• 대한방사선방어학회:학술대회논문집
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• 2009.11a
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• pp.112-113
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• 2009

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2005.10a
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• pp.106-106
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• 2005

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2005.10a
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• pp.106-106
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• 2005

• The Science & Technology
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• v.30 no.8 s.339
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• pp.82-83
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• 1997

위암 파라핀조직을 이용하여 세포배양없이 염색체변이를 규명하는 연구로 인정을 받고 있는 카톨릭의대 임상병리학교실 한경자교수. 염색체 연구는 살아있는 세포를 이용해왔으나 한교수는 수술을 통해 얻은 죽은 조직으로 연구해 이 분야의 연구 활성화에 전기를 마련해 학계의 기대를 모으고 있다.

• Proceedings of the Korean Society of Crop Science Conference
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• 1998.04a
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• pp.21-22
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• 1998

• Journal of Life Science
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• v.19 no.4
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• pp.449-456
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• 2009

Murine Neuro-2a (N2a) cells have been widely used for the investigation of neuronal differentiation, trophic interaction and neurotoxic effects of various compounds and their associated mechanisms. N2a cells have many genomic variations such as gains or losses in DNA copy number, similar to other neuroblastoma cells, and no systematic or high-resolution studies of their genome-wide chromosomal aberrations have been reported. Presently, we conducted a systematic genome-wide determination of chromosomal aberrations in N2a cells using a high-throughput, oligonucleotide array-based comparative genomic hybridization (oaCGH) technique. A hidden Markov Model was employed to assign each genomic oligonucleotide to a DNA copy number state: double loss, single loss, normal, gain, double gain and amplification. Unlike most neuroblastoma cells, Mycn amplification was not observed in N2a cells. In addition, these cells showed gain only in the neuron-derived neurotrophic factor (NF), while other neurotrophic factors such as glial line-derived NF and brain-derived NF presented normal copy numbers. Chromosomes 4, 8, 10, 11 and 15 displayed more than 1000 aberrational oligonucleotides, while chromosomes 3, 17, 18 and 19 displayed less than 20. The largest region of gain was located on chromosome 8 and its size was no less than 26.7 Mb (Chr8:8427841-35162415), while chromosome 4 had the longest region of single deletion, with a size of 15.1 Mb (Chr4:73265785-88374165).

• Korean Journal of Plant Taxonomy
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• v.31 no.4
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• pp.311-319
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• 2001

In order to evaluate taxonomic status of Latcuca hallaisanensis H. $L{\acute{e}}v$., an endemic species of the Jeju-do Island, we investigated fruit wall structure and chromosome morphology. The fruit wall structure had 10-11 obtuse costae in the transverse section. The costa was wholly occupied by libriform fiber cells, and the underlying fibersclereid tissue was only one to three cells layers thick. Also, the intercosta lacked fiber-sclereid layers. Somatic chromosome numbers and karyotype of Latcuca hallaisanensis were recorded for the first time. This diploid species (2n=10) with the same basic number of x=5 has the total chromosome length $23.3{\mu}m$ and the length of each chromosome falls in $1.9{\mu}m-2.9{\mu}m$. It possess the karyotype complement i.e., 3sm+2st and a characteristic chromosome pair (No. 1 and 2) with a secondary constriction at the distal portion of the short arms. The overall similarity in external morphology (involucre, achene etc), chromosome morphology as well as in fruit wall anatomy between Lactuca hallaisanensis and Crepidiastrum s. lat. clearly indicated that this species should be treated as Crepidiastrum, rather than Lactuca.

• Korean Journal of Plant Resources
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• v.8 no.1
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• pp.47-54
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• 1995

The ergot fungus requiers a rather long time for the incubation period for a disease and fungus tends to obstract the formation of the seeds. In order to do an experiment inoculated, a spore of ergot fungi into the florets was done 2-3 days before flowering. As a result, each trisomic types recognized the infection rate to be 90% to 100% so the trisomies of complete immunity was non existent in durum wheat, Triticum durum var. hordeiforme. However, the growth rate of large sclerotium than seeds differed mutually with each trisomic types. This tend to be sensitivity on the trisomies which has been the extra chromosomes of 2A, 4A and 7B in comparison with normal plant but the trisomies related to Tri-6A line was not forming the sclerotium of large size than seeds and grew outside the florets. Consequently, the resisitant gene against ergot fungi was considered to be existing on the chromosome 6A. The ergot fungi requires a long time for the incubation period until it is taken away from infection. The essential effect of resistant gene did not surmise on the chromosome 6A that gene concerned with the physiological effect to restrain the growing up of a ergot fungi in the growing process of plants.

• The Korean Journal of Pesticide Science
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• v.8 no.4
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• pp.288-298
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• 2004

Benzimidazole pesticide carbendazim that is effective against a wide range of fungal plant pathogens is a protective, eradicant, and systemic fungicide. For genetic toxicity evaluation of carbendazim on DNA, genes and chromosome, were investigated with chromosome aberration, bacterial reverse mutation, micronucleus test in mouse born marrow and DNA damage assay by single cell microgel electrophoresis. Substitution and frameshift mutation were not induce at variable concentration of carbendazim on Ames test with or without rat liver microsomal activation. For the result of chromosome aberration test, numerical changes of chromosome were detected at the concentrations higher than $4.0{\mu}g/m{\ell}$, but structural aberration was not induced. Positive control, Mitomycin-C and captafol made a structural aberration, but numerical change of chromosome did not appear. In the micronucleus test for mouse born marrow, carbendazim was negative, but was weak positive in DNA damage assay by single cell microgel electrophoresis because of increased DNA moving length of 20% to control.

• Journal of Animal Science and Technology
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• v.51 no.2
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• pp.123-128
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• 2009

Recently, the $2^{nd}$ generation genome map of the Korean cattle (Hanwoo) has been constructed by comparison of the nucleotide sequence of the Korean cattle BAC clones with whole genome sequence of the bovine data-base (B_tau 2.1 build). The objective of this study was to update the $2^{nd}$ generation genome map of the Korean cattle using the similar approach. The nucleotide sequence of the Korean cattle BAC clones utilized in the construction of the $2^{nd}$ generation map was compared with the newly released bovine data-base (B_tau 3.1 build) to generate the $3^{rd}$ generation map. While, 5,105 BAC clones were localized on bovine chromosome in the $2^{nd}$ generation map, a total of 9,595 BAC clones, which spans about 37.27% of the bovine chromosome after eliminating the overlapping sequence among the clones, have been mapped on the bovine chromosome in the $3^{rd}$ generation map. Further analysis of the nucleotide sequence of the BAC clones will allow us to develop map and facilitate to pinpoint the genes that are important for the improvement of the performance in this cattle breed.