• Korean Journal of Medicinal Crop Science
• /
• v.1 no.1
• /
• pp.43-48
• /
• 1993

Present experiments were carried out to examine the effect of plant growthregulators for callus induction and plantlet regeneration through leaf tissue culture of Scutellaria baicalensis GEORGI. The results indicated that Callus was induced well on MS medium supplemented with 0.5mg/L NAA or 0.5mg/L NAA Plus 0.5mg/L zeatin. MS medium supplemented with 1.0mg/L BAP plus 0. 5mg /L NAA or 1. 0mg /L zeatin Plus 0.5mg /L NAA and 1.0mg /L NAA were the most effective for plant regeneration. Thin layer chromatogram of baicalin component (Rf 0.39) was observed from callus cultured on MS medium containing 0.5mg /L NAA plus 0.5mg /L zeatin.

• Korean Journal of Plant Tissue Culture
• /
• v.26 no.1
• /
• pp.21-26
• /
• 1999

These experiments were attempted to introduce rolC gene in the Petunia hybrida cv. Titan white by Agrobacterium mediated. The maximum frequency of shoot regeneration was obtained by 60% on MS medium containing 1.0 mg/L BA, 0.1 mg/L NAA, 200 mg/L kanamycin, 500 mg/L carbenicillin, 30 g/L sucrose, and 8 g/L agar. Kanamycin-resistant calli were selected from petunia leaf discs by cocultivation with Agrobacterium suspension cultures on MS medium. The addition of AgNO$_3$ and KMnO$_4$ in the medium increased the shoot regeneration by 31.3% from leaf disc as compared with non-treated leaf disc. Among clones exhibiting kanamycin resistance, only 3 clones were confirmed by southern hybridization analysis.

• Journal of Plant Biology
• /
• v.34 no.1
• /
• pp.45-51
• /
• 1991

Morphological variability was quantified for Endarachne binghamiae J. Agardh from five local populations collected in December, 1989 and in March, 1990. The sampling sites were chosen at intervals of 50'N along the east coast of Korea. Fifty individuals were haphazardly collected for the relationship between the blade length and reproduction, among which twenty five individuals were randomly selected for morphological variation. The Korean plants of E. binghamiae had the capacity of reproduction at an early stage of growth. The holdfast diameter and the plurangial length showed clinal variation of increment from the north to the south whereas the cryptostomata from the south to the north. The holdfast diameter, the blade thickness, the number of plurangial blades and the cryptostomata increased from December to March, when the blade length decreased. Endarachne is distinguished by the densely interwoven hypha I cells in the medulla, [our to eighteen rows of the plurangial compartments, and absence of paraphyses between plurangia. Therefore E. binghamiae should not be combined into Petalonia, but rather the Soviet plants of P. zosterijolia may be renamed as a taxon belonging to the genus Endarachne.rachne.

• Korean Journal of Environmental Agriculture
• /
• v.22 no.1
• /
• pp.60-64
• /
• 2003

This study was conducted to examine the effects of plug tray cell size and growth media on good seedling production of Gerbera hybrida Hort. Seedlings were grown for 60 days in 50, 72, 128, 162 cell trays contanning perlite, cocopeat and perlite+cocopeat(1:1, v/v). Perlite showed higher bulk density than cocopeat and perlite+cocopeat. Total porosity was greater in perlite, cocopeat and perlite+cocopeat in order. Cocopeat showed the highest water holding capacity. Number of leaves were greatest in 128 cell tray containing cocopeat. Leaf area was greatest in 50 cell tray containing cocopeat. Seedling growth was also better in plug tray of bigger cell size. Seedling growth of fresh and dry weight of shoot and root was much better in the growth media of perlite+cocopeat.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.15 no.1
• /
• pp.13-18
• /
• 1995

Seedling development among reciprocal FI hybrids and their parents, Lotus comiculatus and L renuis, were studies using a simple method of measuring cotyledon area. Cotyledon area attached to the plant could be measured with simple rapid method. Cotyledons expanded very rapidly during the first week, then slightly expanded until maximum size was reached around 3 weeks after emergence. Cotyledon area upon emergence was correlated with seed size (P=0.05), but after emergence there was a divergence in cotyledon expansion rate. Rapidity of photosynthetic area development was determinant factor for seedling growth of trefoil species. 2 $\times$ m2 and 1 $\times$ m2 among the hybrids promised the possibility increasing seedling vigor of birdsfoot trefoil.

• Journal of Bio-Environment Control
• /
• v.11 no.1
• /
• pp.23-28
• /
• 2002

This experiment was conducted far the efficient use of plant growth retardants (PGR) for suppression of plug seedling height. 'Seokwang' tomato seeds were soaked in I5 mL solution of daminozide (1,000, 10,000, or 100,000 mg.L-1) or uniconazole (1, 10, or 100 mg.L-1). And then, they were put in $25^{\circ}C$ chamber for one or three days. Seeds were washed in tap waters and were dried in 5$^{\circ}C$ chamber for one day. Dried tomato seeds were sown and seedlings were raised in 288-cell plug trays. Seedlings grown were evaluated at 21 and 38 days after sowing. Treatments of 10 or 100 mg.L-1 uniconazole as compared to the control had significantly suppressed hypocotyl length and seedling height, but fresh and dry weights of roots, emergence, no. of leaves, chlorophyll concentration, dry matter, and T/R ratio were not significant by different among treatments.

• Journal of Plant Biotechnology
• /
• v.31 no.3
• /
• pp.249-253
• /
• 2004

The type of air lift bioreactor affected the root growth in ginseng adventitious root cultures. Among bioreactors used in this experiment, bulb type bubble bioreactor (BU) was the best to increase root growth (41.92 g dry weight). The kLa value representing the oxygen transfer capacity from medium to explants (6.98 h$^{-1}$ ) in BU with 5 cm bubble column was higher than other bioreactors. On the other hand, cylindric tube bioreactor (CT) without bubble column resulted in minimum root growth (38.55 g dry weight) and kLa value (5.25 h$^{-1}$ ). Furthermore, the root growth (50.30 g dry weight) in BU with 10 cm bubble column more increased than 5 cm bubble column. However, the kLa value do not affected the secondary metabolite such as ginsenosides. These results show that the bubble column in air lift bioreactor increase kLa value and increased kLa value stimulate the growth of ginseng adventitious roots.

• Journal of Bio-Environment Control
• /
• v.16 no.3
• /
• pp.205-209
• /
• 2007

This experiment was conducted to investigate the growth of plug seedlings and yield of tomato affected by the concentration of triazole and the treatment time. The length and leaf area of tomato seed-lings were reduced by the application of triazole compared to control. Growth reduction of seedlings was greater by earlier application and higher concentration of triazole. The growth of tomato treated with hexaconazole were similar with control 4 weeks after sowing. The triazole treatment lowered the height of the first fruit set compared to control. However, triazole treatment did not affect the number of fruits and yield.

• Korean Journal of Medicinal Crop Science
• /
• v.6 no.1
• /
• pp.62-69
• /
• 1998

There was no significant difference in length and width of leaf and number of leaves per plant between tissue-cultured plants and conventionally propagated ones but chlorophyll content increased in tissue-cultured ones. Percent of sprouting from planted root segments significantly increased in tissue-cultured plants, resulting in yield increase of more than 200% per 10a. Root thickness of tissue-cultured plants at the time of planting influenced percent of sprouting and yield. Plants with root diameter ranging from 3 to 6mm gave good yield. When virus infection was monitored with N. tabacum and C. amaranticolor as indicator plants, 100% infection occurred in vegetatively propagated plants and introduced plants from China. whereas plants obtained from apical meristem showed 0% and 40% to 45% infection in vitro plantlets and 1 year old plants in vivo, respectively. Tobamovirus and unidentified virus particles were detected in electron microscopy.

• Journal of Korean Society of Forest Science
• /
• v.67 no.1
• /
• pp.10-16
• /
• 1984

Containerized Quercus acutissima seedlings were inoculated with vegetative mycelial inoculum of Pisolithus tinctorius and were grown in a green-house. The ectomycorrhizal fungus, P. tinctorius (Pt) increased growth of the container-grown oak seedlings. Of three treatments, Pt inoculation stimulated remarkably primary lateral roots, shoot growth, and root collar diameter and leaf area development, compared with either non-inoculated or natural soil treatment. In addition, Pt-inoculated seedlings produced ectomycorrhizae, by 71.1 percent. However, noninoculated and naturally grown seedlings were free of natural ectomycorrhizal. Ectomycorthizal roots developed more abundantly in the middle portion of the container than in the top or bottom portions.