• Title/Summary/Keyword: 액체-액체 추출

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Detection of Antifungal Activities from Pomegranate (석류에서 항진균성 활성의 탐색)

  • Lee, Geum Young;Park, Tae Hee;Lee, Da-In;Park, Jeong-Ro;Choi, Sang Ki
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.2
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    • pp.287-290
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    • 2015
  • Antifungal activities of pomegranate were investigated. Seventy percent ethanol extracts of seeds, peels, and whole fruits of pomegranate showed similar antifungal activities against Candida albicans in liquid media, whereas extract of whole fruit showed relatively high antifungal activity in solid media. When 70% ethanol extracts were fractionated sequentially with chloroform and ethyl acetate, ethyl acetate fraction exhibited the highest anti-fungal activities against C. albicans. Ethyl acetate fractions of whole fruits and peel portions showed at least 36% and 25% growth against C. albicans, Candida glabrata, Candida tropicalis, and Candida lusitaniae in liquid media, respectively. These results indicate that pomegranate contains antifungal compounds soluble with organic solvents.

Extraction of Caffeic Acid and Rosmarinic Acid from Zostera marina Based on Ionic Liquids and Deep Eutectic Solvent (이온성액체와 Deep Eutectic Solvent를 사용한 잘피에서 카페인산과 로즈마리산의 추출)

  • Lee, Yu Ri;Lee, Yu Jin;Row, Kyung Ho
    • Korean Chemical Engineering Research
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    • v.52 no.4
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    • pp.481-485
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    • 2014
  • The applicability of the dipping, ultrasonic-assisted, heating methods to the extraction of useful components from Zostera marina was investigated. For the increase of the extraction yield of caffeic acid and rosmarinic acid from Zostera marina, ionic liquids and DES were used as additives in the extraction solvent. The optimum extraction conditions were found: dipping extraction, methanol as extraction solvent, 0.005 g of freeze-dried material powder, 5 mL methanol, 30 min and 0.5 g of DES-3 ($Et_4NCl$ and phenol) as additive. As a result, 0.19 mg/g of caffeic acid and 8.48 mg/g of rosmarinic acid were obtained. This method is simple and sensitive, and has been applied successfully to determine the component of caffeic acid and rosmarinic acid in Zostera marina and these results indicate that DES were used as additives is more suitable than traditional extraction for the extraction of useful components from Zostera marina.

The Extraction and Derivatization of Organotins in Water Sample by Gas Chromatograph/Mass Spectrometer (기체크로마토그래프/질량분석기에 의한 물시료 중 Organotin의 추출 및 유도체 반응에 관한 연구)

  • Hong, Jee-Eun;Lee, Kang-Jin;Pyo, Hee Soo;Park, Song-Ja;Lee, Won
    • Analytical Science and Technology
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    • v.13 no.5
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    • pp.636-645
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    • 2000
  • A method is described for the determination of organotins in water samples by GC/MS. Optimized derivatization methods for ethylation and hydrogenation of organotins were surveyed according to various reaction conditions such as time, pH and concentration of reagents. The organotins were extracted with n-hexane in presence of 0.1% tropolone and hydrogenated with sodium borohydride. Extraction recoveries of organotins with hydrogenation were in the range of 61-112%. After ethylation, organotins in water samples were extracted by liquid-liquid extraction (LLE) and solid-phase extraction (SPE). Using LLE, extraction recoveries were in the range of 74-113%. The recoveries ranged from 61-97% in the case of SPE with styrene-divinylbenzene copolymers. Method detection limits of hydrogenated and ethylated organotins ranged from 0.05 to 0.5 ng/ml and from 0.02 to 0.05 ng/ml, respectively.

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Purification of Crude Protein Mixture from Panax ginseng and Hairy Root for Proteome Analysis (인삼 및 모상근의 프로테옴 분석을 위한 단백질 추출 방법)

  • 김승일;김수정;남명희;서종복;김수현;권경훈;김영환;최종순;유종신
    • Korean Journal of Plant Tissue Culture
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    • v.28 no.6
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    • pp.347-351
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    • 2001
  • Panax ginseng C.A. Meyer is a well-known Korean traditional medicine. Until now, even though major research of ginseng has been focused on the pharmacological effect, clinical application and chemical analysis of extracted secondary metabolite for several years, the physiology and gene functions of ginseng were not well known. In this research, we have developed the protein extraction methods of ginseng root and hairy root for proteome analysis in order to elucidate the gene(s) function of ginseng. Using the liquid nitrogen (equation omitted) TCA method as protein extraction method, about 660 protein spots were detected on the 2-DE gel of hairy root. Additionally, comparative analysis result of 2-DEs of ginseng root (equation omitted) hairy root suggested that proteomes of same organism could be changeable according to the culture condition, growth stages and other stimulus.

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Application of Paint Spray Gun to Atomization of Barley Tea Concentrate (이류식(二流式) 노즐을 이용(理用)한 보리차 추출액(抽出液)의 분무(噴霧)에 관(關)한 연구(硏究))

  • Lee, Jeong-Cheol;Chun, Jae-Kun
    • Korean Journal of Food Science and Technology
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    • v.15 no.4
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    • pp.348-352
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    • 1983
  • The atomization of barley tea concentrate with paint spray gun of two fluid nozzle was investigated. The physical properties of barley tea concentrate were correlated to the operating parameters; soluble solid concentration, viscosity, specific gravity and surface tension. The air to liquid feed ratios at various barley tea concentration were correlated to the air pressures applied. The diameters of sprays at various air pressures (from 164 mmHg to 564 mmHg) and soluble solid concentrations (from 3.1% to 25.2%) were also investigated.

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Removal of Benzene-Nonaqueous Phase liquid(NAPL) in Soil Tank by NAPL Swelling and Non-swelling alcohols (토양 탱크에서 흡수 알코올과 비흡수 알코올을 이용한 벤젠-비수용상액체 제거 연구)

  • Song, Chung-Hyun;Jeong, Seung-Woo
    • Journal of the Korea Organic Resources Recycling Association
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    • v.17 no.3
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    • pp.40-47
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    • 2009
  • Coinjection of alcohol and air or alcohol flooding only were evaluated with 3-D soil tank for removal of nonaqueous phase liquid (NAPL) contaminant from soil. 70%-ethanol and 40%-isopropanol were used for non-NAPL-swelling alcohol and NAPL-swelling alcohol, respectively. 729 ml-benzene was placed in the 37 liter soil tank. Alcohols were respectively injected from the injection well placed near the bottom of the tank and mobilized free phase NAPL and aqueous phases were then recovered from the extraction well placed in the upper part of the soil tank. Approximately 50% of removed NAPLs were free-phase in all experiments. The results were completely different to the previous soil column experiment results and also implied that alcohol properties did not affect the NAPL removal efficiency in the 3-D soil tank experiment. Air was also co-injected with alcohol to evaluate co-injection effects on NAPL removal. Enhanced NAPL removal effect of co-injection of 70%-ethanol and air was also found even in the 3-D soil tank evaluation. However, co-injection effect of 40%-iso-propanol and air was less apparent. This study determined that the most important parameter governing alcohol flooding for NAPL removal would be extraction capacity to recover NAPL and aqueous phase flowing in the soil. More researches are required for improving recovery efficiency of extraction well in real soil contamination conditions.

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A Study of $^{222}Rn\;and\;^{226}Ra$ Analysis in the Groundwater by LSC (액체섬광계수기에 의한 지하수중의 $^{222}Rn$$^{226}Ra$ 분석법 연구)

  • Woo, Hyung-Joo;Yoon, Yoon-Yeol;Cho, Soo-Young;Chun, Sang-Ki
    • Journal of Radiation Protection and Research
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    • v.20 no.4
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    • pp.275-283
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    • 1995
  • PERALS(Photon Electron Rejecting Alpha Liquid Scintillation) spectrometry coupled with solvent extraction method has been set up for the analysis of $^{222}Rn\;and\;^{226}Ra$ in the groundwater. This analytical method offers low background, better energy resolution and lower quenching problem than the other techniques. By the analysis of NIST SRM 4966 $^{226}Ra$ standard, the analytical accuracy and precision were found to be 3% and 1%, respectively, and the relative standard deviation of the recovery of Rn extraction between pH2 and pH10 was 7%. Detection limits of $^{222}Rn$ and $^{226}Ra$ for 10 hours counting were counted to be $0.42 pCi/{\iota}\;and\;0.016 pCi/{\iota}$, respectively. For the test analysis of $^{222}Rn\;and\;^{226}Ra$ in the graundwater, hot spring water samples of 17 regions were analyzed. The concentration of $^{222}Rn$ were in the range of $90{\sim}5200pCi/{\iota}$ and average value was $1470pCi/{\iota}\;^{226}Ra$ concentration showed a peak value of $97.9pCi/{\iota}$ in a Kangwon region, but the average value was $1.14pCi/{\iota}$ except that region.

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Studies on the Simultaneous Analysis of Organochlorine Pesticide Residues by Gas-Liquid Chromatography (I). Solvent Extraction and Cleanup of Pesticides (기체-액체 크로마토그래피에 의한 잔류 유기염소제 농약들의 동시 분석에 관한 연구 (제1보). 농약의 용매추출 및 불순물 제거)

  • Taek-Jae Kim;Song-Ja Park;Young-Sang Kim
    • Journal of the Korean Chemical Society
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    • v.29 no.5
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    • pp.496-502
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    • 1985
  • The solvent extraction and cleanup processes for the simultaneous gas-liquid cliromatographic determination of 16 kinds of organochlorine pesticide residues were investigated. The pesticides were extracted out from-various crops with the aqueous acetone solution acidified (pH < 1.5) by adding conc. $H_3PO_4$. Most of the pesticides were partitioned from the solution with petroleum ether. Evaporated the extracting solvent, the residues were dissolved in ethylether-petroleum ether (6 : 94) eluent and eluted through the Florisil column activated at 650$^{\circ}$C for 2.5hrs. The extraction efficiency was over 94% and impurities were effectively removed by the column chromatography.

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Antimicrobial Activity of Herb Extracts (허브추출물의 항균 작용)

  • Yoo, Mi-Young;Jung, Yoon-Ju;Yang, Ji-Young
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.8
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    • pp.1130-1135
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    • 2005
  • The objective of this study was to evaluate the antimicrobial activity of four herb extracts from lavender, patchouli, rosemary and eucalyptus possibly used as food preservatives. Minimum inhibitory concentration of France rosemary extracts was 29.1 mg/mL against C. albicans ATCC 10231 and 14.5 mg/mL against B. subtilis ATCC 6613 and E. coli ATCC 25922. Both of France rosemary and American lavender herbal extracts were thermally stable between 40$^{\circ}C$ and 121$^{\circ}C$ and were stable at only neutral pH. Microbial growth was repressed by adding 2.9 mg of a commercial herbal extracts in 20 mL liquid medium with France rosemary extracts and American lavender extracts.