• Journal of Embryo Transfer
• /
• v.18 no.2
• /
• pp.85-90
• /
• 2003

This study examined the viability of canine oocytes following storage at 4 or 38$^{\circ}C$ for 5 h. Cumulus intact oocytes were collected from domestic dog following ovariohysterectomy at local veterinary clinics. In Exp I, the oocytes that collected from ovary transport different temperatures (4 or 38$^{\circ}C$) for 5 h, were cultured for (24 or 48 h). Survivability of oocytes judged by morphological appearance and PI (propidium iodide) staining. The survival rates at 4$^{\circ}C$ ovary transport group showed significantly lower than control group (0%; 0/129 vs. 72.9%; 129/177) 48 h after culture (P<0.05). In Exp II, to assess nuclear maturation of control group oocytes (ovary transported at 38$^{\circ}C$) after in vitro cultured for 24, 48 or 96 h. After 24 h and 48 h of culture, the metaphase I to metaphase II stages (MI to MII) was 8.3% (6/72) and 8.9% (9/101), and which was not increased at 96h (9.5%; 8/84). These results show that canine oocytes remarkably sensitive to low temperature and the percentage of oocytes reaching MI to MII did not increase 96 h after culture.

• Childhood Kidney Diseases
• /
• v.7 no.2
• /
• pp.174-180
• /
• 2003

Purpose : Unstable bladder has been known to be one of the reasons for the genesis and persistance of primary vesicoureteral reflux(VUR) in children. And treatment of unstable bladder by anticholinergic agent may contribute to the resolution of primary VUR. We evaluated the effect of an anticholinergic agent(oxybutynin) on the resolution of primary VUR in children with different toilet training and voiding functions. Methods : 152 children with persistant primary VUR after one year of follow up were randomly assigned to the oxybutynin group(n=59, oxybutynin 0.2 mg/kg twice daily) and the control group(n=93, no oxybutynin) at Ewha Womans University Mok-Dong Hospital from October 1996 to April 2002. The resolution rate of the VUR and the difference according to the status of toilet training and voiding dysfunction were analyzed. Statistical analysis was done by the Chi-square test and a P-value of less than 0.05 was considered as significant. Results : VUR was resolved in 49.2%, improved in 20.3% and not changed in 30.5% in the oxybutynin group(n=59) which was not significantly different to 45.2%, 16.1%, 38.7% in the control group(n=93), respectively. In the non-toilet trained young children, VUR was resolved in 50.0%, improved in 23.5% and not changed in 26.5% in the oxybutynin group(n=34) which was not significantly different to 44.2%, 19.2%, 36.6% in the control group(n=52), respectively. In the toilet trained older children, VUR was resolved in 48.0%, improved in 16.0% and not changed in 36.0% in the oxybutynin group(n=25) which was not significantly different to 46.3%, 12.2%, 41.5% in the control group(n=41), respectively. In the toilet trained older children with no voiding dysfunction, VUR was resolved in 33.3%, improved in 11.1% and not changed in 55.5% in the of oxybutynin group(n=9) which was not significantly different to 53.6 %, 10.7%, 35.7% in the control group(n=28), respectively. In the toilet trained older children with voiding dysfunction, VUR was resolved in 56.3%, improved in 18.7% and not changed in 25.0% in the oxybutynin group(n=16), which looked higher than 30.7%, 15.4%, 53.9% in the control group(n=13), respectively, but these were not significantly different either. Conclusion : Oxybutynin was not effective in the resolution of primary VUR in non-toilet trained young children and toilet trained older children. Oxybutynin showed slightly higher tendency of reflux resolution in toilet-trained older children with voiding dysfunction but the difference was not statistically significant. Judicious use of oxybutynin is required in selected older children with VUR and voiding dysfunction.

• Food Science and Preservation
• /
• v.6 no.3
• /
• pp.264-269
• /
• 1999

The effects of film thickness and moisture absorbing materials(MAM) on the ginger qualities were investigated during MA storage for 150 days. LDPE films of 0.04, 0.06 and 0.08mm thicknesses were applied to select the adequate film in gas permiability. MAMs of sheet and sachet type were applied to 0.06mm-LDPE film bag to prevent moisture condensation during storage. Thickness of film used affected neither weight loss nor firmness of gingers during U storage. But the thinner in thickness showed the less changes in sprouting, spoilage and reducing sugar. Moreover, compared with MAP alone, MAPs with MAM of both sachet and sheet styles led to the weight loss of gingers increasing by more than 3-4 times, but sprouting rate, spoilage rate and reducing sugar decreasing by 3.3, 1.4 and 2.3 times, respectively. These results showed that MAP with UM was significantly effective to prolong the freshness of ginger by longer than 2 times, compared with MAP alone.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.14 no.4
• /
• pp.381-386
• /
• 1985

This experiment was conducted to investigate changes of soybean curd yield according to the extension of soaking time during manufacturing of soybean curd. To investigate those changes systematically, transmission electron microscopy and disc-gel electrophoresis were used. The soybean curd yield was increased from 45.0% to 50.5% and 55.4% respectively as soaking time is extended from 5 hours to 10 and 24 hours. The solid extraction and soybean milk coagulation were also increased according to the extension of soaking time. From disc-gel electrophoresis patterns of soybean milk protein and soybean curd protein, numbers of band were increased and major band thickened by expending the soaking time. Most of high molecular bands of soybean milk protein were transfered to soybean curd. Crude 7S proteins of soybean milk and soybean curd in dis-gel electrophoresis were appeared to be 4 and 5 bands respectively, and crude 11S proteins of soybean milk and soybean curd were appeared to be 9 and 8 bands respectively. Of soybean milk bands, most of 11S component transfered to soybean curd. Transmission electron photomicrographs revealed that the dimension of each protein body became larger and the numbers of spherosome around the protein bodies in unit area fewer by extending the soaking time of soybean.

• Journal of Aquaculture
• /
• v.5 no.2
• /
• pp.177-182
• /
• 1992

Experiments were conducted with goldfish exposed to various levels of mercuric chloride(control group) and mercuric chloride with chitosan (experimental group). Dilutions of these two solutions were made in the concentration ranges 0.6 to 1.0 $mg/{\ell}$ and 1.2 to 2.0 $mg/{\ell}$, respectively. Fifty percent lethal concentration of mercuric chloride($LC_{50}$) for 48 hours with the species was between 0.6 and 0.7 $mg/{\ell}$. Exposure of goldfish to mercury produced a marked, dose-dependent mortality with elevation of concentration (P<0.05). However, at each concentration of mercuric chloride treated with chitosan, mortality decreased significantly compared to control group (P<0.05).

• Horticultural Science & Technology
• /
• v.33 no.5
• /
• pp.705-711
• /
• 2015

This study was conducted to maintain the postharvest shelf-life of summer strawberry cultivars 'Goha' and 'Flamengo' through $CO_2$ (90%) and $ClO_2$ gas ($5{\mu}L{\cdot}L^{-1}$) pre-treatment. The respiration rate of 'Goha' and 'Flamengo' treated with $CO_2$ and $CO_2$ + $ClO_2$ was the lowest during storage. The weight loss with $CO_2$ + $ClO_2$ treatment was about 1% lower than the control group and 'Goha' was the lowest during storage. The pre-treatments effect on soluble solids contents was minimized. The firmness with $CO_2$ and $CO_2$ + $ClO_2$ treatments was only about 30% higher than $ClO_2$ and control. The firmness of 'Goha' was about 2.15N and 'Flamengo' was 2.37N, 15 days after storage. Decayed fruit was shown from after 6 days after storage in control. Control group lost quality 10 days after storage due to decayed fruit rate 20% of 'Goha' and 'Flamengo'. $CO_2$ and $CO_2$ + $ClO_2$ treatments showed the lowest decay rate during storage. Especially, 'Goha' showed around 5% decay fruit rate 10 days after storage. As a result, $CO_2$ + $ClO_2$ pre-treatment is recommended to extend the shelf-life of the summer strawberries.

• Journal of Adhesion and Interface
• /
• v.3 no.2
• /
• pp.1-8
• /
• 2002

Polyurethane NCO prepolymers were synthesized with the polyols such as PTMG, GP and the isocyanate such as TDI at $40^{\circ}C$ for 8.5 minutes. As average molecular weights (${\bar{M_n}}$: 1000, 2000, 3000, 4000) of PTMG, and GP were decreased from 4000 to 1000, ratio of oil gelation increased from 298%, to 440%, for Bunker B. When oil and water were emulsified, the ratio of gelation was increased approximately two times. Ratio of gelation for emulsive Bunker B was increased from 402% to 910%, for PTMG1000 and increased from 440%, W 958% for GPI1000. Ratio of oil gelation for emulsive Bunk C which has higher viscosity than Bunker B was measured w 923% for PTMG1000 made with chain extender, i.e. EG, and measured to 1098% for GP1000. The gel made from GP which has three functional group showed soft and strong characteristic, as a result, it can be removed easily from oil spilled ocean.

• Korean journal of applied entomology
• /
• v.39 no.3
• /
• pp.193-197
• /
• 2000

Wolbachia, vertically transmitted bacterial endosymbionts, is known to induce cytoplasmic incompatibility (CI), parthenogenesis, or feminization in some insect species. The Wolbachia found to exist symbiotically as a non-virulent form in Drosophila melanogaster, however popcorn is a lethal strain of Wolbachia, which causes tissue degeneration and early death of its adult host. Popcorn-infected flies showed the delayed duration of an egg and larvae, and their pupal period and life span reduced. The oviposition and egg-hatching rate of popcorn-infected flies were decreased 15% and 80%, respectiv~ly, compared to those of the normal Wolbachia-infected flies. The pupation and emergence rates of popcorn-infected flies were 67% and 65%, respectively. When popcorn-infected flies were crossed with Wolba-chia-infected flies, and vice versa, both crosses resulted in a significant reduction in egg production, egg¬hatching, pupation, and emergence rate, and their progeny revealed the popcorn syndrome. When popcorn-infected male flies were crossed with uninfected females, popcorn was not detected in their progeny. Popcorn also maternally transmitted in flies, but it did not induce CI and affected on its host life cycle as a virulence.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2012.05a
• /
• pp.321-326
• /
• 2012

장기유출모형을 이용하여 농업용저수지 유입량을 예측하고 농업용수 필요수량 및 홍수기 저수지 홍수조절을 통한 방류량 데이터를 이용하여 장기간에 대한 농업용저수지 저수율을 계산하였다. 계산결과와 실측 저수율 데이터의 비교 검증을 통하여 모형의 적용성을 평가하였다. 대상유역은 담양댐 지점이며 유역면적은 $47.2km^2$ 이며 주 하천 연장은 12.0km 이다. 담양댐은 저수용량에 비하여 유역면적이 작기 때문에 댐 계획 당시 순창군 구림면에 유역면적 $18.4km^2$ 인 2개의 보를 축조하여 유역변경방식으로 간접유역 유출량을 비관개기 및 홍수시에 도수하며 최대 도수량은 $10m^3/s$이다. 장기유출모의는 한국수자원공사(2001)에서 수행한 전역최적화기법인 콤플렉스 혼합진화기법을 통하여 추정된 나주지점의 모형보정 성과를 활용하였으며 모의기간은 1981-2010년(30년)이다. 장기유출모의 결과 담양댐 유역의 평균 유출율은 67% 인 것으로 분석되었다. 농업용수 필요수량은 한국농촌공사에서 산정한 연도별 필요수량 산정결과를 이용하여 실측 농업용수 월별 방류량 자료를 기준으로 관개개간인 4월 21일-9월 20일(163일)동안 월별로 분배하여 적용하였다. 홍수조절은 기존 댐 상시만수위, 홍수기제한수위 데이터를 근거로 운영하였다. 일별저수지 운영모형은 미공병단의 HEC-5 모형을 이용하였으며 한국농어촌공사 농촌용수종합 정보시스템(RAMIS)의 댐 일별 저수율 현황과 기존저수지 일별 저수지 모의운영결과를 비교 검증하였다. 모형수행결과 실측저수율과 모형수행결과의 상관계수는 0.93 인 것으로 분석되었다. 연구결과, 장기유출모의 결과와 연계하여 농업용수, 하천유지용수, 홍수조절을 고려한 저수지 운영을 통하여 비교적 정확하게 농업용저수지 저수율을 예측할 수 있을 것으로 판단된다. 본 연구성과를 바탕으로 농업용저수지의 장기적인 용수수급현황을 예측하여 효율적인 용수공급계획을 수립할 수 있을 것으로 기대된다.

• Geophysics and Geophysical Exploration
• /
• v.25 no.2
• /
• pp.85-92
• /
• 2022

An elongated object in one direction can be approximated as a line segment. Here, the closed-form expressions of a line segment's vector magnetic and magnetic gradient tensor are required to interpret responses by a line segment. Therefore, the analytical expressions of the vector magnetic and magnetic gradient tensor are derived. The vector magnetic is converted from the existing gravity gradient tensor using Poisson's relation where the gravity gradient tensor caused by a line segment can be transformed into a vector magnetic. Then, the magnetic gradient tensor is derived by differentiating the vector magnetic with respect to each axis in the Cartesian coordinate system. The synthetic total magnetic data simulated by an iron pile on boreholes are inverted by a nonlinear inversion process so that the physical parameters of the iron pile, including the beginning point, the length, orientation, and magnetization vector are successfully estimated.