• Journal of Acupuncture Research
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• v.20 no.2
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• pp.123-134
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• 2003

Objective : This experimental in vitro study investigates the effects of electro acupuncture stimuli to the DCX and PSA-NCAM positive cells in the dentate gyrus of spontaneously hypertensive rats. Methods : We used immunohistochemical methods to observe the change of neuron cells, and then counts the immunoactive cells under the $100{\times}$ visual field optical microscope. Results : 1. The counts of DCX-positive cells in the dentate gyrus significantly increased in the 2Hz group versus control group. Otherwise 100Hz group did not have a significant differance. 2. The counts of PSA-NCAM-positive cells in the dentate gyrus rather decreased in the both 2Hz group and 100Hz group versus control group. Conclusions : We find out the electro acupuncture stimuli have some effects upon cerebral neuron cell in the dentate gyrus spontaneously hypertensive rats.

• The Journal of Korean Physical Therapy
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• v.11 no.1
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• pp.71-77
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• 1999

The purpose of this study was to investigate the changes of muscle power by transcutaneous electrical nerve stimulation(TBNS), low frequency-low intensity(20pps, invisible muscle contraction intensity), low frequency-high intensity(20pps, visible muscle contraction), high frequency-low intensity(100pps, invisible muscle contraction intensity) and high frequency-high intensity(100pps, visible muscle contraction). The results were as follows. 1. Increased muscle power after 30 minutes of treatment by low frequency-low intensity TENS, and post-treatment 30 minutes muscle power were increased more than pre-treatment power(p<0.05). 2. Decreased muscle power after a 30 minute treatment by low frequency-high intensity TENS, and after the 30 minute treatment was terminated muscle power didn't recover to pre-treatment levels. 3. Decreased muscle power after 30 minute treatment by high frequency-low intensity TENS, but post-treatment 30 minute, muscle power didn't recover to pre-treatment levels. 4. The muscle power was remarkably decreased by high frequency-high intensity TENS after 30 minute treatment, in addition treatment terminated after 30minutes didn,t recover to pre-treatment power(p<0.05). 5. Lower frequency-low intensity TENS are good methods for preventing muscle fatigue, buty high intensity (TENS) are increased muscle fatigue. 6. Traditional TENS by high frequency-low intensity is a good method for preventing muscle fatigue.

• Korean Journal of Acupuncture
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• v.23 no.4
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• pp.157-167
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• 2006

목적 : 인삼은 전통적으로 학습과 기억능력 증진 및 각종 질병치료에 우수한 효과가 있는 것으로 알려져 있다. 본 연구에서는 이러한 인삼약침이 소음스트레스가 유발된 태아쥐의 해마 및 시상하부에서 nitric oxide synthase에 미치는 영향을 관찰하였다. 방법 : 인삼약침이 소음스트레스에 미치는 영향을 연구하기 위하여 태아기에 소음스트레스를 유발하고 생후 4주 후 7일간 인삼약침을 투여한 후 NADPH-d 조직화학법을 시행하였다. 결과 : 소음스트레스는 태아쥐의 해마 및 시상하부에서 NOS 발현이 유의하게 증가하였다. 2. 인삼약침은 해마 CA1에서만 소음스트레스에 의해서 증가된 NOS 발현을 유의하게 억제하였다 3. 인삼약침은 시상 하부 PVN, DMH 및 LHA에서 소음스트레스에 의해서 증가된 NOS 발현을 유의하게 억제하였다. 결론 : 본 연구를 통하여 인삼약침이 소음스트레스가 유발된 태아쥐의 해마 및 시상하부에서 증가된 NOS 발현을 유의하게 억제시켜 태아의 스트레스 자극에 인삼약침치료가 유의한 효과가 있음을 확인하였다.

• Journal of Haehwa Medicine
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• v.25 no.1
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• pp.53-62
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• 2016

Objectives : The purpose of this study was to report a case of a patient efficacy of electromagnetic acupuncture using Whata 153 in facial nerve palsy in Herpes zoster oticus. Methods : We treated the patient with magnetic acupuncture combined with electro-acupuncture. Acupuncture points were at the face (BL2, GB14, TE23, ST4, ST6, ST3). All the acupuncture points were stimulated with magnetic field and 4 of them were combined with electro-acupuncture. Results & Conclusions : The improvement of facial movement and symptom was evaluated by Yanagihara grading system(Y-system), House-Brackmann scale(HB scale) and Sunnybrook facial grading system(SFGS). After treatment, all of the scales(Y-system, HB scale and SFGS) and symptom of the patient were improved. From the above results, we suggest that magnetic acupuncture and electro-acupuncture might be effective on facial nerve palsy in Herpes zoster oticus.

• Applied Microscopy
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• v.25 no.3
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• pp.1-19
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• 1995

The present study was performed to clarify the effect of vagus nerve stimulation on the enterochromaffin(EC) cells in the body of the stomach, the first part of the duodenum and the ceceum of rats by using routine electron microscopy and immunogold labelling. The changes in the ultrastructure and in the labelling density of the gold particles of the EC cells were investigated after vagus nerve stimulation. The vagus nerve was electrically stimulated with a square wave pulse generator for a duration of 5 minutes each, a total of 8 times at 2 minute intervals. Immunogold labelling demonstrated that the epithelial serotonin immunoreactive cells of the gastrointestinal tract are EC cells containing characteristic pleomorphic granules. Immunocytochemically labelled gold particles were largely concentrated in the dense matrix of the granules of the EC cell, and the labelling density of the gold particles considerably increased after the vagus nerve stimulation. Except for a slight activation of Golgi complexes, no remarkable changes in the ultrastructures of the EC cells were noted after the vagus nerve stimulation. The above results suggest that vagus nerve stimulation may activate serotonin biosynthesis in EC cells.

• The Journal of Korean Physical Therapy
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• v.16 no.2
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• pp.63-71
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• 2004

Denervated skeletal muscle produces muscle atrophy as well as changes at the neuromuscular junction which leads to terminal axonal sprouting and an ultrastructural remodeling. NT-3 is expressed in adult muscle and motoneurons. Normally NT-3 has a potential role in regulating adult neuromuscular jungtion and recovering following muscle atrophy. Also, it could influence synaptic neurotransmission between motoneurons and skeletal muscle cells. The purpose of this study was to investigate the effect of electrical stimulation therapy(EST) on NT-3 expression in neuromuscular junction following sciatic nerve transsection in rats. After EST application during 7 days, the immunoreactivity of NT-3 was increased in neuromuscular junction

• Journal of the Korean Academy of Clinical Electrophysiology
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• v.2 no.3
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• pp.37-49
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• 2004

The purpose of this study was to determine the effect of spinal motor neuron excitability by cranial electrostimulation(CES). The fifteen Sparague-Dawley adult male rats were assigned to the three groups; GroupI(control), GroupII(low rate CES), GroupIII(high rate CES). Spinal motor neuron excitability was measured to use a computerized H reflex. The results of this study was as follows; M latency, M amplitude and H latency were no significant difference in all groups on repeated measured ANOVA(p>.05) but low rate CES and high rate CES groups were lower than ether group in comparative measurement of H amplitude and Hmax/Mmax ratio(p<.05). These results lead to the conclusion that spinal neuron excitability was influenced by CES. These results suggest that CES had the capability to lower spinal motor neuron excitability used synaptic blockade in spinal segment.

• Journal of the Korean Academy of Clinical Electrophysiology
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• v.2 no.3
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• pp.25-35
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• 2004

The purpose of this study was compared to MTICs(maximal tolerated isometric contraction), decrement and recovery. For this, using isokinetic exercise analysis device(Biodex Medical Systems Inc., Biodex System 3PRO, USA), low rate(20 Hz) and high rate(100 Hz) NMES(neuromuscular electrical stimulation) were applied to the quadriceps muscles of fifteen patients with spastic hemiplegia caused by lesions in the central nervous system. The results were as follows: 1. It was shown to fast decrement in the middle of phase at low rate NMES and to slow decrement of MTIC response at high rate NMES(p<.01). 2. It was shown to fast recovery at high rate NMES and to slow recovery at low rate NMES in recovery tendency of MTIC(p<.01). These conclusions suggest that NMES of high rate caused to slow fatigue and fast recovery different from low rate NMES.

• Annals of Clinical Neurophysiology
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• v.4 no.1
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• pp.28-33
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• 2002

Backgrounds and objective : EEG reflect dynamic changes of continuous neuronal activities by internal and external stimuli. The aim of this study is to quantify nonlinearly the local dynamic differences among EEG data corresponding to different states of brain. Methods : EEG was recorded from twelve healthy normal subjects(mean age, 29.7 years; 8 men and 4 women) using digital EEG machine. 18-channel EEG data were selected during eyes closed(EC), eyes open(EO), and mental arithmetic(MA) in each subject. Correlation dimension(D2) and largest Lyapunov exponent(LLE) were calculated from three states and average value was mapped 2 dimensionally and compared with each other. Results : The distribution of D2 was relatively symmetric and its value was higher in frontal than in parieto-occipital region during EC. These findings were reversed during EO. Bilateral centro-temporo-parietal region showed high D2 value in MA compared with those in EC, which was more prominent in left side. LLE was larger than zero in all state and showed significant differences among EC, EO and MA(p=0.000). Conclusion : These results suggest that nonlinear analysis of EEG can quantify dynamic state of brain.

• Annals of Clinical Neurophysiology
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• v.7 no.1
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• pp.13-16
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• 2005

Background: The purpose of this study is to investigate the effect of the intensive functional electrical stimulation(FES) on the improvement of the gait pattern of the chronic hemiplegic patients. Method: Six hemiplegic patients, who could walk independently but have equinovarus deformity during the gait cycle, participated in this study. The affected peroneus longus and tibialis anterior muscles of all subjects were stimulated for 2 weeks period (20 minutes duration, 6 times/day). We measured the activities (mean voltage) of those muscles during the walking, using dynamic EMG. Results: After treatment, there were significant improvements in the strength of peroneus longus and tibialis anterior muscles and the gait speed, but there was no interval change of the spasticity of plantar flexor. The mean voltages of two muscles are significantly increased in all the patients (p<0.05). Conclusion: The results showed that the intensive FES on affected peroneus longus and tibialis anterior muscles in chronic hemiplegic patients could be useful for the improvement of functional gait.