Journal of Pharmacopuncture (대한약침학회지)

KOREAN PHARMACOPUNCTURE INSTITUTE (대한약침학회)
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Intradermal Alcian-Blue Injection Method to Trace Acupuncture Meridians

경락추적을 위한 피내 알시안 블루 주입방법

  • Sung, Baeck-Kyoung (Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University) ;
  • Kim, Min-Su (Department of Veterinary Surgery, College of Veterinary Medicine, Chonbuk National University) ;
  • Ogay, Vyacheslav (Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University) ;
  • Kang, Dae-In (Korean Pharmacopuncture Institute) ;
  • Soh, Kwang-Sup (Biomedical Physics Laboratory, Department of Physics and Astronomy, Seoul National University)
  • 성백경 (서울대학교 물리.천문학부 한의학물리연구실) ;
  • 김민수 (전북대학교 수의과대학 수의외과학 교실) ;
  • 오가이 (서울대학교 물리.천문학부 한의학물리연구실) ;
  • 강대인 (대한약침학회) ;
  • 소광섭 (서울대학교 물리.천문학부 한의학물리연구실)
  • Published : 2008.06.30
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Abstract

Objective In this article, we report on the intradermal Alcian blue staining method for tracing the meridians of acupuncture. Methods 1% Alcian blue solution was injected into acupoints by using a 0.5mL insulin syringe with a 31-gauge needle, then the skin was incised and was observed under a stereoscopic microscope. The specimens were examined by using immunohistochemical methods and were observed under a confocal laser scanning microscope. Results A threadlike structure, which was visualized with Alcian blue, existed in dermis layer and proceeded to hypodermis. In this structure, characteristic alignments of rod- shaped nuclei and $1-2{\mu}m$ sized DNA granules were observed. Furthermore, abundant blood capillary plexuses, peripheral nerve endings, and a corpuscle-like structure(about $300{\mu}m$ in diameter) were visualized in the skin tissues of acupoints. Conclusion It was concluded that the specific threadlike and corpuscle-like structures corresponded to superficial Bonghan duct and corpuscle, respectively.

목적 : 본 논문에서는 경락을 추적하기 위한 피내(皮內) 알시안 블루(Alcian blue) 염색 방법을 기술한다. 방법 : 1% 알시안 블루 용액을 31게이지 바늘이 달린 0.5mL 인슐린 주사기를 사용하여 경혈 지점에 피내 주사한 후 실체 현미경하에서 수술, 관찰하였다. 면역조직화학적 방법을 사용하여 해당 조직을 레이저 공초점주사현미경으로 관찰하였다. 결과 : 알시안 블루로 염색되고 피하로 이어지는 실 모양 구조물을 관찰하였다. 이 조직 내에서 특징적인 막대모양 핵 배열 및 $1-2{\mu}m$크기의 데옥시리보핵산 입자가 관찰되었다. 또한 경혈 조직 내에서 풍부한 모세혈관총 및 말초신경 말단, 그리고 약 $300{\mu}m$크기의 소체형 구조물을 확인하였다. 결론 : 경혈 지점의 피내에서 발견된 특징적 실 모양 구조물 및 소체형 구조물은 각각 표층 봉한관 및 소체로 판단된다.

Keywords

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