• The Korean Journal of Malacology
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• v.12 no.2
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• pp.133-140
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• 1996

한국산 다판류 4종의 정소를 재료로 warm drying method를 이용하여 핵형 분석을 실시하였다. 연두군부과 (Ischnochitonidae)에 속하는 북방줄군부(Lepidezona albrechtii)의 염색체, 그리고 한쌍의 차중부염색체로 구성되어 있으며,줄군부(Lepidozona coreanica)의 염색체는 2n=24, 중부 염색체 8쌍, 중부 또는 차중부염색체로 구성되어 있었으며, 크기로는 별도의 염색체군으로 나누어지지 않았다. 따가리과(Mopaliidae)에 속하는 따가리(Placiphorella stimpsoni)의 염색체는 2n=24, n=12, 핵형분석결과 6쌍의 중부염색체, 한쌍의 차단부, 그리고 5쌍의 말단부염색체였으며, 1변과 2번 염색체쌍은 다른 염색체쌍들 보다 월등히 큰 것으로 나타났다. 군부과 (Chitonidae)에 속하는 꼬마군부(Chiton kurodai)의 염색체는 2n=24, n=12,중부염색체 7쌍, 차중부 4쌍, 그리고 한쌍의 차단부염색체였고. 1번 염색체쌍이 크기에 의해 다른 염색체쌍들과 구분되었다. 본 연구의 결과와 과거의 연구 결과를 종합하면, 연두군부 과는 2n=24;따가리과는 2n=12및 24; 군부과는 2n=24및 26의 염색체를 갖고 있는 것으로 요약되며, 군부속 (Ginus Chiton)에서는 속내 종간 숫적 변이가 나타남이 확인되었다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2002.11b
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• pp.36-37
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• 2002

율무(Coix lacryma-jobi)의 핵형 분석을 한 결과 1번 염색체는 short arm과 long arm의 크기가 같은 metacentric chromosome이었으며, 전체의 염색체에 대한 상대적인 길이가 12.8로 가장 길었다. 1번 염색체는 단완과 장완의 양쪽 터미널에 각각 하나씩의 밴드를 나타냈으며 단완과 장완 각각에 interstitial band가 나타났다. 또한 1번 염색체는 단완 끝에 부수체를 갖는 NOR 염색체임을 알 수 있었다. 2번 염색체는 Short arm에 비해 long arm이 길었으며, 전체의 염색체에 대한 상대적인 길이는 11.8로 1번 염색체 다음으로 길었다. 2번 염색체 단완 끝에 하나의 터미널 밴드와 양완의 중간에 각각 interstitial 밴드를 나타냈다. 3번 염색체는 2번 염색체 보다 short arm의 길이보다 더 짧았으며, 상대적인 길이는 10.8로 2번 염색체보다 짧았다. 3번 염색체는 단완 끝에 하나의 terminal 밴드와 centromere 밴드를 나타냈다.(중략)

• The Journal of the Korea Contents Association
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• v.5 no.3
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• pp.29-35
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• 2005

The task for chromosome analysis and diagnosis by experienced cytogenetists are being concerned as repetitive, time consuming job and expensive. For that reason, intelligent agent based on chromosome knowledge base using web has been developed to be able to analyze chromosomes and obtain necessary advises from the knowledge base instead of human experts. That is to say, the knowledge base of IF THEN production rule was implemented to a knowledge domain with normal and abnormal chromosomes, and then the inference results by the knowledge base could enter the inference data into the database. Experimental data were composed of normal chromosomes of 2,736 cases and abnormal chromosomes of 259 cases that have been obtained from GTG-banding metaphase peripheral blood and amniotic fluid samples. The completed intelligent agent for the chromosome knowledge base provides variously morphological information by analysis of normal or abnormal chromosomes also has the advantage of being able to consult with the user on the chromosome analysis and diagnosis.

• Korean Journal of Ichthyology
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• v.2 no.2
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• pp.211-216
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• 1990

Mitotic analyses of the coho salmon (Oncorhynchus kitsuch) revealed a mode of 2n=60 whit 46 meta or submetacentric and 14 acrocentric chromosomes (NF=106). Individuals with 2n=61 were also found in both sexes in the same population; the karyotype is composed of 45 meta or submetacentric and 16 acrocentric chromosomes (NF=106). This result may indicate that Robertsonian chromosomal polymorphism occurred in the population of the coho salmon.

• Korean Journal of Medicinal Crop Science
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• v.10 no.2
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• pp.144-146
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• 2002

Cytogenetic analysis of Anemarrhena ashodeloides BUNGE, which is one of medicinal plants belonging to Haemodoraceae was carried out using Feulgen staining. The somatic metaphase chromosome number was identified to 2n=22 (x=11) and the size of chromosomes ranges from $1.27-3.80\;{\mu}m$. Three pairs of chromosomes were relatively long in total length and the others were short. The karyotype was bimodal in chromosome length and arm ratios. The chromosome complement comprise eight pairs of metacentric (chromosome 2, 3, 6, 7, 8, 9, 10, and 11), two pairs of submetacentric (chromosome 4 and 5), and one pair of subtelocentric (chromosome 1).

• Journal of Genetic Medicine
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• v.7 no.2
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• pp.111-118
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• 2010

Chromosomal microarray analysis (CMA) enables the genome-wide detection of submicroscopic chromosomal imbalances with greater precision and accuracy. In most other countries, CMA is now a commonly used clinical diagnostic test, replacing conventional cytogenetics or targeted detection such as FISH or PCR-based methods. Recently, some consensus statements have proposed utilization of CMA as a first-line test in patients with multiple congenital anomalies not specific to a well-delineated genetic syndrome, developmental delay/intellectual disability, or autism spectrum disorders. CMA can be used as an adjunct to conventional cytogenetics to identify chromosomal abnormalities observed in G-banding analysis in constitutional or acquired cases, leading to a more accurate and comprehensive assessment of chromosomal aberrations. Although CMA has distinct advantages, there are several limitations, including its inability to detect balanced chromosomal rearrangements and low-level mosaicism, its interpretation of copy number variants of uncertain clinical significance, and significantly higher costs. For these reasons, CMA is not currently a replacement for conventional cytogenetics in prenatal diagnosis. In clinical applications of CMA, knowledge and experience based on genetics and cytogenetics are required for data analysis and interpretation, and appropriate follow-up with genetic counseling is recommended.

• Journal of Aquaculture
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• v.3 no.2
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• pp.145-153
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• 1990

Genetic analysis of rainbow trout populations in hatchery stocks is important in order to develop a strategy for their management. In this study, chromosome number and polymorphisms of diploid and artificially induced triploid rainbow trout are discribed. The relation-ship between chromosomal polymorphism and their economic values for the aquaculture industry are also discussed.

• Proceedings of the Korean Information Science Society Conference
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• 2010.06c
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• pp.226-229
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• 2010

본 연구에서는 다양한 density profile 특징추출에 기반한 염색체 자동분류방법들의 성능을 비교분석하였다. density profile은 염색체의 밴드패턴을 가장 잘 표현한 특징으로 염색체의 중심축을 구성하는 화소들의 밝기 값을 추출하는 방법이다. 염색체의 밴드패턴은 염색체의 끝단까지를 잘 표현해주어야만 정확한 염색체번호를 확인할 수 있다. 따라서 염색체의 중심축을 추출하여 염색체 끝단까지 확장 처리한 방법에 대한 성능을 확인하였다. 염색체 중심축에 위치한 화소만을 이용한 프로파일은 잡음에 민감할 수 있으므로 이를 해결하기 위하여 염색체의 중심축에 대한 화소 값 대신 주변 밝기 값들에 대한 평균을 이용한 국소평균방법과 중심축의 수직라인 상에 존재하는 화소 값들에 대한 평균을 구한 수직평균방법을 비교하였다. 분류알고리즘은 k-NN을 사용하였고, 실험데이터는 (주)Gendix 로부터 제공받은 임상적으로 정상인 100명(남자 50명, 여자 50명)으로부터 추출한 4600개의 염색체 영상을 훈련데이터와 테스트데이터로 각각 50%씩 랜덤하게 분리하여 실험하였다. 실험결과 중심축을 확장하고 수직평균에 대한 프로파일을 특징으로 추출하여 분류한 경우가 가장 좋은 성능을 보였다.

• Korean Journal of Poultry Science
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• v.13 no.2
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• pp.167-172
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• 1986

Chromosome size, number and shape were studied by the centromeric index, the arm ratio and the relative length of chromosome. The chromosomes of 50 early chick embryos which were derived from a pure line of Single Comb White Leghorns were examined. Using a colchicine, hypotonic treatment, fixation and air-drying technique, the clear prometaphase figures were obtained from the whole embryo. The results of the present investigation of chromosome pairs were as follows, 1. Pair 1 and 2; metacentric and submetacentric chromosomes which could be clearly distinguished from each other by size. 2. Pair 3 and 4: acrocentric chromosomes of similar length but the 4th pair had a distinct short arm which was not present in the 3rd. 3, Pair 5; metacentric sex chromosomes, 2 chromosome had relative 5th length but the W chromosome had slightly shorter length than 7th pair of chromosomes. 4. Pair 6; acrocentric chromosomes similar in shape to pair 3 but of little more than half the size. 5, Pair 7 and 8; acrocentric chrocentric but the 7th pairs had a definite short arm. 6. Pair 9; similar length to the 7, 8 pairs but had a medially placed centromere. 7. microchromosomes of 30 pairs ; nearly all acrocentric chromosomes which appeared as paired dots. The total number of diploid was appeared to 72-78. But a number of observations presented the total diploid number in 78 (58%). The inconstancy in number observed in this study was presumably due to the minute size of the microchromosomes. Thus, the modal numbers for the diploid chromosome was at least 78.

• The Korean Journal of Zoology
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• v.31 no.4
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• pp.309-317
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• 1988

내륙산 관박쥐와 제주산 관박쥐간의 동-종 여부를 조사하기 위하여 경남과 제주에서 채집한 이들 관박쥐들을 핵형분석하였다. 핵형분석의 결과, 2종 모두 염색체수는 2n=58이었으며 FN도 62였다. 상염색체에서는 중형의 2쌍과 미세형 1쌍이 중부염색체(metacentrics)였고, 대형에서 소형까지는 25쌍이 단부염색체facrocentrics)이었다. 성염색체에서 X염색체는 대형의 차중부염색체(submetacentrics)였고, Y염색체는 소형의 단부염색체(acrocentrics)이었다. 그리고 이들 염색체들은 동원체 부근에 이질염색체을 가지는 특이한 1쌍의 단부염색체(acrocentrics)가 존재하고 있었다. in this study, We analysed the karyotypes of the inland bat(Rhinolox)thus femequinum hora) and the Cheju-Island bat(Rhinorophus ferrumequinum quelpartis(\ulcorner)) collected in Kyungnam and Cheju provinces to identify the homogeneous between them. The results are as follows. The diploid number of chromosomes of them are equally 58 and the fundamental number 62. In the autosomes, metacentrics consist of two pairs of the middle form and a pair of the micro-form. And acrocentrics have 25 pairs of large and small form. In sex-chromosomes, X-chromosome is a large submetacentrics and Y is a small acrocentrics. And, these chromosomes possess a pair of particular acrocentrics having heterochromatin around centromere in both the inland bat(Rhinolophus fenmequinum korai ) and Cheju-Island bat(Rhinolophus fewmequinum querporis (\ulcorner)).