• Korean Journal of Food Science and Technology
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• v.31 no.2
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• pp.535-539
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• 1999

Soybean has drawn much attention mainly due to its chemopreventive action as well as antiestrogenic effect. Although suppression of breast and prostate cancers were believed to be exerted via antiestrogenic or antiandrogenic activity of genistein, its mechanism of prevention against other cancers has not been clearly demonstrated. We proposed that prevention by soybean from other cancers than sex hormone -related cancers was achieved via modulation of drug-metabolizing enzymes. Addition of acid hydrolysate of 80% methanol extract of soyflour to diet caused a significant induction of quinone reductase, an anticarcinogenic marker enzyme and one of drug-metabolizing enzymes, in mouse lung while it suppressed arylhydrocarbon hydroxylase, involved in bioactivation of procarcinogens, in kidney and small intestine. It is likely that active components exist in a conjugated form and released by acid hydrolysis to be able to affect drug-metabolizing enzyme and exert chemopreventive activity. Benzo(a)pyrene-induced tumor development in mouse lung was greatly reduced by soybean extract supplementation, which is consistent with the extract's capability to modulate favorably arylhydrocarbon hydroxylase and quinone reductase towards chemoprevention.

• Korean Journal of Plant Tissue Culture
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• v.24 no.5
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• pp.263-268
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• 1997

Effects of plant growth regulators and condition on shoot induction from internode explants of Actinidia arguta were investigated. Optimal condition for shoot induction was obtained when internode explants were cultured on MS medium containing 3.0 mg/L zeatin. The frequency showed about 62.5% under darks condition at 27$^{\circ}C$ after 6 weeks of culture. Regenerated shoots were rooted on WPM medium supplemented with 0.5 mg/L NAA and the regenerants were acclimated to an artificial soil with mixture (perlite : vermiculite = 1:1, v/v) for further growth and successfully transplanted to the soil in a highly humidified greenhouse. Histological observations revealed that meristemoids were formed from small and isodiametric cells, and then developed to shoot primordia.

• Journal of the Korean Institute of Gas
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• v.17 no.4
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• pp.9-17
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• 2013

Butanethiol is known as a typical odorant with hydrogen sulfide, methyl mercaptan, methyl sulfide, but on the physical and chemical properties and biological hazard assessment, including inhalation toxicity data are very scarce. Butanethiol as a colorless transparent liquid, and has physic-chemical characteristics with flash point as $-23^{\circ}C$ and strong fire risk, boiling point $84-85^{\circ}C$, vapor pressure 80.71 mmHg ($25^{\circ}C$), freezing point $-140.14^{\circ}C$. From whole body exposure with SD rats, the $LC_{50}$ is above 2,500 ppm (9.22mg/L), and then it is classified as the acute toxic chemical (inhalation) category 4 according to the governmental notification No. 2012-14.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.10
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• pp.1295-1301
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• 2009

This study was carried out to investigate the mutagenic, antimutagenic, cytotoxicity and antitumor effect of Codonopsis lanceolata (CL). CL was extracted with 70% ethanol and then further fractionated to hexane, chloroform, ethyl acetate, butanol and water. Antimutagenic, cytotoxicity and antitumor effects of CL extracts were measured by using Ames test, SRB method, and the tumor growth inhibition test. CL extracts did not show any mutagenicity in the Ames test; however, 70% ethanol extracts and its fractions had strong antimutagenic effects against mutation induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and 4-nitroquinoline-1-oxide (4NQO). The ethyl acetate fraction of CL (200 ${\mu}g$/plate) showed approximately 72.1% inhibitory effect on the mutagenesis induced by 4NQO against TA98 strain, whereas 69.6% and 67.0% inhibitions were observed on the mutagenesis induced by MNNG and 4NQO against TA100 strain. In anticancer effects, the cytotoxicity of CL extract and its fractions against cancer cell lines including human cervical adenocarcinoma (HeLa), human hepatocellular carcinoma (HepG2), human breast adenocarcinoma (MCF-7), human lung carcinoma (A549) and transformed primary human embryo kidney (293) were investigated. The treatment of 1 mg/mL CL ethyl acetate fraction had the highest cytotoxicity of 74.5%, 70.7% and 80.3% against HeLa, MCF-7 and A549 cells, respectively. In contrast, the extract and its fractions showed only 2$\sim$31% cytotoxicity for a normal human kidney cell line (293). In vivo anticancer effect of CL extract was tested using Balb/c mice transplanted sarcoma-180 cells. CL ethyl acetate fraction showed the highest inhibition rate of 56.4% at the 50 mg/kg concentration.

• Clinical and Experimental Pediatrics
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• v.46 no.12
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• pp.1186-1193
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• 2003

Purpose : Fragile sites are points on chromosomes which tend to break non-randomly when exposed to specific chemical agents or conditions of tissue culture. The chromosomal break induced by the antineoplastic drug, 1-${\beta}$-D-arabinofuranosyl-cytosine(Ara-c), was investigated to study the laboratory conditions in which the incidence of chromosomal break could be enhanced. Besides, the fragile sites induced by Ara-C were investigated and compared to the already known locations of the specific chromosomal alterations observed in specific neoplasms. Methods : T-lymphocytes from theree normal males and three females were cultured for 48 hours. Cells from each individual were exposed to the Ara-C for an additional 24 hours. After the caffeine was added during the last six hours culture, the metaphase chromosomes were prepared following the conventional method. A site was considered fragile if it was found to break two or more per 100 chromosomal breaks in more than four of six individuals tested. Results : Ara-C induced 252.1 chromosomal breaks per 100 mitotic cells and this result was significantly higher than that of the control, which induced 25.2 breaks(P<0.05). The incidence of the chromosomal break by Ara-C was higher, if cultured in the MEM-FA, which has no folic acid, than in the RPMI 1640 which contains enough folic acid(P<0.05). The most common break site by Ara-C was 3p14.2(FRA3B). There were 20 fragile sites induced by Ara-C. Among these 20 fragile sites, seven coincided with the locations of the mapped oncogenes, JUN, SKI, REL, N-MYC, FHIT, MET, ETS-1, and FOS. Conclusion : S phase specific chemotherapeutic agent, Ara-C, induced the expression of the chromosomal fragile sites effectively using the T-lymphocyte in vitro. Some of the fragile sites by Ara-C highly coincided with the oncogenes and neoplasm specific chromosome breakpoints. In this regard, the fragile sites reported here could provide the unknown neoplasm related chromosomal alternation points.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.1
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• pp.25-31
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• 2009

This study was carried out to investigate the mutagenic, antimutagenic, cytotoxicity and antitumor effects of Adenophora triphylla (AT). AT was extracted with 70% ethanol and then further fractionated to hexane, chloroform, ethyl acetate, butanol and water. Antimutagenic, cytotoxicity and antitumor effects of AT extracts were measured by using Ames test, SRB method, and the tumor growth inhibition test. AT extracts did not show any mutagenicity in the Ames test; however, 70% ethanol extracts and its fractions had strong antimutagenic effects against mutation induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and 4-nitroquinoline-1-oxide (4NQO). The ethyl acetate fraction of AT (200 ${\mu}g$/plate) showed approximately 66.5% inhibitory effect on the mutagenesis induced by 4NQO against TA98 strain, whereas 83.3% and 75.1% inhibitions were observed on the mutagenesis induced by MNNG and 4NQO against TA100 strain. In anticancer effects, the cytotoxicity of AT extract and its fractions against cancer cell lines including human cervical adenocarcinoma (HeLa), human hepatocellular carcinoma (Hep3B), human breast adenocarcinoma (MCF-7), human gastric carcinoma (AGS), human lung carcinoma (A549) and transformed primary human embryo kidney (293) were investigated. The treatment of 1 mg/mL AT ethyl acetate faction had the highest cytotoxicity of 79.9%, 74.9%, 66.0%, 71.0% and 74.3% against HeLa, Hep3B, MCF-7, AGS and A549 cells, respectively. In contrast, the extract and its fractions showed only $3{\sim}36%$ cytotoxicity for a normal human kidney cell line (293). In vivo anti-cancer effect of Adenophora triphylla extract was tested using Balb/c mice transplanted sarcoma-180 cells. Adenophora triphylla ethyl acetate fraction showed the highest inhibition rate of 37.2% at the 50 mg/kg concentration.

• The Journal of the Korean bone and joint tumor society
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• v.13 no.1
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• pp.48-54
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• 2007

Purpose: To evaluate clinical results of the tumor prosthetic replacement for pathologic fracture of the proximal femur with extensive bony destruction due to metastatic bone tumors. Materials and Methods: From 2005 October to 2006 October, resection of proximal femur and tumor prosthesis replacement was performed for metastatic bone tumors extensively involving trochanteric area in 6 patients. The mean age of overall patients was 61(range, 48~77). Mean clinical follow up was 10.5 months (range, 6~16). Primary tumor consisted with multiple myeloma in 2 patients, lung cancer in 1, breast cancer in 1, transitional cell carcinoma of the renal pelvis in 1, unknown origin in 1 patient. $MUTARS^{(R)}$ proximal femur (Implantcast, Munster, Germany) were used in all cases. The functional evaluation in the lower extremities was performed with Musculoskeletal Tumor Society 1993 scores. The degree of pain relief after surgical treatment was assessed with Visual Analogue Scale (VAS). Results: At the final follow up, all of the patients survived. The mean lower extremities functional score of Musculoskeletal Tumor Society 1993 was 17.8(59.3%)(range, 12~25). VAS was improved to 2.5 from 8.5 after the surgical treatment. Ambulation was encouraged as soon as possible and mean duration after surgery for ambulation was 7.3(range, 3~16) days. Post-operatively, there was no surgical infection, periprosthetic fracture or loosening. Dislocation occurred in one out of six cases. Conclusion: Resection of proximal femur and tumor prosthesis replacement for metastatic bone tumors around proximal femur extensively involving trochanteric area seems to be safe procedure in view of post-operative complication, and effective for functional restoration as well as pain relief. Thus, it is accordant to the treatment principle of the metastatic bone tumors.

• The Korean Journal of Petroleum Geology
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• v.2 no.2 s.3
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• pp.59-70
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• 1994

The Cenozoic geological structures and the tectonic evolution of the southern Ulleung Basin were studied with seismic profiles and exploration well data. Basement structure of the Korea Strait is distinctly characterized by normal faults trending northeast to southwest. The normal faults of the basement are most likely related to the initial liking and extensional tectonics of Ulleung Basin. Tsushima fault along the west coast of Tsushima islands runs northeastward to the central Ulleung Basin. The Middle Miocene and older sequences in the Tsushima Strait show folds and faults mostly trending northeast to southwest. These folds and faults may be interpreted as a result of compressional tectonics. The Late Miocene to Qauternary sequences are not much deformed, but numerous faults mostly N-S trending are dominated in the Tsushima Strait. The Ulleung Basin was in intial rifting during Oligocene, and then active extension and subsidence from Early to early Middle Miocene. Therefore SW Japan separated from Korea Peninsula and drifted toward southeast, and Ulleung Basin was formed as a pull-apart basin under dextral transtensional tectonic regime. During rifting and extensional stage, Tsushima fault as a main tectonic line separating SW Japan block from the Korean Peninsula acted as a normal faulting with right-lateral strike-slip motion as SW Japan drifted southeastward. During middle Middle Miocene to early Late Miocene, the opening of Ulleung basin stopped and uplifted due to compressional tectonics. The southwest Japan block converging on the Korean Peninsula caused compressional stress to the southern margin of Ulleung Basin, resulting in strong deformation under sinistral transpressional tectonic regime. Tsushima fault acted as thrust fault with left-lateral strike-slip motion. From middle Late Miocene to Quaternary, the southern margin of Ulleung Basin has been controlled by compressional motion. Thus the Tsushima fault still appears to be an active thrust fault by compressional tectonic regime.

• The Journal of the Petrological Society of Korea
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• v.2 no.2
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• pp.74-94
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• 1993

Metabasites and metapelites in the Mungyong area were intruded by Cretaceous granites with radius of 4-8 km. As the distance from granite body increases, the mineral assemblage of metabasite changes from amphibole + plagioclase through amphibole + plagioclase + epidote to amphibole + plagioclase + epidote + chlorite. The compositional variations of amphibole and plagioclase according to the change of metamorphic grade and bulk rock compositions are very complex. Towards the Mungyong Cretaceous granite body, the mineral assemblage of metapelite changes from chlorite+ muscovite(ch1orite zone) through biotite + chlorite + muscovite(biotite zone) to andalusite+biotite + muscovite${\pm}$chlorite or cordierite+ biotite+ muscovite${\pm}$chlorite(cordierite zone). The estimated metamorphic conditions of cordierite zone are 480~$580^{\circ}C$ 1.5-3.3 kb. The theoretical study on the thermal metamorphism caused by the Cretaceous granite with radius longer than 4 km in the Mungyong area suggests the followings: The degree of metamorphism is mainly determined not by the size of granite body but by the temperature of granite intrusion; The country rocks within 2 km from Cretaceous granite have undergone metamorphism with temperature higher than $500^{\circ}C$, which is consistent with the petrological study in the Mungyong area. Mungyong Cretaceous granite caused a low P/T thermal metamorphism to the country rocks; the amphibolite facies metamorphism to the country rocks within 1-2 km from the granite body and the epidote-amphibolite and greenschist facies metamorphism to the country rocks within 2-5 km.

• The Journal of the Petrological Society of Korea
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• v.6 no.3
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• pp.151-165
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• 1997

The Kyemyeongsan area of Chungju in the NE part of the Ogcheon metamorphic zone, Koera, consists mainly of the Ogcheon Supergroup(Taehyangsan Quartzite, Hyangsanri Dolomite and Kyemyeogsan Formation) and the MeSozoic Chungju granite. The Kyemyeongsan Formation is composed mainly of metamorphic rocks of various grades derived from conglomeratic, basic, acidic, pelitic and psammitic rocks. The basic and acidic rocks show alternated or interfingered appearence, indicating that they were derived form bimodal type of magmatism in rift environment. Conglomeratic rocks overlie acidic volcanic rocks in geneal, but are underlain by both acidic plutonic and volcanic rocks. This indicaties that the acidic magmatism before the formation of conglomeratic rocks was different from that during or after the formation of conglomeratic rocks in its occurrence mode. The geological structure of the Ogcheon metamorphic zone in the Kyemyeongsan area, Chungju was formed at least by three phases of deormation. The first phase deformation(D1) formed a regional-scale sheath-type fold(F1) closed into the east. Its axial phane(S1) strikes NNW to NW and dips WSW to SW. The stetching lineation(L1), related to the sheath-type fold, plunges westward. The second phase deformation (D2) formed asymmetric fold(F2) of ESE-to SE-vergence with NNE to NE striking axial plane(S2) and $20~45^{\circ}/210~230^{\circ}$ plunging axis(L2). The F2 fold reoriented the original westward plunging L1 into northwestward plunging L1 in its lower limb(overturned limb). The third phase of deformation(D3) was recognized as chevron-type fold(F3) with $45^{\circ}/265$^{\circ}$ plunging axis. The F3 fold was formed by the compression of N-S direction, resulting in the reorientation of the original $20-45^{\circ}/210~230^{\circ}$ plunging L2 into mainly $35~45^{\circ}/260~280^{\circ}$ and subsidiarily $30~45^{\circ}/135~165^{\circ}$ plunging L2. After this deformation, open fold with NS striking and steeply E or W dipping axial plane is formed by the compression of E-W direction.