• The Journal of the Korea Contents Association
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• v.4 no.4
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• pp.45-51
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• 2004

The number of development in educational software is currently decreasing despite rapid improvement in computer technology. This is partly due to the fact that the current software relies on the conventional hypermedia method or on the hierarchical method, both of which have inherent limitations. To compensate their limitations while keeping their merits, a hybrid method called the structured hypermedia link method has recently been proposed in the literature. It has been found that the structured hypermedia link method is far superior than the conventional methods in terms of educational effectiveness because it can help a learner to search various data by navigating between the study topics on his own while maintaining his level of cognitively overloaded stress to the minimum. The clinical microbiology laboratories were chosen as the candidate site for this study because its educational system has not been standardized and has been relying heavily on the personal experience. In this study, the hypermedia link method was applied to the development of an education system for the image analysis in clinical microbiology laboratories. For this purpose, a web-based computer aided instruction(CAI) program was designed to systematically organize the Gram stain method based on the standardized image data. In the future, CAI program must be intended to educate for beginner and developed to accept for variable knowledge. And it will be useful program for technicians in case of applying various examinations based Gram stain method of this study.

• Journal of the Korea Society of Computer and Information
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• v.16 no.10
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• pp.83-92
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• 2011

The aim of this study is to evaluate whether 3D nuclear chromatin texture features are significant in recognizing the progression of cervical cancer. In particular, we assessed that our method could detect subtle differences in the chromatin pattern of seemingly normal cells on specimens with malignancy. We extracted nuclear texture features based on 3D GLCM(Gray Level Co occurrence Matrix) and 3D Wavelet transform from 100 cell volume data for each group (Normal, LSIL and HSIL). To evaluate the feasibility of 3D chromatin texture analysis, we compared the correct classification rate for each of the classifiers using them. In addition to this, we compared the correct classification rates for the classifiers using the proposed 3D nuclear texture features and the 2D nuclear texture features which were extracted in the same way. The results showed that the classifier using the 3D nuclear texture features provided better results. This means our method could improve the accuracy and reproducibility of quantification of cervical cell.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.20 no.8
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• pp.417-424
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• 2019

Despite its superior ability to show distinct cellular morphology and for long-term storage, conventional tissue fixation by formalin has many drawback, including slower fixation, the exposure to harmful chemicals and extensive protein modification. Herein, we assessed the effects of rapid microwave-assisted tissue fixation on histological examination and on protein integrity by comparing these microwave irradiation fixated tissues with the formalin-fixed tissues. One of the paired mouse tissues (liver and kidney) was fixed in formalin and the other was fixed by using microwave irradiation in phosphate buffered saline. Each slide from the paraffin-embedded tissues was examined by H & E staining for the adequacy of fixation and by immunohistochemical staining for antigenicity in a blinded fashion. Evaluation of protein recovery and the protein quality from the fixed tissues were analyzed by the BCA method and Western blotting, respectively. The results from H & E staining and immunohistochemical staining showed that the sections obtained from microwave-fixed tissues under our experimental conditions were comparable to those of the formalin-fixed tissues except for the integrity of RBCs. Furthermore, proteins were effectively extracted from the microwave-fixed tissues with acceptable preservation of the proteins' quality. Taken together, this microwave-assisted tissue processing yields a quick fixation and better protein recovery in higher amounts, as well as the adequacy of fixation and the antigenicity being comparable to formalin-fixed tissues, and this all suggests that this new fixation technique can be applied in an environment where rapid tissue fixation is required.

• Journal of Bio-Environment Control
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• v.18 no.4
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• pp.413-419
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• 2009

The appropriate dripper position in perlite bag was investigated for tomato production. Drippers were laid at 5(F5), 15cm (F15) away from the stem base or 5cm at first and then moved to 15cm later (M5-15). Roots were developed more near the stem base in F5, while less in F15. Roots were distributed evenly in M5-15. In vertical distribution of water in perlite bag, water content was higher as it went deeper with the variation by dripper positions. Yield was high in F15 and low in F5. In conclusion the position of dripper is the best at 15cm from the stem base in perlite bag culture in view of root distribution and yield.

• Journal of the Korean Home Economics Association
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• v.42 no.6
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• pp.43-53
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• 2004

The purpose of this paper is to investigate the dyeability, antibacterial activity, and deodorization of cotton and silk fabrics dyed with extracted solution from Chrysanthemum boreale. The results are as follows： 1. The optimum conditions of dyeing temperature and time for the cotton fabrics were 90$^{\circ}C$ and 60minutes, while those of the silk fabrics were 60$^{\circ}C$ and 30 minutes respectively. 2. The K/S values of cotton fabrics were slightly higher than those of silk fabrics. The K/S values of both fabrics were slightly increased by mordanting with Cr but were decreased by mordanting with Cu and Fe 3. The colors of the cotton and silk fabrics changed from GY to Y. In both cases, the values of the fabric colors were lowered, and the values of the colors continued to be lowered with Cu and Fe. The chroma and color difference of both fabrics were high and were further increased by mordanting with Cu. 4. The fastness to dry-cleaning and rubbing of the cotton and silk fabrics was very good. The fastness to washing of both fabrics was good except when mordanted with Fe. The fastness to perspiration of both fabrics was fairly good, but the fastness to acid perspiration was poorer than that to the alkaline perspiration. The fastness to light of both fabrics was very poor. 5. The cotton and silk fabrics showed excellent antibacterial activity. Both fabrics mordanted with Cu showed perfect antibacterial activity. 6. The deodorization of the silk fabrics was very good, and that of the silk fabrics mordanted with Cu and Al was excellent, while the deodorization of the cotton fabrics was fairly good.

• Korean Journal of Food Science and Technology
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• v.9 no.4
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• pp.271-276
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• 1977

Treating with step concentration of papain, round mucle of Korean cattle were cut in longitudinal and cross section and stained. Collagenous fibre and elastic fibre of its connective tissue were observed microscopically. The results were as follows: 1) In proportion to the increase of enzyme concentration amorphous bundle of collagenous fibre were loosed gradually and destroyed in the long run and besides the property of this fibre stained became remarkably weak. 2) Elastic fibre was paralleled to muscle fibre and in proportion to the increase of enzyme concentration, it was lost elasticity, loosed, straightened and broken remarkably to pieces. 3) Histological variation of collagenous fibre and elastic fibre treated with enzyme was more remarkable than control.

• Proceedings of the Korean Society of Dyers and Finishers Conference
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• 2012.03a
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• pp.72-72
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• 2012

본 연구에서는 친환경 리싸이클 섬유산업의 활성화를 위해 대학생들의 리싸이클 폴리에스터 섬유에 대한 인식도를 분석해 보고자 하였다. 측정 문항은 섬유전문가 3인의 검토를 거쳐 타당성을 확보하였으며, 조사는 질문지법으로 2011년 9월 1일부터 9월 10일까지 대전 거주 대학생을 대상으로 진행하였으며, 회수된 설문지 196부를 자료 분석에 이용하였고, SPSS 19 통계 패키지를 이용하여 자료를 통계분석하였다. 연구 결과는 다음과 같다. 1. 친환경적 의생활 실태 조사를 실시한 결과, 리싸이클 PET섬유에 대해 들어본 적이 있는 사람은 조사대상자의 27.6%로 나타났고, 그 정보원으로는 인터넷을 가장 많이 이용하는 것으로 나타났다. 2. 섬유소재별 친환경성에 대한 인식도를 조사한 결과, 대학생들은 리싸이클 PET섬유가 천연섬유보다 덜 친환경적이나, 인조섬유보다는 더 친환경적이라고 인식하는 것으로 나타났다. 3. 리싸이클 PET섬유에 대한 인식도를 요인분석한 결과, <친환경성 요인>, <촉감 요인>, <내구성 요인>, <기능성 요인>, <공정 요인>, <글로벌 경쟁력 요인>및 <부가가치성 요인> 등 7개의 요인이 도출되었는데 이는 대학생들은 리싸이클 PET섬유에 대해 친환경성, 촉감, 내구성, 기능성, 공정, 글로벌경쟁력, 부가가치성순으로 중요하게 인식하는 것으로 해석된다.

• Journal of Pharmacopuncture
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• v.11 no.2
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• pp.5-12
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• 2008

Objective In this article, we report on the intradermal Alcian blue staining method for tracing the meridians of acupuncture. Methods 1% Alcian blue solution was injected into acupoints by using a 0.5mL insulin syringe with a 31-gauge needle, then the skin was incised and was observed under a stereoscopic microscope. The specimens were examined by using immunohistochemical methods and were observed under a confocal laser scanning microscope. Results A threadlike structure, which was visualized with Alcian blue, existed in dermis layer and proceeded to hypodermis. In this structure, characteristic alignments of rod- shaped nuclei and $1-2{\mu}m$ sized DNA granules were observed. Furthermore, abundant blood capillary plexuses, peripheral nerve endings, and a corpuscle-like structure(about $300{\mu}m$ in diameter) were visualized in the skin tissues of acupoints. Conclusion It was concluded that the specific threadlike and corpuscle-like structures corresponded to superficial Bonghan duct and corpuscle, respectively.

• Journal of Yeungnam Medical Science
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• v.6 no.2
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• pp.23-28
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• 1989

After application of silver nitrate on every newborn, the incidence of gonococcal conjunctivitis was markedly decreased. But recently neonatal conjunctivitis due to chlamydia infection is increasing, so clinical observation was made on 26 newborn infants who showed eye discharge from June 1st to August 31st 1989. The results were as follows. 1. The incidence of chlamydia infection among neonatal Conjunctivitis was 34.6%. 2. The most common age at diagnosis was 6-15days of life and there was no sexual prepondrance. 3. There was no significant difference on clinical symptoms between chlamydia conjunctivitis and other Conjunctivitis. 4. Of 26 infants examined. 16 cases revealed no growth on routine bacterial culture. Of the organism cultured, P. aeruginosa was the most common agent(19.2%) and followed by S. aureus(11.5%) and S. epidermidis(7.6%). In one case of chlamydia I conjunctivitis, there was concurrent S. aureus infections. 5. On this study, Giemsa stain did not give significant diagnostic aid of chlamydia conjunctivitis.

• Journal of Yeungnam Medical Science
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• v.8 no.2
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• pp.63-69
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• 1991

Nucleolar organizer regions (NOR) are loops of ribosomal DNA(rDNA) which are transcribed by RNA polymerase I. They produce ultimately ribosome and protein. Thus they are believed to reflect nuclear activity. We applied silver colloid staining technique to human glioma to examine relationship between the mean number of Ag-NOR and histopathological grading. The mean number of Ag-NOR(${\pm}$ S.E of the mean)were $1.17{\pm}0.07$ in normal brain, $1.53{\pm}0.25$ in astrocytoma, $2.37{\pm}0.71$ in malignant astrocytoma, and $2.88{\pm}0.41$ in glioblastoma multiforme. And there was a statistically significant difference among these. The results show that Ag-NOR technique is a rather simple and rapid method and will become a helpful tool for estimation of the proliferative potential of glioma.