• KSBB Journal
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• v.23 no.5
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• pp.431-438
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• 2008

For the experiment, to develop new materials for cosmetics, the Celosia cristata L. plant ethanol extract were used for physiological effect and cosmetics application research. The Celosia cristata L. is a Korean traditional variety grown. To investigate the effect of Ethanol extract of Celosia cristata L. on skin care, we measured anti-oxidant activity and anti-aging activity. Celosia cristata L. ethanol extract itself had anti-oxidant activity in a dose-dependent manner in 1-diphenyl-2-picryl-hydrazyl(DPPH) radical scavenging. Ethanol extract had anti-oxidant activity in a dose-dependent manner. Silica dose-dependently increased the intracellular ROS generation in RAW 264.7 cells. Celosia cristata L. ethanol extract inhibited silica-induced intracellular superoxide anion generation and $H_2O_2$ generation and hydro-peroxide generation in RAW 264.7 cells. For anti-aging effects, the hyaluronidase inhibition effects, were relatively strong and they also showed elastase activity inhibition effects, which suggesting the Celosia cristata L. ethanol extract might be used as hydration and anti-wrinkle agents. From the above results, it is referred that Celosia cristata L. ethanol extract appears to have potent anti-oxidant activity and anti-aging activity.

• Weed & Turfgrass Science
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• v.6 no.2
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• pp.151-156
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• 2017

Summer patch is the most serious disease at turfgrass field or golf course established with Kentucky bluegrass during high temperature season in Korea. Nevertheless, chemicals for the summer patch control are not yet registered in Korea. We isolated the pathogens from the turfgrass showing typical summer patch symptoms and identified as Magnaporthiopsis poae by using the internal transcribed spacer ITS1 and ITS4 sequences of rDNA. The inhibition rates of the pathogen were investigated for 10 fungicides. As results, the pathogen growth was suppressed when chemicals concentration increased and negatively correlated with incubation period with the chemicals. In triazole group, all chemicals (metconazole, myclobutanil, propiconazole and tebuconazole) treated showed the inhibition rates by 100%. Thiophanate-methyl showed the next highest inhibition effect against a summer patch pathogen. In strobilurin group, pyraclostrobin was the highest suppression effect compared with azoxystrobin and trifloxystrobin. Inhibition effect of fludioxonil and fluxapyroxad on pathogen was similar to the trifloxystrobin. Based on the results, triazole and carboxamide groups are strongly recommended due to the highest inhibition effect on the summer patch pathogen, Magnaporthiopsis poae.

• Journal of Bio-Environment Control
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• v.25 no.3
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• pp.177-183
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• 2016

The present study was conducted to establish the suppression condition of lateral root development for producing high-quality peanut sprouts. The result of Chamsiru or 6BA treatment on peanut sprouts 'Jopyeong' and 'Vietnam' showed that the higher concentration of the solutions showed the better effect for suppressing lateral root development, regardless of the kinds of lateral root inhibitor. Compared to Chamsiru, 6BA showed better effect for suppressing lateral root development, and the effect was more obvious for 'Jopyeong' than 'Vietnam'. The alternant irrigation of Chamsiru and 6BA solution could produce high-quality peanut sprouts by inhibiting bacteria activity in solution and promoting of hypocotyl and root growth, while suppressing the lateral root development. The treatment of mixture solution of $GA_3$ and Chamsiru improved the overall growth of peanut sprouts, and the effect was the highest in the treatment of '$GA_3\;50mg{\cdot}L^{-1}$ + Chamsiru $8.40mg{\cdot}L^{-1}$'.

• The Korean Journal of Mycology
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• v.19 no.1
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• pp.32-40
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• 1991

Mitochondria in Pleurotus ostreatus were isolated and purified by the stepped sucrose density gradient centrifugation, to investigate the effects of the light on the enzymatic activity of the mitochondrial ATP synthase. This enzyme, which was illuminated by the light ranging from 400 nm to 700 nm, showed that the specific activity was stimulated at 490 nm for 15 sec. Effects of organic compounds on the mitochondrial ATP synthase were also investigated at the optimum conditions; The activities of this enzyme were increased to 168 percent by the addition of 2,6-dichlo­rophenol indophenol(DCPIP), 224 percent by phenazine methosulfate(PMS), but inhibited 91 per­cent by oligomycin, 14 percent by 2-heptyl-4-hydroxyquinoline-N-oxide(HQNO) and 75 percent by 2,4-dinitrophenol (DNP), respectively. Effects of metal ions of the mitochondrial ATP synthase were investigated at the optimum conditions. The activities of the enzyme were inhibited 35 percent by $Ca^{2+}$, 14 percent by $Co^{2+}$ and 73 percent by $Mn^{2+}$. For effects of anions, the activities of this enzyme were inhibited 80 percent by $CN^{-}$, 52 percent by $SO_{4}\;^{2-}$, 28 percent by each of $CO_{3}\;^{2-}$­and $NO_{3}\;^{-}$, respectively.

• Journal of the Korean Applied Science and Technology
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• v.34 no.4
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• pp.995-1003
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• 2017

Whitening and anti-oxidant effects were observed in order to investigate the biological activations of Salvia plebeia herb ethanol extracts. No toxicity was found in both B16F10 melanoma cells and Raw 264.7 cells exposed to Salvia plebeia herb ethanol extracts for 48 hour. The extracts showed significant antioxidant activity in cell-free and cell-cultured system. In the DPPH radical assay, it removed dose-dependently DPPH radicals and showed 77.6% at $100{\mu}g/mL$. In the cells, it also significantly removed silica-induced ROS generation and LPS-induced NO production in a dose dependent manner. Using L-DOPA and L-tyrosine as a substrate, tyrosinase activity was inhibited using Salvia plebeia herb ethanol extracts in a dose-dependent manner. The supression occurred to be in the B16F10 melanoma cells, where dose-dependently inhibited Salvia plebeia herb ethanol extracts of $1{\mu}g/M$ ${\alpha}$-melanocyte stimulated hormone-induced melanin production and the inhibitory effect was 30.7% at a concentration of $100{\mu}g/mL$. This suggests that the Salvia plebeia herb ethanol extracts are usable for cosmetic product developments for anti-oxidant and whitening effects.

• Food Science and Preservation
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• v.24 no.3
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• pp.413-420
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• 2017

This study was designed to examine the in vitro antioxidant and anti-inflammatory effects of red beet (Beta vulagaris) root. Red beet root was extracted using 70% ethanol and then fractionated sequentially with n-hexane, ethyl acetate and butanol. Antioxidative ability was evaluated by bioassays using total polyphenol contents and ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid diammonium salt) radical scavenging activity. Ethyl acetate fraction of red beet root was best on total polyphenol contents ($37.02{\pm}0.37mg\;GAE/g$) and ABTS radical scavenging effects ($IC_{50}$ $42.9{\pm}9.5{\mu}g/mL$). For the anti-inflammatory activity in RAW264.7 cells, the hexane fraction showed the highest inflammatory effect. Dose response studies were performed to determine the inhibitory effect of hexane fraction of red beet root on pro-inflammatory mediators in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The hexane fraction of red beet root inhibited the NO and $PGE_2$ production and the protein level of iNOS and COX-2, and protein expression of pro-inflammatory cytokines ($TNF-{\alpha}$, IL-6 and $IL-1{\beta}$), in a dose-dependent manner. These results suggest that red beet root has considerable potential as a functional food ingredient with antioxidative and anti-inflammatory effects.

• Journal of Bio-Environment Control
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• v.4 no.2
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• pp.211-222
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• 1995

This experiments were carried out to investigate the growth responses of spinach to temperature controlling by shading treatments at different times during the summer, and the effects of environmental parameters on the growth and yield were evaluated. The fourth cultivation was showed maximum temperature at air temperature of 33.1$^{\circ}C$ and soil temperature of 28.6$^{\circ}C$, followed by third, second, fifth and first cultivation. Among the tested temperature decreasing by shading, shading with silver - coated PE film strips was the most effective in lowering greenhouse air temperatures by showing 1.1$^{\circ}C$ decrease as compared to the outside air temperature and 3.1$^{\circ}C$ decrease as compared to the air temperatures inside the non-shaded PE house and 2.3$^{\circ}C$ decrease as compared to the air temperaturese shading with black- coated PE film strips, the soil temperature was showing 2$^{\circ}C$ decrease in both shading treatments than non-shaded PE and outdoor. In first cultivation, difficult cultivated as compared to others period owing to bolting of spinach. This first period was cultivated used two domestic cultivars and six imported cultivars, significantly better growth and higher yield of spinach were recorded in glory, manchuparuk and sunlighr cultivars. The effectiveness of shading on spinach, as expressed by the crop yield increase, was the highest in the planting from July 1 to August 24.

• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.4
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• pp.315-322
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• 2008

This experiment was conducted to investigate the selection of regional double cropping system for production of organic forage in southern region of Korea. The species of forage crop used in this experiment were com, sorghum$\times$sudangrass hybrid and japanese millet for summer crops and rye and Italian. ryegrass for winter crops. In organic cultivation condition, sorghum$\times$sudangrass hybrid showed higher DM (dry matter) and TDN (total digestible nutrient) yield than that of com. Dry matter yield of com which cultivated in organic condition decreased to about 35% that of control because of weed. In winter crops, DM and TDN yield of rye is similar to that of Italian ryegrass. We could not find out the difference of nutrient value between each treatments. It means that the amount of nutrient is affected by DM productivity of each crop. The result of this study indicated that sorghum$\times$sudangrass hybrid (summer crop) and rye (winter crop), sorghum$\times$sudangrass hybrid (summer crop) and Italian ryegrass (winter crop) cropping system could be recommended as producing high yield of organic forage in southern region of Korea.

• Journal of Bio-Environment Control
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• v.10 no.4
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• pp.213-218
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• 2001

In 2000, domestic protected cultivation area was about 52,189 ha including 13,621 ha of heating greenhouses. Recently, heating cost accounts for 25 to 30% of total production cost which has been increased due to the rise of oil price, while the heating cost was about 15% in other advanced countries. To reduce the heating energy cost, the study of minimizing the heating space of greenhouse have been conducted from 1998 to 1999. The system was developed to control the heating space according to crop growth by moving horizontal curtain up and down. Installation of the heating space-control curtain in greenhouse decreased heating capacity to 264 m$^3$compared to 661.5 m$^3$in the traditional curtain, and consumpted fuel was saved about 56% point in semiforcing culture and 28% point in retarding culture of pepper. In addition, uniform distribution of air temperature and relative humidity in greenhouse environment resulted in earlier flowering and higher yields in hot pepper.

• Journal of The Korean Society of Grassland and Forage Science
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• v.27 no.4
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• pp.275-280
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• 2007

This experiment was conducted to investigate the selection of regional double cropping system for production of organic forage in middle part of Korea. The species of forage crop used in this experiment were corn, $sorghum\;{\times}\;sudangrass$ hybrid and japanese millet for summer crops and rye and Italian ryegrass for winter crops. $Sorghum\;{\times}\;sudangrass$ hybrid showed higher DM (dry matter) and TDN (total digestible nutrient) yield than that of corn. Dry matter yield of corn decreased to 56% that of control because of weed. In winter crops, DM and TDN yield of rye is higher than that of Italian ryegrass. Rye was more adequate to produce organic forage in middle part of Korea. We could not find out the difference of nutrient value between each treatments. It means that the amount of nutrient is affected by DM productivity of each crop. The result of this study indicated that $sorghum{\times}sudangrass$ hybrid (summer crop) and rye (winter crop) cropping system could be recommended as producing high yield of organic forage in middle part of Korea.