• 제목/요약/키워드: 연쇄

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Development of Detachable PDMS/Glass PCR-Chip and It's Application to Detection of Male Infertility (분리식 PDMS/유리 중합효소연쇄반응칩 개발 및 유전적 남성불임 검사에의 응용)

  • Ju, Jin-Kyoung;Hwang, Seung-Young;Ahn, Yoo-Min
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.32 no.4
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    • pp.371-377
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    • 2008
  • Our precedent study has reported glass-PDMS (polydimethylsiloxane) based biochip for the gene PCR (polymerase chain reaction). To prevent the contamination of bio sample, the once used biochip must not be used repeatedly. However, the fabrication cost of microheater and microsensor of the biochip was not cheap to use it as a disposable chip. This paper proposes new PCR-chip where the glass substrate integrated with the microheater and microsensor is detachable from the reaction chamber where the sample is injected. That makes it possible to reuse the glass substrate repeatedly. The performance of the proposed detachable PCR-chip was compared with that of the precedent monolithic PCR-chip. The results showed that the SRY (sex determining Y chromosome) gene PCR was successfully performed in the detachable chip compared with the monolithic chip. However, the more efforts to improve the efficiency of surface treatment of PDMS chip are needed to increase the possibility of applying the detachable chip to the detecting of male infertility.

Encounter Measure System Against Cyber-Terror And Legalism (사이버테러 대응체제와 법치주의)

  • Jeong, Jun-hyeon;Kim, Kui-nahm
    • Convergence Security Journal
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    • v.4 no.3
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    • pp.83-90
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    • 2004
  • Preventive measures and control over cyber terrorism in Korea is a complex problem. Today laws should meet requirements made by modern technologies development, Law enforcement, special services and judicial system cooperation, their efforts coordination and their material security are priority directions, None of the country is able to prevent cyber terror independently and international cooperation in this field is vital. Taking the above into consideration, we propose and inisit that National Intelligence Service(NIS) should share cyber terror data with Police Agency and have top police authority over the cyber terror.

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Logistics Strategy of Promotion for the B2B EC (기업간 전자상거래 활성화를 위한 물류혁신전략)

  • Lee, Kwang-Beom
    • International Commerce and Information Review
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    • v.3 no.1
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    • pp.197-223
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    • 2001
  • 최근 B2B 전자상거래 확산에 따른 물류문제를 해결하고 오프라인 물류와의 차별화롤 시도하기 위한 목적으로 다양한 물류측면의 대안이 제시되고 있다. 먼저 물류산업 자체의 e-business화를 추진함으로 기존 물류체계에서의 고비용 문제, 책임 소재가 불분명한 서비스 문제를 해결하고 운송정보의 실시간 제공을 통한 발주자, 화주자 간의 긴밀한 커뮤니케이션 형성과 해외시장을 겨냥한 글로벌 물류체계를 구축해야 한다. 다음으로 물류업종의 지나친 세분화, 기존 물류업체의 영세성과 혁신마인드의 부족, 그에 따른 제조 유통업체의 경쟁적 자가물류 확충의 악순환으로 제3자 물류시장 성장기반이 제한되어 오는 등 구조적인 문제점을 극복하기 위하여 주요 운송업체가 영세한 화주나 창고관리 위탁업무까지 대행하여 주는 지금까지의 제3자 물류 체제에서 대기업이 물류시장에 새롭게 진출하면서 사이버 물류시스템을 기반으로 기존 주요 운송업계와 VAN 사업자가 장악하고 있는 물류정보 서비스를 주도하는 제4자 물류체제로의 전환이 요구된다. 왜냐하면, SCM과 전자상거래 환경 하에서는 공급 망 내의 물류를 최적화하여 기업의 경쟁력을 강화하기 위해서 기능적 아웃소싱의 단계를 거쳐 물류기능의 통합과 운영의 자율권이 증대, 전체적인 공급연쇄 솔루션을 제공하는 서비스 제공자와 함께 기업의 경영자원, 능력, 기술을 관리하고 결합하는 공급연쇄 통합자로서 제4자 물류가 필요하기 때문이다. 이와 같은 제4자 물류체제가 본격 형성될 경우 물류정보와 인프라가 복잡하게 업혀있는 기존 물류산업이 재편되고, 특화된 물류솔루션을 바탕으로 한 산업별 물류체제 구축이 가속화될 것으로 예견되며, 일반기업 입장에서 물류부문의 아웃소싱이 촉진되어 물류산업기반이 확충될 수 있을 것이다.

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A TDMA-based MAC protocol in hybrid-vehicular communication systems for preventing a chain-reaction collision on a highway (하이브리드 차량 통신 시스템에서 연쇄 추돌 사고 방지를 위한 TDMA 기반 MAC 프로토콜)

  • Oh, Sang-Yeob
    • Journal of Digital Convergence
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    • v.10 no.8
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    • pp.179-184
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    • 2012
  • A car accident on a highway occurs a chain-reaction collision because of a vehicle's fast velocity. In order to prevent it, the accident vehicle should broadcast a safe message to its neighbors. If there are many neighbor nodes, a frame collision probability is high. To solve this, it was proposed for a system as a previous study to send a safe message without frame-collision using separating channels. However, the separation of multiple channels make feasibility low because of increasing hardware's development cost and complexity. In this paper, we proposes a TDMA-based MAC protocol using a single channel. As a result, we show the frame reception success rate of our protocol was almost the same as the previous protocol.

A Study on the Validation system of Detection for Biological Agents Using Real-Time PCR (실시간 중합효소 연쇄반응을 활용한 생물작용제 검증시스템 연구)

  • Cha, Younggil;Koo, Bonwoo;Kim, Seongjoo;Kim, Namil;Park, Hanoh
    • Journal of the Korea Institute of Military Science and Technology
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    • v.20 no.5
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    • pp.726-732
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    • 2017
  • Bacillus anthracis, Vibrio cholerae, Variola virus and Shigella dysenteriae are classified as category A and B biological weapons. In this study suggest that 4 genes of Bacillus anthracis, 2 genes of Vibrio cholerae, 1 gene of Variola virus and 1 gene of Shigella dysenteriae were detective 50~500 fg of target DNA per reaction using real-time PCR based assay. Also analytical specificity did not show any cross-reactivity with other related bacteria. Reliable and one reaction could be effective early diagnostic and treatment for detection of unknown samples.

Chemical Structure Study on Copolyterephthalates Based on Ethylene Glycol and 1, 4-Cyclohexane Dimethanol by High Resolution NMR Analysis (고분해능 NMR 분석법에 의한 에틸렌글리콜과 1, 4-시클로헥산디메탄올의 테레프탈산 공중합체의 화학구조 연구)

  • Yoo, Hee-Yeoul;Kim, Sang-Wook;Okui, Norimasa
    • Applied Chemistry for Engineering
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    • v.4 no.4
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    • pp.770-775
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    • 1993
  • Chemical structure of poly(ethylene terephthalate-co-1, 4-cyclohexylene dimethylene terephthalate), P(ET-CT) copolyesters was investigated by High Resolution NMR analysis. The copolymer composition and isomeric ratio were determined by methylene resonance peaks which were separated into three peaks corresponding to ET, trans CT and cis CT units, respectively. The copolymer sequence distribution was evaluated from the carbon resonance peaks connected to carbonyl groups in benzene, indicating died distribution. According to statistics model, these copolyesters are almost random copolymers. The copolymer sequence distribution could be simulated and its averaged length was calculated by random copolymer statistics.

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Climate Change Impacts on Meteorological Drought and Flood (기후변화가 기상학적 가뭄과 홍수에 미치는 영향)

  • Lee, Dong-Ryul;Kim, Ung-Tae;Yoo, Chul-Sang
    • Journal of Korea Water Resources Association
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    • v.37 no.4
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    • pp.315-328
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    • 2004
  • Recent increase of green house gases may increase the frequency of meteorological extremes. In this study, using the index and meteorological data generated by the Markov chain model under the condition of GCM predictions, the possible width of variability of flood and drought occurrences were predicted. As results, we could find that the frequency of both floods and droughts would be increased to make the water resources planning and management more difficult. Thus, it is recommended to include the effect of climate change on water resources in the related policy making.

Prediction in run-off triangle using Bayesian linear model (삼각분할표 자료에서 베이지안 모형을 이용한 예측)

  • Lee, Ju-Mi;Lim, Jo-Han;Hahn, Kyu-S.;Lee, Kyeong-Eun
    • Journal of the Korean Data and Information Science Society
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    • v.20 no.2
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    • pp.411-423
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    • 2009
  • In the current paper, by extending Verall (1990)'s work, we propose a new Bayesian model for analyzing run-off triangle data. While Verall's (1990) work only account for the calendar year and evolvement time effects, our model further accounts for the "absolute time" effects. We also suggest a Markov Chain Monte Carlo method that can be used for estimating the proposed model. We apply our proposed method to analyzing three empirical examples. The results demonstrate that our method significantly reduces prediction error when compared with the existing methods.

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