0%, 박쥐나물 35% 차광에서 100% 고사율을 보여 적정 차광비율 선정 필요하다고 판단된다. 정식 2년차 4월 평균 출현율은 개미취 100%, 눈개승마 86.2%, 단풍취 84.3%, 박쥐나물 77.9%, 산마늘 97.5%, 어수리 88.9%, 우산나물 96.3%이었다. 다만 단풍취, 박쥐나물은 차광정도가 높아질수록 출현율이 높아졌고, 어수리는 차광정도가 높아질수록 다소 출현율이 낮아졌다. 또한 생육특성 조사 결과 초장은 단풍취를 제외하고 차광처리별 경향성이 뚜렷하게 나타나지 않았으며, 엽장과 엽폭은 산마늘을 제외한 작목들은 차광이 강할수록 커지는 경향이었다. SPAD 값은 개미취와 산마늘은 처리구역별 차이가 없었으나, 나머지 작물은 차광이 높아질수록 SPAD 값도 커졌다. 시험결과 표고 500m지역에서 개미취, 산마늘, 어수리, 우산나물은 차광수준에 따른 생육의 차이가 크지 않거나 차광이 낮아도 생육이 비교적 양호하였다. 그러나 단풍취와 박쥐나물은 차광정도에 따라 생육의 차이가 분명하였으며, 차광이 높아질수록 생육이 좋아졌다. 이를 바탕으로 산채류 노지재배를 위해서는 연차 간 작목별 생육특성, 수량성, 상품율, 경제적 분석 등의 추가적인 조사가 이루어져 할 것으로 여겨진다.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.10a
• /
• pp.41-41
• /
• 2018

차꼬리고사리(Asplenium trichomanes L.)는 남방계식물로 제주도에 자생하는 것으로 알려져 있으며, 잎에 광택이 있고 총생하는 식물이다. 주로 실내 외 조경 및 분화소재로 이용되며, 한방에서는 철각봉미초라하여 뿌리를 포함한 전초를 이질, 임병, 만성질염, 월경불순 및 요통의 약재로 사용한다. 또한 식물구계학적 특정식물종 IV급, 적색자료목록 준위협 (NT)에 분류된 식물로 보호가 필요하다. 본 연구는 차꼬리고사리의 대량생산을 위한 기내전엽체 증식 및 기외포자체 형성조건을 구명하고자 수행되었다. 실험재료는 포자를 기내 발아시켜 전엽체를 획득한 다음 8주 간격으로 계대하면서 확보하였다. 전엽체의 증식과 생육에 적합한 배지를 비교하고자, 1/4, 1/2, 1, 2MS와 Knop배지를 조성하여 배양하였다. 배양은 전엽체 300mg을 메스로 균일하게 다지는 방법을 이용하였으며, 배양환경은 온도 $25{\pm}1.0^{\circ}C$, 광도 $30{\pm}1.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 광주기 16/8h(light/dark)로 조절되었다. 실험결과, 1MS배지에 배양된 전엽체의 생체중이 4.3g으로 가장 많이 증가하였다. 형태형성발달도 하트형의 전엽체로 정상적으로 유도되었으며, 생식기관도 관찰되었다. 전엽체로부터 포자체의 형성을 유도하고자, 원예상토, 피트모스, 펄라이트 및 마사토의 비율을 5종류로 달리하여 혼합된 토양을 사각분($7.5{\times}7.5{\times}7.5cm$)에 충진하였다. 전엽체 1g과 증류수 25mL를 핸드블랜더로 10초간 분쇄하여 토양표면에 분주하는 실험방법을 사용하였다. 재배환경은 온도 $25{\pm}1.0^{\circ}C$, 광도 $43{\pm}2.0{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, 광주기 16/8h(light/dark)로 유지하면서 12주간 재배되었다. 실험결과, 원예상토 단용, 원예상토와 펄라이트가 2:1(v:v)로 혼합된 토양, 원예상토와 마사토가 2:1(v:v)로 혼합된 토양에서 각 31.7, 24.3, 19.3개의 포자체가 생산되었다. 한편 포자체의 생육은 원예상토 단용 토양에서 엽수, 엽장, 엽폭 등의 수치가 비교적 우수하였다. 따라서 차꼬리고사리의 전엽체는 MS배지에 배양하고 증식된 전엽체를 원예상토에 분주하여 포자체의 형성을 유도하는 것이 가장 효과적이었다.

• Korean Journal of Medicinal Crop Science
• /
• v.3 no.2
• /
• pp.146-150
• /
• 1995

Weed control in the Korean ginseng(Panax ginseng C.A. Meyer) garden is more difficult than in other crops because the ginseng is a perennial crop and has to be cultivated under the artificial shading. As hand weeding is the only practical means to control weed after crop establishment, a selective herbicide would greatly simplify the control of weed in ginseng garden. In an exploratory experiment, the hervicide Fluazifop-butyl was found to be selectively safe for the Korean ginseng plants. Various rates of Fluazifop-butyl were sprayed on 2-, 3-, and 4-years old ginseng plants as a foliar spray to detect crop injury and to difine their critical concentration. No apparent injury to the ginseng plant was noticable even the doubled application rate of Fluazifop-butyl the based on recommended dosage. Neither abnormal foliar change nor any inhibition in leaf and stem growth was resulted for 2-, 3-, and 4-years old ginseng plants treated with Fluazifop-butyl tripled the recommended usage. The foliar treatments of Fluazifop-butyl did not influence the photosynthesis ability but inhibited the respiration of the ginseng leaf.

• Journal of Korean Society of Forest Science
• /
• v.103 no.4
• /
• pp.547-555
• /
• 2014

This study was carried out in order to investigate the germination and the early growth characteristics of Parasenecio firmus. Seed pre-treatment before the each experiment was carried out by shading treatment (with drying at room temperature (DRT), drying at low temperature (DLT) and water soaking (WS) for 48 hours). Experiment was performed by shading treatment (full sunlight, 35%, 50%, 75%, and 95% shading). Seeds of Parasenecio firmus were surveyed the highest germination rate (61.1%) in full sunlight with WS (overall 25.7~61.1%). Height was surveyed the highest under 95% shading. And root collar diameter was surveyed the highest in full sunlight. Fresh weights (leaf, shoot, root and total) were the highest under 50% shading. Dry weights (leaf, shoot and total) were the highest under 75% shading. It was indicated the lowest leaf, shoot, root and total under 95% shading. Leaf growth (leaf area, leaf length, leaf width and leaf thickness) and root growth (total root length, root project area, root surface area, root diameter and root volume) were good under 35%~75% shading, but the lowest under 95% shading. As a result of surveying the whole experiment, seed of Parasenecio firmus grows well under 50%~75% shading after germination by high sunlight with water soaking (WS).