• Title/Summary/Keyword: 식품 불안정

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Antimicrobial Activity of Autoclaved Cabbage Juice (가압살균한 양배추즙액의 미생물번식 저해작용)

  • Han, Duck-Chul;Kyung, Kyu-Hang
    • Korean Journal of Food Science and Technology
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    • v.27 no.1
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    • pp.74-79
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    • 1995
  • Autoclaved juices of common vegetables including cabbage were growth inhibitory to various microorganisms. Sensitivity of microorganisms to antimicrobial action of autoclaved vegetable juices was different depending on microbial strains. Lactic acid bacteria and Gram negative bacteria were less sensitive while non-lactic Gram positive bacteria and yeasts were very much sensitive to antimicrobial action of autoclaved cabbage juice(ACJ). Staphylococcus aureus and Candida utilis whose growth were completely inhibited in ACJ could grow in ACJ diluted with distilled water. This suggests that microorganisms were not able to grow in ACJ because of growth inhibitory compounds produced during heating but not because of the lack of nutrients. Cabbage juice heated at $100^{\circ}C$ for up to 30 min was not inhibitory while that heated at $121^{\circ}C$ for 5 min was. Heating temperature was an important parameter in generating growth inhibitory compound in heated cabbage juice.

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A Study on the Extraction of Thermostable Pectinesterase from Valencia Orange (Valencia 오렌지로부터 내열성 Pectinesterase의 추출)

  • Hou, Won-Nyoung;Walker, Brigdet L.
    • Korean Journal of Food Science and Technology
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    • v.27 no.5
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    • pp.658-665
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    • 1995
  • Low yield of a thermostable pectinesterase(TSPE) from citrus fruits has made its detailed study extremely tedious and difficult; therefore, maximizing TSPE extraction is desirable. It is assumed that TSPE is bound to the cell components via ionic linkage and covalent bonds. Therefore, in this study, variations in extraction time, pH, NaCl concentration and commercial enzyme preparations were used to increase the yield of TSPE from Valencia orange. The largest recovery of TSPE, obtained by heating extracted pectinesterase(PE) at $70^{\circ}C\;for\;5{\sim}10$ minute, was achieved using actate buffer(pH 4.14) with 1 M NaCl and 0.2% $Cytolase^{TM}$ 104(a mixture of cellulase, hemicellulase and pectinase; Genecor, Inc). The two aquous phase partitioning with 5.0% Triton X-114 could be used as a tool for separation of thermolabile pectinesterase(TLPE) and TSPE from crude PE. Also, water extraction and $0{\sim}0.3$ ammonium sulfate fractionation could be used for removing non-pectinesterase protein.

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Rheological Properties of Biopolymer Produced by Alkali-Tolerant Bacillus sp. (알카리 내성 Bacillus sp.가 생산하는 생물 고분자의 리올로지적 성질)

  • Lee, Shin-Young;Kim, Jin-Young;Shim, Chang-Sub
    • Korean Journal of Food Science and Technology
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    • v.28 no.3
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    • pp.538-544
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    • 1996
  • A highly viscous biopolymer from alkali-tolerant Bacillus sp. was purified and its rheological properties were studied. 1% (w/v) solution of purified biopolymer showed pseudoplastic fluid behavior with the yield stress similar to those of xanthan and guar gum, and its consistency index was exponentially dependent on concentration and temperature. The concentration dependency of consistency index exhibited two rectilinear plots with different slopes at 1% concentration and pseudoplastic property increased with the increase of biopolymer concentration. The biopolymer solution exhibited a low temperature dependency and the activation energy of flow was 1.16 kacl/g mol. The apparent viscosity was very dependent on the change of pH and the addition of salt. However, no organic solvent effects were observed effects of viscosity synergism with the addition of viscosifier were not observed.

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Effect of Processing Conditions Upon Heat Stability and Structure Formation in Fish Protein (생선단백질의 열안정성과 조직형성속도에 공정조건이 미치는 영향)

  • Park, Sung-Jin;Kim, Byung-Yong
    • Korean Journal of Food Science and Technology
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    • v.24 no.5
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    • pp.463-469
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    • 1992
  • Effect of thermal processing upon the structure formation of surimi was investigated by differential scanning calorimeter (DSC), rigidity changes during heating, and scanning electron microscopy (SEM). DSC studies showed the transition temperatures and heat capacity of the proteins during heating. Thermal transition peaks of the proteins were shifted to the lower temperatures or disappeared by the addition of salt and higher heating rate or setting treatment ($4^{\circ}C$ or $40^{\circ}C$). Whereas setting at $4^{\circ}C$ for 24hr produced elastic component and showed a permanent effect in rigidity development during heating, setting at $40^{\circ}C$ for 30 min produced a temporary effect. SEM studies in conjunction with rigidity scanning provided an insight into the structural features of the gel and helped clarify the setting and protein modification effects.

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Effect of γ-Irradiation on the Antioxidant Activity of Rice Hull, Rice Bran and Barley Bran (감마선 조사에 의한 왕겨, 미강, 맥강의 항산화능의 변화)

  • 배성문;김정한;조철우;정태준;육홍선;변명우;이승철
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.2
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    • pp.246-250
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    • 2002
  • Effect of ${\gamma}$-irradiation to cereal processed by-products was examined for antioxidative ability. Rice hull (RH), rice bran (RB) and barley bran (BB) were irradiated with 5, 10, 15 and 20 kGy of ${\gamma}$-ray at 4.2 kGy/h. The amount of total phenol compounds of unirradiated RH, RB, and BB were 0.873 mM, 0.643 mM, and 0.377 mM, respectively. Irradiation up to 20 kGy did not show noticeable effect to the amount of total phenol compounds in RH, RB and BB. Electron donating abilities of RH, RB and BB were very similar, and they were not affected by irradiation. According to TBARS analyses, the inhibition abilities of lipid peroxidation of RH and RB were not affected by ${\gamma}$-irradiation, while those of BB were decreased with irradiation. These results indicate that BB is more sensitive to ${\gamma}$-irradiation than other rice processed by-products.

Changes of Antigen Binding Activities of Hen′s Egg Yolk Immunoglobulins after Proteolysis (효소 분해에 의한 난황 항체의 항원 결합력 변화)

  • 이경애
    • Korean journal of food and cookery science
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    • v.13 no.2
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    • pp.168-172
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    • 1997
  • Yolk immunoglobulins (yIgG) from hen's egg were purified. To investigate the stability of yIgG to digestive enzymes, the changes of antigen binding activities (ABC) after in vitro proteolysis were examined by competitive ELISA. After 30 min exposure to pepsin, the ABC of yIgG was lost. However, comparing with native yIgG, the ABC of pepsin digested yIgG was decreased, but considerable amount of ABC was remained after 30 min exposure to pepsin in 50% saccharose solution. Therefore, the stability of yIgG to pepsin digestion was improved by the addition of saccharose to yIgG solution. The ABC of yIgG was considerably remained after exposure to trypsin and chymotrypsin for 8 hr. YIgG showed especially good stability to chymotrypsin proteolysis.

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Rheological Properties of Dough with Added Corni fructus Flour (산수유 첨가에 따른 밀가루 반죽의 물리적 특성)

  • Shin, Ji-Woong;Shin, Gil-Man
    • Food Science and Preservation
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    • v.15 no.3
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    • pp.390-395
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    • 2008
  • The rheological properties of dough made with 0% 1 % 2% or 3% Corni fructus flour were investigated Rapid Visco Analyzer (RVA) analysis showed that the initial pasting temperature increased with increasing Corni fructusflour content, while the peak viscosity decreased. The water absorption, stability, development time, elasticity and valorimeter value of the dough, as determined using a farinograph and alveograph, decreased with increasing amounts of Corni fructusflour, while weakness increased. The extensibility, fermented volume and consistency of the dough increased gradually with increasing Corni fructus flour content. These results indicate that the addition of Corni fructus flour affects the rheological properties of bread.

Optimization of Microwave-Assisted Extraction under Atmospheric Pressure Condition for Soluble Ginseng Components (상압조건의 마이크로웨이브 추출공정에서 가용성 인삼성분의 추출조건 최적화)

  • Kwon, Joong-Ho;Kim, Kyung-Eun;Lee, Gee-Dong
    • Korean Journal of Food Science and Technology
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    • v.32 no.1
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    • pp.117-124
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    • 2000
  • Considering the thermal unstableness of ginseng components, microwave-assisted extraction(MAE) was performed under the atmospheric pressure condition. The monitoring of extraction characteristics and the optimization of extraction conditions were made by response surface methodology. The extraction efficiency of soluble ginseng components was high at lower ethanol concentration and at higher microwave power, while crude saponin content was easily extracted at higher ethanol concentration. Estimated conditions for the maximized extraction of soluble components including crude saponin, total phenolics and electron donating ability were $54{\sim}60%$ in ethanol concentration, $41{\sim}90$ W in microwave power, and within 4 min in extraction time. Predicted values at the optimum condition(60% ethanol, 80 W microwave power and 4 min extraction time) were in good agreement with observed values.

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Effect of Copigmentation on the Stability of Anthocyanins from a Korean Pigmented Rice Variety (Copigmentation에 의한 유색미 안토시아닌의 안정화 효과)

  • Yoon, Joo-Mi;Hahn, Tae-Ryong;Yoon, Hye-Hyun
    • Korean Journal of Food Science and Technology
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    • v.30 no.4
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    • pp.733-738
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    • 1998
  • Tannic acid (TA), chlorogenic acid (GA) and caffeic acid (CA) as copigments were added in anthocyanin solutions (pH 3.0) to test their effects on the stability against temperatures, metal ions, and light. Absorbance of anthocyanins increased with the increase of copigment concentrations. Tannic acid showed the highest absorbance increase with bathochromic shift of 14 nm (from 514 nm to 528 nm). Copigmentation resulted in higher storage stability of anthocyanins for 21 days at room temperature $925^{\circ}C)$ but lower thermal stability at higher temperatures more than $70^{\circ}C. Addition of metal ions to TA caused the enhanced stability as well as increased absorbance of anthocyanins. Copigmentation also caused a significant increase of light stablilty of anthocyanins at room temperature, so that addition of TA showed 2.3 times showed photodegradation of anthocyanins under 20,000 lux-light dosage in pH 3.0 solution at $25^{\circ}C$.

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Stability of Pigment Produced by Monascus pilosus (Monascus pilosus가 생성하는 색소의 안정성)

  • Park, Mee-Ja;Yoon, Eun-Kyung;Kim, Soon-Dong
    • Korean Journal of Food Science and Technology
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    • v.34 no.4
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    • pp.541-545
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    • 2002
  • Stability of Monascus pilosus pigment was investigated under various conditions. The concentration of the pigments stored under air and sub-atmosphere (250 mmHg) for 30 days at room temperature decreased by 77.9% and 48.4%, respectively. The pigment solution was stable under temperature ranges of $20-80^{\circ}C$, pH 4-8, darkness and presence of KCl, NaCl, $CaCl_2$, $MgCl_2$, and $ZnCl_2$. In contrast, the pigment solution was relatively unstable, decreasing in concentration by 6.0 and 11.6% at 100 and $121^{\circ}C$, 15.5 and 13.7% at pH 3 and 9, 22.9 and 66.8% under fluorescence and sun light, respectively, and 20.2% in the presence of $AlCl_3$.