• Food Science and Preservation
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• v.9 no.4
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• pp.429-433
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• 2002

The D-xylulokinase from Candida sp. L-16 was purified through a sequence of ammonium sulfate fractionation, DEAE-cellulose chromatography, Sephadex G-100 and Sephadex G-200 gel filtration. The specific activity of the purified Dxylulokinase was increased to 23.2 fold and the yield was 11.2%. The enzyme was showed to be a single protein band by SDS-PAGE. The molecular weight of the enzyme was 150,000 dalton, this enzyme was identified to be a dimer with two subunits. The optimum conditions of the enzyme were pH 8.0 and 40$\^{C}$, respectively. The enzyme was relatively stable between pH 7.0 to pH 9.0, but it was unstable over 30$\^{C}$. The enzyme showed substrate specificity on D-xylulose, D-arabinose and D-ribose, Km value and Vmax for D-xylulose were 0.042 mM and 117 units/ml, respectively. The activation energy of the enzyme was 4.75 Kcal/mol. The one was inhibited by metabolic intermediates such as 6-phosphogluconic acid, 2-keto-gluconic acid. The enzyme was activated by EDTA and thiol compounds such as cysteine-HCI, DTT and glutathione.

• Particle and aerosol research
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• v.9 no.2
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• pp.39-50
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• 2013

Real-time aerosol measuring instruments have been widely used for the measurement of atmospheric aerosol, diesel particulate matter, or material synthesis. A scanning mobility particle sizer (SMPS) measures the number size distribution of particles using electrical mobility detection technique. An aerodynamic particle sizer (APS) is used to determine the number concentration and the mean aerodynamic diameter of test particles. An electrical low-pressure impactor (ELPI) is a multi-stage impaction device to separate airborne particles into aerodynamic size classes using particle charging and electrical detection techniques. In this study, the performance of these instruments were evaluated to assess their ability to obtain mass concentrations from particle number concentration measurements made as a function of particle size. The effect of determination of particle density on the measurement of mass concentration was investigated for the three instruments.

• Korean Journal of Food Science and Technology
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• v.19 no.5
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• pp.430-434
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• 1987

Fatty acid fraction rich in ${\omega}-3$ polyunsaturated fatty acids (${\omega}-3$, PUFA), especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) could be obtained by saponification of fish oil in ethanol containing alkali hydroxide followed by cooling and filtration of the resultant solution. Fatty acid compositions of fish oil and the concentrates suggest that the ratio of number of double bonds to carbon number in a fatty acid molecule is a more important factor than the degree of unsaturation or the chain length in determining the solubility of fatty acid salts in ethanol. Water content in ethano1 affected significantly the efficiency of the separation with respect to yield and content of EPA and DHA in the concentrates; the lower the water content, the higher the efficiency. It was, however, influenced little by cooling procedure and temperature which the saponified solution experienced during the crystallization. Under an optimal condition, the contents of EPA and DHA in the concentrate increased by 2.4 and 2.5 times, respectively, as compared with those in sardine oil.

• KSBB Journal
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• v.25 no.5
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• pp.429-436
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• 2010

Keratinase is widely used in certain industrial applications. The present study sought to improve the culture conditions of Bacillus subtilis SMMJ-2 to facilitate mass production of keratinase. Strain SMMJ-2 was irradiated by ultraviolet light and the resulting isolates were tested for keratinase activity. Isolates displaying elevated keratinase activity were selected and used to determine the optimum temperature (24, 30, 37, 45, $55^{\circ}C$) for bacterial keratinase production during a 4 day incubation period. The highest enzyme activity (55 units/mL/min), from a Bacillus subtilis SMMJ-2 mutant (mutant No. 2) was demonstrated following incubation at $30^{\circ}C$. The effects of carbon and nitrogen sources on keratinase production were confirmed by measuring the enzyme activity from the culture broth of the mutant strain cultured in various media containing different carbon source and nitrogen sources during a 4 day period. The optimal medium composition for producing keratinase consisted of 1% glucose, 0.7% $K_2HPO_4$, 0.2% $K_2HPO_4$, and 1.2% soybean meal. Optimal initial pH and temperature for producing keratinase were 7.0 and $30^{\circ}C$, respectively. Keratinases produced by B. subtilis SMMJ-2 and the mutant No. 2 were purified from the culture broth which used soybean meal as a nitrogen source. Membrane ultrafiltration, DEAE-sephacel ion exchange and Sephadex G-100 gel chromatography were used to purify the enzymes. The purified keratinases from both B. subtilis SMMJ-2 and the mutant No. 2 showed single bands and their molecular weights were estimated as 28 kDa and 42 kDa, respectively on SDS-polyacrylamide gel electrophoresis.

• Journal of KIISE:Databases
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• v.31 no.6
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• pp.567-584
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• 2004

As XML becomes popular as the way of presenting information on the web, how to secure XML data becomes an important issue. So far study on XML security has focused on security of data communications by using digital sign or encryption technology. But, it now requires not just to communicate secure XML data on communication but also to manage query process to access XML data since XML data becomes more complicated and bigger. We can manage XML data queries by access control technique. Right now current XML data access control only deals with read operation. This approach has no option to process update XML queries. In this paper, we present XML access control model and technique that can support both read and update operations. In this paper, we will propose the operation for XML document update. Also, We will define action type as a new concept to manage authorization information and process update queries. It results in both minimizing access control steps and reducing memory cost. In addition, we can filter queries that have no access rights at the XML data, which it can reduce unnecessary tasks for processing unauthorized query. As a result of the performance evaluation, we show our access control model is proved to be better than other access control model in update query. But it has a little overhead to decide action type in select query.

• Journal of KIISE:Databases
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• v.36 no.2
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• pp.112-122
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• 2009

The EPCglobal leading the development in RFID standards proposed Event Cycle Specification (ECSpec) and Event Cycle Reports (ECReports) for the standard about RFID middleware interface. ECSpec is a specification for filtering and collecting RFID tag data and is treated as a Continuous Query (CQ) processed during fixed time intervals repeatedly. ECReport is a specification for describing the results after ECSpec is processed. Thus, it is efficient to apply Query Indexing technique designed for the continuous query processing. This query index processes ECSpecs as data and tag events as queries for efficiency. In logistics environment, the similar or same products are transferred together. Also, when RFID tags attached to the products are acquired, the acquisition events occur massively for the short period. For these properties, it is inefficient to process the massive events one by one. In this paper, we propose a technique reducing similar search process by considering tag events which are collected by the report period in ECSpec, as a range query. For this group processing, we suggest a queuing method for collecting tag events efficiently and a structure for generating range queries in the queues. The experiments show that performance is enhanced by the proposed methods.

• Korean Journal of Ecology and Environment
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• v.47 no.spc
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• pp.1-9
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• 2014

Lake Suwa is a natural lake which is well-known for sightseeing and fisheries. It had suffered severe eutrophication during 1960s and 1970s with the occurrence of cyanobacterial blooms and the extinction of some benthic animals. Since 1980 water quality has been improved due to efforts of local government, scientists, and citizens. Of various methods that were attempted to improve the water quality of Lake Suwa biological methods received much attention, because it can improve the lake ecosystem integrity and fisheries in addition to the water quality. The aim of this paper is to introduce the biological methods for water quality improvement that had been employed in Lake Suwa, Japan, and their contribution to the economic benefit of local residents. Until now a significant restoration of water quality has been achieved, but there are insufficient recovery of the sediment and biota due to anoxic hypolimnion of the lake. This study proposed suspended cage culture of bivalves as a feasible method of water quality improvement. Increased grazing by bivalves will contribute to the improvement of water quality and fisheries production, which will contribute both to the ecological restoration and economy of local residents.

• Korean Journal of Food Science and Technology
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• v.34 no.4
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• pp.552-558
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• 2002

Polyphenol oxidase from Flammulina velutipes was purified and characterized. Purification of polyphenol oxidase was achieved by ammonium sulfate precipitation, Superdex G-200 gel filtration chromatography, Phenyl superose affinity chromatography, Mono-Q anion exchange chromatography and Superdex S-200 gel filtration chromatography on FPLC. After these purification steps specific activity of purified polyphenol oxidase increased to 199.1 units/mg. Polyphenol oxidase from F. velutipes was composed of a single polypeptide with molecular weight of about 40 kDa. Optimum pH and temperature for the enzyme reaction were found to be 6.0 and $25^{\circ}C$, respectively. The activity of the enzyme gradually decreased at acidic pH between 3 and 5, and the enzyme lost its activity at alkaline pH between 8 and 10. This enzyme exhibited high substrate specificity to o-diphenols. Km-values for L-DOPA and caffeic acid were found to be 3.97 mM and 1.78 mM, respectively. 2-mercaptoethanol, L-ascorbic acid, sodium bisulfite, EDTA and $Mg^{2+}$ inhibited the activity of pholyphenol oxidase and $Cu^{2+}$, $Fe^{2+}$, $Zn^{2+}$ and $Ni^{2+}$ increased enzyme activity. The activity of enzyme was well maintained at $-70^{\circ}C$ for over 4 months, and at $-20^{\circ}C$ for 1 months.

• Journal of Biomedical Engineering Research
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• v.21 no.5
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• pp.441-448
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• 2000

방출단층영상 재구성을 위한 최대우도 기대값최대화(maximum likelihood expectation maximization, MLEM) 방법은 영상 획득과정을 통계학적으로 모델링하여 영상을 재구성한다. MLEM은 일반적으로 사용하여 여과후역투사(filtered backprojection)방법에 비해 많은 장점을 가지고 있으나 반복횟수 증가에 따른 발산과 재구성 시간이 오래 걸리는 단점을 가지고 있다. 이 논문에서는 이러한 단점을 보완하기 위해 계산시간을 현저히 단축시킨 배열된부분집합 기대값최대화(ordered subsets expectation maximization. OSEM)에 Gibbs 선행치인 membrance (MM) 또는 thin plate(TP)을 첨가한 OSEM-MAP (maximum a posteriori)을 구현함으로써 알고리즘의 안정성 및 재구성된 영상의 질을 향상시키고자 g나다. 실험에서 알고리즘의 수렴시간을 가속화하기 위해 투사 데이터를 16개의 부분집합으로 분할하여 반복연산을 수행하였으며, 알고리즘의 성능을 비교하기 위해 소프트웨어 모형(원숭이 뇌 자가방사선, 수학적심장흉부)을 사용한 영상재구성 결과를 제곱오차로 비교하였다. 또한 알고리즘의 사용 가능성을 평가하기 위해 물리모형을 사용하여 PET 기기로부터 획득한 실제 투사 데이터를 사용하였다.

• Korean Journal of Breeding Science
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• v.41 no.3
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• pp.252-260
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• 2009

Rice is the most important cereal crop not only in supplying the basic staple food for more than half of the world's population but also as a model plant for functional genomic studies of monocotyledons. Although rice transformation method using A. tumefaciens has already been widely used to generate transgenic plants, the transformation rate is still low in most Korean elite cultivars. We made several modifications of the standard protocol especially in the co-cultivation step to improve the efficiency of the rice transformation. The co-culture medium was modified by the addition of three antioxidant compounds (10.5 mg/L L-cysteine, 1 mM sodium thiosulfate, 1 mM dithiothreitol) and of Agrobacterium growth-inhibiting agent (5 mg/L silver nitrate). Co-cultivation temperature ($23.5^{\circ}C$ for 1 day, $26.5^{\circ}C$ for 6 days) and duration (7 days) were also changed. The plasmid of pMJC-GB-GUS carrying the GUS reporter gene and the bar gene as the selectable marker was used to evaluate the efficiency of the transformation. After co-cultivation, a high level of GUS gene expression was observed in calli treated with the modified method. It is likely that those newly added compounds helped to minimize the damage due to oxidative bursts during plant cell-Agrobacterium interaction and to prevent necrosis of rice cells. And the transformation rate under the modified method was also remarkably increased approximately 8-fold in Heungnambyeo and 2-fold in Ilmibyeo as compared to the corresponding standard method. Furthermore, we could produce the transgenic plants stably from Ilpumbyeo which is a high-quality rice but its transformation rate is extremely low. Transformation and the copy number of transgenes were confirmed by PCR, bar strip and Southern blot analysis. The improved method would attribute reducing the effort and the time required to produce a large number of transgenic rice plants.