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http://dx.doi.org/10.3746/jkfn.2009.38.1.025

Antimutagenic and Antitumor Effects of Adenophora triphylla Extracts  

Ham, Young-An (Dept. of Food Science and Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University)
Choi, Hyun-Jin (Dept. of Food Science and Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University)
Kim, Soo-Hyun (Dept. of Food Science and Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University)
Chung, Mi-Ja (The Nutraceutical Bio Brain Korea 21 Project Group, Kangwon National University)
Ham, Seung-Shi (Dept. of Food Science and Biotechnology, Division of Biotechnology, School of Bioscience and Biotechnology, Kangwon National University)
Publication Information
Journal of the Korean Society of Food Science and Nutrition / v.38, no.1, 2009 , pp. 25-31 More about this Journal
Abstract
This study was carried out to investigate the mutagenic, antimutagenic, cytotoxicity and antitumor effects of Adenophora triphylla (AT). AT was extracted with 70% ethanol and then further fractionated to hexane, chloroform, ethyl acetate, butanol and water. Antimutagenic, cytotoxicity and antitumor effects of AT extracts were measured by using Ames test, SRB method, and the tumor growth inhibition test. AT extracts did not show any mutagenicity in the Ames test; however, 70% ethanol extracts and its fractions had strong antimutagenic effects against mutation induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) and 4-nitroquinoline-1-oxide (4NQO). The ethyl acetate fraction of AT (200 ${\mu}g$/plate) showed approximately 66.5% inhibitory effect on the mutagenesis induced by 4NQO against TA98 strain, whereas 83.3% and 75.1% inhibitions were observed on the mutagenesis induced by MNNG and 4NQO against TA100 strain. In anticancer effects, the cytotoxicity of AT extract and its fractions against cancer cell lines including human cervical adenocarcinoma (HeLa), human hepatocellular carcinoma (Hep3B), human breast adenocarcinoma (MCF-7), human gastric carcinoma (AGS), human lung carcinoma (A549) and transformed primary human embryo kidney (293) were investigated. The treatment of 1 mg/mL AT ethyl acetate faction had the highest cytotoxicity of 79.9%, 74.9%, 66.0%, 71.0% and 74.3% against HeLa, Hep3B, MCF-7, AGS and A549 cells, respectively. In contrast, the extract and its fractions showed only $3{\sim}36%$ cytotoxicity for a normal human kidney cell line (293). In vivo anti-cancer effect of Adenophora triphylla extract was tested using Balb/c mice transplanted sarcoma-180 cells. Adenophora triphylla ethyl acetate fraction showed the highest inhibition rate of 37.2% at the 50 mg/kg concentration.
Keywords
Adenophora triphylla; Ames test; antimutagenicity; antitumor; cytotoxicity;
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Times Cited By KSCI : 8  (Citation Analysis)
Times Cited By SCOPUS : 2
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