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http://dx.doi.org/10.5395/JKACD.2007.32.6.514

EXPRESSION OF P2X3 AND ITS COLOCALIZATION WITH TRPV1 IN THE HUMAN DENTAL PULP  

Kim, Young-Kyung (Department of Conservative Dentistry, School of Dentistry, Kyungpook National University)
Kim, Sung-Kyo (Department of Conservative Dentistry, School of Dentistry, Kyungpook National University)
Publication Information
Restorative Dentistry and Endodontics / v.32, no.6, 2007 , pp. 514-521 More about this Journal
Abstract
The purinoreceptor, $P2X_3$ is a ligand-gated cation channel activated by extracellular ATP. It has been reported that ATP can be released during inflammation and tissue damage, which in turn may activate $P2X_3$ receptors to initiate nociceptive signals. However, little is known about the contribution of $P2X_3$ to the dental pain during pulpal inflammation. Therefore, the purpose of this study was to investigate the expression of $P2X_3$ and its colocalization with TRPV1 to understand the mechanism of pain transmission through $P2X_3$ in the human dental pulp with double labeling immunofluorescence method. In the human dental pulp, intense $P2X_3$ immunoreactiyity was observed throughout the coronal and radicular pulp. Of all $P2X_3$-positive fibers examined, 79.4% coexpressed TRPV1. This result suggests that $P2X_3$ along with TRPV1 may be involved in the transmission of pain and potentiation of noxious stimuli during pulpal inflammation.
Keywords
$P2X_3$ receptor; Immunofluorescence method; Human dental pulp; TRPV1; Transmission of pain;
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