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http://dx.doi.org/10.3746/jkfn.2015.44.1.007

Anti-Inflammatory Effect of Hot Water Extract of Aronia Fruits in LPS-Stimulated RAW 264.7 Macrophages  

Yang, Hui (Department of Microbiology, College of Medicine, Konyang University)
Oh, Kwang-Hoon (Department of Physical Education, College of Education, Kongju National University)
Yoo, Yung Choon (Department of Microbiology, College of Medicine, Konyang University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.44, no.1, 2015 , pp. 7-13 More about this Journal
Abstract
In this study, anti-inflammatory activity of hot water extract of Aronia fruits (AF-H) was examined. Pre-treatment with AF-H significantly inhibited production of nitric oxide (NO) and prostaglandin E-2 in a dose-dependent manner in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The inhibitory effect of AF-H on LPS-induced inflammation was also confirmed by down-regulation of inducible NO synthase as well as cyclooxygenase-2 protein expression. Furthermore, treatment with AF-H significantly inhibited secretion of inflammatory cytokines such as tumor-necrosis $factor-{\alpha}$ and interleukin-6. Signal transduction pathway studies further indicated that AF-H inhibited LPS-induced activation of nuclear $factor-{\kappa}B$, but not mitogen-activated protein kinase. Treatment with AF-H also partially protected against LPS-induced lethal shock in C57BL/6 mice, although its effect was not statistically significant. These results suggest that AF-H is a more promising nutraceutical or medicinal agent for inhibition of LPS-induced inflammation or inflammation-related diseases.
Keywords
Aronia fruits; anti-inflammatory activity; nitric oxide; $TNF-{\alpha}$; $NF-{\kappa}B$;
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