• Proceedings of the KSAR Conference
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• 2002.06a
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• pp.12-12
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• 2002

난포액은 난자의 성숙을 억제하는 성분을 함유하고 있으나, 억제성분의 화학적 성질은 연구자에 따라 다르게 보고되고 있다. 따라서 본 연구는 난자의 성축과 관련하여 제l극체의 형성을 억제하는 단백성 성분을 화학적으로 규명하기 위하여 실시하였다. 난포액 함유 난자성숙 억제 인자를 분리하기 위하여 난포액을 methanol로 추출한 다음 Superose 12 및 Superdex column 을 이 용하여 gel filtration 을 실시하였다. 회수한 Superdex 분절을 PITC로 처리한 다음, Amino Acid Analysis column을 이용, 억제 인자를 동정하였다. (중략)

• The Microorganisms and Industry
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• v.16 no.3
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• pp.20-25
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• 1990

면역계에는 조력T세포(helper T cell)와는 반대로 면역반응을 억제적으로 조절하는 것이 주 기능인 억제성 T세포(suppressor T cell)가 존배한다는 것이 아려져 있다. 이 억제성 T세포가 존재한다는 개념이 면역학및 임상의학에 준 영향은 매우 지대하여, 앨러지나 자가 면역질환 같은 질병은 억제성 T세포의 결핍으로, 그리고 면역 결핍증같은 질병은 T세포가 지나치게 활성화된 것으로 설명되기에 이르렀다. 한마디로 거의 모든 저하된 면역반응은 억제성 T세포와 연관하여 설명되었다. 그러나 억제성 T세포가 발견된지 근 20년이 되고 그에 관한 논문이 5000여편에 달하는 최근까지도 억제성 T세포 및 이들 세포가 만드는 억제성 T세포 인자에 대한 실체는 정확히 규명되지 못하고 있다. 오히려 억제성 T세포가 조력 T세포처럼 별개의 세포군으로 존재한다는 것을 부인하는 학자도 최근 나오게 되었다.(이 분야의 연구에서 나타난 문제점들이 정리되어 있음). 그러나 억제성 T세포의 존재를 부인하는 학자들도 T세포가 면역 반응의 억제에 관여한다는 사실, 적어도 그 현상 자체를 부인하지는 않았다. 다시말하면, 면역반응이 T세포에 의하여 억제되는 현상은 이미 수 천년의 논문을 통하여 입증되고 있다. 면역반응의 억제에 있어서 억제성 T세포및 억제성 T세포 인자의 역할은 앞으로의 연구를 통해 명확히 규명될 것이며, 정말 억제성 T세포가 있느냐 없느냐 하는 것은 이 총설의 주제가 아니다. 다만 여기서는 조력 T세포도 경우에 따라서는 면역반응의 억제에 관여한다는 최근의 연구결과를 간단히 소개하고자 한다.

• The Korean Journal of Zoology
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• v.38 no.3
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• pp.433-441
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• 1995

Mammary tissue-specificity of the caprine $\beta$-lactoglobulin promoter appears to be secured by repression in non-expressing cells. In order to identify the mechanism of the negative regulation, the upstream promoter sequence of the caprine $\beta$-lactoglobulin gene was analyzed in detail. The repression was mediated by the upstream flanking sequence from -47O to -205. The sequence could repress the promoter activity of $\beta$-lactoglobulin in either orientation. The effect of the putative negative regulation element of caprine $\beta$-lactoglobulin on heterlogous promoters, however, varied: the promoter activity of herpes simplex virus thimidine kinase was either repressed or activated by the sequence depending on its orientation, while the SV4O early promoter was activated rather than repressed. The regulatory sequence involving the putative negative regulatory element was strongly shifted with the nuclear extract from non-mammary HeLa and CV-1 cells, while only weak shift was observed with that of mammary HC11 cells. Such correlation between repression and factor binding suggests that the protected regions in foot-printing assay may be the negative regulatory elements of $\beta$-lactoalobulin that serve tissue-specific repression.

• 대한불안의학회:학술대회논문집
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• 2005.05a
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• pp.14-24
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• 2005

o 기질적 행동억제(behavioral inhibition)은 조건 및 비조건 공포의 기저 기전의 신경계와 관련이 있다. o 환경적 영향이 행동 억제에 영향을 미친다. o인지과정이 행동 억제의 표현에 영향을 줄 수 있다. o 행동 억제는 정신병리의 위험 인자이다.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.258-258
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• 1994

본 연구에서는 토양균이 생성하는 2차 대사산물로부터 세포증식 촉진인자 (Maturation Promoting Factor. MPF)인 cdc2/cdk2-cyclin의 복합체를 특이적으로 억제하는 물질을 분리하고 그 물질의 생리활성을 조사하였다. 토양으로부터 순수분리된 300여개의 토양균에서 생성된 배양액을 취하여 MPF의 특이적인 기질인 합성 peptide (CSH103 :HATPPKKKRK)를 사용하여 인산화 활성을 측정하였다. 그중 MPF 활성 억제능이 90％ 이상인 19개의 균주를 1차적으로 선정한 후 각각에 대하여 열/pH에 대한 안정성, 각종 용매에 대한 추출성 등 이화학적 성질을 규명하였으며 이중 균주 LPL 931로부터 MPF 활성 억제제를 분리하고자 하였다. 예비실험의 결과로부터 토양균 LPL 931을 대량배양하여 열처리하고, 비이온성 수지인Amberlite XAD-2에 결합시키고 70% acetone으로 용출시켰다. 이 추출물로부터 ethylacetate와 n-butanol을 사용하여 MPF 억제 활성물질을 추출하였다. 이 추출액을 실리카겔 관 크로마토그래피, 분취 TLC, 분취 HPL를 하여 MPF 활성 억제 분획을 분리하였다.

• Korean Journal of Food Science and Technology
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• v.30 no.5
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• pp.1000-1005
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• 1998

To establish the process control for inhibiting browning of Doenjang, the factors of browning were investigated. The browning of Doenjang was prompted by oxygen, temperature, light and Fe. Expecially temperature, oxygen and metal were main factors of browning in Doenjang. The Color Doenjang was determined by ratios of raw materials, process of manufacture and the channel of distribution. Among ascorbic acid and anti-browning agent, anti-browning agent was effective to inhibit the browning of Doenjang. The process control to inhibit the browning of Doenjang was as follows: temperature; $20^{\circ}C$ or less than, oxygen; elimination, Fe; inhibition of inflow.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.4
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• pp.587-593
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• 1998

To elucidate anti-tumor promoter from seaweed, the anti-tumor promoting activity of Ecklonia stolonifera, Undaria pinnatifida and Laminaria japonica extracts were determined by Epstein-Barr virus (EBV)-early antigen (EA) induction caused by a tumor promoter, teleocidin B-4. The methanol extracts of seaweed were subsequently fractionated with diethyl ether, distilled water, chloroform and ethyl acetate. Among the solvent fractions tested, chloroform and ethyl acetate fraction of E. stolonifera showed a high anti-tumor promoting activity at the levels of 88.0 and $85.9\%$ by the addition of 20 ${\mu}g/m{\ell}$, respectively. To characterize anti-tumor promoters from solvent fractions of E. stolonifera, the effects of phenols, chlorophyll derivatives and carotenoids on the anti-tumor promoting activity were investigated. Phenols, such as bromophenol and phloroglucinol showed anti-tumor promoting activity of $57\~66\%$ at 20 ${\mu}g/m{\ell}$. Pigments, such as chlorophylls and carotenoids exerted high anti-tumor promoting activities. Chlorophyll a and pheophorbide a exhibited the activity of $77.4\%$ and $66.6\%$ at 5${\mu}M/m{\ell}$, respectively. The active compounds of carotenoids were tentatively identified as lutein and $\alpha-cryptoxanthin$ from the profiles of visible spectra and R_f value of their authentic compounds, and showed anti-tumor promoting activities of $76.9\%$ and $84.4\%$ at dose of 20 ${\mu}g/m{\ell}$, respectively.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 1998.06a
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• pp.284-285
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• 1998

• Journal of the Korean Applied Science and Technology
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• v.37 no.3
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• pp.385-397
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• 2020

This study investigated whether swimming exercises improves obesity through regulation of angiogenesis in white adipose tissue. Female mice with high-fat diets were divided into sham-operated group (Sham), ovariectomized group (OVX), and swim-trained ovariectomized group (OVX + Swim). Compared to the Sham, OVX increased body weight, adipose tissue mass and size of adipocyte. However, these factors (: such as body weight, adipose tissue mass and size of adipocyte) of OVX + Swim decreased compared with OVX. Compared with the Sham, OVX increased the mRNA expression of angiogenic activator and MMPs and decreased the mRNA expression of angiogenic inhibitors in white adipose tissue. But OVX + Swim decreased the mRNA expression of angiogenic activator and MMPs and increased the mRNA expression of angiogenic inhibitors in white adipose tissue, compared with the OVX. Theses results suggested that swimming exercises the angiogenesis in white adipose tissue, resulting to improve obesity in high-fat diet-fed female OVX mice.

• Journal of the Korean Society of Food Science and Nutrition
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• v.44 no.1
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• pp.7-13
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• 2015

In this study, anti-inflammatory activity of hot water extract of Aronia fruits (AF-H) was examined. Pre-treatment with AF-H significantly inhibited production of nitric oxide (NO) and prostaglandin E-2 in a dose-dependent manner in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The inhibitory effect of AF-H on LPS-induced inflammation was also confirmed by down-regulation of inducible NO synthase as well as cyclooxygenase-2 protein expression. Furthermore, treatment with AF-H significantly inhibited secretion of inflammatory cytokines such as tumor-necrosis $factor-{\alpha}$ and interleukin-6. Signal transduction pathway studies further indicated that AF-H inhibited LPS-induced activation of nuclear $factor-{\kappa}B$, but not mitogen-activated protein kinase. Treatment with AF-H also partially protected against LPS-induced lethal shock in C57BL/6 mice, although its effect was not statistically significant. These results suggest that AF-H is a more promising nutraceutical or medicinal agent for inhibition of LPS-induced inflammation or inflammation-related diseases.