• Journal of the Korean Institute of Intelligent Systems
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• v.16 no.2
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• pp.131-137
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• 2006

In swarm robot systems, each robot must behaves by itself according to the its states and environments, and if necessary, must cooperates with other robots in order to carry out a given task. Therefore it is essential that each robot has both learning and evolution ability to adapt the dynamic environments. In this paper, the new learning and evolution method based on reinforcement learning having delayed reward ability and distributed genetic algorithms is proposed for behavior learning and evolution of collective autonomous mobile robots. Reinforcement learning having delayed reward is still useful even though when there is no immediate reward. And by distributed genetic algorithm exchanging the chromosome acquired under different environments by communication each robot can improve its behavior ability. Specially, in order to improve the performance of evolution, selective crossover using the characteristic of reinforcement learning is adopted in this paper. we verify the effectiveness of the proposed method by applying it to cooperative search problem.

• Journal of the Korean Institute of Intelligent Systems
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• v.17 no.5
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• pp.591-597
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• 2007

In swarm robot systems, each robot must behaves by itself according to the its states and environments, and if necessary, must cooperates with other robots in order to carry out a given task. Therefore it is essential that each robot has both learning and evolution ability to adapt the dynamic environments. In this paper, the new polygon based Q-learning algorithm and distributed genetic algorithms are proposed for behavior learning and evolution of collective autonomous mobile robots. And by distributed genetic algorithm exchanging the chromosome acquired under different environments by communication each robot can improve its behavior ability Specially, in order to improve the performance of evolution, selective crossover using the characteristic of reinforcement learning is adopted in this paper. we verify the effectiveness of the proposed method by applying it to cooperative search problem.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.9
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• pp.1045-1052
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• 2017

In this study, a battery of genetic-toxicity studies on corn silk extract with high maysin content were performed according to internationally accepted protocols. In a mutation test using Salmonella Typhimurium TA1535, TA1537, TA98, and TA100, the number of mutant colonies did not significantly increase up to a maximum concentration of $5,000{\mu}g/plate$ in the presence or absence of the S9 metabolic activation system. In the chromosome aberration test using Chinese hamster lung fibroblasts, negative results were observed in the concentration up to $1,250{\mu}g/mL$ of corn silk extract. In the micronucleus test using ICR mice, incidence of polymorphonuclear erythrocytes with a maximum concentration of 2,000 mg/kg corn silk extract did not show any significant difference compared to the negative control group. Based on these results, the test substance, con silk extract, did not influence genotoxicity.

• Journal of Food Hygiene and Safety
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• v.33 no.3
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• pp.207-213
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• 2018

This study aimed to evaluate the genotoxicity of Litsea japonica fruit-hexane extract (LJF-HE). In order to examine the genotoxicity, we carried out bacterial reverse mutation assay, chromosome aberration assay, and a micronucleus induction (MN) test according to the OECD and the Korea Ministry of Food and Drug Safety (MFDS) toxicity test guidelines. In the bacterial reverse mutation assay, no significant increase in revertant colonies, nor bacterial toxicity, was observed in the LJF-HE treatment group, regardless of the absence or presence of metabolic activation by the S9 mixture. However, in the positive control group, revertant colony counts were shown to be more than twice that of the negative control group. The chromosome aberration test showed that the repetition rate of abnormal chromosome aberration was less than 5%, regardless of the treatment time, and with or without the S9 mixture. No significant change was observed when (p < 0.05) compared with the negative control group. The micronucleated polychromatic erythrocytes (MNPCE) repetition rate of the polychromatic erythrocytes (PCE) showed no significant changes when compared with the negative control group (p < 0.05). The PCE portion of total erythrocytes also showed no significant changes (p < 0.05). These results showed that LJF-HE had no significant genotoxic effects.

• Korean Journal of Poultry Science
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• v.17 no.1
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• pp.1-6
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• 1990

For the production of tetraploid embryos in chicken through induction of diploid gametes. The experiment was found results as follows; 1. Induction of diploid sperm was observed 11% from embryos of normal females and males at 12 days after injection of 0.37mg per 2kg body weight for three days. 2. 3 among 109 embryos from crosses of diploid gametes induced by TEM were found as tetraploid. 3. Genetic structures of tetraploid embryos were indentified as normal 4 pairs chromosomes without certain variation.

• Proceedings of the Korean Institute of Intelligent Systems Conference
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• 1998.03a
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• pp.16-19
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• 1998

본 논문에서는 생물학적인 DNA와 인위적인 DNA의 개발 메커니즘에 근거를 둔 새로운 DNA coding mithod를 소개한다. 이 방법은 DNA 유전자의 Redundancy 성질과 Over-lapping 성질을 분석하여 구성한 알고리즘으로, DNA 자체의 특성 때문에 염색체의 길이를 자유자재로 변화시킬 수 있는 장점을 가지고 있다. 즉 삽입과 삭제의 과정을 쉽게 처리할 수 있다. 따라서 본 연구에서는 이 DNA coding method를 사용하여 시스템 제어에 필요한 지식을 표현하는 적당한 퍼지규칙을, 전건부와 후건부의 구조 및 매개변수 동조를 통하여, 획득하고, 이 규칙을 새로운 유전자 알고리즘을 이용하여 최적화 시킬 수 있는 새로운 퍼지규칙 획득 알고리즘을 개발한다. 이 방법의 유용성을 입증하기 위하여 물탱크 시스템에 적용한 결과 효과적으로 퍼지규칙을 획득한다.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2007.05a
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• pp.39-50
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• 2007

Sexing from bovine embryos fertilized in vitro implicates a possibility of the sex controlled cattle production. This study was carried out to produce the sex determined cattle through the embryonic sexing by fluorescence in situ hybridization (FISH) technique. FISH was achieved in in vitro fertilized bovine embryos using a bovine Y-specific DNA probe constructed from the btDYZ-1 sequence. Using this probe, a male-specific signal was detected on 100% of Y-chromosome bearing metaphase specimens. The analyzable rate of embryonic sexing by FISH technique was about 93% (365/393) regardless of embryonic stages. As tested single blastomere by FISH and then karyotype with their biopsied embryos, the accuracy of sex determination with FISH was 97.6%. We tried the embryo transfer with sex determined embryos on 15 cattle. Among them, the 5 cattle delivered calf with expected sex last year.

• Clinical and Experimental Reproductive Medicine
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• v.34 no.4
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• pp.293-303
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• 2007

Objective: Previously, we identified differentially expressed genes between GV and MII stage mouse oocytes using ACP technology. When we study one of GV selective genes, Obox family, we found Obox4 mRNA expression in ovaries that has been reported as expressed exclusively in testis. Therefore, this study was conducted for characterization and functional analysis for Obox4. Methods: Expression of Obox4 mRNA was examined in gonads and oocytes by RT-PCR. To determine the role of Obox4 in oocyte maturation, Obox4 dsRNA was microinjected into the cytoplasm of GV oocytes followed by 16 h of incubation in the plain medium or by 24 h of incubation in the medium containing IBMX. After RNAi, phenotypes and maturation rates were observed, change in mRNA expression was evaluated, and chromosomal status was confirmed by orcein staining. Results: Obox4 has minimal expression in the ovary compared to that of the other family members. When oocytes were cultured for 16 h in M16 medium after RNAi, maturation rate was not changed significantly, compared with that of non-injected or buffer-injected control oocytes. Surprisingly, however, when oocytes were cultured for 24 h in M16 containing IBMX, in which oocytes were supposed to arrest at GV stage, Obox4 RNAi oocytes were advanced to MI and MII. Spindle structure was disappeared and the chromosomes were condensed in the oocytes after Obox4 RNAi. Conclusions: This is the first report on the expression of Obox4 in the ovary and oocytes. Results of the study suggest that Obox4 plays a crucial role in spindle formation and chromosome segregation during meiosis in oocytes. In addition, Obox4 may play an important role in cAMP-dependent signal cascades of GV-arrest in mouse oocytes.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.9 no.1
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• pp.165-171
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• 2005

In this paper, the centralized dynamic load-balancing was processed effectively by using the network based parallel genetic algorithm. Unlike the existing method using genetic algorithm, the performance of central scheduler was improved by distributing the process for the searching of the optimal task assignment to clients. A roulette wheel selection and an elite preservation strategy were used as selection operation to improve the convergence speed of optimal solution. A chromosome was encoded by using sliding window method. And a cyclic crossover was used as crossover operation. By the result of simulation for the performance estimation of central scheduler according to the change of flexibility of load-balancing method, it was verified that the performance is improved in the proposed method.

• Proceedings of the KIEE Conference
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• 1999.11c
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• pp.576-578
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• 1999

본 논문에서는 생물학적인 DNA와 유전자 알고리즘의 진화 메커니즘에 근거를 둔 DNA 코딩방법을 변형하여 새로운 DNA 코딩 방법을 제안한다. 이 방법은 기존의 DNA 코딩 방법이 DNA 유전자의 Redundancy와 Over-lapping 성질 때문에 갖고 있는 DNA 자체의 특성인 염색체의 길이를 자유자재로 변화시킬 수 있는 코딩 기술에 진화단계에서 변형을 가할 수 있는 새로운 유전자 알고리즘을 추가하여, 초기에 국소해로 접근하는 일반적인 유전자 알고리즘의 위험 부담률을 줄이고, 전역 해로의 접근 가능성을 높이는 방법을 제시한다. 또한. 이 변형된 DNA 코딩 방법의 가능성을 입증하기 위하여 시스템 제어에 필요한 지식을 표현하는 적당한 퍼지 규칙을 후건부의 매개변수의 동조만을 통하여 획득하고, 이 규칙에 변형된 DNA 코딩 방법을 적용하여 최적화 된 새로운 퍼지규칙 획득 알고리즘을 개발한다. 제안된 알고리즘을 이용한 퍼지 제어기를 설계하고. 이 제어기의 유용성을 입증하기 위하여 병렬형 이중 도립진자 시스템에 적용하여 시뮬레이션을 실행한 결과 효과적으로 퍼지규칙을 획득하고 제어함을 알 수 있다.