• Proceedings of the Korean Environmental Sciences Society Conference
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• 2008.11a
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• pp.198-201
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• 2008

매생이의 에탄올 추출물의 기능적 특성과 추출액의 플라보노이드와 페놀함량과의 상관성을 검토하고, 아질산염 분해능과 효소의 영향 등을 측정하였다. 매생이 에탄올 추출액의 클로로필 함량은 물추출보다 에탄올 추출의 경우가 높았으나, 플라보노이드 함량은 물추출액에서 약간 높게 나타났다. 추출액의 TPC함량은 TFC함량이 높을수록 높게 나타났으나 직접 비례하지는 않았다. 그리고 매생이에 함유된 아질산염 함량 측정결과와 아질산염 분해능 결파 비교로부터 에탄올 추출의 경우에는 아질산염 분해능이 시료자체의 아질산염 함량과 직접적인 영향이 없는 것으로 보였으며, 매생이 에탄올 추출액이 나타내는 아질산염 분해작용은 낮은 pH에서 높게 나타났으나, 매생이 자체에 존재하는 효소의 관여는 극히 미약하였다.

• The Korean Journal of Food And Nutrition
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• v.10 no.3
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• pp.382-386
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• 1997

In the present study, an attempt was made to investigate the effects of steeping treatment on browning of onion hydrolysate. After steeping treatment with solvents, phenols content of methanol and ethanol were showed 25.1mg/ml and 24.9mg/ml. And absorbance of methanol and ethanol was showed 0.26 and 0.22. L and b value of treatment with methanol and ethanol were lower than other solvents. Browning reaction of onion hydrolysate was decreased with increasing concentration of ethanol. Treatment of above 80% ethanol was effective to remove phenols and was showed a low color intensity. Treatment with 80% ethanol was more effective than addition of cystein to remove phenols and to decrease browing degree.

• Korean Chemical Engineering Research
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• v.50 no.1
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• pp.141-148
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• 2012

In order to study the utilization of brown seaweed Laminaria japonica as an alternative renewable feedstock for bioethanol production, the properties of acid hydrolysis and ethanol fermentation were investigated. The acid hydrolysis enhanced the final yield of fermentable sugars, which led great increase of ethanol productivity. The maximum yield of reducing sugars reached 135 mg/g-dry Laminaria japonica after 1.0N sulfuric acid-hydrolysis at $130^{\circ}C$ for 6 h. The Saccharomyces cerevisiae (ATCC 24858) could ferment $C_6$-sugars like glucose, galactose and mannose into ethanol, but not $C_5$-sugars like arabinose and xylose. Optimal fermentation time varied with sugars; 48 h for glucose, 72 h for galactose, and 96 h for mannose. Nevertheless, the ethanol yield from the hydrolysate reached 242 mg/g-dry Laminaria japonica after fermentation by the S. cerevisiae at $35^{\circ}C$ for 96 h, which corresponds to approximately 4 times more than the theoretical yield from total reducing sugars in the hydrolysates. It indicates that the non-reducing sugars or oligosaccharides dissolved in the hydrolysate played an important role in producing bioethanol. The ethanol concentration linearly increased from 2.4 to 9.2 g/L, while the ethanol yield per dry weight of biomass decreased from 242 to 185 mg/g, with increasing the ratio of biomass to acid solution from 1 to 5% (w/v). The bioethanol yield estimated was approximately 7,400~9,600 kg/ha/year, and indicated that Laminaria japonica is a promissing feedstock for bioethanol production.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.154-154
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• 2003

국내산 홍화씨를 부위별(Whole, Coat and Endosperm)로 분리한 후 추출용매, 볶음조건, 효소가수분해 등의 처리조건을 달리하여 추출된 추출물의 이화학적특성과 기능성성분 함량을 분석, 비교하였다. 홍화씨 부위별추출물의 고형분함량은 60%에탄올배유부분추출물이 11.29%로, 19$0^{\circ}C$, 30분 볶음처리배유추출물이 14.53%로, amyloglucosidase 처리배유추출물이 24.21%로 높은 고형분이 추출되었다. 총페놀함량은 추출용매의 에탄올농도가 증가함에 따라 증가하는 경향이었고, 80% 에탄올점질추출물이 965mg%로 가장 높았으며, 21$0^{\circ}C$, 30분 볶음처리껍질추출물이 756 mg%로, celluase처리껍질추출물이 1170mg%로 높은 함량이었다. 총플라보노이드함량은 80%에탄올전체추출물이 317 mg%로, 21$0^{\circ}C$, 30분 볶음 처리배유추출물이 488 mg%로, $\beta$-amylase 처리전체추출물이 554 mg%로 높은 함량이었다. 유리당 중 sucrose함량은 21$0^{\circ}C$, 10분 볶음처리배유추출물이 123.4 mg%로, 60%에탄올배유추출물이 57.0 mg%로, Celluase 처리배유추출물이 67.1 mg%로 가장 높은 함량이었고, 또한 glucose, fructose, xylose 및 arabinose 등도 함유하고 있었다. 유기산 중 citric acid 함량은 20%n 에탄올배유추출물이 243.3 67.1 mg%로, 21$0^{\circ}C$, 30분 볶음처리전체추출물이 76.3 mg%로, amyloglucosidase 처리배유추출물이 699.3 mg%로 가장 높은 함량이었고, 또한, oxalic, malic, succinic, acetic 및 fumaric acid 등도 확인되었다. Serotonin 화합물 중 serotonin- I 함량은 100% 에탄올껍질추출물이 431 mg%로, 21$0^{\circ}C$, 10분 볶음처리껍질추출물이 192 mg%로, amyloglucosidase 처리껍질추출물이 256 mg%로 가장 많았다. 또한, Serotonin-II함량은 100%에탄올껍질추출물이 763 mg%로, 17$0^{\circ}C$, 10분 볶음처리전체추출물이 312 mg%로, amyloglucosidase 처리껍질추출물이 456 mg%로 가장 많았다. Acacetin 함량은 80%에탄올배유추출물이 34.9 mg%로, 21$0^{\circ}C$, 30분 볶음처리배유추출물이 221.0 mg%로, amyloglucosidase 처리배유추출물이 27.8 mg%로 가장 많았다.

• KSBB Journal
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• v.24 no.5
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• pp.483-488
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• 2009

The Brown-algae polysaccharide consisting of alginate and laminaran is usable as high bio-ethanol production if hydrolyzed to monomer unit. The objective of this study is to produce bio-ethanol from brown-algae using enzymatic saccharification. Bio-ethanol was produced by Saccharomyces cerevisiae KCCM 1129 and Pachysolen tannophilus KCTC 7937 strains. The substrate used Laminaria japonica, Sargassum fulvellum and Hizikia fusiformis. We isolated a new alginate lyase and laminaran lyase producing microorganism for hydrolysis of brown-algae from southern sea of Gijang. The reducing sugar was obtained 1.90 g/L from Laminarin japonica 20 g/L that used enzyme from Bacterium antarctica. In pretreatment of the most suitable brown-algae for ethanol production, ethanol concentration of 0.93 g/L and yield of 4.65% were obtained in condition of Laminaria japonica in medium.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.84-84
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• 2018

인공재배 매실나무겨우살이의 의약 화장품의 소재 및 산업화 가능성을 검토하기 위해 80% 에탄올 및 증류수 초음파추출물의 Tyrosinase 저해활성을 측정하였다. 인공재배한 매실나무겨우살이(PM, 동결건조) 및 자연산 굴참나무겨우살이(QM, 열풍건조, $30^{\circ}C$, 7day)의 80% 에탄올, 증류수 초음파추출물에 대한 tyrosinase 저해활성을 측정한 결과, 물 추출물의 경우 100 ppm의 농도에서 PM과 QM 모두 5분이 경과 하였을 때 PM은 88.37%, QM은 87.69%로 최대치를 나타내었고, 농도와 시간이 증가함에 따라 tyrosinase 저해활성이 감소하는 경향을 보여주었다. PM 80% 에탄올 추출물은 5분이 경과하였을 때 2,500ppm이 92.08%로 최대값을 보여주었고 이후 시간이 지남에 따라 저해활성이 감소되는 경향을 나타내었다. 반면 5,000ppm은 5분이 경과하였을 때 66.38%로 최소값을 나타낸 반면, 20분까지 저해활성이 증가하였고 10분이 경과한 이후로 거의 일정하게 유지되어 지속적으로 감소되는 1,000ppm보다 높은 저해효과를 보여 주었다. QM 80% 에탄올 추출물은 5분이 경과하였을 때 1,000ppm이 88.14%로 가장 높았고 5,000ppm은 63.49%로 가장 낮은 저해활성을 보여주었다. 15분까지 1,000ppm이 가장 높은 저해활성을 보여주었으나 꾸준히 감소한 반면, 2,500ppm의 경우 시간이 경과함에 따라 비교적 일정한 저해활성을 보여주어 15분이 경과한 이후로 2,500ppm의 저해활성이 가장 높게 나타났다. 25분이 경과하였을 때 2,500ppm은 70.27%로 가장 높은 저해활성을 나타내었고, 5,000ppm은 30.09%로 가장 낮은 저해활성을 보여주었다. 본 연구 결과에 따르면, 물 추출물의 경우 겨우살이의 농도가 낮을수록 더 높은 tyrosinase 저해활성을 나타내었고, 80% 에탄올 추출물의 경우 2,500ppm에서 가장 높은 저해활성을 나타내어 의약 화장품의 소재 및 산업화 가능성이 높음을 확인할 수 있었다.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.10
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• pp.1869-1879
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• 2000

A series of experiments were conducted for modeling the fate and effect of the coupled oxidation reduction reaction of ethanol and propionate recognized as important intermediates in anaerobic degradation metabolism. Anaerobic kinetics for conversion of propionate and the interaction with ethanol were investigated using the model of specific substrate priority utilization effect. Seed cultures for the experiment were obtained from an anaerobically enriched steady-state propionate master culture reactor (HPr-MCR), ethanol-propionate master culture reactor (EtPr-MCR) and glucose master culture reactor (Glu-MCR). Experiments were consisted of four phases. Phase I, II and III were conducted by fixing the propionate organic loading as 1.0 g COD/L with increasing ethanol loading of 0, 100, 200, 400 and 1,000 mg/L, to find metabolic interaction of ethanol and propionate degradation by each enriched anaerobic culture. In phase IV, different mixing ratios of Glu-MCR and HPr-MCR cultures with fixed propionate organic loading, 1.0 g COD/L, were applied to observe the propionate degradation metabolic behavior. In the results of this study, different pathways of propionate and ethanol conversion were found using a modified competitive inhibition kinetic model. Increase of $K_{s2}$ value reflected the formation of acetate followed by ethanol degradation. In addition. $K_3$ value was increased slightly as the reactions of acetate formation and degradation were occurred in acetoclastic methanogenesis.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2012.05a
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• pp.100-103
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• 2012

The blending method that is an addition of small quantity of fuel was used to increase the performance of green propellant thruster. 90 wt.% hydrogen peroxide as a green propellant was selected, and ethanol was used as a blended fuel. The o/f ratio was chosen as 50 which has higher theoretical performance than 98 wt.% hydrogen peroxide. The chamber temperature of blended hydrogen peroxide was higher than adiabatic chamber temperature of hydrogen peroxide. Therefore, performance can be improved by ethanol blending. Several catalyst and its support were compared to find appropriate catalyst for decomposition and combustion of ethanol blended hydrogen peroxide. As a experimental results, Pt was suitable, but $MnO_2$ had a chamber instability when it was reused. The ${\alpha}-Al_2O_3$ which is high heat-resistant support showed very unstable performance in both Pt and $MnO_2$ catalyst since it has low decomposition performance.

• Journal of the Korean Wood Science and Technology
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• v.38 no.6
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• pp.561-567
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• 2010

In this study, we evaluated optimal conditions for ethanol production of the spruce hydrolysate (SH) obtained from diethyl oxalate pretreatment. Fermentable sugar concentration in SH was 29.04 g/${\ell}$ except arabinose. Monosaccharides obtained from the oligomer degradation were mainly mannose (39.26 g/${\ell}$) and galactose (12.83 g/${\ell}$). Concentration of 5-HMF and furfural which are inhibitors on ethanol fermentation were 0.09 g/${\ell}$ and 0.04 g/${\ell}$ respectively. Concentration of acetic acid and total phenolic compounds in SH were 1.4 g/${\ell}$ and 2.83 g/${\ell}$. Ethanol production using hydrolysate was 11.7 g/${\ell}$ at optimal pH 6.0 after 48 h. Specific ethanol production was 0.15 (g/(${\ell}^*h$)) at pH 5.0 and 5.5. while that was 0.24 (g/(${\ell}^*h$)) at pH 6.0. Specific ethanol production has difference depend on initial pH for fermentation. Ethanol production was 14.3 g/${\ell}$ after 48 h when xylanase 20 IU was added in SH for degradation of oligomer during fermentation. It implied that ethanol production increased by 22.2% compare with control (without xylanase).

• Journal of Applied Biological Chemistry
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• v.56 no.4
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• pp.245-247
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• 2013

The potential of the red ginseng marc (RGM) for the production of bio-ethanol using enzymatic hydrolysis and fermentation without any additional nutrients was investigated. Reducing sugar content in RGM treated with Viscozyme and Flavourzyme was 101.1 g/L and was much higher than that (7.2 g/L) in intact RGM. When enzymatically hydrolyzed red ginseng marc (ERGM) was fermented with commercially available dry yeast at $25^{\circ}C$ for 7 days, the final ethanol concentration reached 29.3 g/L with ethanol yield at 0.274 g of ethanol per 1 g of solubilized total sugar. Ethanol concentration and ethanol yield of ERGM were drastically increased over 1000% and 50%, respectively than those of RGM.