• Korean Journal of Poultry Science
• /
• v.42 no.2
• /
• pp.109-116
• /
• 2015

Ogye rooster sperm chromatin status can be detected using well established sperm assays. In this paper, a simple and fast method to monitor rooster sperm chromatin status could be employed in field for assessment of chicken sperm quality. Using standard bright field microscope, Diff-Quik stains can be reproducibly, easily and routinely monitored with simple staining. The presence of abnormal chromatin staining of rooster sperm was determined by darker stain in head. In the fresh semen, the viabilities of three tested Ogye spermatozoa were 93.53%, 82.42% and 90.63% and normal chromatin rates were 87.96%, 74.25% and 85.10% respectively. However, after freezing, the rates of viability of thawed semen were reduced to 69.58%, 61.98% and 72.20% and normal chromatin rate also reduced to 58.91%, 48.49% and 63.34%. A significant correlation between live sperm and normal sperm nuclei was 0.875 in fresh semen and 0.513 in frozen semen. After incubation of sperm at $37^{\circ}C$ for 5min, the rates of viability, chromatin normality and sperm head activity were shown as $90.63{\pm}1.28%$, $82.44{\pm}8.09%$ and $66.68{\pm}10.29%$ in fresh semen. However, the rates of thawed semen were reduced to $67.92{\pm}7.55%$, $56.92{\pm}12.15%$ and 47.32{\pm}5.02%, respectively. The relationship between chromatin normality and sperm head movements in fresh and thawed semen were 0.564 and 0.540, respectively. With these results, the chicken sperm normality could be assessed by the Diff-Quik staining that could be used for chromatin status of sperm head and activated morphology of live spermatozoa, as a simple and rapid staining method.

• Clinical and Experimental Pediatrics
• /
• v.46 no.6
• /
• pp.536-540
• /
• 2003

Purpose : Aspiration of foreign material into the lungs can cause acute or chronic pulmonary diseases. It is difficult to detect small amounts of aspiration due to the lack of safe, sensitive and specific diagnostic tests. Recently, in animal or human studies, it has been reported that immunochemistry for lactalbumin can be used to detect the minimal aspiration. So, the authors' investigation was designed to determine whether human milk phagocytized alveolar macrophages can be detected in human milk aspirated mice. Methods : Sixty four male mice, 6-8 weeks old and 30-40 gm weighing, were used for this study. About 0.05 mL of human milk or normal saline were given intranasally once per day for 1 day or 3 days. Under anesthesia with ketamine and xylazine, the trachea of each mouse was cannulated with an 18G Jelco needle and then, each mouse's lungs were lavaged three times with 0.5 mL of phosphate buffer solution at 2, 8, 24, and 48 hours after the last milk or normal saline instillation. Cells in bronchoalveolar lavage fluid were stained with Oil Red O and immunocytochemistry for alpha-lactalbumin. Results : Immunocytochemical reactivity for alpha-lactalbumin or lipid-laden alveolar macrophages were not observed in the normal saline aspirated groups. Immunocytochemical reactivity for alpha-lactalbumin were observed in the human milk aspirated groups. They showed a peak at 8 hours and decreased markedly at 24 hours but persisted even at 48 hours after aspiration. Immunocytochemical stain positive alveolar macrophages were noted similarly in number between single and multiple aspiration groups. Conclusion : These observations suggested that alveolar macrophages for lactalbumin could be more easily detected on immunocytochemistry than Oil Red O stain, and immunocytochemistry could be used as a sensitive and specific diagnostic test for the detection of human milk aspiration.

• Proceedings of the Korean Information Science Society Conference
• /
• 1998.10a
• /
• pp.750-752
• /
• 1998

본 논문은 고품질의 정보를 신속하게 제공할 수 있으며, cost-effective 한 medium-grained 병렬 정보 검색 시스템을 제시하고 있다. 본 검색 시스템은 병렬 모델의 효율을 극대화하는 방안으로 문서 라이브러리를 작은 단위의 클러스터로 세분화하고 검색 시 클러스터 단위로 프로세서에 할당될 수 있게 하여 할당될 작업의 단위를 적절히 중규모화하였을 뿐만 아니라, 각 클러스터마다 독립적인 염색인 파일을 별도로 두어 순위 부여 계산시 통신을 최소화 할 수 있도록 하였다. 또한, 기계 학습 기법을 이용하여 가능한 한 유사한 문서군이 되도록 클러스터링 함으로써 불필요한 크러스터가 검색될 가능성을 최소화하여 성능을 높였다. 본 검색 시스템은 분산메모리 MIMD 구조의 트랜스퓨터에서 구현되었으며, Connection machine에서 사용되는 Stanfill방법과의 비교 실험을 통하여 계층적인 접근법의 성능을 비교, 평가하였다. 그리고 random클러스터링 기법과 비교하여 기계학습을 통한 클러스터링 접근방법이 우수함을 보이고 있다.

• Journal of the Korea Computer Graphics Society
• /
• v.24 no.1
• /
• pp.9-16
• /
• 2018

In this paper, we introduce a visualization framework for cell image data obtained from optical diffraction tomography (ODT), including a method for representing cell morphology in 3D virtual environment and a color mapping protocol. Unlike commonly known volume data sets, such as CT images of human organ or industrial machinery, that have solid structural information, the cell image data have rather vague information with much morphological variations on the boundaries. Therefore, it is difficult to come up with consistent representation of cell structure for visualization results. To obtain desired visual representation of cellular structures, we propose an interactive visualization technique for the ODT data. In visualization of 3D shape of the cell, we adopt a volume rendering technique which is generally applied to volume data visualization and improve the quality of volume rendering result by using empty space jittering method. Furthermore, we provide a layer-based independent rendering method for multiple transfer functions to represent two or more cellular structures in unified render window. In the experiment, we examined effectiveness of proposed method by visualizing various type of the cell obtained from the microscope which can capture ODT image and fluorescence image together.

• Tuberculosis and Respiratory Diseases
• /
• v.56 no.3
• /
• pp.261-268
• /
• 2004

Background : The resurgence of tuberculosis and outbreaks of multidrug resistant (MDR) tuberculosis have increased the emphasis for the development of new susceptibility testing of the Mycobacterium tuberculosis for the effective treatment and control of the disease. Conventional drug susceptibility testings, such as those using egg-based or agar-based media have some limits, such as the time required and difficulties in determining critical inhibitory concentrations, but these are still being used in many diagnostic laboratories because of no better lternatives, considering cost and accuracy. To overcome these limits, a rapid and simple method for new susceptibility testing, using live and dead assays, was applied for a bacterial cell viability assay to distinguish dead from live bacterial cells based on two-color fluorescence. Materials and Methods Strains : Forty strains were used in this study, 20 susceptible to all antituberculosis drugs and the other 20 resistant to the four first line antituberculosis drugs isoniazid, rifampicin, streptomycin and ethambutol. Antibiotics : The four antibiotics were dissolved in 7H9 broth to make the following solutions: $0.1{\mu}g\;isoniazid(INH)/m{\ell}$, $0.4{\mu}g\;rifampicin(RMP)/m{\ell}$, $4.0{\mu}g\;streptomycin(SM)/m{\ell}$ and $4.0{\mu}g\;ethambutol(EMB)/m{\ell}$. Results : Live and dead Mycobacterium tuberculosis cells fluoresced green and red with the acridin (Syto 9) and propidium treatments, respectively. These results are very well accorded with conventional drug susceptibility testing by proportional method on Lowensen-Jensen media (L-J) containing 4 drugs (INH, RMP, EMB and SM), showing a 93.7 % accordance rate in susceptible strains and 95% in resistant strains. Conclusion : The results of the drug susceptibility testing using the live and dead bacterial cell assay showed high accordance rates compared with the conventional proportion method on L-J. This finding suggests that the live and dead bacterial cell assay can be used as an alternative to conventional drug susceptibility testing for M. tuberculosis strains.

• Textile Coloration and Finishing
• /
• v.28 no.3
• /
• pp.189-194
• /
• 2016

The objectives of this research were to study the effect of salt-shrinkage finishing of silk on shrinkage degree and dyeing property of cochineal, and to investigate the efficacy of obtained results for imparting surface design effect on silk fabric. Ultimately it was aimed to suggest a technical process for developing natural dyed silk products with diverse design. Premordanted silk fabric was treated with $Ca(NO_3)_2$ solution(gravity: 1.45) at $90^{\circ}C$ for 1 min, washed, dried for further evaluation. The shrinkage of salt-treated fabric was calculated. The effects of salt treatment on the dye uptake of cochineal and colorfastness were investigated. The degrees of shrinkage were 10% and 3% for warp and weft, respectively. The salt treatment resulted in improving dye uptake of cochineal slightly. In addition, it improved colorfastness to washing and light. On the basis of the results, a technical process composed of premordanting, salt treatment and natural dyeing was suggested and using the process, two examples of textile design were presented. It can be concluded to impart various three dimensional surface design effect on silk fabric by applying salt-shrinkage finishing with combination of natural dyeing and mordanting.

• The Journal of the Convergence on Culture Technology
• /
• v.6 no.2
• /
• pp.117-122
• /
• 2020

In this study, among the new industries that are attracting attention as a new industry to lead the 4th Industrial Revolution era, Korean traditional culture and arts are being promoted in the context of local community efforts and collaboration between industry and academia. It aims to create jobs through local traditional culture, arts, tourism events and design product development, while seeking ways to secure labor force that can save and revitalize high value-added industries in the community. In particular, in the current situation where the era of change and the education of traditional techniques are increasingly ignored and neglected, this study is a traditional cultural art through natural dyeing education and cultural and art events conducted by industry, regional universities, and research institutes in Pyeongtaek for many years. It is expected to be used as a basic data for the transmission and creation of high value-added jobs.

• Journal of Acupuncture Research
• /
• v.17 no.2
• /
• pp.261-276
• /
• 2000

본 실험은 pseudorabies 바이러스 (PRV) 의 Bartha strain 을 안면신경의 측두지, 하지를 지배하는 신경 (좌골신경) 및 상지를 지배하는 신경 (요골, 척골, 정중신경) 에 주입한 후 4 일간의 생존시간이 경과한 후 척수와 뇌를 적출하여 동결절편을 제작한 후 면역조직화학적 염색기법과 X-gal 조직화학 염색법을 시행하여 염색된 신경세포체를 척수와 뇌에 투사된 공통영역을 관찰하고 두침의 영역중 하나인 운동구와 사지와의 관계에 대한 실험적 증거를 제시하고자 시행하였다. 위의 실험에서 얻어진 결과는 아래와 같다. 1. 안면신경의 측두지, 하지를 지배하는 신경 (좌골신경) 및 상지를 지배하는 신경 (요골, 측골, 정중신경) 에서 투사된 공통된 영역은 척수에서 경수의 층판 1-IV, 흉수의 intermediolateral nucleus(IML), dorsal nucleus(D) 및 층판 X, 요수의 층판 IV, V, 천수의 층판 IV, V, IX, X 등의 영역에서 관찰되었고, 뇌줄기에서는 caudoventrolateral reticular nucleus(CVL), nucleus solitary tract(Sol), rostroventrolateral nucleus(RVL), area postrema(AP), raphe nuclei(raphe pallidus, raphe obscurus, raphe magnus), inferior olivary nucleus 의 등쪽부분 (gigantocellular reticular nucleus, Gi), Kolliker-Fuse nucleus(KF), central gray(CG), dorsal raphe nucleus (DR) and A5 영역에 표지된다. 또한 paraventricular hypothalamic nucleus(PRV) 와 lateral hypothalamic reticular nucleus(LH)에서도 관찰되고 locus coeruleus(LC) 와 subcoeruleus nuc!eus(SubCA) 에서도 관찰된다.

• Journal of Korean Society of Forest Science
• /
• v.80 no.2
• /
• pp.232-236
• /
• 1991

The efficacy of berberine sulphate, a fluorochrome having binding properties with both DNA and RNA, was investigated for the detection of mycoplasma-like organisms(MLOs) in jujube(Zizyphus jujuba), paulownia(Paulownia tomentosa), mulberry(Morus alba) trees and periwinkle (Catharanthus roseus) plant. When examined under fluorescence microscope, berberine sulphate-stained sections of diseased samples showed distinct MLO-specific fluorescent particles in the phloem area, while such fluorescence was absent in the healthy ones. This staining technique was proved to be a very accurate method for the diagnosis of MLO infections in woody and herbaceous plants. Furthermore, the cheap and easy procedure could be used to test a great number of samples on MLO infections with reliability and rapidity.

• Proceedings of the Korean Society of Dyers and Finishers Conference
• /
• 2012.03a
• /
• pp.102-102
• /
• 2012

수분산 폴리우레탄 수지는 기능성이 뛰어나고 다양한 용도로 쓰임이 많기 때문에, 용제형 폴리우레탄 수지를 이용한 환경문제에 대처할 수 있는 대안으로 그 쓰임이 점차 늘어나고 있다. 그러나 수분산 폴리우레탄 수지는 용제형 폴리우레탄 수지에 비해 물리적 특성이 떨어지는 단점이 있다. 그래서 우수한 물리적 특성을 유지하면서 용제형 폴리우레탄 수지를 대체할 수 있는 환경친화성 폴리우레탄 수지에 대한 연구가 많이 이뤄지고 있다. 최근 산업환경 변화에 순응하는 환경 친화적 소재를 사용하여 자동차, 선박, 철도 등 수송용 인테리어 내장재를 구성하는 기술로서, 난연성 및 기계적 물성 유지 기술, 그리고 실내 쾌적감을 부여하기 위한 감성기술 및 심미적 요소를 부여하는 디자인 기술이 가미된 복합 기능화 기술개발이 요구되고 있다. 기존의 자동차용 시트 제품의 대부분은 습식 PU와 PVC를 사용하고 있고, 제조과정에서 용제와 가소제 사용 등으로 인해 환경적으로 많은 문제점이 있으며, 이를 대체하기 위한 노력이 많은 부분에서 이루어지고 있지만, 마땅히 대체할 소재를 찾지 못하고 있는 실정이다. 본 연구에서 제안하는 Frothing 기법을 이용한 친환경 foam 제조기술은 기존의 습식 PU foam과 PVC foam을 대체할 수 있는 기술로서, PUD foam의 기계적 물성 및 가공성이 보완된다면 상업적 용도로 많은 분야에서 활용될 것으로 판단된다.