• Journal of Plant Biotechnology
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• v.42 no.3
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• pp.239-244
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• 2015

Calanthe striata f. sieboldii Decne. ex Regel is a terrestrial orchid with beautiful flowers arranged in racemose inflorescences. This species is threatened due to over-collection and loss of suitable habitats. Asymbiotic germination is useful in the conservation efforts to re-establish plants in the wild, and for commercial propagation. In this study, we investigate the effects of sodium hypochlorite (NaOCl), three types of culture media (Phytomax Orchid Maintenance - POM, Seed Germination Maintenance - SGM, and Murashige and Skoog 1962 - MS), and plant growth regulators on embryo swelling, protocorm formation, and embryo diameter of C. striata f. sieboldii. Treatment with 1% NaOCl for 30 min greatly enhanced embryo swelling (28.3%), embryo diameter ($205.8{\mu}m$), and embryo protocorm formation (54.8%) compared to seeds without NaOCl treatment (embryo swelling 8.5%, embryo diameter $14.6{\mu}m$, and protocorm formation 13.4%) on POM medium. Protocorm formation on POM medium supplemented with 1.0 mg/L N6-benzyladenine (BA) (95.6%) was better than the control (54.5%). Additionally, the effects of activated charcoal (AC) and sucrose on seedling growth in in vitro culture were examined. The protocorm converted into healthy plants with well-developed shoot primordia on the POM medium with AC and sucrose. The most suitable conditions for seedling growth after 10 weeks of culture were the POM medium with AC or sucrose. These results show effective asymbiotic seed germination and in vitro seedling growth of C. striata f. sieboldii.

• Korean Journal of Plant Resources
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• v.19 no.2
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• pp.315-322
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• 2006

This experiment was conducted to investigate the induction rate of callus and antioxidative activity of rosemary and lavender. Callus induction rate of rosemary was 92% and 94% at 0.5mg/L and 1.0mg/L of 2,4-D in the media, respectively. The antioxidative activities of rosemary appeared differently according to the callus cultured under various concentrations of plant growth regulators. The strongest effect was observed at 0.1mg/L of 2,4-D extract, with $43{\pm}1.5{\mu}g/mL$ of an RC50 value. Callus induction rate of lavender was 98% and 99% at 1.0mg/L of 2,4-D and 1.0mg/L of NAA in the media, respectively. The regeneration rate of roots lavender was 85% at 1.0mg/L of NAA in the media. Antioxidative activities of lavender appeared diffently according to the callus cultured under plant growth regulators. The strongest effect was observed in 0.1mg/L of 2,4-D extract, with $37.6{\pm}0.9{\mu}g/mL$ of an RC50 value, which could be compared with value of leaf.

• Korean Journal of Plant Tissue Culture
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• v.26 no.3
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• pp.197-203
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• 1999

The albino plants of tobacco (Nicotiana tobacum L. cv. BY-4) were isolated from seed populations that were induced by heavy-ion ($^{14}N$) beam irradiation to proembryo and the in vitro growth and differentiation have been characterized. The in vitro cultured albino plants showed significant reduction of chlorophyll content and possessed larger number of stomata on both upper and lower epidermis than that of wild-type plants. Stem growth of the mutants remained dwarfed, however, the internode recovered its normal length after GA$_3$ treatment (10.0mg/L) on the MS medium containing sucrose under continuous light. When explants of leaf blades of albino plants were cultured, multiple shoots formed directly on MS medium containing 1.0mg/L of BAP or kinetin and a large number of calli were induced on the MS medium containing 1.0mg/L NAA or 1.0 mg/L 2,4-D. The albino calli regenerated multiple albino plantlets in the MS medium containing 0.1mg/L NAA + 1.0 mg/L BAP. No significant differences between the wild-type and albino plants were detected in the multiple shoot induction, callus formation from the explants and the plantlets regeneration from calli. In addition, albino plants have a similar organogenesis Pattern to that of the wild-type in the media with different combinations of NAA (0 to 5.0mg/L) and BAP (0 to 5.0mg/L) treatment. These results indicate that the albino mutant has the same normal regeneration ability as that of wild-type, although the mutant has lost functions in photosynthesis, such as pigmentation.

• Korean Journal of Food Science and Technology
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• v.52 no.3
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• pp.284-289
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• 2020

The cultivars of wine grapes 'Doonuri', 'Narsha', 'Cabernet Sauvignon', and 'Merlot' were cultivated in Modified-T (MT), Modified-T Cane pruning (MTC), and Geneva Double Curtain (GDC) trellises, and the changes in vine growth, fruit and wine characteristics were compared for two years (2018 and 2019). With respect to the vine growth characteristics, the wine grape cultivars cultivated in the GDC trellis were significantly inferior to those cultivated in the MT or MTC trellises. The grape yields of the cultivars cultivated in GDC trellise increased by 1.5 to 2 times cultivated in the GDC trellises compared to those cultivated in the other trellis, however, the fruit characteristics (total soluble solids, titratable acidity, and berry weight) did not differ among the differently cultivated groups. Moreover, the anthocyanin content and red color of the wine were significantly enriched in all red wine grape cultivars cultivated in the GDC trellises.

• Journal of Korean Society of Forest Science
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• v.78 no.2
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• pp.189-197
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• 1989

This study was carried out to produce the grafts of Juglans sinensis by juvenile grafting method which epicotyl of newly germinated seeds were used as stocks and juvenile fresh shoots were used as scion. The results obtained were as follows ; 1. When plastic tube installed covering up seed with soil up to 6cm height for diameter increment of epicotyl, the epicotyl can be grown up to thickness of 10mm. 2. When the soft fruit branches and the soft water sprout with the terminal bud 8cm to 12cm long were used as scions, the survival rates showed 90 Percent. 3. The optimum date for making juvenile grafts was around the 20th of May, and the survival rates of grafted seedlings showed 86 percent in average. 4. The grafted seedlings showed first sprouting the 15th of June, that is 25 days after making graft, and the sprouting rate was 72 percent. 5. The height-growth of grafted seedlings finished at the end of July, and diameter growth lasted into the end of October. 6. There was positive correlation between the height of grafted seedlings and the diameter at root collar. 7. In general, it takes two years to make plantable graft seedlings from hardwood scion and stock, but the juvenile graft seedlings can be easily obtained in a year and so it seems to be economic.

• Korean Journal of Plant Resources
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• v.37 no.4
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• pp.431-438
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• 2024

Hops enhance beer flavor and bitterness, acting as a preservative. In recent years, the booming trend of craft beer has prompted the introduction of foreign hop varieties for cultivation and production in South Korea. This study focuses on developing efficient in vitro culture condition of the hop (Humulus lupulus L.) variety 'Cascade' for treatments of plant growth regulators, i.e. IAA and Cytokinin. Using Auxin IAA and Cytokinin 2iP, Zeatin, BAP, and TDZ on MS medium as plant growth regulators, the experiment involved removing three nodes from the shoot apex. In vitro hop culture showed the highest shoot proliferation rate when only IAA was added, with approximately 21% higher compared to the combination with Cytokinin. Notably, IAA 0.1 mg/L + BAP 1 mg/L resulted in a superior shoot proliferation rate of around 91%. IAA 0.1 mg/L + BAP 1 mg/L was advantageous for shoot elongation. Callus induction occurred with TDZ, while control or IAA-only conditions exhibited shoot and root growth. Cytokinin addition led to callus formation and increased weight. Assessing survival and soil adaptation during in vitro hop seedling acclimatization involved maintaining near 100% humidity initially, gradually reducing it over three weeks. When transferred outdoors, 9 out of 10 seedlings acclimated successfully, confirming a 90% acclimatization rate.

• Horticultural Science & Technology
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• v.33 no.1
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• pp.1-10
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• 2015

This study was conducted in three years (7-9 years after planting) to investigate vegetative growth, yield, fruit quality, and return bloom for optimum crop load based on different planting densities of adult 'Fuji'/M.9 apple trees. As plant materials, 'Fuji'/M.9 apple trees planted at $3.5{\times}1.5m$ (190 trees per 10 a), $3.5{\times}1.2m$ (238 trees per 10 a), and $3.2{\times}1.2m$ (260 trees per 10 a) spacing and trained as slender spindles were used. The crop load was assigned to five different object ranges as follows: 55-64, 65-74, 75-84, 85-94, and 95-104 fruit per tree. TCA increment, total shoot growth, return bloom, yield per tree, and yield efficiency tended to increase as planting density decreased, and fruit weight and soluble solid content tended to increase as the object range of crop load decreased. Fruit red color tended to increase as shoot growth decreased. For apple trees planted with 238 trees and 260 trees per 10a, biennial bearing occurred when the crop load was over 85-94 and 75-84 fruits, respectively. However, biennial bearing did not occur when the crop load was 95-104 fruits in apple trees planted with 190 trees per 10a. Accumulated yield tended to increase as planting density and crop load increased, but that of biennial bearing did not show such a difference. Based on our results, optimum crop load recommendations are to set 95-104 fruits per tree in 'Fuji'/M.9 mature apple trees planted at 190 trees per 10a, 75-84 fruits per tree at 238 trees per 10a, and 65-74 fruits per tree at 260 trees per 10a.

• Journal of Bio-Environment Control
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• v.13 no.1
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• pp.51-55
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• 2004

The most commonly used inorganic nutrient compositions such as Murashige & Skoog medium have been optimized for heterotrophic growth. Therefore, they may not be optimal for photomixotrophic and photoautotrophic growth of plantlets. In photomixotrophic micropropagation, emdium sugar level is often lowered, while light and $CO_2$ levels in vessel are raised, and chlorophyllous explants are used to facilitate photosynthetic carbon acquisition. In a factorial experiment effect of addition (+) and omission(_) of organic materials (OM, 0.5 g ${\cdot}$ $m^{-3}$ each of thiamine, nicotinic acid and pyridoxine and 100 ${\cdot}$ $m^{-3}$ myo-inositiol) combined with three sucrose levels (0, 15, and 30 kg ${\cdot}$ $m^{-3}$) was tested on the growth of potato plantlets. Each of nodal cuttings with a leaf was cultured on 0.1${\times}$$10^{-4}m^{-3}$) MS agar ( 8 kg ${\cdot}$ $m^{-3}$) medium (pH 5.80 before autoclave) in glass test tubes (100 mm${\times}$25mm) capped with a sheet of transparent film with a 6 mm diameter gas permeable filter (5.1 air exchanges ${\cdot}$$h^{-1}$). Cultures were maintained in a room for 27 days at $23^{\circ}C$, 50% RH, 350-450${\mu}mol\;{\codt}\;mol^{-1}CO_2$, 16 h ${\cdot}$ $d^{-1}$ photoperiod at 13${\mu}mol\;{\codt}\;m\;{\codt}\;s^{-1}$ PPFD provided by white cool fluorescent lamps. Growth of potato plantlet in the +OM and -OM treatments were similar, while medium pH was 0.2 scale lower in the latter. Dry weight, % dry matter, and stem diameter enhanced, while shoot to root dry weight ratio, leaf area, chlorophyll concentration per gram dry weight, and medium pH decreased with increasing initial sucrose level. Interaction between OM and sucrose levels was observed in shoot length and medium pH. Results indicate that OM can be omitted from the medium without detrimental effect while addition of sucrose was beneficial for the photomixotrophic growth of potato plantlets under raised light and $CO_2$.

• Weed & Turfgrass Science
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• v.2 no.1
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• pp.23-29
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• 2013

This study was conducted to clarify effects of weed control methods on damages from agricultural chemicals of pear trees, growth of weeds and states of pear trees after treating pear orchards with different methods of weed controlling, density of GLU and application times. The growth and occupation rate were investigated after 10, 20 and 40 days of weeding treatment. According to a result of the first treatment conducted when weeds in the lower parts of crown in a pear orchard began to grow and grew about 20 cm, unwoven cloth covering showed the highest control value with 100% in all 14 kinds of grasses. In comparison, Stellaria aquatica and catchweed bedstraw showed 96.7% and 97.3% respectively in the 20 DAT investigate after the first treatment of GLU 540 g a.i. ha-1 and the high control value of 100% in other all kinds of grasses. According to an investigation of stalk enlargement, length of new shoot and the number of new shoot made to know differences in tree growth following treatment of the lower part of crown, to use weed killers two or three times a year or to eliminate grasses with machines have positive effects on cross growth of pear trees.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.6
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• pp.380-386
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• 2001

This study was conducted to investigate the influence of the root zone temperature on the changes in soil physicochemical environment and the growth of the apple trees. Root zone temperatures applied were 20. 25. 30. and $35^{\circ}C$. A pot experiment of 4-year-old fruit-bearing Fuji/M.26 apple trees was done from May 15. 1997 to August 15. 1997. Shoot length and shoot number of 4-year-old Fuji/M.26 was longest at $30^{\circ}C$ but decreased at $35^{\circ}C$. Oxygen concentrations in soil air were maintained from 17 to 18% at $20^{\circ}C$ but fluctuated from 10 to 14% at the end at $35^{\circ}C$. Carbon dioxide concentrations in soil air was highest at $35^{\circ}C$. Organic matter contents in soil decreased as root zone temperature and duration of treatment increased. Nitrate and ammonium contents in soil increased with rising root zone temperature in the beginning but nitrate contents decreased at $35^{\circ}C$ as time progressed. Nitrate content was higher in 10~15cm subsoil than in 25~30cm subsoil.