• Journal of Gastric Cancer
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• v.5 no.3 s.19
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• pp.191-199
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• 2005

Background: Though infections of Helicobacter pylori (H. pylori) are closely associated with activation of host angiogenesis, the underlying mechanisms, as well as the strategy for its prevention, have not been identified. Here, we investigated a causal role of H. pylori infection in angiogenesis of gastric mucosa and a potent inhibitory effect of a gastric proton pump inhibitor (PPI) on the gastropathy. Materials and Methods: A comparative analysis of CD 34 expression in tissues obtained from 20 H. pylori-associated gastritis and 18 H. pylori-negative gastritis patients was performed. Expression of $HIF-1{\alpha}$ and VEGF were tested by using RT-PCR. To evaluate the direct effect of H. pylori infection on differentiation of endothelial HUVEC cells, we carried out an in vitro angiogenesis assay. Results: H. pyfori-associated gastritis tissues showed significantly higher density of $CD34^+$ blood vessels than did H. pylori-negative gastritis tissues, and the levels were well correlated with expressions of $HIF-1{\alpha}$. Conditioned media from H. pylori-infected gastric mucosal cells stimulated a tubular formation of HUVEC cells. We also found a significant inhibitory effect of PPI, an agent frequently used for H. pylori eradication, on H. pylori-induced angiogenesis. This drug effectively inhibited the phosphorylation of MAP kinase ERK1/2, which is a principal signal for H. pylori-induced angiogenesis. Conclusion: The fact that PPls can down-regulate H. pylori-induced angiogenesis suggest that anti-angiogenic treatment using PPI may be a preventive approach for H. pylori-associated carcinogenesis.

• Journal of Bio-Environment Control
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• v.28 no.4
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• pp.404-410
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• 2019

This study was performed to investigate the seed morphological characteristics and dormancy type of Adenophora triphylla var. japonica Hara that high valued medicinal crop and to select the disinfectants and light quality for germination rate improvement. The seed disinfection was carried out using distilled water (control), NaClO 4%, $H_2O_2$ 4%, and benomyl $500mg{\cdot}L^{-1}$. The light quality treatments were set to dark condition (control I), fluorescent lamp (control II), LEDs [red, blue, green, and combined RB LEDs (red:blue = 8:2, 6:4, 4:6, 2:8)] with a photoperiod of 12/12 (light/dark) and light intensity $150{\pm}10{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ photosynthetic photon flux density. Although the Adenophora triphylla var. japonica Hara seed was an underdeveloped embryo (E) and seed (S) with an embryo (E):seed (S) ratio of 0.4, it is germinated within 30 days, and seed moisture saturation was reached within 6 hours after immersion. After seed disinfection, the mold incidence rate was significantly inhibited, and the final germination rate was the highest at 87% in the benomyl seed disinfection. The final germination rate was the highest at 92% in the red light, and the mean daily germination was the lowest in the R2B8. Therefore, there is almost no dormancy in the Adenophora triphylla var. japonica Hara seed, and benomyl seed disinfectant and red light were effective in the improvement of germination rate. So it is considered to the high value of use for medicinal crop Adenophora triphylla var. japonica Hara cultivation.

• Journal of Life Science
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• v.32 no.4
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• pp.271-278
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• 2022

The detailed mechanism by which cancer upregulated gene 2 (CUG2) overexpression induces cancer stem cell-like phenotypes is not fully understood. The downregulation of FBXW7 E3 ligase, a tumor suppressor known for its proteolytic regulation of oncogenic proteins such as cyclin E, c-Myc, Notch, and Yap1, has been frequently reported in several types of tumor tissues, including those in the large intestine, cervix, and stomach. Therefore, we investigated whether FBXW7 is involved in CUG2-induced oncogenesis. In this study, the decreased expression of FBXW7 was examined in human lung adenocarcinoma A549 (A549-CUG2) and human bronchial BEAS-2B cells (BEAS-CUG2) overexpressing CUG2 and compared with control cells stably expressing an empty vector (A549-Vec or BEAS-Vec). Treatment with MG132 (a proteosome inhibitor) prevented the degradation of FBXW7 and Yap1 proteins, which are substrates of the FBXW7 E3 ligase. To address the role of Fbxw7 in the development of cancer stem cell (CSC) phenotypes, we suppressed Fbxw7 protein levels using its siRNA. We observed that decreased levels of FBXW7 enhanced cell migration, invasion, and spheroid size and number in A549-Vec and BEAS-Vec cells. The enforced expression of FBXW7 produced the opposite results in A549-CUG2 and BEAS-CUG2 cells. Furthermore, the downregulation of FBXW7 elevated the activities of EGFR, Akt, and ERK1/2 and upregulated β-catenin, Yap1, and NEK2, while the enforced expression of FBXW7 generated the opposite results. We thus propose that FBXW7 downregulation induced by CUG2 confers CSC-like phenotypes through the upregulation of both the EGFR-ERK1/2 and β-catenin-Yap1-NEK2 signaling pathways.

• Korean Journal of Heritage: History & Science
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• v.55 no.4
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• pp.138-164
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• 2022

The Neolithic shell midden in Daejuk-ri, Seosan, is distributed on the gentle slope of a low hill close to the west coast. The bedrock of the area consists mainly of schist with various mafic minerals, but shows a partial gneiss pattern. The site consists of loamy topsoil and clay loam subsoil, and the degree of siallization is relatively low. Although the pottery excavated from the shell midden shares mostly similar features, a variety of shapes and patterns coexist. The surface colors, thickness and physical properties are slightly different. The pottery can be subdivided into three types (IA, IB and II) according to the composition of the body clay, the temper and the existence of a black core. Types IA and IB are colorless mineral pottery with a non-black or black core respectively. TypeII is colored mineral pottery with a non-black core. Type I pottery also contains non-plastic colored minerals, but type II contains a large amount of biotite, chlorite, talc, amphibole, diopside and tremolite, which include a large amount of Mg and Fe. The studied pottery contains a small amount of organic matter. Considering the grain size and relatively poor sorting and roundness of the non-plastic particles, the pottery appears to be made by adding coarse non-plastic tempers for special purposes to the untreated weathered soil around the site. The three types of pottery seem to have been incompletely fired in general. While type IB has the lowest degree of oxidation, typeII shows the highest degree of redness and oxidation. It can be interpreted that these differences depend on the firing temperature and the ratio of non-plastic particles. Through a synthesis of the minerals, geochemical data and thermal history, it can be determined that the firing temperature ranged from 600 to 700℃. The pottery types of the Daejuk-ri Shell Midden have slightly different production conditions, mineral compositions, and physical properties, but have undergone similar production processes with basically the same clay materials. The clay is almost identical to the composition of the bedrock and weathered soil distributed in the Daejuk-ri area. Currently, there is an industrial complex in the area, so it is difficult to confirm the soil and geological distribution of the site. However, it is highly probable that the area around the site was self-sufficient for the clay and tempers required for the production of the Neolithic pottery. Therefore, it can be interpreted that the group that left the shell midden in Daejuk-ri lived near the site, visited the site for the purpose of collecting and processing shellfish, and discarded the broken pottery along with shells.

• Journal of the Korean Society of Radiology
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• v.82 no.5
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• pp.1196-1206
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• 2021

Purpose To construct a standard dataset of contrast-enhanced CT images of liver tumors to test the performance and safety of artificial intelligence (AI)-based algorithms for clinical decision support systems (CDSSs). Materials and Methods A consensus group of medical experts in gastrointestinal radiology from four national tertiary institutions discussed the conditions to be included in a standard dataset. Seventy-five cases of hepatocellular carcinoma, 75 cases of metastasis, and 30-50 cases of benign lesions were retrieved from each institution, and the final dataset consisted of 300 cases of hepatocellular carcinoma, 300 cases of metastasis, and 183 cases of benign lesions. Only pathologically confirmed cases of hepatocellular carcinomas and metastases were enrolled. The medical experts retrieved the medical records of the patients and manually labeled the CT images. The CT images were saved as Digital Imaging and Communications in Medicine (DICOM) files. Results The medical experts in gastrointestinal radiology constructed the standard dataset of contrast-enhanced CT images for 783 cases of liver tumors. The performance and safety of the AI algorithm can be evaluated by calculating the sensitivity and specificity for detecting and characterizing the lesions. Conclusion The constructed standard dataset can be utilized for evaluating the machine-learning-based AI algorithm for CDSS.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.7 no.3
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• pp.366-376
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• 1997

$CdGa_2Se_4$, epilayer of tetragonal type are grown on Si(100) substrate by hot wall epitaxy method. The source and substrate temperature is $580^{\circ}C$ and $420^{\circ}C$ respectively, and the thickness of the film is 3 $\mu \textrm{m}$. The crystallihe structure of epilayers were investigated by double crystal X-ray diffraction(DCXD). Hall effect on this sample was measured by the method of van der Pauw and studied on carrier density and mobility depending on temperature. From Hall data, the mobility was likely to be decreased by pizoelectric scattering in the temperature range 30 K to 200 K and by polar optical scattering in the temperature range 200 K to 293 K. In order to explore of photocurrent to darkcurrent (pc/dc), maximum allowable power dissipation (MAPD), spectral response and response time. The results indicated that for the samples annealed in Se vapor the photoconductive characteristics are best. Then we obtained the sensitivity of 0.98, the value of pc/dc of $9.62{\times}10^6$, the MAPD of 321 ㎽ and the rise and decay time of 9 ㎳ and 9.5 ㎳, respectively.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.54-55
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• 2003

해수관상용으로 그 인기도가 높은 상어류는 모두가 자연에서 채집된 후 관람용 대형수조 또는 개인용 소형수조 내에서 순치 후 관상용으로 사육되고 있다. 그러나, 자연에서 채집된 대부분의 개체는 인위적인 수조 시설 내에서의 생활에 적응하는데 실패하여 결국 거의 대부분의 개체가 짧은 기간 내에 사망하게 된다. 따라서 이 연구는 인공 종묘 생산 시설에서 해수 관상용 상어류의 종묘 확보를 목적으로 실시하였다. 우리나라 제주 연안에서 채집이 비교적 용이한 까치상어 Triakis scyllium와 별상어 Cynias manazo 2종의 상어 친어를 확보하여 인공 사육 시설에서 사육하면서 수조 내에서의 자연 교미와 자연 산란에 성공하였다. 친어와 치상어의 사육관리는 자연해수를 사용하여 15~40ton 크기의 콘크리트 수조에서 사육하면서 전갱이와 같은 생사료 또는 전갱이와 어분을 주성분으로 하는 습사료를 만들어 1일 1회 급이하였다. 까치상어의 인공 종묘는 수 차례의 사산을 거듭한 끝에 2003년 5월 18일과 6월 8일 두 차례에 걸쳐서 매우 활력이 좋은 상태의 치어가 출산하였다. 별상어도 수 차례의 사산을 거듭한 끝에 2003년 5월 25일 건강한 상태의 치어를 출산시키는데 성공하였다. 지금까지 산출된 까치상어의 출산시 평균 전장은 25.70$\pm$0.88cm 평균 체중은 58.32$\pm$4.86g이었으며, 별상어의 출산시 치어의 평균 전장은 27.26$\pm$0.57cm였고 평균 체중은 55.32$\pm$1.69g로 두 종 상어의 평균 전장과 평균 체중은 유사한 경향을 보였다. 그러나, 산출 후 사육 관리 과정에서 관찰된 까치상어와 별상어의 월간 성장 중 전장을 비교한 결과, 까치상어는 1개월 전 대비 15.06%의 성장을 보였으나, 별상어는 3.15%의 성장을 보여 까치상어 보다는 별상어의 길이 성장이 느린 것을 알 수 있었다. 까치상어와 별상어의 월간 성장 중 체중을 비교한 결과에서도 까치상어는 1개월 전 대비 34.11%의 성장을 보였으나, 별상어는 2.25%의 성장을 보여 까치상어 보다는 별상어의 체중 성장이 매우 느린 것을 알 수 있었다. 출산 관리중인 까치상어와 별상어 치어의 성비를 조사한 결과, 까치상어는 ♀:♂=1:1.28, 별상어는 ♀:♂=1:2.50으로 별상어의 암 수 성비가 매우 불균형한 것을 알 수 있었다. 그리고 까치상어와 별상어의 암ㆍ수간 성장 차이를 조사한 결과, 까치상어와 별상어의 암ㆍ수간 성장 (전장, 체장 및 체중)에는 큰 차이가 없었다. 현재, 인공 사육 시설에서 관리중인 까치상어는 64마리이며 별상어는 7마리이다. 결국 소형의 수족관내에서 해수 관상어로서 사육하기 위한 조건으로서 너무 급격한 성장을 하는 어종인 경우에는 관상 기간이 짧아져 조기 폐기하여야 하는 단점을 감안하면 까치상어 보다는 별상어의 관상가치가 높을 것으로 판단되며, 반대로 대형 관람용 수족관의 경우에서는 별상어 보다 성장이 빠르고 운동력이 활발한 까치상어를 전시관람용으로 이용할 경우 전시효과가 높을 것으로 판단된다.

• Journal of the Korean earth science society
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• v.32 no.7
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• pp.860-870
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• 2011

This study is to explore the Pedagogical Content Knowledge of beginning earth science teachers about the porphyritic texture of igneous rocks, and to suggest the teaching device that can prevent a trial and error of students in earth science instruction. We developed an interview guideline concerned with basic perception on the porphyritic texture, formation condition and formation process of porphyritic rocks, teaching and learning on porphyritic rocks for it. And data was collected from 5 beginning earth science teachers (3 high schools, 2 middle schools) through a group discussion method. In result, despite the porphyritic texture can be found at hypabyssal rocks as well as volcano rocks and plutonic rocks, most beginning earth science teachers cognized that it could be found at hypabyssal rocks only by focusing the formation depth of hypabyssal rocks. Also, the formation of porphyritic texture should be considered the factors such as cooling rate, nucleation density, growth rate, growth time, etc. However they mainly reflected the formation temperature and growth rate as it's parameter. Participants have wrongly perceived that a phenocryst necessarily differs from a groundmass on chemical composition. And they are inclined to discriminate phenocryst from groundmass through their chemical differences, instead of grain size.

• Korean journal of applied entomology
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• v.48 no.3
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• pp.361-368
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• 2009

We have investigated some biological characteristics, such as survival rate and fecundity, of Orius laevigatus and Phytoseiulus persimilis, which have been stored at 6, 8, 10, $12{\pm}1^{\circ}C$, RH $70{\pm}10%$, in dark condition. Overall, an appropriate temperature for cold storage was $10^{\circ}C$ for O. laevigatus and $8^{\circ}C$ for P. persimilis. 70% of adult O. laevigatus could survive for 36 days at $10^{\circ}C$. The O. laevigatus stored at $10^{\circ}C$ for $10{\sim}50$ days laid $37.1{\sim}120.5$ eggs. Since fresh (no storage) O. laevigatus laid 224.5 eggs, comparative fecundity of stored adults was $16.5{\sim}53.7%$ of normal fecundity. P. persimilis stored at $8^{\circ}C$ for $7{\sim}2$ days with (food eggs of Tetranychus urticae) laid $11.9{\sim}18.9$ eggs. Since fresh P. persimilis laid 26.4 eggs, comparative fecundity of stored adults was $45.1{\sim}71.6%$ of normal fecundity.

• Journal of the Korean Chemical Society
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• v.60 no.2
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• pp.118-124
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• 2016

Parabens were commonly used for preventing the growth of microorganisms as preservatives in the pharmaceutical, cosmetic and food industry. Also, parabens are known endocrine disruptors because of their estrogenic effects on human. Parabens affect the endocrine system and show adverse effect such as, genital malformations, precocious puberty and testicular cancer in young children, infants and fetuses. In this study, we developed analytical method for four parabens (methyl paraben, ethyl paraben, propyl paraben, butyl paraben) in human breast milk which frequently consumed by newborn baby. The analytes were extracted using liquid-liquid extraction (LLE) after enzyme hydrolysis with protease and lipase, then quantitative analysis was performed by liquid chromatography tandem mass spectrometry (LC-MS/MS). The method validation results were as follows; the linearity of calibration curves were excellent with coefficient of determinations (r²) higher than 0.999, the limit of detections (LODs) were 0.019~0.044 ng/mL, the accuracies were 85.3~105.9% and the precisions were lower than 10%. The average concentration ± standard deviation of parabens in ten human breast milk sample were MP 0.660 ± 0.519 ng/mL, EP 1.631 ± 2.081 ng/mL and PP 0.326 ± 0.320 ng/mL, and BP was not detected.