• Horticultural Science & Technology
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• v.35 no.4
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• pp.490-498
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• 2017

The in vitro growth of virus-free sweet potato [Ipomoea batatas (L.) Lam.] plantlets was investigated under different light sources: fluorescent lamp (control); red (660 nm), blue (460 nm), white light-emitting diodes (LED), and two mixtures of blue and red LED (R:B = 8:2, and 7:3). Single node explants (10 mm) of three cultivars ('Matnami', 'Shincheonmi', and 'Yeonhwangmi') were cultured on Murashige and Skoog medium supplemented with $0.2mg{\cdot}L^{-1}$ 6-benzyladenine for 4 weeks. Explants were exposed to $150{\pm}5{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ photosynthetic photon flux at a distance of 20 cm, constant temperature of $25^{\circ}C$, and under 16/8-h (day/night) photoperiod. Using the same method, the in vitro growth of 10 cultivars under red LED was also compared. After 3 weeks, vine length was highest in plantlets cultured under red LED, and lowest in plantlets cultured under blue LED. Fresh and dry weights were also greatest in plantlets cultured under red LED. Compared to the control, vine thickness was significantly higher in plantlets grown under white LED and the 7:3 R:B LED mixture. Significant differences were observed among the 10 cultivars grown under red LED. 'Matnami', 'Shincheonmi', and 'Shinhwangmi' all had excellent vine lengths, and fresh and dry weights. Compared to the control, vine elongation of sweet potato plantlets was most effective under red LED, and culture duration was about 1 week shorter.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.552-560
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• 2010

$GA_{4+7}$+BA, a plant growth regulator for induction of feathering in young apple tree and increasing fruit size, was applied by various methods on 'Gala'/M.9 apple trees in high density orchard for 4 years to investigate its effect on fruit and shoot growth. $GA_{4+7}$+BA($80-300mg{\cdot}L^{-1}$) increased fruit length, fruit weight, and L/D ratio regardless of application methods, but it did not affect soluble solid content, acidity, leaf area, and chlorophyll. Seed number was not affected by $GA_{4+7}$+BA application, however, more immature seeds was observed in treated 'Gala' fruit. Shoot growth was increased when spraying $GA_{4+7}$+BA at tree crown but not affected when spraying at fruit directly. More significant fruit growth was observed when $GA_{4+7}$+BA was applied on the fruits between late of May and early of June when fruit cell division ended; however, high concentration of $GA_{4+7}$+BA resulted in lower fruit storability because of lower firmness. Hence, more attention should be paid when applying high concentration of $GA_{4+7}$+BA to small sized fruit cultivars like 'Gala'.

• Journal of Bio-Environment Control
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• v.15 no.3
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• pp.277-282
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• 2006

For searching the natural herbicide-components, the seed germination and seedling growth of receptor plant species (Brassica campestris, Sesamum indicum, Perilla frutescens and Echinochloa crus-galli) were investigated in four solvent-extracts extracted from Coptis chinensis Franch. The seed germination of receptor plant species was largely inhibited in 2,000 ppm of ethyl acetate compared to the control, and it was inhibited in order of P. frutescens, B. campestris, E. crux-galli, and S. indicum. In seedling growth, the shoot and root elongations of receptor plant species were inhibited in order of S. indicum, P. frutescens, B. campostris, and E. crus-galli. Root elongation was remarkably reduced in order of $H_2O$, butyl alcohol, and hexane, ethyl acetate extracts. Of four receptor plant species, seed germination and seedling growth of B. campestris and S. indicum showed the species-specific reaction to the solvent-extracts extracted from C. chinemis. $H_2O$ extract had a natural herbicide potential to the seed germination or root elongation in B. campestris and S. indicum. The result can be provided a basic data f3r the development of natural herbicide.

• Korean Journal of Plant Resources
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• v.24 no.2
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• pp.168-173
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• 2011

This study was carried out to optimize the embryogenic callus induction and plant regeneration from mature seeds of Sorghum bicolor. The effect of growth regulators was investigated on formation of embryogenic callus. The highest frequency of embryogenic callus was observed when the mature seeds were cultured on B5 medium supplemented with 2 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D). The highest frequency of plant regeneration from embryogenic callus was observed on MS medium with 0.5 $mg\;l^{-1}$ 6-benzyl amino purine (BAP) and 0.25 $mg\;l^{-1}$ indole-3-butyric acid (IBA) to optimize the shoot regeneration. High concentration of BAP (1 $mg\;l^{-1}$) supplemented with IBA (0.25 $mg\;l^{-1}$) was effective combination for shoot multiplication. MS medium supplemented with 1 $mg\;l^{-1}$ IBA was found to be the most effective for inducing roots. Normal rooted plantlets were transferred to the greenhouse for hardening with over 90% survival rate. Hence, this reproducible protocol could be useful for mass propagation and genetic transformation of S. bicolor.

• Journal of Korean Society of Forest Science
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• v.104 no.1
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• pp.76-83
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• 2015

Old growth and protected-trees are worth history and culturally in Korea. This research carried out to investigate habitat characterization and the vegetative propagation methods of Salix chaenomeloides(100-600 years old trees) in Gyeongsangnamdo Province. It has been preserved in good and grow naturally in area surrounding village(4 trees), riverside(1 tree) and field(1 tree). Cutting from old growth and protected trees was conducted using 10 kinds of bedsoils. It was survived above 93% in all bedsoils. Growth of cuttings is a distinct difference according to bedsoils and tree ages. Growth of cutting was high on CBS, followed mixed bedsoils(VPMP) including vermiculite, peatmoss and perlite. Root collar diameter was difference depend on tree ages and bedsoils. All trees are produced 1 to 4 branch. Root growth of cutting was 20 cm on CBS and VPMP, which is the best shoot growing on cutting. Bedsoil with physico-chemical characteristics of high moisturizing ability, high air permeability, high EC value and slightly acid may be suitable for efficiently cutting propagation of old growth and protected S. chaenomeloides. CBS and VPMP bedsoil are favorable for propagating this species by cuttings.

• Korean Journal of Organic Agriculture
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• v.23 no.2
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• pp.267-280
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• 2015

The study was conducted to evaluate effects of time and degree of defoliation on growth of 'Wonhwang' pear (Pyrus pyrifolia Nakai) trees managing with low pesticides as well as regrowth of cuttings in vitro. Treatments included degree of defoliation (20% and 60%) with time of defoliation (Early-Aug, End-Aug, and Early-Sep); Early-Aug (20%), Early-Aug (60%), End-Aug (20%), End-Aug (60%), Early-Sep (20%), Early-Sep (60%), and No defoliation. No defoliation and Early-Sep (20%) defoliation increased growth of water sprouts and new shoots, which were improved by delayed defoliation or 20% of defoliation. Total-C, total-N, B, and free sugar contents increased in No defoliation-shoots but decreased in End-Aug (60%)-shoots. Delayed defoliation increased total-C, total-N, and free sugar in shoots, with high contents of C, K, Ca, Mg, and B observed for 20% of defoliation-trees. Fruit yield and weight or fruit length increased in No defoliation, End-Aug (20%) defoliation, and Early-Sep (20%) defoliation, but reduced in End-Aug (60%). Fruit soluble solids content reduced in defoliation in August. Time of defoliation did not affect the fruit yield and fruit quality, while degree of defoliation influenced yield and fruit weight and length. Defoliation at End-Aug (60%) mostly increased the leakage rates of the stem cuttings at $-18^{\circ}C$ and $-21^{\circ}C$ in vitro and reduced the germination rates at $-24^{\circ}C$ and $-27^{\circ}C$. Under comparison of time and degree of defoliation, the Early-Sep defoliation increased germination rates of the stem cuttings at $-27^{\circ}C$ in vitro, and 60% of defoliation decreased the germination rates compared to the 20% of defoliation.

• Korean Journal Plant Pathology
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• v.11 no.2
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• pp.132-138
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• 1995

우리나라 사과원에서의 토양병 발생현황과 발생실태를 1991년부터 1993년까지 3년간 조사한 결과, 전체 조사주수의 약 8.1%가 각종의 토양 병원균에 감염되어 있는 것으로 나타났다. 토양병 중 자주날개 무늬병의 발생빈도가 5.1%로 가장 높게 조사되었으며, 흰날개무늬병과 역병의 이병주율은 각각 2.5%와 0.2%로 조사되었다. 자주날개무늬병에 감염된 나무에서는 잎의 황화 및 조기낙엽, 신초생장 억제, 과실 생육 불량 등의 지상부 병징이 관찰되었고, 지하부 뿌리 표면에는 적자색 실모양의 균사나 균사속이 관찰되었다. 감염된 뿌리의 표피는 쉽게 벗겨지고 목질부로부터 잘 이탈되는 특징이 있었다. 흰날개무늬병균에 감염된 나무의 뿌리는 흰색의 균사 혹은 균사막으로 싸여 있으며 이 균사막은 시간이 경과하면 회색 내지 흑색으로 착색되었다. 굵은 뿌리의 표피를 제거하면 목질부에 백색 부채모양의 균사막과 실모양의 균사속을 확인할 수 있었다. 이 병원균은 목질부까지 부패시키므로 병의 증세가 심하게 나타났다. 자주날개무늬병은 새로 개원한 10년 이하의 과원에서 발생이 많았다. 자주날개무늬병은 왜화도가 높은 대목에서 발병율이 높았으나 흰날개무늬병은 대목의 종류에 관계없이 발명되었다. 자주날개무늬병은 식양토 과원에서 발생이 많았으며 흰날개무늬병은 사양토 과원에서 발생이 많았다.

• Journal of Bio-Environment Control
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• v.22 no.4
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• pp.366-370
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• 2013

The effects of the early tree shapes with renewal by top-working on Y-shaped peach tree, and the influence on the maintaining fruit quantity with gradual renewal of interstock cultivar were determined. In the comparison of the places of top-working tree, top working tree on the inside of the main branch of interstock cultivar had higher graft union rate and branch growth than those of top working tree on the outside. Tree width, basal diameter of shoot, and number of bearing shoots were smaller in top working tree than in replanted tree. Although labor time was not different to control top working tree and replanted tree, labor time was much required to manage top working tree with interstock cultivar. Accumulated fruit production was 2,384 kg/10a in top working tree and 2,025 kg/10a in replanted tree for three years. However, top working tree had no loss of fruits because interstock cultivar of top-working tree had 3,727 kg/10a of fruits. No variation on fruit quality was observed between top working tree and replanting tree. In terms of economic value of top-working tree, labor to manage interstock cultivar, fertilizer price, fruit bagging, and grading and packing price increased. However, fruit production increased, and price of seedling, rental equipment, pulling-out trees, and repairing supporting system decreased. Therefore, gradual renewal of topworking tree has effects on the maintaining fruit quantity, supplementation on fruit loss, and renewal cultivar.

• Korean Journal of Plant Resources
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• v.29 no.5
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• pp.598-603
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• 2016

The aim of this study was to elucidate the influence of vigor of a bearing shoot in ‘Bluecrop’ Highbush Blueberry (Vaccinium corymbosum L.) on growth characteristics of shoots and fruits. Bearing shoots were classified with BS (bearing shoot) and BMB (bearing mother branch). The vigor of bearing shoots were divided into four arbitrary categories; A was thin (< 6.0 ㎜) BMB and short (< 10 ㎝) BS, B was thin BMB and long (≥ 10 ㎝) BS, C was thick (≥ 6 ㎜) BMB and short BS and D was thick BMB and long BS. Shoots from D were longer (6.5 ㎝) and thicker (1.70 ㎜) than those from the others. Shoots of D had more leaves (5.8 ea) than those of the others. Leaf area of D was larger (13.5 ㎠) than those of the others. The first harvest of D was one week faster than the others. Ratio of big berry (>14 ㎜) from the long BSs was higher (B : 41.7, D : 46.8%) than that from the short BSs. Soluble solid content of small berrys did not show any different according to vigor of bearing shoots, but soluble solid content of big berrys of the long BSs was higher (B : 16.2, D : 15.6°Bx) than those of the short BSs. The thickness of BMB did not affect ratio of fruit size and soluble soild content. The long BSs would be proper than the short BSs for bearing bigger fruits.

• Horticultural Science & Technology
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• v.19 no.3
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• pp.367-372
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• 2001

'Kyoho' grape (Vitis labruscana L.) has currently cropped twice a year in plastic greenhouses. However, there are problems with low fruit quality in the second cropping owing to low temperatures and short photoperiods. This experiment was conducted to investigate the effect of root zone heating and $CO_2$ enrichment in plastic greenhouse on the vine growth and fruit quality of 'Kyoho' grape in double cropping system. The internode length of shoots, leaf area and leaf dry weight at the treatment of soil heating near root zone was significantly different regardless of $CO_2$ enrichment. There were no significant differences in fruit bunch and berry weight, titratable acidity, coloration degree and berry shattering among the treatments, but the soluble solids significantly increased by root zone heating. Photosynthetic rate increased with increasing $CO_2$ concentration from 300 to $800{\mu}mol{\cdot}mol^{-1}$ in sunny day, whereas it didn't increase in cloudy day regardless of $CO_2$ enrichment.