Browse > Article
http://dx.doi.org/10.13103/JFHS.2018.33.3.207

Genotoxicity Study of Litsea japonica Fruit Flesh Extract  

Yun, Ji-Hyun (Hurum Central research institute Co., Ltd)
Park, In-Jae (Hurum Central research institute Co., Ltd)
Park, Sung-Hwan (Hurum Central research institute Co., Ltd)
Choi, Goo-Hee (Hurum Central research institute Co., Ltd)
Kim, Hyun-Jung (Hurum Central research institute Co., Ltd)
Cho, Ju-Hyun (Hurum Central research institute Co., Ltd)
Publication Information
Journal of Food Hygiene and Safety / v.33, no.3, 2018 , pp. 207-213 More about this Journal
Abstract
This study aimed to evaluate the genotoxicity of Litsea japonica fruit-hexane extract (LJF-HE). In order to examine the genotoxicity, we carried out bacterial reverse mutation assay, chromosome aberration assay, and a micronucleus induction (MN) test according to the OECD and the Korea Ministry of Food and Drug Safety (MFDS) toxicity test guidelines. In the bacterial reverse mutation assay, no significant increase in revertant colonies, nor bacterial toxicity, was observed in the LJF-HE treatment group, regardless of the absence or presence of metabolic activation by the S9 mixture. However, in the positive control group, revertant colony counts were shown to be more than twice that of the negative control group. The chromosome aberration test showed that the repetition rate of abnormal chromosome aberration was less than 5%, regardless of the treatment time, and with or without the S9 mixture. No significant change was observed when (p < 0.05) compared with the negative control group. The micronucleated polychromatic erythrocytes (MNPCE) repetition rate of the polychromatic erythrocytes (PCE) showed no significant changes when compared with the negative control group (p < 0.05). The PCE portion of total erythrocytes also showed no significant changes (p < 0.05). These results showed that LJF-HE had no significant genotoxic effects.
Keywords
Litsea japonica; Genotoxicity; Reverse mutation; Micronucleus test;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Min B.S., Lee S.Y., Kim J.H., Kwon O.K,. Park B.Y., An R.B., Lee J.K., Moon H.I., Kim T.J., Kim Y.H., Joung H., Lee H.K.: Lactones from the leaves of the Litsea japonica and their anti-complement activity. J. Nat. Prod., 66, 1388-1390 (2003).   DOI
2 Jimenez-Perez N del C., Lorea-Hernandez F.G., Jankowski C.K., Reyes-Chilpa R.: Essential oils in mexican bays (Litsea spp., Lauraceae): Taxonomic assortment and ethnobotanical implications. Econ. Bot., 65, 178-189 (2011).   DOI
3 Tanaka H., Nakamura T., Ichino K.: Butanolides from Litsea japonica. Phytochemistry, 29, 857-859 (1990).   DOI
4 Takeda K., Sakurawi K., Ishii H.: Components of the Lauraceae family-l. New lactonic compounds from Litsea japonica. Tetrahedron, 28, 3757-3766 (1972).   DOI
5 Kim E., Boo H.J., Hyun J.H., Kim S.C., Kang J.I., Kim M.K., Yoo E.S., Kang H.K.: The effect of Litsea japonica on the apoptosis induction of HL-60 leukemia cells. Yakhak Hoeji, 53, 6-11 (2009).
6 Yoon W.J., Kang S.C., Ham Y.M., Kim K.N., Yang W.H., Kim H.J., Park S.Y., Jung Y.H.: Antioxidative and antiinflammatory activities of Litsea japonica leaves. J. Korean Soc. Appl. Biol. Chem., 53, 27-32 (2010).
7 Koo H.J., Yoon W.J., Sohn E.H., Ham Y.M., Jang S.A., Kwon J.E., Jeong Y.J., Kwak J.H., Sohn E., Park S.Y., Jang K.H., Namkoong S., Han H.S., Jung Y.H., Kang S.C.: The analgesic and anti-inflammatory effects of Litsea japonica fruit are mediated via suppression of NF-${\kappa}B$ and JNK/p38 MAPK activation. Int. Immunophamacol., 22, 84-97 (2014).   DOI
8 Namkoong S., Jang S.A., Sohn E.H., Bak J.P., Sohn E., Koo H.J., Yoon W.J., Kwon J.E., Jeong Y.J., Meng X., Han H.S., Kang S.C.: Comparative Study of Litsea japonica Leaf and Fruit Extract on the Anti-inflammatory Effects. Korean J. Plant Res., 28, 145-152 (2015).   DOI
9 Maron D.M., Ames B.N.: Revised methods for the Salmonella mutagenicity test. Mutat. Res., 113, 173-215 (1983).   DOI
10 OECD: OECD guideline testing of chemicals No. 471 Bacterial Reverse Mutation Test (2001).
11 Lee S.S., Park Y.H., Sohn Y., Ryu S.J., Surh Y.J.: Genotoxicity of capsaicin in cultured human lymphocytes. Environ Mutagen. Carcinogen., 15, 81-87 (1995).   DOI
12 Wakata A., Sasaki M.S.: Measurement of micronuclei by cytokinesis-block method in cultured Chinese hamster cells: Comparison with types and rates of chromosome aberrations. Mutat. Res., 190, 51-57 (1987).   DOI
13 Ministry of Food and Drug Safety: Guidelines for toxicity tests of drugs and related materials notification. Chungbuk, MFDS (2014).
14 Kim S.H., Chung M.J.: Safety and Anticancer Effects of Platycodon grandiflorum Extracts. J. Korean Soc. Food Sci. Nutr., 44, 516-523 (2015).   DOI
15 Ham S.S., Hwangbo H.J., Cui C.B., Lee E.Y., Cho M.A., Lee D.S.: Suppressive Effects of Ethanol Extract of Aster scaber Root on Genotoxicity. J. East Asian Soc. Dietary Life, 11, 466-471 (2001).