• Title/Summary/Keyword: 식별방법

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Plant regeneration and transformation of grape (Vitis labrusca L.) via direct regeneration method (포도 (Vitis labrusca L.)의 직접 재분화 방법을 이용한 식물체 재분화와 형질전환)

  • Kim, Se Hee;Shin, Il Sheob;Cho, Kang Hee;Kim, Dae Hyun;Kim, Hyun Ran;Kim, Jeong Hee;Lim, Sun-Hyung;Kim, Ki Ok;Lee, Hyang Bun;Do, Kyung Ran;Hwang, Hae Seong
    • Journal of Plant Biotechnology
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    • v.40 no.4
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    • pp.210-216
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    • 2013
  • Efficient regeneration methods and transformation system are a priority for successful application of genetic engineering to vegetative propagated plants such as grape (Vitis labrusca L.). This research is to establish shoot regeneration system from plant explants for 'Campbell Early', 'Tamnara', 'Heukgoosul', 'Heukbosek' using two types of plant growth regulators supplemented to medium. The highest adventitious shoot regeneration rate of 5% was achieved on a medium containing of Murashige and Skoog (MS) inorganic salts and Linsmaier and Skoog (LS) vitamins, 2 mg/L of TDZ and 0.1 mg/L of IBA. Leaf tissue of 'Campbell Early', was co-cultivated with Agrobacterium strains, LBA4404 containing the vector pBI121 carrying with CaMV 35S promoter, gus gene as reporter gene and resistance to kanamycin as selective agent, the other Agrobacterium strains, GV3101 containing the vector pB7 WG2D carrying with mPAP1-D gene. mPAP1-D is a regulatory genes of the anthocyanin biosynthetic pathway. 'Campbell Early' harboring mPAP1-D gene was readily able to be selected by red color due to anthocyanin accumulation in the transformed shoot. These results might be helpful for further studies to enhance the transformation efficiency in grape.

Development of the Accident Prediction Model for Enlisted Men through an Integrated Approach to Datamining and Textmining (데이터 마이닝과 텍스트 마이닝의 통합적 접근을 통한 병사 사고예측 모델 개발)

  • Yoon, Seungjin;Kim, Suhwan;Shin, Kyungshik
    • Journal of Intelligence and Information Systems
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    • v.21 no.3
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    • pp.1-17
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    • 2015
  • In this paper, we report what we have observed with regards to a prediction model for the military based on enlisted men's internal(cumulative records) and external data(SNS data). This work is significant in the military's efforts to supervise them. In spite of their effort, many commanders have failed to prevent accidents by their subordinates. One of the important duties of officers' work is to take care of their subordinates in prevention unexpected accidents. However, it is hard to prevent accidents so we must attempt to determine a proper method. Our motivation for presenting this paper is to mate it possible to predict accidents using enlisted men's internal and external data. The biggest issue facing the military is the occurrence of accidents by enlisted men related to maladjustment and the relaxation of military discipline. The core method of preventing accidents by soldiers is to identify problems and manage them quickly. Commanders predict accidents by interviewing their soldiers and observing their surroundings. It requires considerable time and effort and results in a significant difference depending on the capabilities of the commanders. In this paper, we seek to predict accidents with objective data which can easily be obtained. Recently, records of enlisted men as well as SNS communication between commanders and soldiers, make it possible to predict and prevent accidents. This paper concerns the application of data mining to identify their interests, predict accidents and make use of internal and external data (SNS). We propose both a topic analysis and decision tree method. The study is conducted in two steps. First, topic analysis is conducted through the SNS of enlisted men. Second, the decision tree method is used to analyze the internal data with the results of the first analysis. The dependent variable for these analysis is the presence of any accidents. In order to analyze their SNS, we require tools such as text mining and topic analysis. We used SAS Enterprise Miner 12.1, which provides a text miner module. Our approach for finding their interests is composed of three main phases; collecting, topic analysis, and converting topic analysis results into points for using independent variables. In the first phase, we collect enlisted men's SNS data by commender's ID. After gathering unstructured SNS data, the topic analysis phase extracts issues from them. For simplicity, 5 topics(vacation, friends, stress, training, and sports) are extracted from 20,000 articles. In the third phase, using these 5 topics, we quantify them as personal points. After quantifying their topic, we include these results in independent variables which are composed of 15 internal data sets. Then, we make two decision trees. The first tree is composed of their internal data only. The second tree is composed of their external data(SNS) as well as their internal data. After that, we compare the results of misclassification from SAS E-miner. The first model's misclassification is 12.1%. On the other hand, second model's misclassification is 7.8%. This method predicts accidents with an accuracy of approximately 92%. The gap of the two models is 4.3%. Finally, we test if the difference between them is meaningful or not, using the McNemar test. The result of test is considered relevant.(p-value : 0.0003) This study has two limitations. First, the results of the experiments cannot be generalized, mainly because the experiment is limited to a small number of enlisted men's data. Additionally, various independent variables used in the decision tree model are used as categorical variables instead of continuous variables. So it suffers a loss of information. In spite of extensive efforts to provide prediction models for the military, commanders' predictions are accurate only when they have sufficient data about their subordinates. Our proposed methodology can provide support to decision-making in the military. This study is expected to contribute to the prevention of accidents in the military based on scientific analysis of enlisted men and proper management of them.

A Study on the Forest Vegetation Classification and Analysis of Interspecific Association in Mt. Munsu and Mt. Okseok (문수산.옥석산 일대의 산림식생유형 분류와 종간연관 분석)

  • Kim, Hye-Jin;Yun, Chung-Weon
    • Journal of Korean Society of Forest Science
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    • v.98 no.4
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    • pp.379-391
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    • 2009
  • This study was carried out to classify forest vegetation structure of Mt. Munsu and Mt. Okseok from May to September in 2008 using phytosociological analysis methodology of Z-M school under the purpose of furnishing basic materials for rational and scientific forest management. Total of one hundred five study sites (quadrats) was investigated in the area. Quercus mongolica community group was divided into 5 communities such as Pinus densiflora for. erecta community, Hydrangea serrata for. acuminata community, Salix gracilistyla community, Pinus koraiensis community and Larix kaempferi community. Pinus densiflora for. erecta community was subdivided into Rhododendron micranthum group, Quercus variabilis group and Pinus densiflora for. erecta typical group. Hydrangea serrata for. acuminata community also into Ligularia fischeri group, Deutzia glabrata group (into Juglans mandshurica subgroup, Prunus maackii subgroup and Callicarpa japonica subgroup). Accordingly, the vegetation of the study areas could be described as 1 community group, 5 communities, 5 groups, and 3 subgroups and as 10 units in total. Classified vegetation units were highly correlated with altitude and topography. According the analysis of interspecific association, it was shown as three types which were coincident with differential species and character species on the constancy table.

Development of Loop-mediated Isothermal Amplification (LAMP) for Detection of Vibrio alginolyticus (Loop-mediated isothermal amplification (LAMP)법을 이용한 Vibrio alginolyticus의 신속 진단법 개발)

  • Hong, Seung-hyun;Heo, Moon-Soo
    • Journal of Life Science
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    • v.25 no.8
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    • pp.903-909
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    • 2015
  • Loop-mediated isothermal amplification (LAMP), a PCR-based diagnostic method, is based on autocycling strand displacement DNA synthesis in the presence of exonuclease-negative Bst DNA polymerase under isothermal conditions. With the help of four specific primers that recognize six different sequences of a target DNA, LAMP has high specificity in pathogenic identification in a short time. Hence, in the present study, LAMP is used as a diagnostic tool in the identification of the most dreadful aquatic pathogenic species, Vibrio alginolyticus, and to develop species-specific LAMP primers and optimization of LAMP reaction conditions such as annealing temperature, elongation time, and other PCR chemical concentrations, including MgSO4, dNTPs, Betaine, and Bst polymerase. The optimized LAMP primers were also checked for specificity with other Vibrio species, which showed that the designed primers were very specific to V. alginolyticus After the first introduction of a species name like this one, the first part (“Vibrio” in this case) should be abbreviated to only the first letter.only. These are usually the most harmful pathogens of the Vibrio species that appear in shrimp and crabs. The results also revealed that the LAMP assay could be 10-fold more sensitive than conventional PCR in detecting V. alginolyticus. This could be the first report on using a rapid and highly sensitive technique, the LAMP assay, in the effective diagnosis of the pathogenic bacteria V. alginolyticus, which could help in the early detection of diseases, particularly in aquaculture.

IFC Property Set-based Approach for Generating Semantic Information of Steel Box Girder Bridge Components (IFC Property Set을 활용한 강박스교 구성요소의 의미정보 생성)

  • Lee, Sang-Ho;Park, Sang Il;Park, Kun-Young
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.34 no.2
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    • pp.687-697
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    • 2014
  • This study ranges from planning phase to the detailed design phase of steel box girder bridge and proposes ways to generate semantic information of components through Industry Foundation Classes (IFC), a data model for Building Information Modeling (BIM). The classification of components of steel box girder bridge was performed to define information items required for identifying semantic information based on IFC, and spatial information items based on topology and physical information items based on functions of components were classified to create additional properties that does not support IFC by applying user-defined property set within the IFC framework. Steel box girder bridge information model based on IFC was implemented through BIM software and semantic information input interface, which was developed in this study to examine the effectiveness of the additionally created user-defined property. Furthermore, the quantity take-off of components was performed through information model of steel box girder bridge, and the applicability of the proposed method was tested by comparing the quantity take-off based on design document with the result.

Utilization of Ground Control Points using LiDAR Intensity and DSM (LiDAR 반사강도와 DSM을 이용한 지상기준점 활용방안)

  • Lim, Sae-Bom;Kim, Jong-Mun;Shin, Sang-Cheol;Kwon, Chan-O
    • Spatial Information Research
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    • v.18 no.5
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    • pp.37-45
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    • 2010
  • AT(Aerial Triangulation) is the essential procedure for creating orthophoto and transforming coordinates on the photographs into the real world coordinates utilizing GCPs (Ground Control Point) which is obtained by field survey and the external orientation factors from GPS/INS as a reference coordinates. In this procedure, all of the GCPs can be collected from field survey using GPS and Total Station, or obtained from digital maps. Collecting GCPs by field survey is accurate than GCPs from digital maps; however, lots of manpower should be put into the collecting procedure, and time and cost as well. On the other hand, in the case of obtaining GCPs from digital maps, it is very difficult to secure the required accuracy because almost things at each stage in the collecting procedure should rely on the subjective judgement of the performer. In this study, the results from three methods have been compared for the accuracy assessment in order to know if the results of each case is within the allowance error: for the perceivable objects such as road boarder, speed bumps, constructions etc., 1) GCPs selection utilizing the unique LiDAR intensity value reflected from such objects, 2) using LiDAR DSM and 3) GCPs from field survey. And also, AT and error analysis have been carried out w ith GCPs obtained by each case.

A Development of Analysis System for Vessel Traffic Display and Statistics based on Maritime-BigData (해상-빅데이터 기반 선박 항적 표시 및 해상교통량 통계 분석 시스템의 개발)

  • Hwang, Hun-Gyu;Kim, Bae-Sung;Shin, Il-Sik;Song, Sang-Kee;Nam, Gyeung-Tae
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.20 no.6
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    • pp.1195-1202
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    • 2016
  • Recently, a lot of studies that applying the big data technology to various fields, are progressing actively. In the maritime domain, the big data is the meaningful information which makes and gathers by the navigation and communication equipment from the many ships on the ocean. Also, importance of the maritime safety is emphasized, because maritime accidents are rising with increasing of maritime traffic. To support prevention of maritime accidents, in this paper, we developed a vessel traffic display and statistic system based on AIS messages from the many vessels of maritime. Also, to verify the developed system, we conducted tests for vessel track display function and vessel traffic statistic function based on two test scenarios. Therefore, we verified the effectiveness of the developed system for vessel tracks display, abnormal navigation patterns, checking failure of AIS equipments and maritime traffic statistic analyses.

Relationship between Characteristics of Accounting Firms and Audit Engagement Risks based on Bayesian Network (베이지안 네트워크를 기반으로 한 회계법인의 속성과 감사계약체결위험간의 관계)

  • Sun, Eun-Jung;Park, Sung-Jin
    • Management & Information Systems Review
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    • v.36 no.1
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    • pp.1-19
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    • 2017
  • One of the methods of securing the reliability of accounting information is maintaining high audit quality. The first step of improving audit quality is lowering audit engagement risks. Thus, this study analyzed the relationship between the characteristics of accounting firms and audit engagement risks based on the Bayesian Network. For this, Markov Blanket, the minimum explanatory variable set, which affects audit engagement risks, was presented, and based on the drawn causal relationship, sensitivity analysis was conducted to verify the characteristics of accounting firms, which affect audit engagement risks. The existing preceding research that used multiple regression analysis presumes the linearity between explanatory variables and dependent variables, so there was a limit in drawing the relationship between explanatory variables. Therefore, this study figured out the interdependence between variables using the General Bayesian Network and examined the impact that each variable has finally on audit engagement risks that affects the audit quality. The results of this study would greatly contribute to improving the efficiency of the supervisory task by allowing a supervisory institution to identify an accounting firms that does not manage audit engagement risks properly and to improve the supervision of the accounting firms in advance. In addition, this study will be used as a reference when a supervisory institution would improve the system related to audit quality by presenting the characteristics of accounting firms related to the audit quality.

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Study on Developmental Strategy of National Defense & IT Convergence (국방IT융합기술의 발전전략에 관한 연구)

  • Koo, Han Lim;Kim, Seong Min;Rhee, Woo Seop
    • Convergence Security Journal
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    • v.14 no.6_1
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    • pp.99-112
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    • 2014
  • National Defense & IT Convergence is ensuring actual force integration by developing new IT Convergence Technology for the purpose of realizing the concept of Network Centric Warfare. The Ministry of Defense has appointed National Defense & IT Convergence Center of Defense Agency for Technology and Quality as a specialized agency for the National Defense & IT Convergence development, a mission in the creative economy era and has been active in military demand inquiry and prior technology plan with the industrial-academic research institute for discovery of National Defense IT Convergence projects based on the Convergence Center. Further, it is promoting focused on the timely introduction of private IT new technology by operating the project planning team comprised of defense domain specialists and by applying the advanced planning method etc. Accordingly, for the development of National Defense & IT convergence, developmental strategy is urgently required to be utilized effectively after considering theoretically on the national IT Convergence policy enforcement and phenomenon of National Defense & IT Convergence technology and analyzing them comprehensively. This thesis promotes in phases the IT Convergence projects which are identified for the development, improves the efficiency of newly created National Defense & IT Convergence center and suggests training program of IT Convergence personnel related to the defense reforms. With such, it will achieve smart defense in advance and will also contribute largely for the realization of the immediate paradigm shift and of the creative economy.

A Study on Maintenance Cost Model for Establishing a Strategies of Port Facility Maintenance (항만시설 유지관리 전략수립을 위한 비용모델연구)

  • Park, Miyun;Lee, Jeonghun;Park, Sangwoo;Lim, Jonggwon
    • Journal of the Society of Disaster Information
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    • v.16 no.2
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    • pp.276-290
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    • 2020
  • Purpose: The construction history of domestic port facilities has been more than 100 years, and until recently, modern facilities have been continuously built and expanded. However, it is not easy to keep the required performance conditions at the time of initial construction due to changes in the marine environment and increase in volume. In particular, in the case of harbor structures that have a long service life, safety performance and function management are becoming very important due to the increase in the size of ships, the increasing frequency of use, and the increase in the scale of natural disasters. Method: Therefore, this study investigates the state change by structural type of port facilities and analyzes the rehabilitation activities and the history that contribute to the performance improvement and life extension activities. Result: Through this, we distinguished between performance improvement cost (CAPEX) and repair maintenance activity (OPEX) that can be used to establish port facility maintenance strategy, and suggested cost model that can be used to establish maintenance strategy. Conclusion: These studies are expected to contribute greatly to mid- to long-term investment decisions.