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The Detection of Genetically Modified Organisms in Soybean by DHPLC and Polymerase Chain Reaction  

Lee, Kyoung-Hae (Department of Food Science & Biotechnology, Dongnam Health College)
Park, Su-Min (GenetBio, Chungnam Animal Science Center, Konyang Univ.)
Publication Information
Food Science and Preservation / v.15, no.1, 2008 , pp. 88-93 More about this Journal
Abstract
This paper focused on the detection of the genetically modified soybean (Glycine max L. MERRILL) samples to search for the speedy analysis methods. We have identified the PCR (polymerase chain reaction) assay with a newly developed technique called DHPLC (denaturing high performance liquid chromatography) to screen the GMO in soybean. The DHPLC is i1s ability to directly detection specific sequences of DNA by using column. With these characteristics. the DHPLC assay had the advantage of simplicity, rapidty could obtain result within 20 minutes. Whereas $15{\times}10^{-4}ng/{\mu}L$ concentration could be detected with the PCR analysis, $15{\times}10^{-5}ng/{\mu}L$ concentration could be detected with the DHPLC method. Therefore, DHPLC method was considered to be a simple, fast and sensitivity screening method rather than PCR analysis for GMO detection in soybean.
Keywords
Soybean; GMO; PCR; DHPLC;
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Times Cited By KSCI : 3  (Citation Analysis)
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1 Shin, W.S. and Kim, M. (2003) Optimized condition of genomic DNA eatraction and PCR methods for GMO detection in potato, Korean J. Food Sci. Technol., 34, 591-597
2 Roberts, P.S., Jozwiak, S., Kwiatowski, D.J. and Dabora, S.L. (2001) Denaturing high performance liquid chromatography (DHPLC) is a highly sensitive, semi- automated method for identifying mutations in the TSCI gene. J. Biochem. Biophys. Meth., 47, 33-38   DOI   ScienceOn
3 Lin, H.Y., Chiueh, L.C. and Shih, Y.C. (2000) Detection of genetically modified soy beans and maize by the polymerase chain reaction method. J. Food Drug Analysis, 8, 200-207
4 KFDA. (2000) Biotech labelling standards for processed foods. Kore Food and Drug Administration, No. 2000-43, Seoul, Korea
5 Lipp, M., Bluth, A., Eyquem, F., Kruse, L., Schimmel, H., Eede, G.V., et al. (2001) : Validation of a method based on polymerase chain reaction for the detection of genetically modified organisms in various processed foodstuffs. Eur. Food Res. Technol., 212(4), 497-504   DOI
6 Matsuoka, T., Kuribara, H., Takubo, K., Akiyama, H., Muira, H., Goda, Y., et al. (2002) Detection recombinant DNA segments introduced to genetically modified maize(Zea mays). J. Agri. Food Chem., 50, 2100-2109   DOI   ScienceOn
7 Meyer, R. (1999) Development and application of DNA analytical methods for the detection of GMOs in food. Food Control, 10, 391-399   DOI   ScienceOn
8 Birch, L., Charlotte, L., Archard, Helen, C., Parkes, David, G., et al (2001) Evaluation of LabChipTM technology for GMO analysis in food. Food Control, 12, 535-540   DOI   ScienceOn
9 Muscarella, L.A., Piemontese, M.R., Barbano, R., Fazio, A., Guarnieri and Guarnieri, V. et al (2007) Novel mutations of dystrophin gene in DMD patients detected by rapid scanning in biplex exons DHPLC analysis. Biomol. Eng., 24, 231-236   DOI   ScienceOn
10 Lipp, M., Anklam, E. and Stave, J.W. (2000) Validation of an immunoassay for detection and quantification of a genetically modified soybean in food and food fractions using reference materials: interlaboratory study. JAOAC., 83, 919-927
11 Meyer, R., Chardonnens, F. Hűber, P. and Luthy, J. (1996) Polymerase chain reaction (PCR) in the qualityand safety assurance of food : detection of soya in processed meat products. Z. Lebnsm. Unters. Forsch. 203, 330-344
12 Oefner, P.J. (2000) Allelic discrimination by denaturing high performance liquid chromatography. J. Chromatogr. Biomed. Sci., 739, 345-355   DOI   ScienceOn
13 Lin, H.Y., Chiang, J.W. and Shih, Y.C. (2001) Detection of genetically modified soy beans by PCR method and immunoassay kit. J. Food Drug Analysis, 9, 160-166
14 Tengel, C., SchuBler, P., Setzkw, W., Balles, J. and Sprenger-HauBles, M. (2001) PCR-based detection of genetically modified soybean and maizein raw and highly processed foodstuffs. Biotechniques, 31, 426-429
15 Lipp, M., Brodmann, K., Pietsch, K., Pauwels, J. and Anklam, E. (1999) IUPAC collaborative trial study of a method to detect genetically modified soy beans and maize in dried powder. JAOAC., 82, 923-928
16 Shin, W.S. and Kim, M. (2003) Optimized condition of genomic DNA extraction and PCR methods for GMO cetection in potato. Korean J. Food Sci. Technol., 35, 591-597   과학기술학회마을
17 Kim, M.H., Kim, J.W., Chae, K.Y., Park, S.W., Kim, Y.S. and Kyung, K.H. (2002) Consumer awareness and perception of genetically-modified foods in Korea; 2000-2001. J. Food Hyg. Saf., 17, 157-165   과학기술학회마을
18 Kim, M.H., Ahn, J.M., Park, S.W., Kim, Y.S. and Kung, K.H. (2001) Survey of consumer awareness and attutudes about food biotechnology on Korean, J. Food Hyg. Saf., 16, 152-158   과학기술학회마을
19 Hubner, P., Studer, E. and Luthy, J. (1999) Quantitative competitive PCR for the detection of genetically modified organisms in food. Food Control, 10, 353-358   DOI   ScienceOn
20 KFDA. (2001) Biotech labelling standards for processed foods(revised). Kore Food and Drug Administration, Seoul, Korea, No. 2001-43
21 Mathews, C. K., & Holder, K. E. V. (1990) Part II: Molecular architecture of living matter. C. K. Mathews & K. E. V. Holder (Eds.), Biochemistry p.146-148
22 James, C. (2001) Global review of commercialized transgenic crops; 2001, ISAAA briefs Ithaca, NY, No. 24, p.1-31
23 Meyer, R., Chardonnens, F., Hubner, P. and Luthy, J. (1996) Polymerase chain reaction (PCR) in the quality and safety assurance of food: detection of soya in processed meat products. Eur. Food Res. Technol., 203(4), 339-344
24 Goldenberg, O., Herrmann, S., Marjoram, G., Noyer- Weidner, M. and Hong, G., et al (2007) Molecular monitoring of the intestinal flora by denaturing high performance liquid chromatography. J. Microbiol. Methods, 68, 94-105   DOI   ScienceOn