• Title/Summary/Keyword: 열분석적 특성

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Isolation and Optimized Culture Conditions of Fibrinolytic Enzyme Producing Strain Isolated from Korean Traditional Soybean Sauce (간장 유래 혈전분해 효소 생산 균주의 분리 및 배양학적 특성)

  • Baek, Seong-Yeol;Yun, Hye-Ju;Park, Heui-Dong;Yeo, Soo-Hwan
    • Microbiology and Biotechnology Letters
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    • v.39 no.4
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    • pp.330-336
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    • 2011
  • Bacterial strains exhibiting fibrinolytic activity were screened from traditional Korean soybean sauce. The Fibrinolytic activities of the various isolated microorganism were further examined and the superior strain YJ11-21 was selected for further analyses. Gene sequence analysis of 16S rDNA of the YJ11-21 strain revealed Bacillus licheniformis. Optimal culture conditions were investigated in order to maximize the production of the fibrinolytic enzyme by YJ11-21. Amongst the carbon sources tested, glucose was the most effective for enzyme production and amongst the nitrogen sources tested, yeast extract was seen to be the most effective. A one percent addition of NaCl to the medium resulted in the highest fibrinolytic activity. Interestingly, a 10% addition of NaCl resulted in a high activity together with a high cell growth rate. Therefore, YJ11-21 is speculated of being a halotolerant. The optimum pH and temperature for enzyme production were a pH of 9.0 and $30^{\circ}C$, respectively.

An Antifungal Subatance, 2,4-Diacetylphloroglucinol Produced from Antagonistic Bacterium Pseudo-monas fluorescens 2112 Against Phytophthora capsici (Phytophthora capsici를 길항하는 Pseudononas fluorescens 2112가 생산하는 항진균 항생물질 2,4-diacetylphloroglucinol)

  • 이은탁;김상달
    • Microbiology and Biotechnology Letters
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    • v.29 no.1
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    • pp.37-42
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    • 2001
  • An antifungal substance was purified from culture broth of Pseudomonas flulorescens 2112 that showed a broad-spectrum antagonistic activity against various phytopathogenic fungi including capsici. The substance was identified as 2,4-diacetylphloro-glucinol basd on NMR analysis. The 2,4-diacetylphloroglcinol showed antibiotic activity in broad acidic range from pH 1.0 to pH 9.0. About 83% of initial activity was remained after incubation for 30min ar $60^{\circ}C$, however, the activity was dropped up to 50% after 30 min incubation in $80^{\circ}C$. When the nucleotides of P. capsici treated with 2,4-diacetylphloroglucinol were labeled with[$^{3}$ H]-Adenin, the newly synthesized and radioactive-labeled RNA was significantly reduced than those of untreated P. capsici. indicating that the 2,4-diacetylphloroglucinol inhibits RNA synthesis.

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Evaluating the groundwater prediction using LSTM model (LSTM 모형을 이용한 지하수위 예측 평가)

  • Park, Changhui;Chung, Il-Moon
    • Journal of Korea Water Resources Association
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    • v.53 no.4
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    • pp.273-283
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    • 2020
  • Quantitative forecasting of groundwater levels for the assessment of groundwater variation and vulnerability is very important. To achieve this purpose, various time series analysis and machine learning techniques have been used. In this study, we developed a prediction model based on LSTM (Long short term memory), one of the artificial neural network (ANN) algorithms, for predicting the daily groundwater level of 11 groundwater wells in Hankyung-myeon, Jeju Island. In general, the groundwater level in Jeju Island is highly autocorrelated with tides and reflected the effects of precipitation. In order to construct an input and output variables based on the characteristics of addressing data, the precipitation data of the corresponding period was added to the groundwater level data. The LSTM neural network was trained using the initial 365-day data showing the four seasons and the remaining data were used for verification to evaluate the fitness of the predictive model. The model was developed using Keras, a Python-based deep learning framework, and the NVIDIA CUDA architecture was implemented to enhance the learning speed. As a result of learning and verifying the groundwater level variation using the LSTM neural network, the coefficient of determination (R2) was 0.98 on average, indicating that the predictive model developed was very accurate.

Field Applicability of Low Temperature Thermal Desorption Equipment through Environmental Impact Analysis of Remediated Soil and Exhaust Gas (정화토양 및 배출가스의 환경적 특성 분석을 통한 저온열탈착장치의 현장 적용성 평가)

  • Oh, Cham-Teut;Yi, Yong-Min;Kim, Young-Soung;Jeon, Woo-Jin;Park, Gwang-Jin;Kim, Chi-Kyung;Sung, Ki-June;Chang, Yoon-Young;Kim, Guk-Jin
    • Journal of Soil and Groundwater Environment
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    • v.17 no.3
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    • pp.76-85
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    • 2012
  • Geochemical and ecological properties of remediated soil and gas exhausted from a low-temperature thermal desorption (LTTD) process were analyzed to assess the environmental impact of LTTD treatment. Soil characteristics were examined with regard to the chemical (EC, CEC, and organic matter) and the ecological (dehydrogenase activity, germination rate of Brassica juncea, and growth of Eisenia andrei) properties. The exhaust gases were analyzed based on the Air Quality Act in Korea as well as volatile organic compounds (VOCs) and mixed odor. Level of organic Organic matter of the soil treated by LTTD process was slightly decreased compared to that of the original soil because the heating temperature ($200^{\circ}C$) and retention time (less than 15 minutes) were neither high nor long enough for the oxidation of organic matter. The LTTD process results in reducing TPH of the contaminated soil from $5,133{\pm}508$ mg/kg to $272{\pm}107$ mg/kg while preserving soil properties. Analysis results of the exhaust gases from the LTTD process satisfied discharge standard of Air Quality Law in Korea. Concentration of VOCs including acetaldehyde, propionaldehyde, butyraldehyde and valeraldehyde in circulation gas volatilized from contaminated soil were effectively reduced in the regenerative thermal oxidizer and all satisfied the legal standards. Showing ecologically improved properties of contaminated soil after LTTD process and environmentally tolerable impact of the exhaust gas, LTTD treatment of TPH-contaminated soil is an environmentally acceptable technology.

Evaluation of the corrosion property on the welded zone of seawater pipe by A.C shielded metal arc welding (교류 피복아크 용접에 의한 해수 배관 용접부위의 부식 특성 평가)

  • Jeong, Jae-Hyun;Kim, Yun-Hae;Moon, Kyung-Man;Lee, Myeong-Hoon;Kim, Jin-Gyeong
    • Journal of Advanced Marine Engineering and Technology
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    • v.37 no.8
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    • pp.877-885
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    • 2013
  • A seawater pipe of the engine room in the ships is being surrounded with severely corrosive environments caused by fast flowing of the seawater, containing aggressive chloride ion and high conductivity etc.. Therefore, the leakage of the seawater from its pipe have been often occurred due to its local corrosion by aggressive chloride ions. Subsequently, its leakage area is usually welded by AC shielded metal arc welding with various electrodes. In this study, when the sea water pipe is welded with several types of electrodes such as E4301, E4311, E4313 and E4316, a difference of the corrosion resistance on the welding metal zones was investigated using an electrochemical method, observing microstructure, measuring polarization behaviors and hardness. The weld metal zone welded with E4313 electrode exhibited the lowest value of hardness compared to other weld metal zones. In addition, its zone indicated also the best corrosion resistance than those of other weld metal zones. Furthermore, all of the weld metal zones revealed a relatively better corrosion resistance than those of the base metal zones. and also showed higher hardness than the base metal zones.

Comparison of NSC system in the U.S., Japan, and the Republic of Korea (NSC(국가안전보장회의) 체제의 한미일 비교)

  • Kwon, Hyuck-Bin
    • Korean Security Journal
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    • no.37
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    • pp.29-50
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    • 2013
  • With the recent global threats of terrorism as well as religious conflicts, Northeast Asian countries including South Korea, China, and Japan are experiencing particularly serious security crises as demonstrated by North Korea's threats of nuclear weapons testings and long-range missile launching as well as military provocation toward South Korea such as sinking of ROKS Cheonan and bombardment of Yeonpyeong island and the territorial dispute between China and Japan over Senkaku Islands(Diaoyu Islands). As a result, Park Geun Hye Administration of South Korea and the 2nd Abe Shinzo Cabinet of Japan, both recently established, are making efforts to improve their national security and crisis management policies. One of the key elements of such efforts is the strengthening of National Security Council(NSC) or its equivalent organization as the control tower of national security policy, modeled after the NSC of the United States. This paper compares NSC organization of Korea, the U.S., and Japan and draws policy insights focusing on the current political and national security situation South Korea is facing. Although organizational structure, function, and history of NSC of each country differs, it can be inferred from this comparison that NSC-type of organizations can play an important role as a control tower of security and emergency management policies.

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Development of a Simulation Program for the Li-Reduction Process of PWR Spent Fuel (PWR 사용후핵연료의 Li 환원과정 모사 프로그램 개발)

  • Lee, Yun-Hee;Shin, Hee-Sung;Jang, Ji-Woon;Kim, Ho-Dong;Yoon, Ji-Sup
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.4 no.4
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    • pp.335-344
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    • 2006
  • In this paper a computer program was developed, which simulates the Li reduction process of PWR spent fuel, and the amount of a produced metal or chloride compound was calculated at the various amount of Li with the program. It establishes a database, which is composed of some characteristics related to a chemical reaction equation and thermodynamic data, and it calculates the transformed rate of PWR spent fuel oxide at the certain amount of Li by using the database as input data. As the results of the performance test of the program, it was validated that the transformed values of oxides, except for $Eu_2O_3$ and $Sm_2O_3$, were almost the same to within about a 6 % error with those calculated by the previous code and that the calculated amount of Li was also exactly consistent with the theoretical one, which is used for a complete reaction of each oxide in a single chemical reaction. A relationship between Li and the transformed metal of each oxide was analyzed on the basis of the quantities calculated with the verified development program. Of the results, when the amount of Li was given to be 250 mole, the 83.73 percentage of $UO_2$ was transformed into U while the remainder was still to be $UO_2$. In addition, it was appeared that the 297 mole of Li was needed to completely convert $UO_2$ into U.

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Quasi-Static Test for Seismic Performance of Circular Hollow RC Bridge Pier (원형 중공 콘크리트 교각의 내진성능에 대한 준정적 실험)

  • 정영수;한기훈;이강균;이대형
    • Journal of the Earthquake Engineering Society of Korea
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    • v.3 no.2
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    • pp.41-54
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    • 1999
  • Because of relatively heavy dead weight of concrete itself and unavoidable heat of massive concrete in bridge piers, circular hollow columns are widely used in Korean highway bridges. Since the occurrence of 1995 Kobe earthquake, there have been much concerns about seismic design for various infrastructures, inclusive of bridge structures. It is, however, understood that there are not much research works for nonlinear behavior of circular hollow columns subjected to eqrthquake motions. The objective of this experimental research is to investigate nonlinear behavior of circular hollow reinforced concrete bridge piers under the quasi-static cyclic load, and then to enhance their ductility by strengthening the plastic hinge region with glassfiber sheets. Particularly for this test, constant 10 cyclic loads have been repeatedly actuated to investigate the magnitude of strength degradation for the displacement ductility factor. Important test parameters are seismic design, confinement steel ratio, axial force and load pattern. It is observed from quasi-static tests for 7 bridge piers that the seismically designed columns and the retrofitted columns show better performance than the nonseismically designed colums, i.e. about 20% higher for energy dissipation capacity and about 70% higher for curvatures.

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Purification and Characterization of the Bacteriocin Produced by Lactococcus sp. KD 28 Isolated from Kimchi (김치에서 분리한 Lactococcus lactis가 생산하는 박테리오신의 정제 및 특성)

  • Lee, Ji-Young;Choi, Nack-Shick;Chun, Sung-Sik;Moon, Ja-Young;Kang, Dae-Ook
    • Journal of Life Science
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    • v.25 no.2
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    • pp.180-188
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    • 2015
  • The bacterial strain isolated from Kimchi showed antibacterial activity against Micrococcus luteus IAM 1056. The selected strain was identified as Lactococcus lactis by 16S rRNA nucleotide sequence analysis and named as Lactococcus sp. KD 28. The treatment of culture supernatant with proteinase K removed antibacterial activity, indicating its proteinaceous nature, a bacteriocin. This bacteriocin was sensitive to hydrolytic enzymes such as ${\alpha}$-chymotrypsion, trypsin, proteinase K, lipase, ${\alpha}$-amylase and subtilisin A. The bacteriocin was highly thermostable and resistant to heating at $80^{\circ}C$ for up to an hour but 50 % of the total activity was remained at $100^{\circ}C$ for 30 min. The pH range from 2.0 to 8.0 had no effect on bacteriocin activity and it was not affected by solvents such as acetonitrile, isopropanol, methanol, chloroform and acetone up to 50% concentration. The bacteriocin showed antibacterial activity against M. luteus IAM 1056, Lactobacillus delbrueckii subsp. lactis KCTC 1058, Enterococcus faecium KCTC 3095, Bacillus cereus KCTC 1013, B. subtilis KCTC 1023, Listeria ivanovii subsp. ivanovii KCTC 3444, Staphylococcus aureus subsp. aureus KCTC 1916, B. megaterium KCTC 1098 and B. sphaericus KCTC 1184. The bacteriocin was purified through ammonium sulfate concentration, SP-Sepharose chromatography and RP-HPLC. The molecular weight was estimated to be about 3.4 kDa by tricine-SDS-PAGE analysis.

Current Sensing Trench Gate Power MOSFET for Motor Driver Applications (모터구동 회로 응용을 위한 대전력 전류 센싱 트렌치 게이트 MOSFET)

  • Kim, Sang-Gi;Park, Hoon-Soo;Won, Jong-Il;Koo, Jin-Gun;Roh, Tae-Moon;Yang, Yil-Suk;Park, Jong-Moon
    • Journal of IKEEE
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    • v.20 no.3
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    • pp.220-225
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    • 2016
  • In this paer, low on-resistance and high-power trench gate MOSFET (Metal-Oxide-Silicon Field Effect Transistor) incorporating current sensing FET (Field Effect Transistor) is proposed and evaluated. The trench gate power MOSFET was fabricated with $0.6{\mu}m$ trench width and $3.0{\mu}m$ cell pitch. Compared with the main switching MOSFET, the on-chip current sensing FET has the same device structure and geometry. In order to improve cell density and device reliability, self-aligned trench etching and hydrogen annealing techniques were performed. Moreover, maintaining low threshold voltage and simultaneously improving gate oxide relialility, the stacked gate oxide structure combining thermal and CVD (chemical vapor deposition) oxides was adopted. The on-resistance and breakdown voltage of the high density trench gate device were evaluated $24m{\Omega}$ and 100 V, respectively. The measured current sensing ratio and it's variation depending on the gate voltage were approximately 70:1 and less than 5.6 %.