• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.20 no.2 s.33
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• pp.89-101
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• 2007

Objectives : This study was carried out to investigate the effects of Jeondo-San(JDS) on anti-Inflammation and anti-Propionibacterium acnes. Methods : The effects of JDS on anti-Inflammation and anti-Propionibacterium acnes were measured by the cytotoxicity of Raw 264.7 cell, the inhibition for NO, $TNF-{\alpha}$, $PGE_2$, iNOS and COX-2, the blocking $NF-{\kappa}B$ into nucleus and the sterilizing power for Propionibacterium acnes. Results : 1. All concentrations of JDS has no cytotoxicity in Raw 264.7 cell. 2. All concentrations of JDS inhibited the production of NO in the Raw 264.7 cell stimulated with LPS. 3. All concentrations of JDS did not significantly inhibit the production of $TNF-{\alpha}$ in the Raw 264.7 cell stimulated with LPS. 4. All concentrations of JDS inhibited the production of $PGE_2$ in the Raw 264.7 cell stimulated with LPS. 5. All concentrations of JDS did not inhibit the expression of COX-2 but concentrations of 50\;{\mu}g/ml$, 100\;{\mu}g/ml$ JDS inhibited iNOS expression in the Raw 264.7 cell stimulated with LPS. 6. Concentrations of 50\;{\mu}g/ml$, 100\;{\mu}g/ml$ JDS has the effect of blocking $NF-{\kappa}B$ into nucleus in LPS-induced macrophage Raw 264.7 cell. 7. All concentrations of IDS did not have the inhibitory effect of Propionibactrium acnes. Conclusions : The present date suggest that JDS has a effect on the stage of inflammation of acne.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.28 no.2
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• pp.23-32
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• 2015

Objective : The purpose of this study was to develop a Pattern Diagnosis Instrument of Acne in traditional Korean Medicine. Methods : Data was collected by structured survey papers from 20 professors of The Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology Society(Delphi method). We investigated importance rates of the symptoms of each Pattern in the survey papers, then calculated Weighted Value of the symptoms. Results : Nine objective symptoms and nine subjective symptoms were selected by investigated importance rate in the survey from 20 professors of The Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology Society. In Wind-Heat pattern(WHP, 風熱型), inflammatory lesion of acne was the most important symptom. Oily skin, nodule and effect of menstruation are the most important symptoms in Dampness-Heat pattern(DHP, 濕熱型), Phlegm-Stasis pattern(PSP, 痰瘀型) and Disharmony of the thoroughfare and conception vessels pattern(DTCVP, 衝任不調型) each. Conclusions : The results might provide backgrounds and methods for clinical care of acne and follow-up study.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.24 no.3
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• pp.17-26
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• 2011

Objectives : Corydalis tuber has been used for promoting blood circulation and for relieving pain in Oriental medicine. In the present study, we focused on the antimicrobial activity of Corydalis tuber and compared its antimicrobial activity with the processed Corydalis tuber. Methods : Processing of Corydalis tuber was accomplished by immersing in 5% of acetic acid for 12 h and then by roasting at $250^{\circ}C$ for indicated time periods(0-30 min). Minimum inhibitory concentration(MIC) and the zone of growth inhibition were determined against Propionibacterium acnes(P. acnes). Results : The methanolic extracts of Corydalis tuber showed potent antimicrobial effect(MIC 62.5 ${\mu}g/ml$). Its alkaloidal component, dehydrocorydaline, also exhibited antibacterial activity(MIC 25.0 ${\mu}g/ml$). After processing of Corydalis tuber, its inhibitory effect on the growth of P. acnes was significantly enhanced compared with that of unprocessed Corydalis tuber. Furthermore, elevated content of dehydrocorydaline was found in the processed than the unprocessed Corydalis tuber. However, the different roasting minutes effected on antimicrobial activity. The best roasting time of Corydalis tuber was 10 min, while roasting for the time above 15 min resulted in diminishing antimicrobial activity. Thus, it was concluded that the standardized processing condition of Corydalis tuber should be established to obtain enhanced antimicrobial(P. acnes) activity. Conclusion : For antimicrobial effect against P. acnes, the best processing condition of Corydalis tuber is immersing in 5% of acetic acid for 12 h and by roasting at $250^{\circ}C$ for 10 min.

• 한국벤처창업학회:학술대회논문집
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• 2017.11a
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• pp.171-174
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• 2017

오늘날 경제 성장의 발전은 현대인의 삶의 여유를 가져오게 되었으며, 그 중 뷰티산업의 발전은 국가 주력의 산업으로 발전하게 되었다. 이에 뷰티산업의 구성원인 여성의 사회진출의 기회가 증가 되었고, 이로 인해 여성들은 사회 적응을 위한 하나의 방법으로 외모적인 경쟁력을 가져야 했다. 외모에 대한 관심은 물론 매스미디어의 증가, 사회적, 문화적 분위기, 미용기술의 발달 등을 들 수 있다. 외모의 사전적의미로는 겉으로 나타나는 모습으로 외모관리를 위한 영역으로 피부 관리를 꼽을 수 있다. 피부 관리는 미에 관한 현대 여성의 관심의 증가로, 시간과 비용을 투자하여 외적으로 보여주는 관리로서 외모관리의 대표적 부분으로 중요시 되어오고 있다. 피부의 건강관리는 인식도 중요하고 실천도 중요하다. 그러나 인식과 실천의 관계를 구체적으로 연구한 논문은 부족한 실정이다. 특히 성인여성들을 대상으로 건강관리 인식과 실천을 연구하여 피부건강 관리에 대한 올바른 방안을 제시해준 논문이 필요하다고 생각하여 본 연구자는 이 논문을 설계하게 되었다. 본 연구의 조사대상은 서울지역에 거주하는 20세 이상 60세 미만의 신 성인여성을 대상으로 2017년 5월 02일부터 7월 30일까지 2달 동안 설문조사를 실시하였다. 모집단은 서울지역에 사는 성인 여성을 대상으로 총 400부의 설문지를 배포하였다. 그 중 응답이 불량한 사람 49부를 제외되고 351부가 최종 자료로 사용하였다. 연구 결과는 다음과 같다. 첫째, 조사대상자의 인구통계학적 특성을 알아본 결과, 연령은 '21-30세'가 85.2%로 가장 많았고, 결혼 상태는 '미혼'이 89.5%, 직업은 '학생' 67.0%, 최종학력은 '대학교 재학/졸업'이 68.1%, '가계의 월 소득은 '200만원 미만'이 71.8%로 가장 많이 소비되는 것으로 나타났다. 이상은 이혜원 (2009)의 논문과 같이 연령이 낮을수록 피부인식이 높아지며, 이들은 잠정고객으로서 피부 건강에 대한 비용을 높게 지불한다는 결과와 유사하다. 피부 관리실의 특성은 다음과 같다. 피부유형은 '복합성'이 47.6%, 적정 피부 관리 횟수는 '주 2회'가 32.5%, 적정 1회 피부 관리 비용으로는 '5만 원 이하'가 가장 많았으며, 피부 관리의 목적으로는 '여드름관리'가 32.8%, 피부 관리에서 중요하게 인식하는 부분으로는 '생활습관'이 79.8%로 대부분이었고, 피부의 유해요인으로는 '스트레스' 42.7%로 가장 높게 나타났다. 이상은 유유정(2013), 장은영(2013)와 같이 생활습관과 스트레스가 피부 관리에 영향을 준다는 결과와 유사하다.

• Korean Journal of Pharmacognosy
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• v.43 no.4
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• pp.323-327
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• 2012

Acne, also known as Acne vulgaris, is a common disorder of human skin involving the sebaceous gland and Propionibacterium acnes (P. acnes). The purpose of this study was to demonstrate whether anti-acne herbal complex (AAHC), a functional extract from herbal complex can be used for acne treatment as a natural product. We first demonstrated anti-acne activity of AAHC in mouse acne model. Acne was induced by injecting P. acnes on the backside $2{\times}10^7$ CFUs in ICR mice and then the mice were treated with AAHC by dermal application once daily. ACFREE$^{(R)}$ (clindamicin phosphate) was used as a positive control. Treatment with AAHC decreased the P. acnes-induced skin swelling and inflammation. AAHC treatment significantly decreased serum DHT concentration in acne-induced mice. Especially, treatment of 20% AACH in mice was more effected than 40%. We next evaluated the antimicrobial property of AAHC against P. acnes, Staphylcococcus aureus (S.aureus), and Escherichia coli (E. coli). Incubation of P. acnes, S. aureus, and E. coli with AAHC showed minimal inhibitory concentration (MIC) values against the bacterial growth lower. Alamar blue method was also carried for the antibacterial activity. It was effectively MIC level at 6.25% of P. acnes. AAHC effectively inhibited the growth of S. aureus and E. coli at 0.097% on MIC level, respectively. Our results showed the potential of using AAHC as an alternative treatment for antibiotic therapy of acne and the application of AAHC as a herbal medicine for acne treatment.

• Journal of Life Science
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• v.15 no.2 s.69
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• pp.279-286
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• 2005

The Opuntia ficus-indica var. saboten Makino (Cactus) is a tropical or subtropical plant, which is cultivated or grows naturally in Jeju island. It has been widely used as folk medicine for burned wound, edema and indigestion. In addition, its extract has been claimed to have several biological activities including anti-inflammation in oriental medicine. In this study, we examined the antimicrobial activities of the methanol extract of Opuntia ficus-indica var. saboten Makino. The extract showed a broad spectrum of antimicrobial activity against pathogenic bacteria, including antibiotics resistant bacteria (MRSA, R-P. aeruginosa, VRE) and Propionibacterium acnes, yeast, and fungi. The extract retained the activity after heat treatment for 15 min at $100^{\circ}C$ and $121^{\circ}C$ and after extended storage, up to 10 weeks storage period at $4^{\circ}C$ and $25^{\circ}C$, also stably retained its activity. It showed a better inhibitoring effect to the growth of E. coli than sodium benzoate did it at the same concentration. Addition of various salts or metal ions did not affect on its antimicrobial activity. Therefore, the antimicrobial characteristics of the extract can be applicable as a natural preservative and an antimicrobial agent for bacterial disease.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.31 no.4
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• pp.83-95
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• 2018

Objectives : This study was conducted to review about a prevention and management of acne Methods : We searched Korean research database by using keyword 'acne' and 'prevention' or 'management'. Korean research databases were Korean Studies Information Service System(KISS), Research Information Service System(RISS), Korean Medicine Information System(OASIS), National Digital Science Library(NDSL) and Jeonju Municipal Library. We classified studies identified by year, method, contents Results : Among 19 studies, 10 studies were articles and 9 studies were books. The proportion of studies published in 1990s was 26.3%, published in 2000s was 46.3%, published in 2010s was 26.3%. We analyzed 19 studies by classifying 4 categories that consist of Diet Management, Skin Management, Life Management, Exercise Management. In Diet Management, intake of fruits and vegetables was highly recommended, and intake of oily foods and high-fat foods was restricted. In Skin Management, thorough skin cleansing was highly recommended and skin contact was restricted. In Life Management, enough sleep and rest were highly recommended, and smoking was restricted. In Exercise Management, some of exercises to promote blood circulation were recommended. Conclusions : Various methods have been used for prevention and management of acne. It is necessary to systematically study the prevention and management of acne.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.31 no.4
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• pp.65-82
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• 2018

Objectives : The purpose of this study is 1) to explore the general tendency of the herbs or herbal formula for acne treatment in clinical practice 2) and to provide a basis for explaining the principles of acne treatment in Korean Medicine in order to make patients understand more easily based on pharmacological mechanism by analyzing the herbs or herbal formula using for acne treatment in clinical practice. Methods : In the domestic databases (Oriental medicine Advanced Searching Integrated System-OASIS, Korean Traditional Knowledge Portal-KTKP, National Discovery for Science Leader-NDSL, Research Information Sharing Service-RISS), we selected among the papers published using search terms related to "acne". Reports related to sasang constitutional medicine were excluded and a total of 23 papers were finally selected. Results : 29 prescriptions were retrieved from 23 papers. Chungan-tang (淸顔湯) notified formulas ranked first by Cheongsangbangpung- tang (淸上防風湯), Hwangnyeonhaedok-tang (黃連解毒湯), Dangguijakyaksan (當歸芍藥散) and Bipachungpe-eum (枇杷淸肺飮). Commonly used herbs are Forsythiae Fructus (連翹), Scutellariae Radix (黃芩), Gardeniae Fructus (梔子), Ligustici Rhizoma (川芎), and Angelicae Gigantis Radix (當歸). In most patients with inflammatory lesions or excessive flushing, exterior-releasing medicinal (解表藥) and heat-clearing medicinal (淸熱藥) were mainly prescribed for acne treatment, while in patients with acne accompanied hyperkeratinization, Excess sebum secretion, Dysmenorrhea or indigestion, qi-tonifying medicinal (補氣藥) or blood-tonifying medicinal (補血藥) were mainly used. KAGS (Korean Acne Grading System) was the most commonly used method for acne severity assessment. Conclusions : These findings suggest that the herbs or herbal formula for acne treatment in Korean Medicine is prescribed differently according to the cause and symptoms of acne in a unique way for control the whole body balance. Also, our findings could provide a basis for explaining the principles of acne treatment in Korean Medicine in order to make patients understand more easily based on pharmacological mechanism in clinical practice.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.29 no.1
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• pp.93-102
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• 2016

Objective : This study performed to evaluate that duration of acne scars effects on Qualitative Global Acne Scarring Grading System(QGASC) grade changing and treatment duration. Also this study performed to evaluate the relationship between dysmenorrhea and acne scars, and the effects of each treatment on treatment duration and Korean Acne Grading System(KAGS) and QGASC grade.Methods : 95 patients with acne scars were understood by medical records and case photos, and we evaluated pre and post acne scar grade based on KAGS and QGASC. We analyzed data by SPSS 22 for windows program, and figured out the relation between duration of acne and QGASC grade and treatment duration by Spearman correlation analysis, and performed Mann-Whitney U-test to figure out the relation between dysmenorrhea and QGASC grade. We performed Wilcoxon test to evaluate that treatment of acne scars effects on KAGS grade and QGASC grade, and performed Kruskal-Wallis test to figure out the difference of each treatment which effects on KAGS grade and QGASC grade, and performed Post-hoc analysis to figure out the group comparison.Results & Conclusions : QGASC grade depending on duration of acne was statistically significance. Subcision and Microneedle Therapy System(MTS) and pharmacopuncture were significantly improved acne scars. Other treatments were significantly more effective than subcision and pharmacopuncture combined treatment. Treatment duration of Subcision and MTS combined treatment was significantly most shortest than other treatment.

• Journal of Life Science
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• v.17 no.7 s.87
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• pp.915-922
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• 2007

In order to discover physiologically active substance, we investigated a powder obtained by processing of Opuntia ficus-indica var. saboten Makino trunk. The powder was treated by sulfuric acid and then extracted by several solvents such as methanol, methylene chloride, ethanol etc. Among them, the best antimicrobial activity was showed by methylene chloride extract. To identify materials exhibiting physiological activation, the acid hydrolyzed extract was separated by 7 fractions through preparative silica gel TLC. The effective fraction exhibiting the best broad antimicrobial activity was identified, named as MBT-01108. From structural analysis of the products extracted to acid hydrolysis, a compound exhibiting the antimicrobial activities is identified to levulinic acid. Levulinic acid isolated from Opuntia ficus-indica var. saboten Makino may be applicable as a natural preservative of food or cosmetic and for prevention of bacterial diseases, an ingredient of acne, ageing and whitening cosmetics and an antimicrobial agent.