• Journal of Applied Biological Chemistry
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• v.63 no.4
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• pp.443-449
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• 2020

This study was performed to investigate total phenolic contents, anti-oxidant, biological disease-related enzyme inhibitory, and anti-microbial effects of extracts of Lonicera morrowii leaves prepared with water and 40% ethanol. Anti-oxidative activities of the extracts increased in a dose-dependent manner and were very high even at low phenolic concentration. At phenolic concentrations ranging between 50 and 200 ㎍/mL, the water and ethanol extracts inhibited 39.34-76.35 and 47.53-99.83% of xanthine oxidase activity, 30.21-79.06, and 59.40-87.14% of angiotensin converting enzyme activity, and 59.81-80.35 and 36.06-63.58% of α-glucosidase activity, respectively. All inhibitory effect were increased in a dose-dependent manner. In the paper disc agar diffusion assay against seven microbes, L. morrowii extracts had anti-bacteria activities against Helicobacter pylori, Propionibacterium acne but no effect against other bacteria. These results indicate that the extracts from L. morrowii leaves have the possibility to be developed as a physiologically functional source for prevention of adult diseases.

• Korean Journal of Plant Resources
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• v.29 no.4
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• pp.355-362
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• 2016

We investigated the effects of Acorus gramineus water extract on the blood lipid levels in high fat diet-fed obese male mice. We divided thirty-five C57BL/6 mice into 5 groups: normal group, control group, and groups treated with Acorus gramineus water extract at concentrations of 20, 100, and 500 ㎎/㎏. We inoculated Acorus gramineus water extract per orally once a day for 6 weeks respectively. The results revealed that Acorus gramineus water extracts had positive effects on the total cholesterol, triglyceride, high-density lipoprotein cholesterol, and low-density lipoprotein cholesterol levels.

• Food Science of Animal Resources
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• v.26 no.1
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• pp.136-143
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• 2006

We examined the macrophage activity of Codonopsis lanceolata and its effect on the growth of lactic starter culture when it was added to fermented milk. Nitric oxide(NO) and interleukin-1${\alpha}(IL-1{\alpha})$ were increased significantly(p<0.05) by addition of Codonopsis lanceolata water extract at $1,000\;{\mu}g/mL$. Tumer necrosis factor-${\alpha}(TNF-{\alpha})$ was increased significantly (p<0.05) by addition of Codonopsis lanceolata water extract or 70% ethanol extract at $1,000\;{\mu}g/mL$. Water extract of Codonopsis lanceolata showed higher macrophage activity than 70% ethanol extract. Growth of lactic starter culture was inhibited by the increased addition of Codonopsis lanceolata water extract, resulting in less decrease in pH. A stirred type or drink type fermentation process seemed mote suitable than a set type in proper production of Codonopsis lanceolata extract added fermented milk.

• Korean Journal of Food Science and Technology
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• v.33 no.5
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• pp.626-632
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• 2001

The plant extracted from Nameko, Gallic, Green tea, Allspice, Polygonum multiflorum, Schizandra chinensis, Armeniacae and Pine needle were utilized to investigate the effects of extracts on free radical reaction, lipid oxidation and nitrite scavenging ability. The pH of ethanol extracts showed a higher than that of hot water extracts, among of which were showed the lowest pH 3.0 in Schizandra chinensis. The important factor of lipid oxidation were $Fe^{2+}$ ion and active oxygen, in which were bound by plant extracts in case of $Fe^{2+}$ ion existed. However, the hydroxyl radical scavenging ability of extracts were lowed, compared to extracts reacted with $Fe^{2+}$ ion. Among of them, the hydroxyl radical scavenging ability of Nameko and Pine needle extracts had a lower TBARS value than those of control. The iron content of extracts were less than 2.0 mg/100 g, but the total iron content of Schizandra chinensis extracts were 6.8 mg/100 g. The ethanol extracts of pine needle were higher than those of hot water extracts on the basis of $Fe^{2+}$ ion content. The ascorbic acid content of green tea showed 14.3 mg/100 g in hot water extracts and 16.7 mg/100 g in ethanol extracts. Electron donating ability of extracts showed more than 50%, except Nameko and allspices, which were higher in ethanol extracts than those of hot water extracts. The superoxide dismutase(SOD)-like activity of green tea showed 85.3% and 63.5% in hot water and ethanol extracts, respectively. The nitrite scavenging ability of green tea was the most effective in both extracts.

• Journal of Life Science
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• v.24 no.11
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• pp.1217-1223
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• 2014

This study was attempted to investigate antioxidant activities of water and ethanol extract from Rosa multiflora (RM) by in vitro assays measuring 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, 2,2'-azino-di-2-ethyl-benzthiazoline sulphonate (ABTS) radical scavenging activity, reducing power activity, nitric oxide (NO) radical scavenging activity, total phenol and total flavonoid content. The water and ethanol extracts from RM scavenged the DPPH radical and ABTS radical in a dose-dependent manner at the concentration range from 10 to $500{\mu}g/ml$. The DPPH radical scavenging activity of water extract was higher than that of ethanol extract. $IC_{50}$ of DPPH radical scavenging activity of water and ethanol extract were $79.73{\mu}g/ml$ and $145.85{\mu}g/ml$. The reducing power activity of water extract was higher than that of ethanol extract. The nitric oxide (NO) radical scavenging activity of the RM extract was similar to their DPPH radical scavenging activity. $IC_{50}$ of ABTs radical scavenging activity of water and ethanol extract was $79.82{\mu}g/ml$ and $159.03{\mu}g/ml$. The reducing power activity of water extract (0.775) was higher than that of ethanol extract (0.568). Total phenolic content of water extract (140.74 mg/g) was higher than that of the ethanol extracts (37.83 mg/g). Total flavonoid content of water extract (45.31 mg/g) was higher than that of the ethanol extracts (42.68 mg/g). This results suggest that water extract of RM may be useful as potential antioxidant sources.

• KSBB Journal
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• v.19 no.2
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• pp.113-117
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• 2004

The effect of ultrasonification on immuno-stimulatory activities of the extracts from Ephedra sinica Stapf and Rubus coreanus Miq. was observed by the growth of human immune cells. The extraction yields from water extracts and water extract with ultrasonification at 40$^{\circ}C$, 60$^{\circ}C$ and 100$^{\circ}C$ were compared. The highest yield was observed enhancement ratio 2.34% in the extract at 40$^{\circ}C$ with ultrasonification. The growth of human T-cell was increased up to 13.8${\times}$10$^4$ viable cells/$m\ell$ in adding 1.0 g/L of the ultrasonification extracts. The treated extracts increased the specific secretion of TNF-${\alpha}$ up to 10.5${\times}$10$\^$-4/ pg/cell. It was suggested that of the extracts on water the extraction yield with ultrasonification compared improved the extraction with ultrasonification of low than.

• Journal of the Korean Society of Food Science and Nutrition
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• v.39 no.8
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• pp.1179-1186
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• 2010

The antiradical property of hot water extract from dried radish (DR) or dried radish roasted with pressure (DRRP) was investigated in vitro and in LLC-PK1 cell system. The contents of total free amino acid and reducing sugar in DR were decreased by 72.86% and 3.17%, respectively, after pressurized roasting. In vitro test, $IC_{50}$ for DR and DRRP for DPPH radical scavenging activity were 646.70 and $135.45\;{\mu}g/mL$, 896.10 and $566.98\;{\mu}g/mL$ for superoxide anion radical, and 722.26 and $531.84\;{\mu}g/mL$ for hydroxy radical, respectively. The radical scavenging effects of DRRP was significantly greater than those for DR (p<0.001). These radical scavenging effects of DR and DRRP were confirmed in LLC-$PK_1$ at which oxidative stresses were induced by superoxide, nitric oxide and peroxynitrite generated in the treatment of pyrogallol, SNP, and SIN-1, respectively. Cell viability was increased in the presence of DR or DRRP, dose dependently (p<0.05), and TBARS formation was decreased. The protective effects of DRRP against oxidative damage in LLC-$PK_1$ were greater than those of DR at the same concentration tested (p<0.05). This superior antiradical activity of DRRP might be due to the products produced during the pressurized roasting in addition to the antioxidative compounds originally present in the radish. 5-hydroxyl methyl furfural (5-HMF) known as an intermediate product of the maillard reaction was detected in DRRP (0.57 mg/g), but not from DR. In conclusion, daily consumption of DRRP may prevent oxidative damage by retarding oxidative stress.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.101-101
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• 2019

나고야 의정서가 국내에 본격 시행됨에 따라 전남산림자원연구소는 도내 유용 산림 자원의 주권 확립 및 자원 정보의 DB 구축을 위하여 산림생명자원 추출물은행을 운영 중에 있다. 그 일환으로 1차년 난대 수종 4종(동백, 비자, 편백, 황칠)의 부위(잎, 가지) 시기(1차:4월, 2차:7월, 3차:9월, 4차:11월) 용매별(열수, 에탄올) 추출물을 제작하여, 유효성분 및 생리활성의 분석을 실시하였다. 총 폴리페놀(Total Polyphenol) 함량을 150 ug/g 이상을 기준으로 분석 분류한 결과, 동백 잎(4차) 열수, 가지(1, 3차), 잎(4차) 에탄올, 비자 잎(3, 4차) 열수, 가지(2, 3차), 잎(1, 3, 4차) 에탄올, 편백 가지(3차) 열수, 잎(3차), 가지(2, 3차) 에탄올, 황칠 잎(2, 3, 4차) 에탄올 추출물에서 높은 함량의 총 폴리페놀을 확인 할 수 있었다. 다음으로, 프리라디칼(DPPH) 소거능을 측정하여 50 % 이상을 기준으로 분류한 결과, 동백(1~4차), 가지(1~3차) 열수, 잎(1~4차), 가지(1, 3, 4차) 에탄올, 비자 잎(3차) 열수, 잎(2, 4차) 에탄올, 편백 잎(3, 4차), 가지(1차) 열수, 잎(1~3차), 가지(1, 3, 4차) 에탄올, 황칠 잎(2~4차) 열수 추출물에서 높은 프리라디칼(DPPH) 소거능을 보여 주었다. 이와 같이, 수종별로 부위 시기 용매별 추출물에 따른 유효성분과 생리활성에 차이를 확인 할 수 있었다. 이를 바탕으로 산업화 활용 시 채취 시기 및 부위를 특정 하는 자료로 도움 될 것으로 생각되며, 지속적으로 연계 추출 및 분석을 통해 보다 유의성 있는 자료 수집을 계속 진행 중 입니다. 이러한 산림생명자원 추출물은행의 연구 결과를 바탕으로 보다 심도 있게 반복적인 데이터를 수집 구축하고, 연구개발에 활용한다면, 고부가가치 임산 자원을 개발 및 산업화에 큰 도움이 되고 임업임 소득증대에 기여할 것이다.

• Journal of radiological science and technology
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• v.28 no.4
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• pp.333-340
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• 2005

In this study, the radioprotective effects of Grifola umbellata hot water extracts (Gu-extract) on mice were investigated. Single pre-administration of Gu-extract increased the 40-day survival ratio of irradiated mice from 65.5％ to 78.6％. The growth of 3 week old male mice in the irradiated group was slightly retarded as compared to those of the control and Gu-extract treated mice. The average spleen and thymus weights of the irradiated mice were lower than those of the control and Gu-extract treated mice. The weight reduction of testis in the irradiated mice was significant. While it was relatively slight in the Gu-extract treated mice as compared to that of control mice. No significant difference in the weight was observed in heart, kidney or liver among three groups. The leukocytes of the Gu-extract treated mice did not decrease dramatically as in the irradiated group, but recovery patterns were similar in both groups. Reduction of erythrocytes were similar in both groups but its recovery occurred more rapidly in the extract treated group. The glucose level of the Gu-extract treated group did not change during the period examined, while it was still higher in the irradiated group than the level in the control group in two weeks. The cholesterol levels in the irradiated and the Gu-extract treated groups were higher than that of control group on day 7, but decreased to the level of the control group on day 14. No difference was observed in total protein amount of the serum among the three groups. SDS-polyacrylamide gel electrophoresis of the soluble proteins extracted from various organs did not reveal differences to any extent in all groups except in the livers of the irradiated and extract treated groups, in which some proteins were missing or less present.

• Journal of Digital Convergence
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• v.19 no.4
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• pp.365-371
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• 2021

In order to verify the effect of using functional cosmetic ingredients for skin beauty, the composition and content of some phenolic acid and flavonoids in the hot water and 80% methanol extract of Akebia quinata fruit pericarp and seeds were analyzed, and the skin care antioxidant activity was investigated. The results are as follows. Total polyphenol and flavonoid contents were found to be higher in pericarp extract than seed extract in both hot water and 80% methanol extract. In the analysis of the composition and content of phenolic acid and flavonoids, two kinds of phenolic acids were identified in the hot water extract of pericarp, 6 kinds of phenolic acids were identified in the 80% methanol extract, and one kind of flavonoid. Two types of phenolic acids were identified in the hot water and 80% methanol extract of seeds, respectively. DPPH and ABTS radical scavenging activities tended to increase in proportion to the treatment concentration in both hot water and 80% methanol extracts, and antioxidant activity was found to be high. Therefore, from the above results, it was found that the hot water and 80% methanol extract of Akebia quinata fruit pericarp and seeds contained various kinds of phenolic acid and flavonoids, and the antioxidant activity was also high. It is believed to be of value as a natural antioxidant in skin care cosmetic ingredients.