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http://dx.doi.org/10.5352/JLS.2009.19.4.449

High Resolution Genomic Profile of Neuro2a Murine Neuroblastoma Cell Line by Array-based Comparative Genomic Hybridization  

Do, Jin-Hwan (HumanGenome Center,Institute of MedicalScience, University of Tokyo)
Kim, In-Su (Departmentof Biotechnology, Konkuk University)
Ko, Hyun-Myung (Departmentof Biotechnology, Konkuk University)
Choi, Dong-Kug (Departmentof Biotechnology, Konkuk University)
Publication Information
Journal of Life Science / v.19, no.4, 2009 , pp. 449-456 More about this Journal
Abstract
Murine Neuro-2a (N2a) cells have been widely used for the investigation of neuronal differentiation, trophic interaction and neurotoxic effects of various compounds and their associated mechanisms. N2a cells have many genomic variations such as gains or losses in DNA copy number, similar to other neuroblastoma cells, and no systematic or high-resolution studies of their genome-wide chromosomal aberrations have been reported. Presently, we conducted a systematic genome-wide determination of chromosomal aberrations in N2a cells using a high-throughput, oligonucleotide array-based comparative genomic hybridization (oaCGH) technique. A hidden Markov Model was employed to assign each genomic oligonucleotide to a DNA copy number state: double loss, single loss, normal, gain, double gain and amplification. Unlike most neuroblastoma cells, Mycn amplification was not observed in N2a cells. In addition, these cells showed gain only in the neuron-derived neurotrophic factor (NF), while other neurotrophic factors such as glial line-derived NF and brain-derived NF presented normal copy numbers. Chromosomes 4, 8, 10, 11 and 15 displayed more than 1000 aberrational oligonucleotides, while chromosomes 3, 17, 18 and 19 displayed less than 20. The largest region of gain was located on chromosome 8 and its size was no less than 26.7 Mb (Chr8:8427841-35162415), while chromosome 4 had the longest region of single deletion, with a size of 15.1 Mb (Chr4:73265785-88374165).
Keywords
Neuroblastoma; chromosomalaberration; Neuro2a; oligonucleotide array-CGH;
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1 Li, X. -H., D. X. D. -X. Long, W. Li, and Y. -J. Wu. 2007. Different mechanisms of lysophosphatidylcholine-induced Ca2+ mobilization in N2a mouse and SH-SY5Y human neuroblastoma cells. Neurosci. Lett. 424, 22-26   DOI   ScienceOn
2 Nakagawara, A., M. Arima, C. G. Azar, N. J. Scavarda, and G. M. Brodeur. 1992. Inverse relationship between trk expression and N-myc amplification in human neuroblastomas. Cancer Res. 52, 1364-1368
3 Nakagawara, A., C. G. Azar, N. J. Scavarda, and G. M. Brodeur. 1994. Expression and function of TRK-B and BNDF in human neuroblastomas. Mol. Cell Biol. 14, 759-767
4 Sakaguchi, M., K. Murayama, K. Yabe, M. Satoh, M. Takeuchi, and E. Matsumura. 1998. Beta-casomorphin-5 stimulates neurite outgrowth in a mouse neuroblastoma cell line (Neuro-2a). Neurosci. Lett. 251, 97-100   DOI   ScienceOn
5 Snider, W. D. 1994. Functions of the neurotrophins during nervous system development: what the knockouts are teaching us. Cell 77, 627-638   DOI   ScienceOn
6 Tonini, G. P. 1993. Neuroblastoma: The result of multistep transformation? Stem Cells 11, 276-282   DOI   ScienceOn
7 van de Wiel, M. A., K. I. Kim, S. J. Vosse, W. N. van Wieringen, S. M. Wilting, and B. Ylstra. 2007. CGHcall: calling aberrations for array CGH tumor profiles. Bioinformatics 23, 892-894   DOI   ScienceOn
8 Ylstra, B., P. van den Ijssel, B. Carvalho, R. H. Brakenhoff, and G. A. Meijer. 2006. BAC to the future! or oligonucleotides: a perspective for micro array comparative genomic hybridization (array CGH). Nucleic Acids Res. 34, 445-450   DOI   ScienceOn
9 Zhang, X., A. S. Vincent, B. Halliwell, and K. P. Wong. 2004. A mechanism of sulfite neurotoxicity: direct inhibition of glutamate dehydrogenase. J. Biol. Chem. 279, 43035-43045   DOI   ScienceOn
10 Aoyama, M., K. Asai, T. Shishicura, T. Kawamoto, T. Miyachi, T. Yocoi, H. Togari, Y. Miura, T. Kato, and A. Nakagawara. 2000, There are different expressions of BDNF and its variants between human neuroblastoma with favorable prognoses and with unfavorable prognoses. Soc. Neurosci. Abstr. 26(Part 2), 2120
11 Brodeur, G. M. , C. Azar, M. Brother, J. Hiemstra, B. Kaufman, H. Marshall, J. Moley, A. Nakagawara, R. Saylors, N. Scavarda, S. Schneider, J. Wasson, P. White, R.Seeger, T. Look, and R. Castleber. 1992. Neuroblastoma: Effect of genetic factors on biology and treatment. Cancer 70, 1685-1694   DOI   ScienceOn
12 Chao, M. V. 1992. Neurotrophin receptors: a window into neuronal differentiation. Neuron 9, 583-593   DOI   ScienceOn
13 Konger, P., G. Barbany, C. Dominici, M. A. Castello, G. Raschella, and H. Persson. 1993. Coexpression of messenger RNA for TRK protooncogene and low affinity nerve growth factor receptor in neuroblastoma with favorable prognosis. Cancer Res. 53, 2044-2050
14 Do, J. H. , I. S. Kim, T. K. Park, and D. K. Choi. 2007. Genome-wide examination of chromosomal aberrations in neuroblastoma SH-SY5Y cells by array-based comparative genomic hybridization. Mol. Cells 24, 105-112
15 Gauvain, J. L. and C. H. Lee. 1992. MAP estimation of continuous density HMM: theory and applications. In DARPA Sp. And Nat. Lang. Workshop. Harriman, New York
16 Johnson, V. J., S. H. Kim, and R. P. Sharma. 2005. Aluminum-maltolate induces apoptosis and necrosis in neuro-2a cells: potential role for p53 signaling. Toxicol. Sci. 83, 329-339   DOI   ScienceOn
17 Lepage, K. T., R. W. Dickey, W. H. Gerwick, E. L. Jester, and D. Murray. 2005. On the use of neuro-2a neuroblastoma cells versus intact neurons in primary culture for neurotoxicity studies. Crit. Rev. Neurobiol. 17, 27-50   DOI   ScienceOn
18 Andersson, R., C. E. Bruder, A. Piotrowski, U. Menzel, H. Nord, J. Sandgren, T. R. Hvidsten, T. Diaz de Stahl, J. P. Dumanski, and J. Komorowski. 2008. A segmental maximum a posteriori approach to genome-wide copy number profiling. Bioinformatics 24, 751-758   DOI   ScienceOn