Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Suppression of Powdery Mildew and Two-Spotted Spider Mite by UV-B Radiation and Mulching Type of Strawberry Cultivation in the Greenhouse

딸기 시설재배에서 UV-B 램프와 멀칭 종류에 따른 흰가루병과 점박이응애 억제

  • 남명현 (충청남도농업기술원딸기연구소) ;
  • 김현숙 (충청남도농업기술원딸기연구소) ;
  • 이인하 (충청남도농업기술원딸기연구소) ;
  • 서정학 (충청남도농업기술원딸기연구소) ;
  • 이병주 (충청남도농업기술원딸기연구소)
  • Received : 2022.05.20
  • Accepted : 2022.06.02
  • Published : 2022.06.30
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Abstract

Powdery mildew and two-spotted spider mite are detrimental to strawberry plants and are controlled with traditional pesticides. To accommodate consumer demand, eco-friendly methods of pest control are required. Strawberries were cultivated (in soil and in a hydroponic system) for two years, and ultraviolet B (UV-B) irradiation was used as an alternative pest control during the harvesting season. Three varieties were grown (Seolhyang, Kingsberry, and Durihyang), and four UV-B lamp/mulch (black, green, and light reflection sheet [LRS]) combinations were used during harvesting: UV-B+black or green mulch, UV-B+black or green+LRS, no UV-B+black or green, and no UV-B+black or green+LRS. In all varieties, powdery mildew was 65% more controlled when UV-B irradiation was used. The adult two-spotted spider mite density was lowest in the UV-B lamp+black or green+LRS treatments. Therefore, UV-B irradiation during the strawberry harvesting season could effectively control powdery mildew and two-spotted spider mite with little side effect on the plants.

딸기에 발생하는 흰가루병과 점박이응애는 수확기 문제되는 주요 병해충이다. 딸기 수확기 병해충방제를 위해 적용 약제를 처리하지만 최근에는 소비자의 요구에 따라 친환경적 병해충 방제 방법이 요구되고 있다. 딸기 정식 후 자외선 램프(ultraviolet B, UV-B)를 이용한 친환경 병해충 방제효과를 조사하기 위해 수확기 토경과 수경재배로 2년 동안 시험을 수행하였다. 설향, 킹스베리, 두리향 품종을 정식하였으며 UV-B 램프+멀칭 종류(흑색, 녹색, 빛반사시트[light reflection sheet, LRS])별 4처리로 시험을 수행하였다. 처리는 UV-B+흑색 또는 녹색 멀칭, UV-B+흑색 또는 녹색+LRS, 무처리로 하였다. UV-B 램프 처리에 의한 흰가루병은 모든 품종에서 65% 이상의 방제효과를 보였다. 점박이응애 성충밀도는 UV-B+흑색 또는 녹색+LRS 멀칭 처리가 다른 처리보다 가장 낮았다. 따라서 딸기 수확기에 UV-B 램프 처리는 식물체에 미치는 영향도 적으며 흰가루병과 점박이응애 밀도를 낮추는 데 효과적인방제방법이될수있다.

Keywords

References

  1. Costa, L. B., Morandi, M. A. B., Stricker, S. M. and Bettiol, W. 2016. UV-B radiation reduces biocontrol ability of Clonostachys rosea against Botrytis cinerea. Biocontrol Sci. Technol. 26: 1736-1749. https://doi.org/10.1080/09583157.2016.1241981
  2. Dader, B., Moreno, A., Gwynn-Jones, D., Winters, A. and Fereres, A. 2017. Aphid orientation and performance in glasshouses under different UV-A/UV-B radiation regimes. Entomol. Exp. Appl. 163: 344-353. https://doi.org/10.1111/eea.12583
  3. Essen, L. O. and Klar, T. 2006. Light-driven DNA repair by photolyases. Cell. Mol. Life Sci. 63: 1266-1277. https://doi.org/10.1007/s00018-005-5447-y
  4. Iwasaki, Y., Sugeno, W., Goto, N., Honnma, Y., Yusa, M., Yamane, H. et al. 2019. Reconstruction support for the greenhouse strawberry production area in Miyagi Prefecture damaged by the Great East Japan Earthquake. Hortic. J. 88: 13-20. https://doi.org/10.2503/hortj.okd-si02
  5. Janisiewicz, W. J., Takeda, F., Nichols, B., Glenn, M., Jurick II, W. M. and Camp, M. J. 2016. Use of low-dose UV-C irradiation to control powdery mildew caused by Podosphaera aphanis on strawberry plants. Can. J. Plant Pathol. 38: 430-439. https://doi.org/10.1080/07060661.2016.1263807
  6. Kanto, T., Matsuura, K., Ogawa, T., Yamada, M., Ishiwata, M., Usami, T. et al. 2014. A new UV-B lighting system controls powdery mildew of strawberry. Acta Hortic. 1049: 655-660. https://doi.org/10.17660/actahortic.2014.1049.101
  7. Katayama, H., Doi, M., Furuki, T. and Mannen, J. 2020. Effect of ultraviolet-B irradiation used in combination with phytoseiid mites on the control of the two-spotted spider mite Tetranychus urticae Koch in high-bench strawberry cultivation. Annu. Rep. Kansai Plant Prot. 62: 101-106. (In Japanese) https://doi.org/10.4165/kapps.62.101
  8. Kobayashi, M., Kanto, T., Fujikawa, T., Yamada, M., Ishiwata, M., Satou, M. et al. 2014. Supplemental UV radiation controls rose powdery mildew disease under the greenhouse conditions. Environ. Control Biol. 51: 157-163. https://doi.org/10.2525/ecb.51.157
  9. Kobayashi, T., Ito, K., Yokoda, Y., Sato, T. and Yamada, M. 2019. Control of powdery mildew disease in cucumber and tomato seedlings by supplemental UV-B irradiation. Hortic. Res. 18: 65-71. (In Japanese) https://doi.org/10.2503/hrj.18.65
  10. Meyer, P., Van de Poel, B. and De Coninck, B. 2021. UV-B light and its application potential to reduce disease and pest incidence in crops. Hortic. Res. 8: 194. https://doi.org/10.1038/s41438-021-00629-5
  11. Murata, Y. and Osakabe, M. 2013. The Bunsen-Roscoe reciprocity law in ultraviolet-B-induced mortality of the two-spotted spider mite Tetranychus urticae. J. Insect Physiol. 59: 241-247. https://doi.org/10.1016/j.jinsphys.2012.11.008
  12. Murata, Y. and Osakabe, M. 2014. Factors affecting photoreactivation in UVB-irradiated herbivorous spider mite (Tetranychus urticae). Exp. Appl. Acarol. 63: 253-265. https://doi.org/10.1007/s10493-014-9773-3
  13. Murata, Y. and Osakabe, M. 2017a. Development phase-specific mortality after ultraviolet-B radiation exposure in the two-spotted spider mite. Environ. Entomol. 46: 1448-1455. https://doi.org/10.1093/ee/nvx169
  14. Murata, Y. and Osakabe, M. 2017b. Photo-enzymatic repair of UVB-induced DNA damage in the two-spotted spider mite Tetranychus urticae. Exp. Appl. Acarol. 71: 15-34. https://doi.org/10.1007/s10493-016-0100-z
  15. Nakai, K., Murata, Y. and Osakabe, M. 2018. Effects of low temperature on spider mite control by intermittent ultraviolet-B irradiation for practical use in greenhouse strawberries. Environ. Entomol. 47: 140-147. https://doi.org/10.1093/ee/nvx179
  16. Nishimura, F., Mori, M. and Satou, M. 2017. Control of powdery mildew of parsley by UV-B radiation. Annu. Rep. Kansai Plant Prot. 59: 15-20. (In Japanese) https://doi.org/10.4165/kapps.59.15
  17. Onofre, R. B., Gadoury, D. M., Stensvand, A., Bierman, A., Rea, M. and Peres, N. A. 2021. Use of ultraviolet light to suppress powdery mildew in strawberry fruit production fields. Plant Dis. 105: 2402-2409. https://doi.org/10.1094/PDIS-04-20-0781-RE
  18. Osakabe, M. 2021. Biological impact of ultraviolet-B radiation on spider mites and its application in integrated pest management. Appl. Entomol. Zool. 56: 139-155. https://doi.org/10.1007/s13355-020-00719-1
  19. Ota, E., Nishimura, F., Mori, M., Tanaka, M., Kanto, T., Hosokawa, M. et al. 2021. Up-regulation of pathogenesis-related genes in strawberry leaves treated with powdery mildew-suppressing ultraviolet irradiation. Plant Pathol. 70: 1378-1387. https://doi.org/10.1111/ppa.13384
  20. Qian, C. C., Aoki, S., Yamada, M. and Nakao, S. 2016. Influence of ultraviolet B rays on growth and reproduction of four pest thrips species (Thysanoptera: Thripidae) in a greenhouse and a natural enemy (Thysanoptera: Phlaeothripidae) of them. Jpn J. Appl. Entomol. Zool. 60: 179-188. (In Japanese) https://doi.org/10.1303/jjaez.2016.179
  21. Rural Development Administration. 2013. Manual for Strawberry Cultivation. 4th ed. Rural Development Administration, Suwon, Korea. 225 pp. (In Korean)
  22. Sakai, T. and Osakabe, M. 2010. Spectrum-specific damage and solar ultraviolet radiation avoidance in the two-spotted spider mite. Phytochem. Photobiol 86: 925-932. https://doi.org/10.1111/j.1751-1097.2010.00739.x
  23. Sakai, Y., Sudo, M. and Osakabe, M. 2012. Seasonal changes in the deleterious effects of solar ultraviolet-B radiation on eggs of the twospotted spider mite, Tetranychus urticae (Acari: Tetranychidae). Appl. Entomol. Zool. 47: 67-73. https://doi.org/10.1007/s13355-011-0090-6
  24. Short, B. D., Janisiewicz, W., Takeda, F. and Leskey, T. C. 2018. UV-C irradiation as a management tool for Tetranychus urticae on strawberries. Pest Manag. Sci. 74: 2419-2423. https://doi.org/10.1002/ps.5045
  25. Sugeno, W., Iwasaki, Y. and Hachiya, Y. 2018. Irradiation with UV-B fluorescent bulbs suppresses strawberry powdery mildew. Acta Hortic. 1227: 549-554. https://doi.org/10.17660/actahortic.2018.1227.69
  26. Sugioka, N., Kawakami, M., Hirai, N. and Osakabe, M. 2018. A pollen diet confers ultraviolet-B resistance in phytoseiid mites by providing antioxidants. Front. Ecol. Evol. 6: 133. https://doi.org/10.3389/fevo.2018.00133
  27. Suthaparan, A., Solhaug, K. A., Bjugstad, N., Gislerod, H. R., Gadoury, D. M. and Stensvand, A. 2016. Suppression of powdery mildews by UV-B: application frequency and timing, dose, reflectance, and automation. Plant Dis. 100: 1643-1650. https://doi.org/10.1094/PDIS-12-15-1440-RE
  28. Suthaparan, A., Stensvand, A., Solhaug, K. A., Torre, S., Telfer, K. H., Ruud, A. K. et al. 2014. Suppression of cucumber powdery mildew by supplemental UV-B radiation in greenhouses can be augmented or reduced by background radiation quality. Plant Dis. 98: 1349-1357. https://doi.org/10.1094/pdis-03-13-0222-re
  29. Suthaparan, A., Torre, S., Mortensen, L. M., Gislerod, H. R., Stensvand, A., Solhaug, K. A. et al. 2012. Interruption of the night period by UV-B suppresses powdery mildew of rose and cucumber. Acta Hortic. 956: 617-620. https://doi.org/10.17660/actahortic.2012.956.73
  30. Suzuki, T., Yoshioka, Y., Tsarsitalidou, O., Ntalia, V., Ohno, S., Ohyama, K. et al. 2014. An LED-based UV-B irradiation system for tiny organisms: system description and demonstration experiment to determine the hatchability of eggs from four Tetranychus spider mite species from Okinawa. J. Insect Physiol. 62: 1-10. https://doi.org/10.1016/j.jinsphys.2014.01.005
  31. Tachi, F. and Osakabe, M. 2012. Vulnerability and behavioral response to ultraviolet radiation in the components of a foliar mite prey-predator system. Naturwissenschaften 99: 1031-1038. https://doi.org/10.1007/s00114-012-0984-3
  32. Tanaka, M., Yase, J., Aoki, S., Sakurai, T., Kanto, T. and Osakabe, M. 2016. Physical control of spider mites using ultraviolet-B with light reflection sheets in greenhouse strawberries. J. Econ. Entomol. 109: 1758-1765. https://doi.org/10.1093/jee/tow096
  33. Willocquet, L., Colombet, D., Rougier, M., Fargues, J. and Clerjeau, M. 1996. Effects of radiation, especially ultraviolet B, on conidial germination and mycelial growth of grape powdery mildew. Eur. J. Plant Pathol. 102: 441-449. https://doi.org/10.1007/BF01877138
  34. Yoshioka, Y., Gotoh, T. and Suzuki, T. 2018. UV-B susceptibility and photoreactivation in embryonic development of the two-spotted spider mite, Tetranychus urticae. Exp. Appl. Acarol. 75: 155-166. https://doi.org/10.1007/s10493-018-0263-x
  35. Yuan, L., Mori, S., Haruyama, N., Hirai, N. and Osakabe, M. 2021. Strawberry pollen as a source of UV-B protection ingredients for the phytoseiid mite Neoseiulus californicus (Acari: Phytoseiidae). Pest Manag. Sci. 77: 851-859. https://doi.org/10.1002/ps.6089
  36. Yuan, L. and Osakabe, M. 2020. Dose-response and temperature dependence of the mortality of spider mite and predatory mite eggs caused by daily nighttime ultraviolet-B irradiation. Photochem. Photobiol. 96: 877-882. https://doi.org/10.1111/php.13204