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Quantitative Analysis of Transient Expression in Tah Tasai Chinese Cabbage (Brassica campestris var. narinosa) Seedlings Following Agrobacterium-Mediated Transformation (다채 (Brassica campestris var. narinosa) 유묘의 형질전환 및 일시발현의 정량적 분석)

  • Shin Dong-Il;Park Hee-Sung
    • Journal of Plant Biotechnology
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    • v.32 no.4
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    • pp.275-279
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    • 2005
  • Tah tasai chinese cabbage (Brassica campestris var. narinosa), a vegetable plant popularly consumed as several-days-old seedlings in oriental countries, can be easily cultivated using a simple appliance. We demonstrated that Agrobacterium-mediated transformation via vacuum infiltration (agroinfiltration) resulted in a successful transient GUS gene expression in tah tasai chinese cabbage seedlings. Pre-germinated seeds were found to be more susceptible to Agrobacterium infection than one-day-old or two-days-old seedlings. We also demonstrated that hydrogen peroxide (HPO) treatment increased GUS expression especially for two-days-old seedlings. In ELISA using seedlings transformed with hepatitis B surface antigen (HBsAg) DNA by agroinfiltration, HBsAg protein synthesis increased more than two folds by HPO treatment to two-days-old seedlings in comparison to the mock-treated pre-germinated seeds.

Effects of Young Sprouts Cutting Times and Nitrogen Split Application on Growth and Yield of Atractylodes japonica KOIDZ (삽주의 어린순 채취(採取)회수 및 질소분시(窒素分施)가 생육(生育)과 수양(收量)에 미치는 영향)

  • Jang, Kye-Hyun;An, Dong-Chun;Kim, Dong-Kil
    • Korean Journal of Medicinal Crop Science
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    • v.4 no.3
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    • pp.241-246
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    • 1996
  • This Experiment was conducted to know effect of cutting times and nitrogen split application rates on shoot and root-related characters of Atractylodes japonica. The cutting time was forced on none, once and twice along with three levels of nitrogen split application, all basic 50 -30 -20% and 40 -30 -30%. The plant height declined with increased cutting time and dry matter weight was increased with nitrogen split application under none and once cutting. Average yield of cutted shoot as edible wild plant was 257kg/10a at once cutting condition, but twice cutting condition was little and unfavorable to use edible wild plant because shoot was coarse. Yield 0 dry rhizome was the highest at once shoot cutting und­er 50 -30 -20% nitrogen split application by 471kg/10a.Especially, culture of Atractylodes japonka needed once shoot cutting one the 10th-30th of May.

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THE LONG-TERM GROWTH OF HOMOGENEOUS EMBRYO TOOTH GERM TRANSPLANTED INTO THE MAXILLA OF A RAT (흰쥐의 악골에 동종 이식한 태아 치아싹의 장기간 발육)

  • Ko, Dong-Hyun;Chung, Han-Sung;Kim, Seong-Oh;Lee, Jae-Ho;Choi, Hyung-Jun;Choi, Byung-Jai
    • Journal of the korean academy of Pediatric Dentistry
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    • v.34 no.1
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    • pp.53-61
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    • 2007
  • In case of missing tooth caused by dental caries or periodontal disease, it can be restored by various methods, and there has been much interest in implant and tooth transplantation. The success of tooth transplantation is going to be attained through the knowledge of growth, development and calcification of tooth. Tooth transplantation has been experimented in vivo and in vitro. Many animals such as rats, mice, cats and dogs are used for tooth transplantation experiment in vivo. In most experiments, tooth was transplanted into the extraoral site, but rare into the intraoral site In this study, to observe the capacity of formation and mineralization of tooth germ, first molar of a matured white rat was extracted and the cap stage tooth germ of a 13.5 Embryonic day embryo rat was transplanted into the extracted socket. The rats were killed 6 months later and the radiographical and histological results are as followings. 1. Tooth germ transplanted for 2 and 6 months are developing calcified tooth material such as dentin, cementum, pulp tissue, and epithelium around enamel space in the maxilla was seen. 2. The epithelium around enamel space was located beneath the oral epithelium and contained connective tissue and periodontal ligament. 3. Tooth formation was progressed as transplantation period but the size of newly formed tooth was small and the shape of tooth was incomplete.

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Antioxidation and Antigenotoxic Effects of Buckwheat Sprout Extracts (메밀싹 추출물의 항산화 효과 및 유전독성억제 효과)

  • Kim, Su-Hyun;Lee, Eue-Yong;Ham, Seung-Si
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.8
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    • pp.955-959
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    • 2007
  • This study was carried out to determine the antioxidative and antigenotoxic effects of buckwheat (Fagopyrum esculentum Moench) sprout using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical donating method and micronucleus test. Buckwheat sprout were extracted with 70% ethanol and then further fractionated to n-hexane, chloroform, ethyl acetate (EtOAc), butanol and water. Among the five fractions, the EtOAc fraction showed the highest electron donating activity ($RC_{50}$ 26.1 ${\mu}g/mL$). The effects of buckwheat sprout extracts on the frequencies of micronucleated polychromatic erythrocytes (MNPCEs) induced by MNNG (N-methyl-N'-nitro-N-nitrosoguanidine) were investigated in the bone marrow. 10, 20, 40 and 80 mg/kg of each extract were administered to animals immediately after injection of MNNG and the exposure time was 36 hrs. Inhibition effects of buckwheat sprout ethanol extract were 23.4%, 40.6%, 56.3% and 73.4%, respectively. When the fraction of hexane, chloroform, ethyl acetate, butanol and water from 70% ethanol extract were treated with concentration of 80 mg/kg, the suppression rates of the MNPCE were 64.1, 67.9, 75.8, 74.2 and 63.3%, respectively.

안전식품과 공해식품

  • 백광호
    • Food Industry
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    • s.17
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    • pp.73-75
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    • 1973
  • 요즘 쩍하면 식품공해를 내세운다. 이는 현대인이 만들어 놓은 입맛이 싹 가시는 반갑지 않은 용어이다. 어떤 식품을 많이 먹거나 계속 먹으면 몸에 해롭다는 것이 이른바 식품공해인데 글자 그대로 해로울 뿐, 해독은 되지 않는 정도이다.

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