• Title/Summary/Keyword: 억제재배

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Selection of insecticides for controlling Lycoriella mali in Letinula edodes sawdust cultivation (표고 톱밥재배에서 긴수염버섯파리 (Lycoriella mali)의 방제약제 선발)

  • Kim, Gil-Hah;Yoo, Jeong-Su;Koo, Chang-Duck;Lee, Sang-Gil;Park, Ji-Doo
    • The Korean Journal of Pesticide Science
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    • v.5 no.2
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    • pp.62-66
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    • 2001
  • The thirteen commercial insecticides to Lycoriella mali were investigated on their insecticidal activities and mycelial growth of two Letinula edodes strains. For the adults, insecticides showing over 95% insecticidal activity were chlorpyrifos-methyl, fenthion, fenitrothion, benfuracarb, furathiocarb and deltamethrin. For tile larvae, diflubenzuron and cyromazine showed over 90% insecticidal activity. Fenthion, benfuracarb, furathiocarb, deltamethrin, diflubenzuron and cyromazine did not affect the mycelial growth of L. edodes strain, in Imhyup 1 variety. And deltamethrin, diflubenzuron and cyromazine did not affect that in Sanlim-5. Insecticides showing over 80% residual effect for 14 days were benfuracarb to the adults and diflubenzuron and cyromazine to the larvae. Control effect of furathiocarb, fenthion, benfuracarb, deltamethrin, diflubenzuron and cyromazine against larvae of Lycoriella mali showed 90% ten days after application under sawdust cultivation. Among them, benfuracarb, fenthion and furathiocarb revealed excellent control effect against adults. These results indicate that benfuracarb, fenthion, and furathiocarb can be used for the control of Lycoriella mali in the field. However, further studies are needed on the effect of insecticides treatment oil fruit-body yield and chemical residue in the mushroom tissues.

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Effect of Soil Moisture and Texture on Saikosaponins Content and Antioxidative Enzyme Activities in Bupleurum falcatum L. (재배토양의 수분 및 토성이 시호의 생육상황 및 항산화효소 활성에 미치는 영향)

  • 정형진;신동현;이인중;권순태;임종국;유정민;정규영;김길웅
    • Korean Journal of Plant Resources
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    • v.13 no.2
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    • pp.95-103
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    • 2000
  • To study the effects of soil moisture and texture on characteristics of growth, content of saikosaponins and activity of antioxidative enzymes in Bupleurum falcatum L., content of saikosaponins(a, c and d) and activities of superoxide dismutase(SOD) and peroxidase(POD) were investigated with two Bupleurum genotypes(Jangsoo and Samdo). Two Bupleurum genotypes were grown under different soil moisture(deficit, normal, surplus) and soil texture(sandy, sandy loam, loam) conditions. Among the tested soil conditions, dry weight accumulation rate of both cultivars could be ranked in the order surplus > normal > deficit soil for soil moisture and sandy > sandy loam > loam for soil texture. Under the surplus soil condition, growth retardation of Samdo cultivar was more severer than that of Jangsoo. Furthermore, content of saikosaponin a, d, and c also could be ranked in the order deficit > normal > surplus and sandy > sandy loam > loam for soil moisture and texture, respectively. Although both Jangsoo and Samdo cultivars grown under water deficit condition showed the highest POD and SOD activity, in general POD and SOD activity in both shoot and root was remarkably high in Jangsoo cultivar compared with Samdo. Saikosaponin content of root was positively correlated with POD and SOD. However, shoot and root length were negatively correlated with POD.

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Effects of Heavy Metal Concentrations on Seed Germination and Rhizome Development of Campanula takesimana Nakai (중금속 농도가 섬초롱꽃 종자 발아 및 유근 발달에 미치는 영향)

  • Kim, Tae Hyun;Kim, Do Hyun;Im, Hyeon Jeong;Choi, Kyu Seung;Kim, Sang Geun;Song, Chi Hyeon;Oh, Beom Seok;Kim, Yang Su;Song, Ki Seon;Won, Chang O
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.39-39
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    • 2019
  • 섬초롱꽃은 울릉군에 한정적으로 분포하는 여러해살이풀이다. 산림청에서는 약관심종(LC) 등급의 희귀식물이자 특산식물로 분류하고 있으며, 꽃의 관상가치가 특히 높아 화단용 소재, 절화용, 화분재배 등으로 흔히 이용되고 있다. 따라서 본 연구는 섬초롱꽃의 대량증식 시, 중금속이 미칠 수 있는 영향에 대해 파악하고자 수행하였다. 실험에 사용한 중금속은 카드뮴(Cd), 철(Fe) 두 종류이며, 두 종류 모두 1% 한천(agar)배지에 10, 25, 50, 100, 200 mg/L 농도로 녹여 사용하였다. 발아율 및 유근 생장을 위해 종자 및 유묘를 치상 후, $25^{\circ}C$ 항온 생장상에서 발아율 및 유근 발달을 관찰하였다. 발아율 측정결과, 아무처리도하지 않은 1% 한천(agar) 배지에서 $62.7{\pm}7.4%$로 가장 높았고 중금속 농도가 높아질수록 감소하였다. 카드뮴(Cd) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 $17.3{\pm}1.3$, $2.7{\pm}1.3$, $1.3{\pm}1.3$, 0, 0%로 나타났다. 한편, 철(Fe) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 $46.7{\pm}4.8$, $24.0{\pm}2.3$, $36.0{\pm}6.9$, $20.0{\pm}2.3$, 0%로 나타났다. 유근의 평균 생장 길이(mm)는 아무처리도 하지 않은 1% 한천(agar) 배지에서 19.6 mm로 가장 높았고, 중금속 농도가 높아질수록 낮게 나타났다. 카드뮴(Cd) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 4.6 mm, 1.9 mm, 0.7 mm, 0.9 mm, 0 mm 생장한 것으로 나타났다. 한편, 철(Fe) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 15.9 mm, 8.6 mm, 8.1 mm, 0.9 mm, 0 mm 생장한 것으로 나타났다. 실험결과를 종합해 볼 때, 섬초롱꽃 종자 발아 및 유근 생장의 억제는 카드뮴(Cd) 처리구에서 높게 나타났고, 철(Fe) 처리구에서는 비교적 영향을 덜 미치는 것으로 나타났다. 특히 카드뮴은 10 mg/L 농도만 처리하더라도 약 3.6~4.2배의 발아율 생장율 감소가 나타나, 섬초롱꽃 대량증식 시, 치명적일 것으로 판단된다. 본 실험의 결과는 섬초롱꽃 종자를 이용한 대량증식 및 유묘재배에 있어, 토양을 선택하는데 중요한 기초자료로서 활용될 것으로 기대된다.

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Recovery of Streptococcus Mutans Biofilm after Photodynamic Therapy with Erythrosine and LED Light Source (Erythrosine과 LED를 이용한 광역동 치료 후 Streptococcus mutans 바이오필름의 회복)

  • Yongwook, Shin;Howon, Park;Juhyun, Lee;Siyoung, Lee
    • Journal of the korean academy of Pediatric Dentistry
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    • v.49 no.2
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    • pp.149-157
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    • 2022
  • The aim of this study was to evaluate the effects of erythrosine-mediated photodynamic therapy (PDT) on Streptococcus mutans biofilm recovery by counting its colony-forming units (CFUs) and via confocal laser scanning microscopy analysis at different time points following PDT. In PDT, photosensitizer was an erythrosine. S. mutans ATCC25175 biofilms were irradiated using an LED curing light. Chlorhexidine (CHX) was used as positive control. After each antimicrobial treatment, samples were cultured to allow biofilm recovery. Viability was measured by calculating the CFU counts after treatment and after every 3 hours for up to 24 hours. Immediately after treatment, the PDT and CHX groups showed equally significant decreases in S. mutans CFU counts compared to the negative control. After 12 hours of reculture, the PDT group showed no significant difference in the decrease in CFU count compared to the negative control, whereas the CHX group showed significantly lower CFU counts throughout the 24-hour period. Erythrosine-mediated PDT can effectively inhibit S. mutans biofilm formation. However, biofilm recovery occurred earlier in the CHX group after PDT. This study provides insights into the clinical effectiveness of PDT in preventing dental caries.

Researching the Occurrence Potential of Autoite for Living Modified OrganismMaize Spill (유전자변형생물체 옥수수의 자생개체 발생가능성 조사)

  • Eom, Gyu-Hyeon;Jang, Yoon-Hee;Du, Xiao-Xuan;Kim, Eun-Gyeong;Park, Jae-Ryoung;Ryu, Taehun;Kim, Kyung-Min
    • Journal of Life Science
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    • v.32 no.8
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    • pp.641-646
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    • 2022
  • There are concerns about the environmental release of living modified organism (LMO) maize created to increase yields. In fact, there are cases in which LMO crops for feed have been leaked in Korea to form autoite colonies, and concerns about LMO spill are intensifying. In this study, the possibility of environmental outflow and occurrence of native organisms was analyzed using maize feed and seeds distributed in Korea. In the evaluation of the possibility of spontaneous occurrence of maize in the event of an unintentional release of maize feed made by crushing maize, the incidence rate of maize was 0.01%, which was extremely low compared to the germination rate of maize seeds. A survey of the dormant rate of maize showed that all maize seeds collected every month were dead. In the germination rate test by temperature using Daehak wax corn and Kwangpyeongok, high germination rates were found at 20℃ and 30℃, and relatively low germination rates were found at 10℃ and 40℃. In addition, all germination tests showed a higher germination rate Daehak wax corn than Kwangpyeongok. The difference between domestic and overseas cultivation maize was confirmed through a survey on the agricultural properties of three varieties of maize. The data obtained through this experiment could be the basis for the evaluation of the weediness potential of environmental risk assessment and technology to suppress the occurrence of autoite in preparation for future LMO spills.

Suppression of Powdery Mildew and Two-Spotted Spider Mite by UV-B Radiation and Mulching Type of Strawberry Cultivation in the Greenhouse (딸기 시설재배에서 UV-B 램프와 멀칭 종류에 따른 흰가루병과 점박이응애 억제)

  • Nam, Myeong Hyeon;Kim, Hyun Sook;Lee, InHa;Seo, Jeong Hak;Lee, Byung Joo
    • Research in Plant Disease
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    • v.28 no.2
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    • pp.61-68
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    • 2022
  • Powdery mildew and two-spotted spider mite are detrimental to strawberry plants and are controlled with traditional pesticides. To accommodate consumer demand, eco-friendly methods of pest control are required. Strawberries were cultivated (in soil and in a hydroponic system) for two years, and ultraviolet B (UV-B) irradiation was used as an alternative pest control during the harvesting season. Three varieties were grown (Seolhyang, Kingsberry, and Durihyang), and four UV-B lamp/mulch (black, green, and light reflection sheet [LRS]) combinations were used during harvesting: UV-B+black or green mulch, UV-B+black or green+LRS, no UV-B+black or green, and no UV-B+black or green+LRS. In all varieties, powdery mildew was 65% more controlled when UV-B irradiation was used. The adult two-spotted spider mite density was lowest in the UV-B lamp+black or green+LRS treatments. Therefore, UV-B irradiation during the strawberry harvesting season could effectively control powdery mildew and two-spotted spider mite with little side effect on the plants.

Evaluation of Potential for Antioxidant and Anti-inflammatory Material of Schisandra chinensis and Cudrania tricuspidata of Cultivated in Sunchang-gun (순창군 재배 오미자와 꾸지뽕의 항산화 및 항염증 소재 활용 가능성 평가)

  • Jeong-Ho Lee;Gyeong-Ok Jeong;Kwang-Hyeon Moon;Se-Won Lee;Seong-Hyeon Lee;Gwang-Min Lee;Yeo-Jin Yoo;Eui-Yong Lee;Hyun-Jin Tae
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.08a
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    • pp.82-82
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    • 2020
  • 염증 (Inflammation)은 물리적인 상처나 세균감염이 되었을 때 손상된 조직을 재생하고 신체를 방어하기 위해 일어나는 선천성 면역반응으로 알려져 있다. 주로 선천면역을 담당하는 대식세포는 lipopolysaccharide, reactive oxygen species와 cytokine 등에 의해 활성화되어 tumor necrosis factor-α. interleukin-1β, interleukin-6 등 염증인자들의 생성에 관여한다. 특히, 산화질소는 superoxide 음이온과 쉽게 반응하고 peroxynitrite와 같은 독성이 강한 산화제를 생성하여 단백질 및 지질의 과산화를 유도하고 세포독성을 일으키는 것으로 보고되고 있다. 따라서 본 연구에서는 항산화 및 항염증 소재를 탐색하기 위해 오미자 (Schisandra chinensis), 꾸지뽕 (Cudrania tricuspidata)을 이용하여 항산화 활성을 평가하고자 하였으며, 세포주를 활용한 세포독성 및 항염증 활성을 확인하고자 하였다. 본 연구는 전북 순창군에서 재배된 오미자, 꾸지뽕을 열 건조(60℃) 통해 건조한 후 분말화하였다. 최적 추출 조건 선정을 위해 다양한 용매 (열수, 증류수, 주정 20, 40, 60%), 온도 (25, 40, 60, 80℃) 및 시간 (1, 2, 3, 4, 5 h) 조건에서 추출된 추출물의 총 폴리페놀 함량을 비교함으로써 최적 조건을 선정하였다. 오미자와 꾸지뽕의 DPPH 및 ABTS radical 소거 활성, 총 플라보노이드 함량을 확인하여 항산화능 및 기능성 성분 함량을 평가하였다. 또한 대식세포주인 Raw 264.7을 활용하여 MTT assay, 산화질소 생성 억제 활성을 확인하여 세포독성 및 항염증 활성을 평가하였다. 실험 결과, 오미자 및 꾸지뽕은 각각 주정 40%, 60℃ 그리고 증류수, 60℃에서 추출 시 가장 높은 총 폴리페놀 함량 (약 98.3 mg GAE/g 및 88.2 mg GAE/g)을 함유하며, DPPH 라디칼 소거 활성은 약 44.6% 및 24.4%, ABTS 라디칼 소거 활성은 약 30.3% 및 40.8%로 확인되었다. 총 플라보노이드 함량은 약 21.80 mg QE/g 및 35.68 mg QE/g으로 확인되었다. 또한 오미자 및 꾸지뽕 기능성 추출물을 100 ug/mL 처리 시 세포 독성이 나타나지 않는 것으로 확인되었으며, NO 생성량을 약 56.3% 및 21.7% 저감시켜 항염증 효능을 나타내는 것으로 확인되었다.

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Comparison of Biological Activities of Extracts from Different Parts of Seabuckthorn (Hippophae rhamnoides L.) (비타민나무(Seabuckthorn, Hippophae rhamnoides L.) 부위별 추출물의 생리활성 비교)

  • Park, Yu-Hwa;Lim, Sang-Hyun;Ham, Hun-Ju;Jeong, Haet-Nim;Lee, Kwang-Jae;Kim, Kyung-Hee;Kim, Song-Mun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.7
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    • pp.975-979
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    • 2010
  • Biological activities of different parts (stems, leaves, roots, fruits) and solvents (water, ethanol) of seabuckthorn (Hippophae rhamnoides L.) grown in Korea were tested as follows. In the experiment of inhibiting $\alpha$-glucosidase activity, ethanol extract of Hippophae rhamnoides L. stem showed the highest inhibitory activity by 93% and the next highest was the ethanol extract of its leaf by 88.7%. In the case of these two extracts, the effect of inhibiting $\alpha$-glucosidase activity was extraordinarily great when comparing with control group, acarbose. In the experiment of inhibiting $\alpha$-amylase activity, water extract of leaf showed the highest result by 54.7%, among all extracts. Regarding anticancer effect for HT-29 cell and DU-145 cell, water extract of root showed 47.1% and 32.3% activities, respectively. The experiment on antibacterial activity showed that the ethanol extract from the leaf inhibitory activity of Clostridium butyricum, Proteus mirabilis, and Shigella flexneri which are the several food borne pathogenic strains. In future research, materials for biological activity appear isolated and purified and research should continue.

Studies on uptake of lead by crops and reduction of it's damage -III. Effect of water management and lime application on Pb uptake in paddy rice (농작물(農作物)에 대(對)한 납(Pb)의 흡수(吸收) 및 피해경감(被害輕減)에 관(關)한 연구(硏究) -III. 수도(水稻)의 납 흡수이행(吸收移行)에 대(對)한 물관리(管理) 및 석회물질(石灰物質)의 효과)

  • Kim, Bok-Young;Kim, Kyu-Sik;Han, Ki-Hak
    • Korean Journal of Soil Science and Fertilizer
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    • v.19 no.4
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    • pp.291-296
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    • 1986
  • A pot experiment was conducted to find out the effects of water management and application of slaked lime and wollastonite on Pb uptake of rice in a Pb added soil. The soil was adjusted to 0, 150, 300 and 600 ppm of Pb concentration. The slake lime was applied at the equivalent amount of lime requirement with 150kg/10a adding and the wollastonite, 200kg/10a, respectively. The results obtained were as follows. 1. The lead contents in leaf stem and brown rice increased with increasing the soil Pb content and the ratio of Pb/(Ca+Mg) equivalent in soil but they showed no influence on yields. 2. The application of lime and wollastonite reduced Pb content in plant. 3. The lead content in plant was higher in intermittently irrigated treatment than in submersed irrigation. 4. The soil pH was increased in the order of lime, wollastonite and control. 5. $1N-NH_4$ OAC soluble Pb content in soil was higher in the submersed irrigation than in the intermittently irrigated and was higher in wollastonite application treatment than the slaked lime after harvesting.

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Studies on Cultural Characteristics of Ganoderma lucidum (Fr.) Karst (Ganoderma lucidum(Fr.) Karst의 배양적(培養的) 특성(特性)에 관(關)한 연구(硏究))

  • Seo, Geon Sik;Shin, Gwan Chull;Park, Jong Seong
    • Korean Journal of Agricultural Science
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    • v.15 no.1
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    • pp.27-35
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    • 1988
  • In order to elucidate the systematic taxonomy and genetic characters of Canoderma lucidum, cultural characteristics of the fungus were investigated. Mycelial growth of Ganoderma lucidum were favorable on oat meal agar medium, and optimum temperature and pH of the medium for mycelial growth were $30^{\circ}C$ and 5.5-6.0 respectively. Irradiation of white fluorescent lamp inhibited mycelial growth and critical time for inhibition of mycelial growth was 4-8 hours. Concentric zones and mycelial strands of Ganoderma lucidum was induced by irradiation of white fluorescent lamp and formation of mycelial sectors was influenced by nutrient source of media and irradiation of white fluorescent lamp. These characters were different among the isolates, but no relationship was observed between these characters and the fruiting body type of the fungus. Basidiospores were formed directly from the mycelium cultured on artificial media without producing fruit body.

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