• KSBB Journal
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• 제8권5호
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• pp.488-494
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• 1993

본 연구에서는 캘리포니아 양귀비(Eschscholtzia californica) 현탁배양세포의 주요 이차대사산물인 benzophenanthridine alkaloid의 생성을 증가시키기 위해 생합성 전구체인 tyrosine, tyramine, dopamine 그리고 L-dopa를 exponential phase 말기에 투여하였다. 그리고 이때 yeast elicitor와 전구체를 동시에 투여한 것과 비교하여 보았다. 그 결과 적당량의 전구체 투여시 benzophenanthridine alkaloid의 생성은 대조구(무처리)에 비해 증가하였다. 그리고 elicitor와 전구체를 동시에 투여해 주었을 때 대조구(elicitor만 처리)에 비해 상당히 증가하였는데, 이것은 elicitor에 의해 유도된 이차대사 관련 효소에 의해 전구체의 biotransformation이 촉진된 것이라 생각된다. 이와 같이 전구체 투여에 의해 이차대사산물의 생성을 증가시키려 할 때 전구체만 투여하는 것보다는 촉진제를 동시에 투여함으로써 최종산물의 생성을 극대화시킬 수 있으리라 생각되며, 식물세포배양의 산업화에 유용하게 이용되리라 생각된다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2005년도 춘계학술발표회 요지
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• pp.2-6
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• 2005

질병의 예방, 노화억제, 돌연변이 억제 등 다양한 생리활성을 나타내는 안토시아닌 색소 C3G가 다량 함유되어 있는 흑자색 유색미의 성분연구를 수행한 결과 9가지의 천연화합물을 분리하였으며, 그 중 항산화 기능이 매우 뛰어난 페놀성 화합물 oryzafuran을 최초로 분리 그 구조를 확인하였다. 또한 allantoin과 같은 알칼로이드도 쌀에서는 처음으로 분리하였다. 이러한 결과를 통해 흑자색 유색미는 식용이상의 역할을 수행할 것으로 판단된다. 아울러 이러한 성분의 정량분석 방법과 합성과 관련된 메커니즘, 성분의 약리적 기능, 유전자원 평가 등 산업화를 위한 체계적인 연구가 수행되어야 할 것이며, 더 나아가 고C3G, 저단백, 저알레그렌, 고불포화지방산 등과 같은 다기능성 유색미 품종을 육성 보급함으로써 국민보건 및 농가소득에 크게 기여할 것으로 판단된다. 특히 이와 같은 성분이 주로 분포하는 미강은 도정과정을 통해 대부분 제거되기 때문에 회수하기가 용이하고, 따라서 부산물로 발생하는 흰쌀을 식품가공분야에서 이용할 수 있어 산업적으로 가치가 매우 높다고 판단된다.

• 생약학회지
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• 제35권2호통권137호
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• pp.128-133
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• 2004

Aconitum triphyllum Nakai, the Korean spontaneous perennial herb, is one of the most important materials of oriental drug Fu-zi(aconite) and has long been used as cardiotonic for arrhythmia, analgesic and antiinflammatory drug in oriental countries. Although the plant is widely used for the preparation of aconite, the composition and amount of the toxic aconitine type alkaloids have not studied so far. As the preliminary study for seasonal variation of major constituents in mother tuber and daughter tuber of the plant, the authors tried to elucidate phytochemical characteristics of $C_{19}-diterpenoid alkaloids by $^{13}C-NMR$ spectra and seasonal variation of the alkaloidal contents by high performance liquid chromatography.

• 한국약용작물학회:학술대회논문집
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• 한국약용작물학회 2011년도 추계학술발표회
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• pp.290-291
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• 2011

• 약학회지
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• 제38권6호
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• pp.786-790
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• 1994

The crude alkaloidal fraction of the root of Cynanchum caudatum Max.(Asclepiadaceae) was tested for the effects on the activities of free radical generating enzymes and the formation of lipid peroxide. Aldehyde oxidase was strongly inhibited to about 90% of the activity by treating 1.0 mg/ml of alkaloidal fraction, corresponding to competitive inhibition. Moreover, the formation of lipid peroxide which causes damage of cell membrane was reduced in proportion to the increasing alkaloid concentration. However, xanthine oxidase of which structure and function are similar to those of aldehyde oxidase was not inhibited by the alkaloidal fraction.

• 생약학회지
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• 제33권3호통권130호
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• pp.187-190
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• 2002

Three diterpene alkaloids, songorine (1), neoline (2), N-deethylneoline (3), were isolated from Kyong-Po Buja (Processed Aconitium carmichaeli, Ranunculaceae) which was used as Chinese traditional medicine with analgesic, anti-inflammatory and cardiotonic activity. Their structures were established by chemical and spectroscopic methods.

• 생약학회지
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• 제33권4호통권131호
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• pp.296-300
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• 2002

The contents of alkaloids in raw Cho O(Aconiti Ciliare Tuber) and its processed products were determined by high performance liquid chromatography. Processing methods 1 and 2 lowered the contents of alkaloids more than the method 3. Cho O processed with method 1 or 2 showed even lower alkaloid contents than commercially available Processed Aconiti Tuber Powder(PATP). The contents of aconitine and hypaconitine in MeOH extracts of Cho O treated with method 3(10 min irradiation) and mesaconitine of Cho O treated with method 3(5 min) were comparable to those of PATP.

• 약학회지
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• 제36권3호
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• pp.250-254
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• 1992

Benzo[C]phenanthidine alkaloids were found to exhibit considerably strong antileukemic activies. These alkaloids have been shown to be biosynthesized from the corresponding alkaloids throung an oxidative $C_6-N$ bond cleavage followed by recyclization between $C_6\;and\;C_{13}$ position of the protoberberine. Recently we have achieved the biomimetic transformation of protoberberine alkaloid, berberine into benzo[C]phenanthridine alkaloid, chelerythrine.

• 생약학회지
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• 제30권1호
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• pp.54-58
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• 1999

A method using coloric and spectrophotometric detection have been developed for the identification of the tertiary or quaternary alkaloids contained in Corydalis tuber and its preparations. The principle is based on the formation or decomposition of complex compounds. The complex compound of the tertiary and quaternary alkaloids have been formed by adding tetrathiocyanatocobaltate [II] ion to the test soln. Diverse crude drugs were screened using this method and the results indicated that isoquinoline, aconitine-type alkaloids in crude drugs can be readily detected. The method is simple, convenient, reproducible and applicable to the verification of the crude drug Corydalis tuber and its preparations.

• 생약학회지
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• 제4권1호
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• pp.1-7
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• 1973

Recent findings that agroclavine strongly inhibits: lactation and that ergocornine and ergonovine induce regression or inhibition of pituitary tumor growth in rats revealed new pharmacological activities in addition to the well-known activities of ergot alkaloids. Clavicipitic acid, isochanoclavine (I) and chanoclavine (II) are newly isolated alkaloids. It was already established that tryptophan, mevalonic acid and methionine are biosynthetic precursors of ergoline, a basic structure of the alkaloids, which is formed via $4-({\gamma},{\gamma}-dimethylallyl)-tryptophan$, mediated by dimethylallylpyrophosphate: tryptophan dimethylallyl transferase. Chanoclavine-I appears to be an intermediate to agroclavine which is converted to elymoclavine. Agroclavine and elymoclavine were also found to be hydroxylated by peroxidase to setoclavine and penniclavine, respectively. Elymoclavine is converted to ergotamine and lysergic acid ${\alpha}-hydroxyethylamide$, respectively. Pyruvate and alanine were found to be incorporated into the two-carbon unit of the ${\alpha}-hydroxyethyl$ moiety of the latter. Lysergylalanine is converted to ergometrine, but not to lysergic acid ${\alpha}-hydroxyethylamide$.