• Title/Summary/Keyword: 연쇄

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The Rapid Diagnosis of $\beta$-Haemolitic Streptococcus sp. by Immunoperoxidase Method (면역효소항체법에 의한 $\beta$-용혈성 연쇄구균 Streptococcus sp.의 신속진단에 대하여)

  • Chun, Seh-Kyu;Choi, Dong-Lim;Park, Soo-Il
    • Journal of fish pathology
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    • v.1 no.2
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    • pp.103-110
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    • 1988
  • For the rapid diagnosis of bacterial diseases of cultured fishes, the immunoperoxidase method was applied to the detection of $\beta$-haemolitic Streptococcus sp. strain KST-2 isolated from tilapia(Oreochromis niloticus). The suitability of field analysis and the sensitivity of the immunoperoxidase method was compared with those of the counterimmunoelectrophoresis(CIE) and the immunodiffusion(ID). Results of testing cross-reactivity which did not indicate any cross reactivity with other fish pathogens, this method was specific to Streptococcus sp. The sensitivity of this method was $1{\times}10^3CFU/ml$ which was at least $10^2$times greater than the CIE and $10^4$times greater than the ID. The immunoperoxidase method was more suitable for field application and more sensitive than other diagnostic techniques tested on this study.

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Relationship between Diurnal Patterns of Passenger Ridership and Passenger Trip Chains on the Metropolitan Seoul Metro System (수도권 광역도시철도 하루 시간대별 이용 빈도에 의해 구분된 역 집단과 통행자의 통행 연쇄 패턴 간 관계)

  • Lee, Keum-Sook;Park, Jong-Sook;Kim, Ho-Sung;Joh, Chang-Hyeon
    • Journal of the Korean Geographical Society
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    • v.45 no.5
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    • pp.592-608
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    • 2010
  • This study investigates the diurnal pattern of transit ridership in the Metropolitan Seoul area. For the purpose, we use a weekday Smart Card passenger transaction data in 2005. Eleven passenger trip patterns are found from 2.74 million passengers moving on the Metropolitan Seoul Metro system. Among them, we analyze 2.4 million passengers blonging to five trip types having only one or two transaction record during a day. A total of 357 metro stations are classified to four types according to their diurnal pattern of passenger riderships. We analyze the relationships between passenger's trip chain patterns and subway station's diurnal transit ridership patterns. The result shows that the ratio of the number of passengers of particular time of the day is hierarchically related with trip chain patterns.

Performances of wireless ATM cell transmission with partial concatenated coding (무선 ATM셀 전송을 위한 부분 연쇄 부호화 기법의 성능분석)

  • 이진호;김태중;이동도;안재영;황금찬
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.22 no.9
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    • pp.2014-2026
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    • 1997
  • In this paper, the performances of wireless asynchronous transfer mode (ATM) cell transmission in mobile work are analyzed. We adopt 16Star QAM as amodulation technique in wireless channel and considered Reed-Solomon, convolutional, and concatenated coding to improve the error rate performances, and also proposed the Partial Concatenated Coding (PCC) technique as UEP(unequal error protection) code for efficient transmission of ATM cell in the air interface. We consider Doppler's effect, Rician fading, and diversity technique of maximal-ratio combining (MRC) for mobile channel model. For performance measure, we analyze bit error rate, ATM cell loss probability, ATM cell error probability, and network performances of ATM cell transmission delay and throughput. The numerical results show that the adoption of PCC is a prospective way for the evolution of future wireless ATM network on mobile environment.

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Progressive Collapse Resisting Capacity of Braced Frames (가새골조의 연쇄붕괴 저항성능)

  • Kim, Jin-Koo;Lee, Young-Ho;Choi, Hyun-Hoon
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.21 no.5
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    • pp.429-437
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    • 2008
  • In this study the progressive collapse potential of braced frames were investigated using the nonlinear static and dynamic analyses. All of nine different brace types were considered along with a special moment-resisting frame for comparison. According to the pushdown analysis results, most braced frames designed per current design codes satisfied the design guidelines for progressive collapse initiated by loss of a first story mid-column; however most model structures showed brittle failure mode. This was caused by buckling of columns after compressive braces buckled. Among the braced frames considered, the inverted- V type braced frames showed superior ductile behavior during progressive collapse. The nonlinear dynamic analysis results showed that all the braced frame model structures remained in stable condition after sudden removal of a column, and their deflections were less than that of the moment-resisting frame.

Sensitivity Analysis of Steel Frames Subjected to Progressive Collapse (철골조의 연쇄붕괴 민감도 해석)

  • Park, Jun-Hei;Kim, Jin-Koo;Lee, Tae-Hyung
    • Journal of the Computational Structural Engineering Institute of Korea
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    • v.21 no.3
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    • pp.211-216
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    • 2008
  • Recently a lot of researches have been conducted on the progressive collapse of structures which is the total collapse of structures initiated by localized damage. Most of the previous studies on the field of progressive collapse have followed deterministic approach without considering uncertainty involved in design variables, which results in unknown reliability of the analysis results. In this study the sensitivity analyses are carried out with design variables such as yield strength, live load, damping ratio, and elastic modulus on the vertical deflection of the joint from which a column is suddenly removed. The Monte Calro simulation, tornado diagram method, and the first order second moment method(FOSM) are applied for the sensitivity study. According to the nonlinear static analysis results, the vertical deflection is most affected by the variation of yield strength of beams. The nonlinear dynamic analyses show that the behaviour of model structures is highly sensitive to variation of the yield strength of beams and the structural damping ratio.

Diagnostic testing for Duchenne/Becker Muscular dystrophy using Dual Priming Oligonucleotide (DPO) system (Dual Priming Oligonucleotide (DPO) system을 이용한 듀시엔/베커형 근이영양증 진단법)

  • Kim, Joo-Hyun;Kim, Gu-Hwan;Lee, Jin-Joo;Lee, Dae-Hoon;Kim, Jong-Kee;Yoo, Han-Wook
    • Journal of Genetic Medicine
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    • v.5 no.1
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    • pp.15-20
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    • 2008
  • Purpose : Large exon deletions in the DMD gene are found in about 60% of DMD/BMD patients. Multiplex PCR has been employed to detect the deletion mutation, which frequently generates noise PCR products due to the presence of multiple primers in a single reaction as well as the stringency of PCR conditions. This often leads to a false-negative or false-positive result. To address this problematic issue, we introduced the dual primer oligonucleotide (DPO) system. DPO contains two separate priming regions joined by a polydeoxyinosine linker that results in high PCR specificity even under suboptimal PCR conditions. Methods : We tested 50 healthy male controls, 50 patients with deletion mutation as deletion-positive patient controls, and 20 patients with no deletions as deletion-negative patient controls using DPO-multiplex PCR. Both the presence and extent of deletion were verified by simplex PCR spanning the promoter region (PM) and 18 exons including exons 3, 4, 6, 8, 12, 13, 17, 19, 43-48, 50-52, and 60 in all 120 controls. Results : DPO-multiplex PCR showed 100% sensitivity and specificity for the detection a deletion. However, it showed 97.1% sensitivity and 100% specificity for determining the extent of deletions. Conclusion : The DPO-multiplex PCR method is a useful molecular test to detect large deletions of DMD for the diagnosis of patients with DMD/BMD because it is easy to perform, fast, and cost-effective and has excellent sensitivity and specificity.

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Clinical Manifestations of Invasive Infections due to Streptococcus pyogenes in Children (소아에서 발생한 A군 연쇄구균에 의한 침습성 질환의 임상적 특성 분석)

  • Yang, Nuri;Lee, Hyeon Seung;Choi, Jae Hong;Cho, Eun Young;Choi, Eun Hwa;Lee, Hoan Jong;Lee, Hyunju
    • Pediatric Infection and Vaccine
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    • v.21 no.2
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    • pp.129-138
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    • 2014
  • Purpose: Streptococcus pyogenes is an important cause of invasive diseases in children. We aimed to describe the clinical characteristics of invasive infections due to S. pyogenes in children in Korea. Methods: A retrospective study of children under 18 years of age with invasive infections due to S. pyogenes at Seoul National University Children's Hospital between March 1992 and December 2012, and Seoul National University Bundang Hospital between March 2003 and December 2012 was conducted. Demographic factors, clinical characteristics, laboratory findings, treatment, mortality and morbidity of all patients were reviewed. Results: A total of 30 among 36 cases identified as invasive disease due to S. pyogenes were available for review. There was a predominance for male subjects (male:female=2.75:1). The median age was 50 months (range 12 days to 15 years) and 53.3% were under 5 years of age. Skin and soft tissue infections (9/30, 30.0%), bacteremia without identified focus (4/30, 13.3%) and bone and joint infections (6/30, 20.0%) were the most frequent clinical presentations. Streptococcal toxic shock syndrome (3/30, 10.0%) pulmonary, abdomen and central nervous system infections (2/30, 6.7%) were also seen. There was a peak in number of patients in year 2012 (9/30, 30.0%). There were no cases of mortality. Erythromycin and clindamycin resistance rates were low by 3.8% and 7.5%, respectively. Conclusion: We studied the clinical presentations of invasive infections due to S. pyogenes during the past 20 years in Korean children. The findings of this study help us understand the characteristics of the disease, enhancing early recognition and prompting adequate antibiotic therapy which is important in reducing morbidity and mortality.

A Study on the Distribution of Mycoplasma pneumoniae Infectious Disease in Children from 2016 to 2018 According to Age (2016년에서 2018년의 소아 마이코플라즈마폐렴균 감염질환의 연령별 발생분포에 대한 연구)

  • Junhyung Choi;Ki Hwan Kim
    • Pediatric Infection and Vaccine
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    • v.30 no.3
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    • pp.121-128
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    • 2023
  • Purpose: This study aimed to investigate the recent age distribution of Mycoplasma pneumoniae in patients with respiratory infections and the differences in diagnostic usefulness according to the methods used in these patients. Methods: We retrospectively reviewed the medical records of patients aged ≤18 years with respiratory infectious diseases who underwent polymerase chain reaction (PCR) or a specific immunoglobulin M (IgM) test between July 2016 and February 2019. The diagnosis of M. pneumoniae infection was confirmed by a positive result in the PCR or IgM test. Results: Of the 2,721 patients tested for M. pneumoniae, 2,197 underwent IgM, and 1,144 underwent PCR, with positivity rates of 17% and 20%, respectively. Among the 620 patients tested for both IgM and PCR tests simultaneously, 35% had M. pneumoniae infection, with 14% under 1 year old and 13% under 1-2 years old. The positive rate increased with age in both tests. Higher positive rates were observed in the IgM test before 3 years of age and in the PCR test after 3 years of age. The agreement rate between the two tests was 77.9% (Cohen's kappa 0.402). Conclusions: As age increased, the rates of M. pneumoniae infection also increased. In patients under 2 years of age, 4¬-14% of infections were confirmed depending on the method used. The moderate agreement between the PCR and IgM tests suggests that the simultaneous use of PCR and the IgM test for the early diagnosis should be approached with caution.

Distribution of Indicator Organisms and Influence of Storage Temperature and Period in Commercial Animal Foods (시판 동물성 식품의 오염지표세균 분포 및 저장온도, 기간별 오염지표세균의 변화)

  • 이용욱;박석기
    • Journal of Food Hygiene and Safety
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    • v.13 no.4
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    • pp.430-440
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    • 1998
  • The average number of total viable counts for the commercial pork tested was 19/g, coliform 1.8/g, psychrophilic bacteria 15/g, heterotrophic bacteria 12/g, fecal streptococcus 6.2/100 g, Pseudomonas aeruginosa 13/100 g and none of heat-resistant bacteria and Staphylococcus was detected. That for the commercial beef tested was 130/g, coliform 5.2/g, psychrophile 140/g, heterotroph 28/g, Staphylococcus 1.2/g, fecal streptococcus 9.5/100 g, Pseud. aeruginosa 1.9/100 g and heat-resistant bacteria was not detected. That for the commercial chicken tested was 8800/g, coliform 53/g, psychrophile 4600/g, heterotroph 4700/g, fecal streptococcus 9.9/100 g, Pseudo aeruginosa 2.5/100 g. That for milk was 4700/ml, psychrophile 120/ml, heterotroph 420/ml and the others were not detected. That for the commercial cheese was 3.2/g, psychrophile 2.3/g, heterotroph 1.6/g, Staphylococcus l/g, fecal streptococcus 9.1/g. That for fermented milk was $10^{7}/ml$, heatresistant bacteria $10^{6}/ml$, fecal streptococcus 2400/100 ml, lactobacillus $3.2{\times}10^{15}/ml$, in accordance with lactic acid bacteria and the others were not detected. There was not detected any indicator organisms from ham, sausage, butter, eggs and quails in the commercial fooods tested. SPC, coliform, psychrophile and heterotroph in commercial meats stored at $10^{\circ}C$ were increased rapidly as time goes on but heat-resistant bacteria, staphylococcus, fecal streptococcus and Pseudo aeruginosa were constant. At $20^{\circ}C$, SPC, coliform, psychrophile, heterotroph and fecal streptococcus were the highest at 7 days and heat-resistant bacteria, staphylococcus and Pseudo aeruginosa were increased a little. At $30^{\circ}C$, all indicators were increased rapidly for 3 and 7 days and then decreased rapidly. All indicator organisms were increased at the level of 10/g for 14 days in meat products stored at $10^{\circ}C$, but SPC, psychrophile and heterotroph in meat products stored at $20^{\circ}C$ were increased at the level of $lO^5/g$. It showed that the indicators in meat products stored at $30^{\circ}C$ had a tendency to increase at the level of $10^{2}/g$ relative to those stored at $20^{\circ}C$. SPC, psychrophile and heterotroph in milk stored at $10^{\circ}C$ increased up to the level of $10^4/ml$, but coliform, staphylococcus, fecal streptococcus and Pseudo aeruginosa were not detected. As stored at $20^{\circ}C$ and $30^{\circ}C$, they were increased rapidly for 1 or 3 days and then constant for a long time.

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Towards Real-time Multi-object Tracking in CPU Environment (CPU 환경에서의 실시간 동작을 위한 딥러닝 기반 다중 객체 추적 시스템)

  • Kim, Kyung Hun;Heo, Jun Ho;Kang, Suk-Ju
    • Journal of Broadcast Engineering
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    • v.25 no.2
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    • pp.192-199
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    • 2020
  • Recently, the utilization of the object tracking algorithm based on the deep learning model is increasing. A system for tracking multiple objects in an image is typically composed of a chain form of an object detection algorithm and an object tracking algorithm. However, chain-type systems composed of several modules require a high performance computing environment and have limitations in their application to actual applications. In this paper, we propose a method that enables real-time operation in low-performance computing environment by adjusting the computational process of object detection module in the object detection-tracking chain type system.