• Journal of Korean Ophthalmic Optics Society
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• v.9 no.2
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• pp.333-343
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• 2004

Impression cytology refers to application of cellulose acetate filter material to the ocular surface to remove the superficial layers of the conjunctival epithelium. The technique is non-invasive, is easy to perform, causes minimal discomfort to the patient, and can be used to follow changes in the conjunctival ocular surface over time. With this method, the morphology of the conjunctival ocular surface can be studied and the degree of squmaous metaplasia assessed. This study was performed to evaluate the conjunctival surface by impression cytology in dry eye patients. A total of 70 students with no contact lens wearing history were recruited. Subjects were required to fill in a McMonnies dry eye symptom questionnaire. The non-invasive tear thinning time(TIT) test of each subject was measured, followed by Schirmer tear test(STI), tear film break-up time(TBUT) tests and Rose-bengal staining were performed as a baseline. Conjunctival epithelial cells from the inferior bulbar conjunctiva were harvested by the impression cytology technique. The specimens collected were labelled and stained with PAS(Periodic Acid Schift)-haematoxylin. The goblet cells and conjunctival epithelial cells were observed under a light microscope of 400x magnification. The specimens were classified according to the Nelson Grading scale which was based on the degree of squamous metaplasia such as changes of goblet cells density, size/form, N:C(nucleus : cytoplasm) ratio. Dry eye patients were observed morphological changes of the epithelial cells, different nuclear alterations, decrease of the goblet cells density. The degree of cytological changes was related to severity of dry eye conditions. When the epithelial cell morphology was graded according to the system described by Nelson, specimens from the control group revealed 91.43% of the eyes to be grade 0 and 8.57% to be grade 1, whereas of the dry eye patients, 20% were grade 0, 42.86% grade 1, 34.29% grade 2 and 2,86% grade 3. Impression cytology represents a non- or minimally invasive biopsy of the ocular surface epithelium with no side effects or contraindications. It has demonstrated to be a useful diagnostic aid for a wide variety of processes involving the ocular surface. This technique is a safe, simple method and may help increase understanding of various ocular surface alterations in dry eye patients.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.4
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• pp.308-314
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• 2011

Seed germination and dormancy of three green manure seeds such as hairy vetch, crimson clover and narrow-leaved vetch (Vicia angustifolia L.) produced in Milyang, southern part of Korea were investigated to provide a basic information on the stability of seedling establishment in green manure seeds. In addition, the effect of seed storage duration on the germinability of imported hairy vetch and crimson seeds was also investigated. To determine the seed maturity time of three green manure seeds, the seeds were harvested manually at May 25, May 30, and June 4, and the 100-seed weight, germinability and percentage of hard seed were evaluated. Freshly harvested seeds of three green manure crops were strongly dormant because of hard seed coat. Germination percentage of the three green manure crops varied depending on the seed harvest time ranging from 30 - 52% in hairy vetch, 16 - 35% in crimson clover and 2 - 61% in V. angustifolia, respectively. Of the three crops, crimson clover and V. angustifolia matured early on May 30 to June 4 (around 35 to 40 days after flower) but hairy vetch seed did not mature until June 4 based on the seed germinability. The matured seed of crimson clover and V. angustifolia germinated less than 20% while scarification on seed coat significantly overcome the innate dormancy. On the other hand, the seed germination of hairy vetch and crimson clover was lower under dark than in the presence of light. The germination percentage of the imported hairy vetch and crimson clover seeds showed high with 77 - 79%, 94 - 95%, respectively, but the seed germination significantly declined by 21 - 32%, 30 - 40% after one-year of storage under natural conditions. The seeds germinated only 8 - 13% for hairy vetch but crimson clover did not germinate at all after two years storage. These results indicate V. angustifolia and crimson clover mature at May 30 to June 4 but hairy vetch did not. The imported hairy vetch and crimson clover seeds should be used within one-year after import for stable seedling establishment in green manure-rice cultivation cropping system.

• Journal of Bio-Environment Control
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• v.9 no.4
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• pp.193-200
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• 2000

The object of this study is to investigate the quality change of sweet basil grown with selenium(Se) in hydroponic culture. Sweet basil was cultured with 1 fold herb nutrient solution as suggested by European vegetable R & D Center in Belgium. Before three weeks harvest, sodium selenate(N $a_2$Se $O_4$) was supplied to 2 and 4 mg. $L^{-1}$ in the nutrient solution. Sweet basil was stored at 1$0^{\circ}C$ using 40um ceramic film and PET (polyethylene terephalate) for 15 days in modified atmosphere(MA) storage condition. The weight loss of sweet basil was higher in non-treatment compared to Se treatments in both of two films but it was decreased over 5% in PET treatment. Se concentrations in leaf tissues increased in the response to the treated levels of N $a_2$Se $O_4$concentrations, and this tendency was appeared similar results after storage. There was no significant effect of packing materials on volatilization of Se in sweet basil. The total chlorophyll and essential oil content was increased with increasing N $a_2$Se $O_4$concentration in nutrient solution. The amount of volatilization flavor was not higher at N $a_2$Se $O_4$4mg. $L^{-1}$ treatment compare to others during storage. Se content was 112.73 ug. $g^{-1}$ dry mass at 2 mg. $L^{-1}$ treatment before storage and the decrease of Se content was observed by 50% at 15 days after storage. The condition, which N $a_2$Se $O_4$2mg. $L^{-1}$$^{plement}$ in nutrient solution during growth stage and stored with 40um ceramic film on 1$0^{\circ}C$are acceptable for maintaining of sweet basil quality. Moreover it can be a proper Se concentration for human health. Overall, Se treatment in nutrient solution has effect on promoting and maintaining quality of herb during storage life. Also, there was not significant change of essential oil compounds by volatilization of Se.mpounds by volatilization of Se.

• Journal of the Korea Concrete Institute
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• v.25 no.3
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• pp.321-329
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• 2013

Recently, the medium-low level radioactive waste from nuclear power plant must be transported from temporary storage to the final repository. Medium-low level radioactive waste, which is composed mainly of the liquid ion exchange resin, has been consolidated with cementitious material in the plastic or iron container. Since cementitious material is brittle, it would generate cracks by impact load during transportation, signifying leakage of radioactive ray. In order to design the safety transporting equipment, there is a need to check the compressive strength of the current waste. However, because it is impossible to measure strength by direct method due to leakage of radioactive ray, we will estimate the strength indirectly by the dynamic modulus of elasticity. Therefore, it must be identified the relationship between of strength and dynamic modulus of elasticity. According to the waste acceptance criteria, the compressive strength of cement based solid is defined as more than 3.44 MPa (500 psi). Compressive strength of the present solid is likely to be significantly higher than this baseline because of continuous hydration of cement during long period. On this background, we have tried to produce the specimens of the 28 day's compressive strength of 3 to 30 MPa having the same material composition as the solid product for the medium-low level radioactive waste, and analyze the relationship between the strength and the dynamic modulus of elasticity. By controling the addition rates of AE agent, we made the mixture containing the ion exchange resin and showing the target compressive strength (3~30 MPa). The dynamic modulus of elasticity of this mixtures is 4.1~10.2 GPa, about 20 GPa lower in the equivalent compressive strength level than that of ordinary concrete, and increasing the discrepancy according to increase strength. The compressive strength and the dynamic modulus of elasticity show the liner relationship.

• Journal of Bio-Environment Control
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• v.24 no.4
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• pp.296-300
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• 2015

This study was conducted to investigate optimal crop loads of 'Jinok' grape for unheated plastic house culture. The crop loads of 'Jinok' grapes were managed to be 1.3, 1.8, 2.2, 2.4, and 2.6t per 10a from 2012 to 2014. We measured vine growth and berry qualities. Crop loads were not significantly affected on plant height, trunk diameter, shoot length, and the internode length of 'Jinok'. However, the maturation of berries was delayed when the crop load was higher. And the harvest date was earlier about three weeks in an unheated plastic house compared to in an open field. The average berry weight was decreased by the higher crop load although higher crop loads made higher yields showing the lowest weight at 342g with the treatment of 2.6t per 10a and the highest weight at 363g with the treatment of 1.3t per 10a. Also, the soluble solids content showed a tendency that higher crop loads brought to lower degree Brix. The contents of P, K, Ca, and Mg in grape leaves and shoots were not significantly different by crop loads. To sum up, when crop loads were under the 2.4t per 10a, the berries were harvested as a marketable fruit having $15^{\circ}Brix$ in the cultivar 'Jinok' grape. This result could help to increase grower's benefit having improved quality of fruit for the sustainable production by the established cultivation techniques for the newly developed cultivar 'Jinok'.

• Journal of agricultural medicine and community health
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• v.30 no.3
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• pp.279-292
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• 2005

Objectives: The purpose of this study is to analyze agricultural accident and disease using statistical data and materials about National Survey for Health & Nutrition in 2001 and to provide fundamental materials for studies about farmers' health and safety, decision of priority about research and policy. Results: Diagnosed chronic disease prevalence is 72.4% in farmer/fisher group, 49.8% in non farmer/fisher group. The chronic disease prevalence of musculoskeletal disease, circulatory disease, and gastroenteric disease is 46.5%, 18.2%, and 17.9% in farmer/fisher group respectively. The prevalence of musculoskeletal disease in farmer/fisher is 2.4 times higher than non farmer/fisher. This result shows that it need to evaluation for risk factors of musculoskeletal disease preferentially. Lifetime accident/poisoning rate is 18.2% In farmer/fisher group and 13.3% in non farmer/fisher group. The types of accidents were fracture>sprain>contusion and the reasons of accidents were traffic accident>falling/sliding. Conclusions: "Bad or very bad" response of farmer/fisher is almost 2 times higher than non farmer/fisher group. The rate of smoking and no exercising in farmer/fisher group is higher than non farmer/fisher group.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.44 no.3
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• pp.249-258
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• 2018

Airborne pollution causes oxidative damage, inflammation, and premature aging of skin. Resveratrol is a polyphenol compound that has various biological activities such as antioxidant, anti-inflammation, and anti-melanogenic activities but it is unstable to heat and light. Resveratryl triacetate (RTA) is a new cosmetic ingredient that is more stable than resveratrol and its skin safety and whitening efficacy have been reported previously. The purpose of this study was to examine the effects of resveratrol and resveratryl triacetate (RTA) on the inflammatory responses of human epidermal keratinocytes (HEKs) exposed to airborne particulate matters with a diameter of < $10{\mu}m$ (PM10). Cultured HEKs were exposed to PM10 in the absence or presence of resveratrol and RTA. Assays were undertaken to determine cell viability, the production of reactive oxygen species (ROS), and the expression of inflammatory cytokines. PM10 treatment decreased cell viability, and increased the expression of pro-inflammatory cytokines such as tumor necrosis $factor-{\alpha}$ ($TNF-{\alpha}$), $interleukin-1{\beta}$ ($IL-1{\beta}$), interleukin-6 (IL-6), and interleukin-8 (IL-8). Resveratrol and RTA reduced cell death and ROS production induced by PM10. PM10-induced mRNA expression of the inflammatory cytokines was either attenuated (IL-6), or enhanced ($IL-1{\beta}$), or unaffected ($TNF-{\alpha}$ and IL-8) by resveratrol and RTA. PM10-induced IL-6 protein expression was attenuated by resveratrol and RTA. This study suggests that resveratrol and RTA have activities regulating cell damage and inflammatory responses of the skin exposed to airborne particulate matters.

• Journal of the Korean Society of Food Science and Nutrition
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• v.46 no.9
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• pp.1106-1113
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• 2017

The objective of this study was to determine the quality characteristics of fried yakgwa and baked yakgwa prepared with different amounts of P. frutescens powder. Moisture contents of fried yakgwa were 7.05%, and moisture content of baked yakgwa with different amounts of P. frutescens powder were 12.42~10.44% and decreased with higher amount of P. frutescens powder. This result can be attributed to loss of water in yakgwa in the course of the fried process. Although the degree of expansion of baked yakgwa was lower than that of fried yakgwa, size and shape of yakgwa were maintained. Yakgwa is appropriate as a cookie type product. Crude lipid contents of fried yakgwa were higher than those of baked yakgwa due to the exchange reaction of water and fat during the fried process. Energy of fried yakgwa was 501 kcal and was higher than that of baked yakgwa with different amounts of P. frutescens powder. This greatly affected the fat content of each sample. Energy of baked yakgwa increased with higher amount of P. frutescens powder due to the characteristics of the ingredients or jasoyeop. For chromaticity determination, L values of fried yakgwa were lower, but a, and b values were higher than those of baked yakgwa, and L, a, and b values decreased when P. frutescens powder increased. Texture measurement showed that hardness, cohesiveness, chewiness, gumminess increased with higher amounts of P. frutescens powder, whereas springiness decreased. The antioxidant activities of fried yakgwa measured based on DPPH scavenging activity were higher than those of baked yakgwa with 0%~0.2% P. frutescens powder and lower than those of baked yakgwa with 0.3%~0.4% P. frutescens powder. In the sensory evaluation, baked yakgwa with 0.1% addition of P. frutescens powder showed the highest preference in terms of overall acceptance, and 0% addition of P. frutescens powder showed the highest preference in terms of color and flavor. These results suggest that P. frutescens powder may be a useful ingredient in baked yakgwa to improve quality and sensory properties.

• Journal of Nutrition and Health
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• v.43 no.4
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• pp.382-394
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• 2010

The purpose of this study was to evaluate the effects of a nutrition education program as part of health promoting projects in an elementary school. A total of 168 students in the 4th grade were enrolled in the program for 6 months. A self-administered questionnaire was developed to assess nutrition knowledge and dietary habit. Nutrient intakes and body shape perceptions were measured using 24-hour recall method and self-ratings of body shape figures, respectively. The education program was conducted in various ways, focused on healthy dietary behavior, nutrients' roles and sources, and proper body shape perceptions, etc. After the education program, nutrition knowledge and dietary habit were significantly improved. Dietary habit scores were significantly and positively related to nutrition knowledge levels. Body shape perceptions were significantly and properly changed after the program. Dissatisfaction with perceived current body shape and discrepancy between shapes desired and considered healthy were significantly decreased. But girls still indicated a desire to be leaner than shapes which were perceived current and considered healthy. Nutrient intakes were partly improved by the program, but some nutrients, such as calcium, folate, zinc, sodium and niacin, were still remained deficient or excessive. These findings indicate that enhancement of nutrition knowledge is extended to the improvement of dietary habit. In addition, establishment of proper body shape perception is closely related to healthy dietary habits and is required to good nutrition and health.

• Journal of Korean Society for Geospatial Information Science
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• v.22 no.3
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• pp.57-69
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• 2014

As of 2013, Korean population density is 505 persons per $1km^2$ and is ranked 3rd place in the most densely populated countries exception of city-states. It shows clearly the population is concentrated in the city area. To fulfil this urban concentration population demand, the enlargement and complexation of buildings, subway and other underground spaces connection tendency has been intensified, and it is need to construct the indoor spatial information DB as well as the accurate indoor surveying DB to promote people's safety and social welfare. In this study, Sadang station and Incheon National Airport were aimed for the construction of Wi-Fi AP location DB and RadioMap DB by collecting the indoor AP raw datas by using mobile device and those collected results were ran through the process of verification, supplementation, and analyzation. To evaluate the performance of constructed DB, 10 points in Incheon Airport- 3rd flr in block A, and 9 points in Sadang station-B1 were selected and calculated the estimated points and ran evaluation experiment using survey positioning error, which is distance between real position and the estimated position. The result shows that Incheon international airport's average and standard deviation was separately 17.81m, 17.79m and Sadang station's average and standard deviation was separately 22.64m, 23.74m. In Sadang station's case, the areas near the exit has low performance of surveying position due to fewer visible AP points than other areas. As total datas were examined except those position, it was verified that the user's location was mapping close position in surveying positioning by using constructed DB. It means that constructed DB contains correct Wi-Fi AP locations and radio wave patterns in object region, so it is considered that the indoor spatial information service based on constructed DB would be available.