• Journal of Acupuncture Research
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• 제22권5호
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• pp.67-77
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• 2005

Objectives : The purpose of this study is to examine if Bee Venom Acupuncture may be effective to the neuropathic pain(mechanical allodynia, cold allodynia) in a rat model of neuropathic pain. Methods : To produce the model of neuropathic pain, under isoflurane 2.5% anesthesia, tibial nerve and sural nerve was resected. After the neuropathic surgery, the author examined if the animals exhibited the behavioral signs of alloynia. The allodynia was assessed by stimulating the medial malleolus with von Frey filament and acetone. Three weeks after the neuropathic surgery, Bee Venom Acupuncture was injected at Hwando(GB30) one time a day for one week. After that, the author examined the withdrawl response of neuropathic rats' legs by yon Frey filament and acetone stimulation. And also the author examined c-Fos in the midbrain central gray of neuropathic rats and the change of WBC count in the blood of neuropathic rats. Results : The Bee Venom Acupuncture injected Hwando(GB30) decreased the withdrawl response of mechanical allodynia in BV-2, BV-3 group as compared with control group. The Bee Venom Acupuncture injected Hwando(GB30) decreased the withdrawl response of chemical allodynia(cold allodynia) in BV-2, BV-3 group as compared with control group. The Bee Venom Acupuncture injected Hwando(GB30) showed the significant difference between control group and BV-2 group, control group and BV-3 group in the c-Fos expression and U count. Conclusion : We have noticed that Bee Venom Acupuncture at Hwando(GB30) decreased mechanical allodynia and cold allodynia in the model of neuropathic pain compared with the control group. C-Fos expression in the central gray of that group was also decreased compared with the control group. Psin control using Bee Venom Acupuncture was accumulated as time goes by. This study can be used as a basic resource on a study and a treatment of pain.

• 동의생리병리학회지
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• 제26권4호
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• pp.462-468
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• 2012

The present study was conducted with the object of examining how ST36 and LR3 electro acupuncture affects the injured side and the intact side in rats with sciatic nerve injury. For this study, we divided rats into a control group that were injured but not treated, experimental group I that were injured and had electro acupuncture on the intact side, experimental group II that were injured and had electro-acupuncture on the injured side, and experimental group III that were injured and had electro-acupuncture on both the intact side and the injured side, and performed behavioral and immunohistochemical tests. The results of this study are as follows. In the results of hot plate test, on Day 1 of experiment, experimental group I did not show a statistically significant difference but experimental group II and III showed a statistically significant difference, and on Day 2 and 3, all of experimental group I, II and III showed a statistically significant difference. In the results of SFI test, on Day 7 of experiment, experimental group I did not show a statistically significant difference but experimental group II and III showed a statistically significant difference, and on Day 14 and 21, all of experimental group I, II and III showed a statistically significant difference. In the results of immunohistochemical test, the expression of c-fos decreased gradually on Day 1, 2 and 3 in all the experimental groups, and the decrease was larger in the experimental groups that had electro-acupuncture than in the control group. In the results of immunohistochemical test, the expression of BDNF increased gradually on Day 7, 14 and 21 in all the experimental groups, and the expression was higher in the experimental groups than in the control group. Summing up the results, ST36 and LR3 electro acupuncture was effective when it was applied to the injured side and to both sides after sciatic nerve injury, but it was also effective in pain relief and nerve regeneration when it was applied to the intact side.

• 한국의학물리학회지:의학물리
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• 제17권2호
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• pp.96-104
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• 2006

본 연구자를 위시한 많은 연구자에 의해 칼슘이 N형 칼슘통로의 비활성화를 촉진시킨다는 것이 보고되었다. 그러나 칼슘에 의한 비활성화 촉진 효과가 고전적인 칼슘의존성 기전에 의해 기인하는지는 아직 확실하지 않다. L형 칼슘통로의 칼슘의존성 비활성화기전을 밝히기 위하여 지금까지 사용해온 방법의 하나는 세포내, 외의 칼슘농도를 변화시켜보는 것이다. 그러므로 본 연구에서는 칼슘의존성 비활성화기전의 존재 여부를 알아보기 위하여 2가 양이온을 1가 양이온인 메틸아민($MA^+$)으로 치환하였다. 선행 연구를 통해 우리는 5초 동안의 긴 저분극 자극 시 바륨과 칼슘을 사용하여 얻은 전류에서 모두 빠른 성분(${\tau}{\sim}150ms$)과 느린 성분(${\tau}{\sim}2,500ms$)의 비활성화가 있음을 알 수 있었다. 본 연구에서 세포외 2가 양이온의 농도가 0이 되도록 하였을 때 빠른 비활성화가 소실된 반면 느린 비활성화에는 영향이 거의 없었다. 또한 바륨를 사용하였을 때보다 10 mV씩 과분극시킨 전압에서의 메틸암모늄 전류 데이터를 비교하여 보았을 때 느린 비활성화의 시정수가 서로 잘 일치하였으며 이 시정수는 막전압이 저분극될수록 감소하는 막전압의존성 비활성화의 특성을 보였다. 본 연구결과와 선행연구의 결과를 종합하여 볼 때 세포외 2가 양이온의 존재는 N형 칼슘통로의 빠른 비활성화가 일어나기 위하여 필수적인 조건이며 이러한 2가 양이온의존성 비활성화기전은 기존의 칼슘의존성 또는 막전압의존성 기전과 다르다는 가설을 제안한다.

• 한국식품영양과학회지
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• 제36권6호
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• pp.670-677
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• 2007

산화적인 스트레스(oxidative stress)는 신경염증의 발병 요인 중의 하나로 알려져 있다. 이에 본 연구는 약용곤충으로 알려진 청동풍뎅이(Anomala albopilosa) 성충과 3령 유충(궁벵이) 에탄올 추출물을 대상으로 항산화활성을 비교 측정하였으며, 또한 대식세포인 RAW 264.7 세포에서의 NO 의 생성억제 효과 및 세포독성 그리고 분자 염증관련 인자인 iNOS, COX-2와 $PGE_2$ 생성 및 활성 억제를 조사하여 항염 증효과 등의 생리활성을 측정 비교하였다. 우선, 분자염증은 활성산소 관련의 물질과 밀접한 관련이 있으므로 향산화 실험과 관련하여 청동풍탱이 성충과 3령 유충 에탄올 추출물 의 DPPH(1,1-diphenyl-2-picrylhydrazyl) radical 소거능, superoxide radical 소거능, xanthine oxidase 저해활성 그리고 nitric oxide 소거능에 대한 항산화활성 등의 assay를 실시하였다. 그 결과, 청동풍뎅이 에탄올 추출물은 항산화능을 갖고 있었으며, 3령 유충 추출물에서보다는 성충 추출물에서 다소 높은 항산화활성을 보여주었다. 또한 RAW 264.7 세포에 LPS로 자극을 주고 청동풍뎅이 성충과 3령 유충 에탄올 추출물을 처리하여 항염증관련 활성을 확인해본 결과, 고농도에서는 다소 세포독성을 나타냈지만 농도의존적으로 NO, iNOS, COX-2 그리고 $PGE_2$의 생성 억제효과가 나타났다. 이러한 결과는 유용곤충자원을 이용한 유효성분 추출을 통한 항산화 및 항염증 물질의 연구 또는 예방하거나 치료할수 있는 염증 억제 성분의 분리 및 그 작용기전 연구에 중요한 기초 자료가 될 것이라 사료된다.

• The Korean Journal of Physiology
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• 제4권1호
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• pp.59-67
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• 1970

In recent observations on vestibular eye movements in mammals, reported by several different workers, it was indicated that the pattern of reflex eye movement from semicircular canal nerve stimulation in rabbits was different from that observed in the other species such as cats and dogs. Observing the different anatomical features of the extraocular muscles of rabbits, Kim ascribed the different pattern of eye movement of rabbits to the functional difference of inferior and superior oblique muscles from those of other species. Present experiment was carried out to elucidate a physiological mechanism underlying in such particular pattern of reflex eye movement in rabbits. An individual canal nerve was selectively stimulated, under a dissecting microscope, by a fine electrode induced into an ampulla through a hole provided on the wall of corresponding osseous canal, and responses of the extraocular muscles were checked by recording the isotonic changes of muscle length. Following results were obtained. 1. Direct stimulation of the superior or inferior oblique muscles Produced upward or downward movement of the eye turning toward medial side respectively. 2. Stimulation of the unilateral canal nerve Produced a marked contraction of a main contracting ocular muscle and simultaneous relaxation of an antagonistic muscle in both eyes. Less potent contraction of an additional ocular muscle was observed and it appeared to augment the function of the main contracting muscle in the ipsilateral eye. 3. Stimulation of superior semicircular canal nerve caused a primary contraction of superior rectus, synergic contraction of superior oblique and relaxation of inferior rectus in ipsilateral eye. Contraction of inferior oblique and relaxation of superior oblique were observed in the contralateral eye. 4. Stimulation of lateral semicircular canal nerve produced a primary contraction of medial rectus, synergic contraction of superior oblique and relaxation of lateral rectus in the ipsilateral eye. Contraction of lateral rectus and relaxation of medial rectus were observed in the contralateral eye. 5. Stimulation of inferior semicircular canal nerve produced a primary contraction of superior oblique, synergic contraction of superior rectus and relaxation of inferior oblique in the ipsilateral eye. Contraction of. inferior rectus and relaxation of superior rectus were observed in the contralateral eye. 6. Upon stimulation of individual canal nerve, the pattern of eye movement in rabbits is different from those of cats, however, the responses of the extraocular muscles appear to be similar in two species. Therefore, it is concluded that the different Pattern of eye movement in both species are not due to the possible difference of vestibule-ocular reflex pathways but to the functional difference of superior and inferior oblique muslces.

• The Korean Journal of Physiology and Pharmacology
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• 제1권3호
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• pp.275-284
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• 1997

Unilateral labyrinthectorny (ULX) causes autonomic symptoms, ocular and postural asymmetries, which disappear over tune in the process of equilibrium recovery known as vestibular compensation. In the present study in order to elucidate mechanisms responsible for the effects of electrical stimulation on vestibular compensation and investigate the relationship between vestibular compensation and c-Fos expression in the medial vestibular nuclei following ULX, we measured spontaneous nystagmus, eye movement induced by sinusoidal rotation and c-Fos expression or to 72 hs after ULX in Sprague-Dawley rats. Experimental animals were divided into two groups: ULX group with ULX only, and electrical stimulation (ES) group with electrical stimulation of $-2{\sim}-5V$, 1.0ms, 100 Hz to the lesioned vestibular system for 4 hs/day. Spontaneous nystagmus following ULX disappeared by 72 hs in ULX group and 36 hs in ES group. In eye movement induced by sinusoidal rotation, normal pattern of eye movement by rotation toward the lesioned side was recovered 24 hs after ULX at rotation of 0.1 Hz and 6 hs after at 0.2 Hz, 0.5 Hz in ULX group. In ES group, the eye movement recovered after 12 hs at 0.1 Hz, 6 hs at 0.2 Hz, and 4 hs at 0.5 Hz. Directional Preponderance which represents the symmetry of bilateral vestibular functions showed significantly early recovery in ES group compared with that of ULX group. Expression or c-Fos immunoreactive cells in the bilateral medial vestibular nuclei was severely asymmetrical till 36 hs in ULX group, and then it became a symmetry and disappeared after 72 hs. However, ES group showed the symmetry of c-Fos expression after 6 hs, which was significantly early recovery in 25 group. All these findings suggest that electrical stimulation ameliorates recovery of vestibuloocular reflex following ULX by the restoration of the balance of the resting activity between bilateral medial vestibular nuclei. In addition, c-Fos expression in the medial vestibular nuclei could be used as a marker of vestibular compensation since c-Fos expression is closely related to the course of recovery following ULX.

• 감성과학
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• 제8권4호
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• pp.423-433
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• 2005

컴퓨터 하드웨어의 발전은 예술과 기술의 결합을 더욱 결속시키는 계기가 된다. 기계간의 상호작용에서 관객이 간섭할 수 있게 되면 그 결과물은 관객이 작품을 완성한다고 한다 기술의 업그레이드가 진화하며 재사용 되는 경우를 '피드백'이라 말할 수 있다. 백남준의 '참여 TV'는 기계가 인간의 행동에 반응하여 시지각 정보를 실시간으로 행위의 정도에 따라 반응하여 결과물을 보여주는 기본 방식을 갖춘 작품이다 댄 그래함의 작품에서 시공의 피드백을 경험할 수 있다. '연속되는 과거로 이루어지는 현재'라는 설치를 통해 관객의 영상이 거울에 피드백되는 상황 속에서 모니터에서는 5초 전 관객이 했던 영상을 재생시키는 장치를 하였다. 빌 비올라의 영상과 음향의 실시간 확대라는 점에서 감성을 자극하는 공명의 피드백을 일으키고 있는 작품처럼 우연의 시간과 관객의 등장을 바탕으로 한 작가의 철학이 읽혀지는 작품으로 승화되어야 하는 것이 현대 미디어 아트가 풀어 나가야 할 근본적인 화두이다. 스텔락은 몸과 기계 그리고 신경과 두뇌가 발생시키는 신호 체계에 많은 관심을 보였다. 미디어인 몸과 터미널 디스플레이 행위자로 결국 맥루한이 말한 몸의 확장으로서의 미디어 자체가 되었다. 제프리 쇼는 '명료한 도시 legible City'에서 관객의 몸이 관여하여 페달의 속도와 핸들의 움직임에 영상이 반응한다. 작품 'RE:MARK'는 에드몽 뀨쇼(Edmond Couchot)의 '바람에 심는다.' 나 백남준의 '참여 TV'에서 보여주었듯이 마이크를 입력장치로 사용하여 인터렉션을 유도하였다. 피아간의 피드백이 없으면 커뮤니케이션도 없다. 실감을 느끼는 관객들은 작품에 감동한다 피드백 현상이 자연스러워야 하는 이유이다. 작가 정신의 숭고한 고유-독창성(Originality)이 피드백의 경로를 투과하면서 자신을 남에게 투영시켜 얻어내는 것이 고유성의 변종이다. 피드백은 단순한 작품의 일부가 아니라 작품을 이루는 뼈대이다 기술의 과시만으로는 예술의 행위가 될 수 없다. 작가의 예술성이 관객의 감성에 피드백 되도록 노력해야 한다 그러기 위해서는 예정된 피드백이란 느낌을 관객이 갖지 않도록 하여야 한다. 인터렉티브 미디어 아트는 초기의 형태에서 벗어나 새로운 집적된 피드백 기술로 전환하여야 할 시기가 온 것이다.

• The Journal of Korean Physical Therapy
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• 제12권2호
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• pp.121-132
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• 2000

This study was carried out to determine the influencing of electrical stimulation to cervicothoracic sympathetic ganglion(CTSG; stellate ganglion) u the sympathetic tone. For the purpose of this study. the stimulation was given to both the interferential current stimulation(ICS: AMF 100Hz) group consisting of 10 person(males 8, females 2) and the transcutaneous nerve electrical stimulation(TENS: 100 Hz) group of 10(males 7, females 3) in the right side of the trachea with probe electrodes. Then. the temperature changes on the surfaces of the forehead. cheek, neck and internal ear of cephalocervix, which is subject to the influence of the cervicothoracic sympathetic ganglion, and the palm. which is the end of the upper limbs. measured before. immediately alter. 10 minutes after and 20 minutes after experiment. The results are summarized as follows. 1. The emergence of remarkable Horner's symptoms which appear due to the changes of the tone of cervicothoracic sympathetic ganglion was not seen. However, in the interferential current stimulation group there were two felt the sense of warmth in the facial region and one person who felt it in the upper limbs, and in the transcutaneous nerve electrical stimulation group there was each one person who felt the sense of warmth in the facial region and in the upper limbs, respectively. Both groups have each one person who felt the sense of oppression in the eyelids. Three persons of the interferential current stimulation group and two persons of the transcutaneous nerve electrical stimulation group have the sense of hoarse voice or numbness in the neck. These are the symptoms that appeared during stimulation, so it is difficult for them to be considered as the direct effects of the changes of the tone of cervicothoracic sympathetic ganglion. 2. The t-test was performed to determine the significance between the right, which is the experimental side, and the left, which is the non-experimental side. Significance between the right, which is the experimental side, and the left, which is the non-experimental side. Significant changes were seen in the necks of the interferential current stimulation group and in the cheeks and internal ears of the transcutaneous nerve electrical stimulation immediately after experiment(P<.05). And the interferential current stimulation group showed a very high significance in the cheeks immediately after experiment and in the necks ten minutes after experiment(p<.01). Therefore, it could be seen that the electrical stimulation had an influencing on the changes of body temperature of the cephalocervix. 3. In both the interferential current stimulation group and the transcutaneous nerve electrical stimulation group, the forecheads, checks and necks of the cephalocervix in the experimental side(right) rather than the non-experimental side(left) had mostly a statistically significant rise in temperature immediately after experiment. The one-way ANOVA was carried out to determine the temperature change of on the surface of the body with the lapse of time; before, immediately after, ten minutes after and tewenty minutes after experiment. But no statistical significance was found from both the right and left sides.

• 한국콘텐츠학회논문지
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• 제12권6호
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• pp.371-379
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• 2012

본 연구는 부산 경남지역의 치과내원환자를 대상으로 주관적 구강건강인식과 상태 그리고, 치과진료 분야에 치과공포수준을 파악하여 추후 치과진료 시 치과공포를 줄일 수 있는 방안을 마련하여, 구강건강의 향상과 더불어 삶의 질 향상에 기초자료로 개발하고자 한다. DFS의 전체 문항에서 100점 만점에 남자(44.9점)보다 여자(53.1점)에서 공포감이 모두 높게 나타났다. 치료종류에 따른 공포수준은 임플란트 치료 치료회피요인(p=0.015), 교정치료 치료회피요인(p=0.002), 생리적 반응요인(p=0.009), 보철치료 치료회피요인(p=0.014), 신경치료 치료회피요인(p=0.005), 생리적 반응요인(p=0.017), 치주치료 치료회피요인(p=0.013), 생리적 반응요인(p=0.004), 정기검진을 받지 않는 경우 치료회피요인(p=0.027)이 높았다. 구강증상에 대하여 인지하고 있는 개수가 많을수록 치료회피요인(p=0.001), 자극반응요인(p=0.002), 생리적반응요인(p=0.005)로 높았다. 이상의 결과에서 치과공포를 줄이기 위해서는 정기검진과 예방적 처치를 위한 다양한 프로그램개발 및 구강보건교육을 실시해야 할 것이다.

• Restorative Dentistry and Endodontics
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• 제32권6호
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• pp.514-521
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• 2007

[ $P2X_3$ ] 수용기는 세포 외에 존재하는 ATP에 의하여 활성화되는 ligand-gated cation channel로서 염증 혹은 조직 손상 시 세포에서 유리된 ATP가 $P2X_3$ 수용기와 결합하여 동통을 유발하는 것으로 알려져 있다. 이에 본 연구는 형광면역조직화학기법을 통하여 사람의 치수에서 $P2X_3$의 발현과 바닐로이드 수용기인 TRPV1과의 공존 양상을 확인함으로써 이들의 치수동통전달 기전을 밝히고자 시행되었다. 사람의 치수에서는 치관부 및 치근부 전체에 걸쳐 다수의 $P2X_3$ 면역양성 신경섬유가 관찰되었으며 이 중 약 79.4%에서 TRPV1과의 공존을 보였다. 이상의 결과로 사람의 치수에서 $P2X_3$는 TRPV1과 더불어 치수염증 시 중추로의 동통전달과 유해자극의 증폭에 있어서 중요한 역할을 할 것으로 사료된다.