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http://dx.doi.org/10.5352/JLS.2007.17.7.915

Identification of Anti-Microbial Material Originated from Opuntia ficus-indica var. saboten Makino  

Kim, Hae-Nam (Department of Beauty Care, Masan College)
Cho, Dae-Won (Division of Chemistry, Physics, Pusan National University)
Yoon, Ung-Chan (Division of Chemistry, Physics, Pusan National University)
Jun, Hong-Ki (Division of Biological Science, Pusan National University)
Publication Information
Journal of Life Science / v.17, no.7, 2007 , pp. 915-922 More about this Journal
Abstract
In order to discover physiologically active substance, we investigated a powder obtained by processing of Opuntia ficus-indica var. saboten Makino trunk. The powder was treated by sulfuric acid and then extracted by several solvents such as methanol, methylene chloride, ethanol etc. Among them, the best antimicrobial activity was showed by methylene chloride extract. To identify materials exhibiting physiological activation, the acid hydrolyzed extract was separated by 7 fractions through preparative silica gel TLC. The effective fraction exhibiting the best broad antimicrobial activity was identified, named as MBT-01108. From structural analysis of the products extracted to acid hydrolysis, a compound exhibiting the antimicrobial activities is identified to levulinic acid. Levulinic acid isolated from Opuntia ficus-indica var. saboten Makino may be applicable as a natural preservative of food or cosmetic and for prevention of bacterial diseases, an ingredient of acne, ageing and whitening cosmetics and an antimicrobial agent.
Keywords
Cactus (Opuntia ficus-indica var. saboten Makino); Anti-microbial activity; Levulinic acid; (4-oxopentanoic acid); MBT-01108; Physiologically active material;
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