Restorative Dentistry and Endodontics

The Korean Academy of Conservative Dentistry (대한치과보존학회)
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EXPRESSION OF P2X3 AND ITS COLOCALIZATION WITH TRPV1 IN THE HUMAN DENTAL PULP

사람치수에서 P2X3의 발현 및 TRPVI과의 공존에 관한 면역조직화학적 연구

  • Kim, Young-Kyung (Department of Conservative Dentistry, School of Dentistry, Kyungpook National University) ;
  • Kim, Sung-Kyo (Department of Conservative Dentistry, School of Dentistry, Kyungpook National University)
  • 김영경 (경북대학교 치의학전문대학원 치과보존학교실) ;
  • 김성교 (경북대학교 치의학전문대학원 치과보존학교실)
  • Published : 2007.11.30
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Abstract

The purinoreceptor, $P2X_3$ is a ligand-gated cation channel activated by extracellular ATP. It has been reported that ATP can be released during inflammation and tissue damage, which in turn may activate $P2X_3$ receptors to initiate nociceptive signals. However, little is known about the contribution of $P2X_3$ to the dental pain during pulpal inflammation. Therefore, the purpose of this study was to investigate the expression of $P2X_3$ and its colocalization with TRPV1 to understand the mechanism of pain transmission through $P2X_3$ in the human dental pulp with double labeling immunofluorescence method. In the human dental pulp, intense $P2X_3$ immunoreactiyity was observed throughout the coronal and radicular pulp. Of all $P2X_3$-positive fibers examined, 79.4% coexpressed TRPV1. This result suggests that $P2X_3$ along with TRPV1 may be involved in the transmission of pain and potentiation of noxious stimuli during pulpal inflammation.

[ $P2X_3$ ] 수용기는 세포 외에 존재하는 ATP에 의하여 활성화되는 ligand-gated cation channel로서 염증 혹은 조직 손상 시 세포에서 유리된 ATP가 $P2X_3$ 수용기와 결합하여 동통을 유발하는 것으로 알려져 있다. 이에 본 연구는 형광면역조직화학기법을 통하여 사람의 치수에서 $P2X_3$의 발현과 바닐로이드 수용기인 TRPV1과의 공존 양상을 확인함으로써 이들의 치수동통전달 기전을 밝히고자 시행되었다. 사람의 치수에서는 치관부 및 치근부 전체에 걸쳐 다수의 $P2X_3$ 면역양성 신경섬유가 관찰되었으며 이 중 약 79.4%에서 TRPV1과의 공존을 보였다. 이상의 결과로 사람의 치수에서 $P2X_3$는 TRPV1과 더불어 치수염증 시 중추로의 동통전달과 유해자극의 증폭에 있어서 중요한 역할을 할 것으로 사료된다.

Keywords

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