• Food Science and Preservation
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• v.17 no.3
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• pp.365-369
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• 2010

This study was conducted to investigate the cultural characteristics of Paecilomyces tenuipes PJ-1 separated from the nature. We investigated the effect of media, pH and temperature in growth of Paecilomyces tenuipes PJ-1 on solid culture media and the effect of inoculum size, glass beads in liquid culture condition. Paecilomyces tenuipes showed the most favorable growth on PDA among 5 different media. The optimum growth pH and temperature were at pH 6.0 and $25^{\circ}C$ on solid culture. Mycelial growth of P. tenuipes decreased rapidly above $35^{\circ}C$ and under $15^{\circ}C$. In liquid culture, the optimum inoculum size was 10.0% and the SED and PMV value were increased with adding glass bead and glass bead size in the range of glass bead 0~50 ea and size 3, 5 mm.

• Journal of Forest and Environmental Science
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• v.14 no.1
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• pp.1-13
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• 1998

In this paper, it has been discussed on the relation between the anatomical features of woods and the penetration of methyl methacrylate monomer system into major Korean tree species, especially the Pinus koraiensis, Larix leptolepis, Quercus mongolica and Tilia amurensis. 1. There was some differences in penetration of polymers among different tree species, but generally the penetration of ring porous woods seems to be larger than that of diffuse porous woods and it was very difficult to penetrate to Larix leptolepis, especially to the that of heart wood. 2. The penetration on transeverse surface was relatively easy but radial and tangential impregnation were somewhat hard and differences between them were negligible. 3. The penetration of sapwood was larger than that of heartwood and its differences varied according to the existence of tyloses or contents in vessel. 4. Generally the polymer penetration of earlywood was larger than that of latewood in ring porous woods, but on the contrary, penetration of earlywood was small than that of latewood on diffuse porous woods. 5. Vessel diameter of wood had nearly no relation to polymers penetration. This seems to come from the reason that effective vessel diameter varies with the existence of tyloses or contents in vessel. 6. The penetration of ray tracheids was larger than that of ray parenchyma cells in softwoods, but penetration of hardwood rays seems to be small in comparison with softwood ray parenchyma cells. 7. Specific gravity and annual ring width had no relation to the penetration of polymer.

• The korean journal of orthodontics
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• v.29 no.2 s.73
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• pp.147-156
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• 1999

Skeletal Cl III malocclusion is an orthopedic appliance mainly used for growing children with maxillary undergrowth, which largely entails skeletal Cl III malocclusion. It improves anterior crossbite and maxillary position and thus, enables patients to attain favorable Profile but often involves unfavorable profile with protrusive upper and lower lips. Therefore, if orthodontists have knowledge of which condition helps obtain favorable occlusion and profile, they are able to predict the prognosis and limitation of the treatment. This study was done in order to help obtain favorable Profile after treating growing skeletal Cl III children. In the study, we classified childern into two groups, the one with favorable profile(Group 1, n=12) and the other with unfavorable profile(Group 2, n=14) and, with retrospective study using pre- and post-treatment lateral cephalogram, drew the following conclusions. 1. As patients had more serious labioversion of upper incisors, they were more unlikely to have favorable profiles after the treatment. Protrusion of prosthion, which was related with maxillary incisors, also affected profiles. 2. As the NL-ML angle before the treatment was small, it was more likely to get favorable profile. 3. As the degree of lower lip protrusion was high, it was likely to have bialveolar protrusion after the treatment. 4. As the degree of downward and backward rotation of mandible was high, it was likely to get unfavorable profile.

• Korean Journal of Medicinal Crop Science
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• v.12 no.6
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• pp.500-507
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• 2004

The establishment of an efficient protocol of plant regeneration from leaf explant cultures of Perilla spp. is reported. Regenerated shoots were obtained from leaf explant cultures on solid MS medium containing different concentrations of cytokinins and auxin. The effect of cytokonin and auxin differed depending on each acession. The combination treatments of high level of cytokinin and low level of auxin was more effective for plant regeneration in Perilla frutescens. The best concentration of sucrose was 3% for regeneration. Of spermidine, spermin and putrescine. treatments, the most effective treatment for plant regeneration was $10\;mg/{\ell}$ spermidine.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.20
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• pp.1-26
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• 1975

In order to provide basic information for the application of tissue culture to a rice breeding program. experiments were carried out on the callus formation from seedings and anthers and organ differentiation from the callus of rice varieties and their hybrids. It appeared that in general the callus tissue was more easily induced in japonicas than in indicas with significant varietal differences. A highly significant positive correlation (r=0.896$^{**}$) was obtained between the fresh weight of callus induced in NAA medium and in 2, 4-D medium in the same variety. Callus formed on the medium with 2, 4-D was more friable than that formed on the medium with NAA. The callus formation from seedlings was better than from anthers in upland rice varieties. The easiness of the callus formation appeared to be a dominant character in crosses of rice varieties. Varietal difference was also noticed in the organ differentiation from the callus and the root differentiation seemed to be easier than the shoot differentiation. Most of the plants derived from the cal1us were albinos and the frequency of occurrence of albinos was greater in callus of seedlings than in that of anthers. Greater amount of callus tissue was formed from anthers of $F_1$ plants than anyone of their parents in remote-crosses but it was clear in close-crosses. A highly significant positive correlation (r=0.504$^{**}$) was found between the callus formation from seedlings and from anthers.

• The Korean Nurse
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• v.22 no.1 s.119
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• pp.47-64
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• 1983

간호원은 비록 그가 양호교사가 아니라고 하더라도 그가 처한 위치에서 거의 매일 건강교육을 실시하고 있다고 할 수 있다. 그러한 비정규직 교육은 상황에 따라 간호활동의 대상자들에게 수시로 별 준비 없이 실시한 경우도 있겠으나 효율적인 결과를 얻을 수 있는 체계적인 보건교육은 구체적인 학습목적과 교육과정이 교육적으로 미리 계획되어 있어야만 가능하다고 할 것이다. 양호교사의 기본적인 역할이 학교 구성원들의 건강증진과 질병예방에 있으며 효율적인 건강교육을 통하여 이러한 목적을 달성한다고 할 때, 양호교사는 자신을 그 학교공동체의 주된 건강교육자로 이해하고 학교에서 이루어지는 모든 보건교육 과정에 적극적인 참여를 하여야만 한다. 그러한 역할중의 하나는 보건에 관한 수업을 실시하는 학과목 교사나 담임교사들이 건강교육에 관련한 협의를 양호교사에게 요청하도록 적극적인 건강교육환경을 학교 내에 조성하여야 하는 일과 중요한 건강주제에 대하여서는 양호교사가 직접 수업을 교실에서 이끌어야 하는 것일 것이다. 이 논문에서 다룬 보건교육 교안 연구는 양호교사에게 또 건강에 관한 주제를 가지고 수업을 하는 일반교사에게 중학생의 건강교육 과정의 한 모델로써 사용하는데 도움이 될 것으로 본다. 건강교육 프로그램을 질병예방, 건강증진에 초점을 두어 설계한다고 할 때 건강교육의 주된 방향은 대상자 하나 하나의 생활에서 건강을 위한 바람직한 행위를 생활화하도록 하는 것이다. 이 목적을 학교에서 달성하기 위해서는 건강교육 프로그램을 통하여 학생들에게 건강에 대한 지식을 전달하고 그 지식 위에 건강을 위한 바람직한 행위를 하도록 동기를 부여하며 그러한 행위를 지속하도록 보강하는 것이 필요하다고 할 것이다. 사람들은 건강에 대한 지식이나 정보를 가지게 되면, 행위는, 그것에 상응하여 바람직하게 행할 것이라고 가정하기가 쉽다. 그리하여 건강에 대한 정보를 주는 것 건강교육을 했다고 간주하기도 한다. 그러나 실제적으로 건강을 위한 정보를 받고 지식을 가졌다 하더라도 바람직한 행위를 실천하지 않거나, 습관된 반건강 행위를 바꾸지 않는 경우가 많다. 그러므로 건강한 행위를 실천하기 위해서는 그에 대한 가치관이나 태도가 먼저 분명하게 세워져야 한다. 학생들의 경우에도 그 가치관이 확립되어야 하겠으므로 이를 위한 효과적인 교육방법이 제시된 교육안 모델이 있어야 할 것이다. 즉 건강문제에 대한 태도의 확립과 그에 따른 올바른 행위를 실천하도록 하기 위하여 구체적으로 치밀하게 계획된 건강교육 안이 연구되어 지는 것이 건강교육의 가장 중요한 과제중의 하나이다. 이 연구에서 제시하는 건강교육 프로그램에서는 많은 부분의 정보가 학생들 스스로에 의해서 수집되도록 하였다. 그렇게 함으로써 그들이 흡연에 관한 사실들을 스스로 명백하게 파악할 수 있었기 때문이다. 또한 소그룹 토의와 논쟁의 방법을 통해서 학생들이 흡연에 대해서 자신들의 생각하고 있는 것을 검토하여 올바른 판단을 하도록 하는 기회를 가질 수 있도록 하였다.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.1
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• pp.30-42
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• 1986

In order to find out the best media, explants and environmental conditions for induction of calluses and organogeneses of Pinellia ternata (Thunb.) Breit in vitro, various parts of adult have been cultured on Murashige & Skoog's medium containing various levels of 2,4-dichlorophenoxy acetic acid(2,4-D) and kinetin. The results obtained were as follows: Calluses were induced from the surface of apical meristem and leaf tissue. Formation and growth of calluses in petiole ex plants were best on the MS medium complemented with 2,4-D 2.0 mg/l and kinetin 0.2mg/l. But callus formation in stem ex plants of the nearest tuber was not induced at all kinds of media. Plantlets occured at all treatment except absence of growth regulator. Their numbers, size, leaf and fresh weight were promoted by 2,4-D 2.0mg/l and kinetin 0.2mg/l. Root growth was increased on the medium containing higher 2,4-D concentrations. Size and fresh weight of callus were increased at 25$^{\circ}C$ compared with 10, 20 and 30$^{\circ}C$, respectively. Optimal pH value was at 6.0 for growth of callus. Morphological aberrations were observed in plantlets, especially in regenerated leaves. The separation of the broad leaved plantlets and albino were observed in some cultures. Growth of plantlets after transplantation was best in pots with the sterilized vermiculte. But abnormal variants withered up.

• Journal of Mushroom
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• v.3 no.2
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• pp.45-51
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• 2005

This study was carried out to obtain the basic data on artificial culture of Sparassis crispa. The mycelial growth and density were good in the PBA, PDA and PDM media, and especially prominent in the PBA medium. The optimum condition for the mycelial growth was $25^{\circ}C$ and pH 5.0, respectively. The carbon sources such as glucose, fructose, mannose, xylose(monosaccharide) and maltose(disaccharide) were favorable to mycelial growth of S crispa. The optimal concentration of glucose is 1.0~1.5%. As nitrogen sources, threonine, peptone, glycine, glutamine and valine appeared to be favorable to the mycelial growth and density. The optimal concentration of peptone is 0.3%.

• Korean Journal of Medicinal Crop Science
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• v.5 no.1
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• pp.21-27
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• 1997

This experiments were conducted to determine the effect of thidiazuron on forming tuberlets and plant regeneration of Pinellia ternata T. by tissue culture. The addition of $5\;{\mu}M$ TDZ to the medium had better regeneration than that of any other treatments of NAA and TDZ. At the combination treatments of NAA and TDZ, as the level of thidiazuron increased, the rate of shoot regeneration was incresed while the increment of NAA concentration inhibited the rate of shoot regeneration. The supplement of $5\;{\mu}M$ thidiazuron produced the best number of micro-tubers per explant and the number of micro-tuber formed was 25 in MS medium and 29 in MG medium on 30 day culture, respectively. Microtuber formation was the best on MG medium with 1.0 mg/l NAA and $5\;{\mu}M$ thidiazuron. MG medium was superior to MS and B5 medium for the growth of tuberlets. Half strength of MS medium with NAA 2 mg/l was the most effective for root formation. Rooting ability on nursery soil of plantlets produced in in vjtro was good as a 80% after 3 weeks.

• Applied Biological Chemistry
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• v.14 no.1
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• pp.99-102
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• 1971

These experiment were conducted to study the effects of various nitrogen sources and inorganic salts upon the growth of Saccharomyces rouxii T-9, and the results obtained were as follows: 1) The strain showed the most rapid growth in the case of 2.5 to 5.0% addition of peptone as an organic nitrogen source, and 1.0% addition of $(NH_4)_2HPO_4$ as an inorganic nitrogen source respectively to the media containing no NaCl. 2) Its growth was the most rapid in the case of 1.5% addition of yeast extracts as an organic nitrogen sources, and 5% addition of urea as an inorganic nitrogen source respectively to the media containing 20% of NaCl. 3) Its growth was the most rapid in the case of 1% addition of $NaNO_3$ as an inorganic salt to the media containing no NaCl, while to the media containing 20% or 26% of NaCl its growth was more rapid in the case of 5% addition of $NaNO_3$ than 1% addition. 4) Its growth was more rapid in the case of 0.5% addition of $MgSO_4\;and\;CaCl_2$ respectively to the media containing 20% of NaCl than to the media containing no NaCl, and was rapid in the case of 1% addition of $MgCl_2$ to the media containing 26% of NaCl.