• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.51 no.3
• /
• pp.230-237
• /
• 2018

This study investigated the food and nutritional characteristics of alkaline-insoluble fractions (AIFs) recovered from bastard halibut Paralichthys olivaceus (BH) and skipjack tuna Katsuwonus pelamis (ST) roes using the alkaline solubilization. The moisture content of AIFs ranged from 4.8% to 12.8%, and ST provided significantly better yields (9.5 for STAIF-11 and 7.1 g/100 g roe for STAIF-12) than did BH (P<0.05). The protein content of AIFs ranged from 71.7% to 79.2%, with the highest level yielded by STAIF-11 (6.8 g/100 g roe). The crude fat content of AIFs was 10.9-14.3% and the mineral content was 0.7-3.4%. The major mineral components of AIFs were sulfur, sodium, potassium, and phosphorus. Color values showed that BHAIFs were significantly brighter than STAIFs. Total contents of essential amino acids were significantly higher in STAIFs (47.5-49.5%) than in BHAIFs. The major essential amino acids found in AIFs from both sources were Val, Leu, Lys, and Arg. Therefore, AIFs were significantly superior to whole BH roe in terms of physicochemical and nutritional status, and we identified species-specific differences between BH and ST. Protein is a major component of AIFs recovered from fish roes, which suggests that they have potential for use as a protein source.

• Journal of Life Science
• /
• v.27 no.10
• /
• pp.1161-1167
• /
• 2017

In order to find a new antimicrobial bacterium, we performed screening for antimicrobial activity of bacteria isolated from the eggs of a sea hare. The newly identified strain was designated as Phaeobacter inhibens KJ-2, based on the biochemical characterization and 16S rRNA gene sequence analysis. A colony of P. inhibens KJ-2 showed a circular and ruler-like smooth form at the edge, and a brown color. However, when maintained with a longer incubation time, its coloring was transformed into dark brown. From the result of SEM, P. inhibens KJ-2 is a bacillus which has a length of $0.8{\sim}1.0{\mu}m$ and a width of $0.4{\sim}0.6{\mu}m$. The optimal growth and antimicrobial activity were observed by shaking the culture for 24 hr at $20^{\circ}C$, which showed potent activity against pathogenic bacteria including Vibrio logei, Vibrio campbellii, Vibrio mimicus, Vibrio vulnificus, and Vibrio salmonicida. The antimicrobial activity was proportional to the amount of produced acylated homoserine lactones (AHLs). Therefore, we suggest that production of antimicrobial materials from P. inhibens KJ-2 is regulated by Quorum sensing (QS).

• Korean Journal of Food Science and Technology
• /
• v.30 no.3
• /
• pp.473-479
• /
• 1998

Identification of rice species was attempted by three side (top, side and front) images of brown rice. Nine parameters of each image were area, aspect ratio, maximum diameter, minimum diameter, perimeter, roundness and red (R), green (G) and blue (B) pixel values of an image. Forty rice samples consisted of 19 species used for the study and total 27 image characteristics for a kernel were measured. For calibration and confirmation, 105 and 20 brown rice kernels per each sample were used respectively. For best identification of rice species, 24 image characteristics were selected for discriminant analysis. Average percentages for correct identification of rice species were 84.75% and 84.93% for calibration and confirmation data set, respectively. The highest and lowest percentage for correct identification were 99.05% for Nongan and 50.63% for Hwaseung respectively in calibration data. The confirmation data showed that the correct identification of Nongan or Paalgong was 100%, while that of Hwaseung was 47.62%. The result of the study showed that three side (top, side and front) image of brown rice was not suitable for identification of rice species suggesting that additional techniques are required for better discrimination of rice species.

• Korean Journal of Plant Taxonomy
• /
• v.41 no.4
• /
• pp.299-314
• /
• 2011

The choice of molecular markers is the first step when selecting experimental plans in the field of population genetics. The popular molecular markers in population genetic studies are mainly allozyme, RAPD, RFLP, AFLP, microsatellite, SNP and ISSR. Among these, microsatellites are frequently found in nuclear, chloroplast and mitochondrial genome, showing a high level of polymorphism and nuclear microsatellites are codominant. Thus, it is a favorable molecular marker for population structure analyses and genetic diversity studies. Microsatellites are composed of tandem repeated 1~6 base pair nucleotide motifs and can be easily amplified by PCR reactions using locus specific primers. Because microsatellites have low cross-species transferability, however, they are only applicable between phylogenetically close species. In wild plants, the lack of genomic information and the high development cost of the microsatellite obstruct the wider use of microsatellites in plant population genetics research. In this review, we introduce the basis for microsatellite markers, the development process, and analytical methods as well as evolutionary models and their applications. In addition, possible genotyping errors which lead to erroneous conclusions are discussed.

• Archives of Plastic Surgery
• /
• v.37 no.4
• /
• pp.469-472
• /
• 2010

Purpose: Aplasia Cutis Congenita (ACC) is a rare disease characterized by the focal defect of the skin at birth, frequently involving scalp, but it may affect any region of the body. There are no etiology known but some conditions such as intrauterine vascular ischemia, amniotic adherences and viral infections are associated. The ideal treatment for the ACC is not known. Superficial and relatively small sized defects (< $3{\times}5\;cm$) may heal spontaneously and large defects related with risks of infection and bleeding may require aggressive surgical treatment. Hyalomatrix$^{(R)}$ is a bilayer of an esterified hyaluronan scaffold beneath a silicone membrane. It has been used as a temporary dermal substitute to cover deep thickness skin defect and has physiological functions derive from the structural role in extracellular matrix and interaction with cell surface receptor. This material has been used for the wound bed pre-treatment for skin graft to follow and especially in uncooperative patient, like a newborn, this could be a efficient and aseptic way of promoting granulation without daily irritative wound care. For this reason, using Hyalomatrix$^{(R)}$ for the treatment of ACC was preferred in this paper. Methods: We report a case of a newborn with ACC of the vertex scalp and non-ossified partial skull defect. The large sized skin and skull defect ($6{\times}6\;cm$) was found with intact dura mater. No other complications such as bleeding or abnormal neurologic sign were accompanied. Escharectomy was performed and Hyalomatrix$^{(R)}$ was applied for the protection and the induction of acute wound healing for 3 months before the split-thickness skin graft. During the 3 months period, the dressing was renewed in aseptic technique for every 3 weeks. The skin graft was achieved on the healthy granulation bed. Results: The operative procedure was uneventful without necessity of blood transfusion. Postoperative physical examination revealed no additional abnormalities. Regular wound management was performed in out-patient clinic and the grafted skin was taken completely. No other problems developed during follow-up. Conclusion: Hyalomatrix$^{(R)}$ provides protective and favorable environment for wound healing. The combination of the use of Hyalomatrix$^{(R)}$ and the skin graft will be a good alternative for the ACC patients with relatively large defect on vertex.

• Journal of Life Science
• /
• v.17 no.3 s.83
• /
• pp.328-333
• /
• 2007

Genetic diversity and population structure of eleven Liriope platyphylla (Liliaceae) populations in Korea were determined using genetic variation at 20 allozyme loci. The percent of polymorphic loci within the enzymes was 55.9%. Genetic diversity at the species level and at the population level was high(Hes = 0.178; Hep = 0.168, respectively), whereas the extent of the population divergence was relatively low ($G_{ST}$ = 0.064). $F_{IS}$, a measure of the deviation from random mating within the 11 populations, was 0.311. Total genetic diversity values ($H_T$) varied between 0.0 and 0.535, giving an average over all polymorphic loci of 0.323. The interlocus variation in within population genetic diversity ($H_S$) was high (0.305). An indirect estimate of the number of migrants per generation (Nm = 3.66) indicates that gene flow is high among Korean populations of the species. In addition, analysis of fixation indices revealed a substantial heterozygosity deficiency in some populations and at some loci. Mean genetic identity between populations was 0.988. It is highly probable that directional toward genetic uniformity in a relatively the homogenous habitat is thought to be operated among Korean populations of L. platyphylla.

• Journal of Korean Philosophical Society
• /
• v.145
• /
• pp.381-407
• /
• 2018

Gilles Deleuze presents his distinctive logic of sensation in "Francis Bacon: The Logic of Sensation" with his own approach to Bacon's paintings. What matters here is the sensation of 'haptic' that is 'one possibility of the eyes' but different from optical perception. The concept of 'haptic' has been translated into Korean variously, and Gilles Deleuze adopted this concept from Austrian art historian, Alois Riegl. Alois Riegl had used the concept of 'haptic' to mean proximate view (Nahsicht in German) as in seeing Egyptian bas-relief. Gilles Deleuze was in succession to Alois Riegl's way of thinking and used the concept of 'haptic' to discuss Bacon's paintings. By the way, Alois Riegl had adopted this concept anyway to read Egyptian bas-reliefs, but Gilles Deleuze applies this concept to paintings. Actually, this concept in Gilles Deleuze's theory of painting has more meanings than Alois Riegl'. That is to say, when we intend to understand "The Logic of Sensation" as a new logic of sensation to be represented more than just a discussion about Bacon's paintings, we would say that key word is the concept of 'haptic' being symbolic of Bacon's paintings. Yet, what kind of sensation is the 'haptic'? And, what is Deleuze's theory of sensation? I want to interpret "Francis Bacon: The Logic of Sensation" as a work being developed through his own theory of sensation, and further more I should like to draw special attention to the sensation of 'haptic' in order to make his theory of sensation with admirable clarity.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2015.05a
• /
• pp.634-634
• /
• 2015

최근 국지성 호우와 잦은 태풍으로 인한 돌발홍수가 빈번하게 발생하여 도심지에서의 호안유실과 범람으로 많은 외수침수의 피해가 발생하고 있다. 또한 기후변화에 따른 강우량의 증가와 집중호우로 인한 홍수 피해는 지속적으로 증가할 것으로 예상됨에 따라 대하천 유역을 중심으로 홍수범람예측 연구가 활발히 진행되고 있지만 대하천을 제외한 지방 중소하천의 연구가 미비한 실정이다. 이에 본 연구에서는 지방 중소하천 중 태풍과 집중호우의 영향이 많은 제주지역의 주요 하천 중의 하나인 한천 유역을 테스트베드로 선정하여 연구를 진행하였다. 한천은 강우 시에만 유출이 발생하는 건천으로, 집중호우 시 암반하상 조건, 복개, 교각 등으로 수위가 국부적으로 급격히 상승하는 경우가 있었다. 그리고 한천 하류부에는 도심이 위치하고 있어 돌발홍수 발생 시 막대한 피해가 발생한다. 이에 따라 홍수 피해를 줄이기 위한 제도화, 정책결정 등의 구조적 해결방안과 홍수 피해의 규모와 원인을 분석하는 비구조적 해결방안에 대한 연구가 시급하다. 따라서 본 연구에서는 홍수범람 등으로 인한 홍수 피해규모를 산정하여 각 정부부처 및 유관기관, 지자체에서 빠른 정책결정을 내릴 수 있는 자료를 제공하는 목적으로 제주도의 특성을 고려한 홍수범람위험도 산정 알고리즘을 개발하고자 한다. 본 연구에서는 제주 한천유역의 단면 자료와 빈도별 홍수량 자료를 이용하여 HEC-RAS 모형으로 수리학적 흐름특성 모의를 실사하였다. 모의된 결과를 바탕으로 ArcGIS 소프트웨어인 ESRI사의 ArcMap을 이용하여 빈도별 홍수위 자료와 제주지역 수치표고모형 자료를 활용한 빈도별 홍수범람지도를 산정하고, 좌안과 우안의 제방고로부터 위험도를 산정하여 홍수범람위험도를 각각 구축하였다. 구축된 결과를 이용하여 분석하고자하는 해당 빈도의 홍수위와 홍수량이 발생할 때의 피해지역을 예측하였으며, 예측된 지역과 제주시의 공시지가 자료를 중첩하여 피해지역에 대한 피해액을 산정하였다. 본 연구의 알고리즘을 적용한 2007년 태풍 '나리' 사상의 경우와 비교한 결과, '나리' 사상의 침수 흔적도와 유사한 홍수범람지도를 획득 할 수 있었으며, 모의된 유역의 하천 복개구간을 중심으로 홍수범람이 발생한다는 점과 우안보다 좌안에서의 홍수범람위험도와 피해액이 더 크게 나타난 점 등의 홍수범람 특성을 파악할 수 있었다. 본 연구에서 제시된 기법을 이용할 경우, 홍수에 의한 취약지에 대한 제방 설계 강화, 하천의 보수 정비 등 정책적 결정에 사용될 수 있을 것이며, 실시간 자료제공, 재해정보시스템 등에 적용하여 홍수범람 피해를 줄일 수 있는 기반기술이 될 것으로 사료된다.

• Korean Journal of Plant Taxonomy
• /
• v.46 no.4
• /
• pp.361-370
• /
• 2016

An area comprising Juwangsan National Park and its adjacent mountains (southeastern Korean Peninsula) is a good model system for testing the effects of physical barriers to gene flows in plant populations. We predicted that plant species consisting of isolated populations are genetically more differentiated than those that are rather continuously distributed. Most populations of Sedum polytrichoides occur in four isolated valleys, and we assessed the genetic variability and structures using twelve allozyme loci in ten populations. We also compared the present results to earlier findings pertaining to the two co-occurring herbs Hylotelephium ussuriense (${\equiv}$ Sedum ussuriense) (growing only in the four isolated valleys) and S. kamtschaticum (rather continuously distributed). We found moderate levels of within-population genetic variation in S. polytrichoides ($H_{e}=0.112$). Estimates of among-population divergence in S. polytrichoides were also moderate ($F_{ST}=0.250$) and, as expected, very similar to that of H. ussuriense (0.261) but considerably higher than the variation in S. kamtschaticum (0.165). An analysis of molecular variance (AMOVA) revealed that S. polytrichoides and H. ussuriense had higher percentages of among-valley variation (19% each) than S. kamtschaticum (4%). Most of this variation, as also indicated by the STRUCTURE program, was due to differences in genetic profiles between the two central valleys. We concluded that the genetic differences observed between species (S. kamtschaticum vs. S. polytrichoides and H. ussuriense) are mainly due to differences in their distribution within the study area.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2019.04a
• /
• pp.85-85
• /
• 2019

가는잎향유[Elsholtzia angustifolia (Loes.) Kitag.]는 꽃이 원줄기와 가지 끝에 조밀하고 아름답게 달리며, 전초에 함유된 정유성분의 향이 우수하여 관상 및 약용으로 이용되고 있다. 이에 본 연구는 관상소재로 이용가능한 가는잎향유의 분화 재배기술을 확립하기 위해 2018년 7월 2일에 유묘(4월 3일~5월 31일 육묘)를 포트에 정식하여 10월 15일까지 수행하였다. 대조구는 플라스틱 화분 10호에 원예상토를 충진한 다음 200구 트레이에 셀당 4립 파종하여 생산된 유묘를 정식하여 이용하였다. 기비처리는 싱싱코트를 0, 10 및 20알로 처리하였고, 추비처리는 정식 후 4주 간격으로 hyponex (4-6-6)를 0, 1000 및 $2000mg{\cdot}L-1$로 3회 엽면시비 하였다. 차광정도 실험은 0, 55 및 75%로 광량을 달리하여 수행하였다. 왜화제는 diniconazole과 daminozide를 각 0, 1000 및 $2000mg{\cdot}L-1$로 정식 후 4주차에 처리하였다. 연구의 결과, 기비와 추비 처리는 무처리와 비교하여 생육에 큰 차이가 없었으나, 절간장은 처리량이 증가할수록 억제되었으며, 처리시 무처리에 비해 유의적으로 감소하였다. 측지수 및 엽수는 기비 처리에 따른 큰 차이가 없었으며, 추비는 측지수와 엽수가 많아지는 경향이었다. 한편, 기비와 추비 처리는 개화율을 비교적 감소시키는 결과를 보였다. 차광정도가 증가할수록 초장, 절간장, 경직경, 화서수, 화서장이 감소하는 경향이 나타났으며, 무차광과 차광처리 간 측지수와 엽수는 유의적인 차이가 나타나지 않았다. 왜화제 처리는 무처리와 비교하여 절간장을 감소시켰다. Daminozide 처리시 측지수와 엽수는 무처리에 비해 증가하였으나 유의적인 차이는 없었고, 지상부의 생체중은 엽수와 측지수에 비례하여 무거워지는 결과를 보였다. 또한, $2000mg{\cdot}L-1$ 농도 처리시 개화율이 100%에 도달하였다. Diniconazole 처리는 무처리와 비교하여 큰 차이가 없었다. 따라서, 가는잎향유를 분화재배를 하기 위해서는 기비와 추비는 처리하지 않고, 무차광 조건에서 재배하는 것이 좋다. 또한, 왜화제는 daminozide $2000mg{\cdot}L-1$로 처리하는 것이 측지수, 엽수 및 개화율이 우수한 결과를 보여 분화 재배에 적합할 것으로 판단된다.