• KSCE Journal of Civil and Environmental Engineering Research
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• v.14 no.3
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• pp.523-534
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• 1994

The purpose of this study is to develop computer simulation model that produce precipitation patterns from stochastic model. In the paper(I) of this study, the alternate renewal process(ARP) is used for the daily precipitation series. In this paper(Il), stochastic simulation models for the daily precipitation series are developed by combining Markov chain for the precipitation occurrence process and continuous probability distribution for the precipitation amounts on the wet days. The precipitation occurrence is determined by first order Markov chain with two states(dry and wet). The amounts of precipitation, given that precipitation has occurred, are described by a Gamma, Pearson Type-III, Extremal Type-III, and 3 parameter Weibull distribution. Since the daily precipitation series shows seasonal variation, models are identified for each month of the year separately. To illustrate the application of the simulation models, daily precipitation data were taken from records at the seven locations of the Nakdong and Seomjin river basin. Simulated data were similar to actual data in terms of distribution for wet and dry spells, seasonal variability, and precipitation amounts.

• Journal of the Korea institute for structural maintenance and inspection
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• v.14 no.6
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• pp.238-245
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• 2010

Progressive collapse is defined as a collapse caused by sectional destruction of a structural member which links to other surrounding structures. Currently the design guidelines for the prevention of progressive collapse is not available in Korea. So, structural engineers have a difficulty in evaluating progressive collapse. In this study, the static and dynamic analysis to evaluate the methods and procedures are conducted using commercial analysis program for RC moment resisting frames. According to the study, DCR value of RC moment resisting frame system based on code in Korea is over 2 and it shows that it can't provide alternate load paths due to the progressive collapse. And additional reinforcement should be considered for the progressive collapse resistance. As a result of vertical deflection and DCR value of linear static analysis and linear dynamic analysis, the results of dynamic analysis were underestimated more than the result of static analysis. Thus, the dynamic coefficient value of 2 provides conservative estimation.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.9 no.3
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• pp.63-74
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• 1999

In this paper we present a Hangul Encryption Algorithm (HEA) which encrypts composition Hangul syllables into composition Hangul syllables using the non-linear structure of Hangul. Since ciphertexts generated by HEA are displayable characters HEA can be used in applications such as Privacy Enhanced mail (PEM) where ciphertexts should be displayable characters. HEA is based on DES and it can be shown that HEA is as safe as DES against the exhaustive key search differential cryptanalysis and linear cryptanalysis. HEA also has randomness of phonemes of ciphertexts and satisfies plaintext-ciphetext avalanche effect and key-ciphertext avalanche effect.

• Korean Journal of Microbiology
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• v.36 no.2
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• pp.142-149
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• 2000

It had been evaluated the recombinant Circumsporozoite(CS) protein of Plasmodium viva in serologic diagnosis of vivax malaria. Western blot was done to analyse the sera of malaria patients according to the days after onset. The sera which have the terms within 15 days were shown 43.8%(14/32) of positive rates and the sera over the 16 days were shown 94.4%(17/18) of positive rates. So the total positive rate was 62%(31/50). It was 22.6%(7/31) which was shown negative response in Western blot, even though they were shown positive response in Immuuofluorescent antibody test(1FAT) using whole blood stage antigens. The positive rate of non-epidemic area(Yechon-gun, Kyongsangbuk-do) was 10.7%(3/28), and epidemic area(Kangwha-gun, Inchon-shi) was 27.6%(13/47) in Western blot analysis using recombinant CS protein. In order to applicate the recombinant CS protein in seroepidemiological survey, blood samples of 422 inhabitants were collected who lived in malaria epidemic areas, Chosm-ri, Majeong-ri, Hyangyang-ri and Noejo-n in Paju-shi, Kyonggi-do. All of them were negative in microscopic examination and two(0.5%) of them were positive in Polymerase Chain Reaction. 42(10.0%) of them were seropositive in FAT using whole blood antigens and 71(16.8%) of them were seropositive in Enzyme-linked immunosorbent assay using recombinant CS protein. It was figured out the positive rates were much higher according to the distances of villages which were closed to the demilitalized zone(DMZ) in all kind of diagnostic methods, respectively.

• Journal of KIISE:Databases
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• v.34 no.6
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• pp.528-545
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• 2007

Recently ontology has been an attractive technology along with the business strategy of providing a plenty of more intelligent services. The essential problem in application domains using ontology is that all members, agents, and application programs in the domains must share the same ontology concepts. However, a variety of mobile devices, sensing devices, and network components manufactured by various companies, a variety of common carriers, and a variety of contents providers make multiple heterogeneous ontologies more likely to coexist. We can see many past researches fallen into resolving this semantic interoperability. Such methods can be broadly classified into by-mapping, by-merging, and by-translation. In this research, we focus on by-translation among them which uses a translation rule directly made between two heterogeneous ontology data like OntoMorph. However, the manual composition of the direct translation rule is not convenient by itself and if there are N ontologies, the direct method has the rule composition complexity of $O(N^2)$ in the worst case. Therefore, in this paper we introduce the cascade composition of translation rules based on web openness in order to improve the complexity. The research result made us recognize some important factors in an ontology translation system, that is speediness of translation, and conveniency of translation rule composition, and some experiments and comparing analysis with existing methods showed that our cascade method has more conveniency with insuring the speediness and the correctness.

• Parasites, Hosts and Diseases
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• v.35 no.2
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• pp.111-118
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• 1997

Four separate pairs of oligonucleotide primers within the coding region in a T sergenti 33-kDa surface protein gene were selected to detect T. sergenti by PCR. The specificity of PCR-amplified DNA was examined by digestion with restriction enzyme 3nd Southern blot hybridization using T. sergenti p33 DNA probe. PCR appears to be specific for T. sergenti, without detectable signals from uninfected erythrocytes, uninfected bovine leukocytes and other hemoparasites, including A. morginnle and 3. ouata. Although 46 of 71 specimens (64.8%) from grazing cattle were microscopically positive. PCR in this study showed that 64 specimens (88.7%) were positive. Therefore, PCR proves a useful diagnostic tool for detecting T sergenti-infected cattle. In addition, it is also revealed that PCR was significantly more sensitive than traditional microscopic examination using Giemsa's stain.

• Journal of fish pathology
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• v.19 no.3
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• pp.267-276
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• 2006

Seventy-five streptococci were isolated from diseased olive flounder, Paralichthys olivaceus in Jeju. Their drug susceptibility and transferable multiple drug resistance were characterized. All isolates were resistant to flumequine (AR) and oxolinic acid (OA) and 26 isolates (34.7%) showed 4～6 multiple resistance of ampicillin (ABPC), AR, doxycycline (DOXY), erythromycin (EM), norfloxacin(NOR), OA and oxytetracycline (OTC) in various combinations. pST9 of a transferable R plasmid was detected from a multiple drug resistance strain, Streptococcus sp., ST9 originated from diseased flounder in Jeju, previously. We performed DNA hybridization to know the distribution of plasmid with the same DNA structure as pST9 in streptococci. Thirteen out of 60 isolates analyzed were positive in colony DNA hybridization and the part of bacteria isolated from raw meal was also hybridized with pST9. It suggested that raw meal is one of the origin of the resistance plasmid and R plasmid with DNA structure differing from pST9 is also involving in multiple drug resistance of the streptococci. In conjugation experiment, we found transferable R plasmid carrying OTC, DOXY and/or EM resistance determinant in the 13 resistance strains. all of the streptococci carrying the transferable R plasmid were similar in RAPD patterns. However, pST -type R plasmid was rare in S. iniae most frequently appearing in flounder farm.

• Journal of Veterinary Clinics
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• v.28 no.4
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• pp.427-430
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• 2011

A 6-month-old intact female, domestic short hair cat was presented with dyspnea and anorexia for 2 days. Physical examination revealed muffled heart sound with labored breaths. Hyperproteinemia and hyperglobulinemia with polyclonal gammapathy was revealed. Pleural effusion was non-septic exudates, it also had hyperglobulinemia with decreased albumin: globuline ration. In addition, effusion RT-PCR for feline coronavirus was positive in this cat. Feline infectious peritonitis (FIP) was strongly suspected and aggressive treatments with human interferon-alpha, pentoxifylline, and glucocorticoids were initiated. The cat remained healthy without recurrence of pleural effusion during 5 months follow-up periods. To the author's knowledge, this is the first case report describing successful management of FIP with human interferon-alpha and pentoxifylline in Korea.

• Food Science and Preservation
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• v.15 no.1
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• pp.88-93
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• 2008

This paper focused on the detection of the genetically modified soybean (Glycine max L. MERRILL) samples to search for the speedy analysis methods. We have identified the PCR (polymerase chain reaction) assay with a newly developed technique called DHPLC (denaturing high performance liquid chromatography) to screen the GMO in soybean. The DHPLC is i1s ability to directly detection specific sequences of DNA by using column. With these characteristics. the DHPLC assay had the advantage of simplicity, rapidty could obtain result within 20 minutes. Whereas $15{\times}10^{-4}ng/{\mu}L$ concentration could be detected with the PCR analysis, $15{\times}10^{-5}ng/{\mu}L$ concentration could be detected with the DHPLC method. Therefore, DHPLC method was considered to be a simple, fast and sensitivity screening method rather than PCR analysis for GMO detection in soybean.

• Biomedical Science Letters
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• v.6 no.1
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• pp.73-82
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• 2000

Blunt-end DNA fragments can be inserted in two different orientations. Conventionally, their directions are determined by restriction enzyme digestion or by DNA sequencing, however, these methods are often limited in their use due to the lack of appropriate enzyme sites or large sample numbers, respectively. In the present study, a novel strategy and the corresponding protocol for the simple determination of insert orientation is introduced. Using conventional sequencing primers and PCR primers that have been used for amplification of the insert, single clones, which have inserted the fragment in the desired orientation, were easily identified by this PCR-based method. The fidelity of this system was confirmed by cloning of a tar urocortin cDNA, which is a recently discovered neuropeptide. Recombinant clones identified by this method were further shown to be fully functional, and using these, for the first time, urocortin was recombinantly expressed in eukaryotic cells.