• The Korean Journal of Ecology
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• v.23 no.1
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• pp.65-70
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• 2000

Field collections were made from six gypsy moth (Lymantria dispar L.) Populations in Kyonggy and Cheju areas during the period 1987∼1997. Characteristics of gypsy moth populations at different phases were examined in terms of egg mass density, relative larval density, plant damage, and fecundity. Males captured in pheromone trap were recorded, and we examined if there was a relationship between numbers captured and the population density during the following generation. Egg mass density was closely related with larval density, and furthermore these densities were correlated with the level of plant damage, indicating that larval dispersion was limited in oviposition areas. The gypsy moth population cycle was short in Korea with the period from population development to innocuous level usually lasting 2∼3 years. Male caught by pheromone trap (mean number of males caught per trap per day in peak emergence period) was positively correlated with egg mass densities in the following season (r²=0.93). A low fecundity was detected from outbreak populations which accompanied defoliation. Fecundity of gypsy moth ranged from 538 to 601 at other phases.

• Korean Journal of Ecology and Environment
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• v.38 no.2 s.112
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• pp.217-224
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• 2005

This study was aimed to analyze the long-term fluctuation of water quality and phytoplankton dynamics of Paldang reservoir in Korea and to assess the relationship between algal bloom patterns and hydrological, limnological data. Diatoms in Paldang reservoir occurred continuously through the year. Blue- green algae occurred during the summer season (from June to Sept.), and the highest count was observed in July. Occurrence pattern of green algae was similar to that of blue-green algae. The rest of algae contained a lot of Cryptomonas spp. whose concentration was high from May to Aug. Dominant algal genera (>>7,000 cells $mL^{-1}$) in Paldang reservoir were Aulacoseira, Cyclotella, Microcystis, and Cryptomonas spp. Microcystis and Anabaena occurred during the summer season. Many different green algal genera were found in Paldang reservoir but their abundances were very low. There were some significant correlations (r>0.3, p<0.05) between algal taxa and water quality; diatoms and water temperature, TP:blue-green algae and water temperature, pH, DO saturation, COD, TP; green algae and water temperature, pH, DO saturation, COD, SS, TP. Furthermore, algal genera and water quality was significantly correlated (r>0.3, p<0.05) ; Aulacoseira and TN, TP; Anabaena and water temperature, DO saturation, COD, TP : Microcystisand water temperature, pH, DO saturation, TP; Coelastrum and COD, SS; Scenedesmus and water temperature, COD, TN, TP; Cryptomonas and DO saturation, TN. In Paldang reservoir, the water temperature had relatively big effect on blue-green algal bloom that was also dependant upon its hydrologic condition.

• Korean Journal of Plant Resources
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• v.27 no.4
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• pp.271-278
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• 2014

Rubus coreanus has been used as a traditional medicine in Asia because of its various pharmacological properties. This study examined the effects of protocatechuic acid (PCA), one of phenolic compounds derived from R. coreanus on the lipid metabolism in high cholesterol diet-induced mice. A total of 30 male C57BL/6 mice were divided into 5 groups with 6 mice in each group as follows: (1) Control mice received normal diet (ND). (2) Mice received high-cholesterol diet (HCD) plus water, 10% sucrose solution and treated daily oral phosphate-buffered-saline (PBS) of equal volumes through gavage. (3) Mice received HCD and treated daily with 25 mg/kg b.w./day of PCA (4) with 50 mg/kg b.w./day or (5) with 10 mg/kg b.w./day of simvastatin via oral gavage for 12 weeks. Body weights were measured weekly for a period of experiment. After treatment, liver, thymus, spleen and kidney were harvested and weighed, and the lipid metabolite profiles (total cholesterol, triglyceride (TG), HDL-cholesterol (HDL-c), LDL-cholesterol (LDL-c) and liver-damaging markers (GOT and GPT) in serum were examined. PCA significantly reduced the total cholesterol, TG, LDL-c level and increased the HDL-c level. PCA administration also significantly reduced the levels of GOT and GPT. These results indicate that the PCA could be used as a functional material for lowering lipid and an adjuvant for the treatment of hyperlipemia.

• Journal of Life Science
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• v.25 no.7
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• pp.833-838
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• 2015

Multidrug-resistant super bacterial, fungal, viral, and parasitic infections are major health threaten pathogens. However, to overcome the present healthcare situation, among the leading alternatives to current drugs are antimicrobial peptides (AMPs), which are abundantly produced via various species in nature. AMPs, small host defense proteins, are in charge of the innate immunity for the protection of multicellular organisms such as fish, amphibian, reptile, plants and animals from infection. The number of AMPs identified per year has increased steadily since the 1980s. Over 2,000 natural AMPs from bacteria, protozoa, fungi, plants, and animals have been listed into the antimicrobial peptide database (APD). The majority of these AMPs (>86%) possess 11–50 amino acids with a net charge from 0 to +7 and hydrophobic percentages between 31–70%. This report classified AMP into several categories including biological source, biological functions, peptide properties, covalent bonding pattern, and 3D structure. AMP functions not only antimicrobial activity but facilitates cell biological activity such as chemotatic activity. In addition, fibroblastic reticular cell (FRC) originated from mouse lymph node stroma induced the expression of AMP in inflammatory condition. AMP induced from FRC contained whey acidic protein (WAP) domain. It suggests that the classification of AMP will be done by protein domain.

• Applied Biological Chemistry
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• v.45 no.3
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• pp.173-178
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• 2002

Graft cultivation of Paeonia and Moutan was attempted to improve the harvest yields of medicinal parts of the plants. When Paeonia root was grafted with Moutan stem, the harvest yields of underground roots showed 35% increase, with Paeonia radix and Moutan cortex ratio of 3 : 1 paeoniflorin and paeonol contents among grafted Paeonia radix (11.71 and 0.35 mg/g, respectively), Paeonia radix (9.91 and 0.80 mg/g, respectively), and Moutan cortex (10.81 and 2.84 mg/g, respectively) were not significantly different. Paeonia radix grafted with Moutan stem was cultivated to investigate the ecological properties and to compare index components, including minerals, fatty acids, and organic acids. The contents of 10 major minerals among the samples were very similar, whereas those of Fe were different, showing 86.06, 127.36, 75.05, and 140.63 mg/g for (Paeonia radix, Paeonia radix grafted, Moutan cortex, and Moutan cortex grafted) contents and GC profiles of 11 fatty acids including linoleic, palmitic, linolenic, and oleic acids and those of 6 organic acids including oxalic, citric, and malic acids were very similar. Results of sensory evaluations were very similar between Paeonia radix and Paeonia radix grafted or Moutan cortex and Moutan cortex grafted showed astringent taste, and stronger sensory intensity of Paeonia radix than Paeonia radix grafted showed bitter taste, and stronger sensory intensity of Moutan cortex than Moutan cortex grafted.

• Korean Journal of Environment and Ecology
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• v.32 no.3
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• pp.274-288
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• 2018

This study investigated the environmental factors and fish assemblage in 42 wetlands between spring and autumn of 2017 to evaluate the fish distribution and deduce the management strategy for improving biodiversity in created wetlands located at the Nakdong River basin. The investigation identified a total of 30 fish species and found that the most of wetlands were dominated by exotic fishes such as Micropterus salmoides and Lepomis macrochirus. Fish species such as Rhinogobius brunneus, Opsariichthys uncirostris amurensis, Zacco platypus were less abundant in the area with high density of Micropterus salmoides (static area) because they preferred the environment with active water flow. The pattern analysis of fish distribution in each wetland using the self-organizing map (SOM) showed a total of 24 variables (14 fish species and 10 environmental variables). The comparison of variables indicated that the distribution of fish species varied according to water depth and plant cover rate and was less affected by water temperature, pH, and dissolved oxygen. The plant cover rate was strongly associated with high fish density and species diversity. However, wetlands with low plant biomass had diversity and density of fish species. The results showed that the microhabitat structure, created by macrophytes, was an important factor in determining the diversity and abundance of fish communities because the different species compositions of macrophytes supported diverse fish species in these habitats. Based on the results of this study, we conclude that macrophytes are the key components of lentic freshwater ecosystem heterogeneity, and the inclusion of diverse plant species in wetland construction or restoration schemes will result in ecologically healthy food webs.

• Journal of Life Science
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• v.17 no.12
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• pp.1649-1653
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• 2007

High temperature stresses have caused growth inhibition and delayed heading in highland cultivation Chinese cabbage during summer in Korea. We have studied high temperature stress responses in the terms of changes of inorganic components and proteins by proteomic analyses. Insufficiencies of nitrogen and phosphorus have affected growth rate and calcium deficiency has caused blunted heading. Proteins extracted from Brassica seedling grown at the altitude of 600m and 900m in the Mount Jilun were extracted and analysed by 2-dimentional polyacrylamide gel electrophoresis. Profiles of protein expression was then analyzed by 2-dimentional gel analyses. Protein spots showing different expression level were picked using the spot handling workstation and subjected to MALDI-TOF MS. Total 48 protein spots were analyzed by MALDI-TOF MS and 30 proteins spots out of 48 were identified by peptide mass fingerprinting analyses. Fourteen proteins were up-regulated in extracts from the altitude of 900m and they were identified as oxygen-evolving proteins, rubisco activase and ATPase etc. Sixteen proteins were up-regulated in extracts from the altitude of 600m and they were identified as glutathione S-transferase(1, 28kD cold induced- and 24 kD auxin-binding proteins) and salt-stress induced protein etc. These stress-induced proteins were related to the mediated protective mechanism against oxidative damage during various stresses. The results indicated that physiological phenomenon in response to high temperature stresses might be resulted by complex and multiple array of responses with drought, heat, oxidative, salt, and cold by high temperature.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.53-53
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• 2019

방울다다기양배추는 아직까지 국내에서는 소비자들에게 인지도가 낮은 작물이지만 최근 식생활 및 소비 패턴이 변화하면서 편의성, 차별성 등이 중요시면서 미니 과일, 미니채소류에 대한 관심이 증가하고 있다. 또한 외국의 도입 채소류에 대한 관심이 증가하면서 2013년 방울다다기양배추 수입량이 36톤이었던 것이 2015년에는 14.3배가 증가한 515톤으로 증가하였다. 이를 통해 방울다다기양배추가 가지고 있는 기능성과 미니채소에 대한 관심으로 지속적으로 소비가 증가할것으로 예상되고 있다. 이와같이 증가추세에 있는 방울다다기양배추의 수입을 대체하기 위해서는 국내 재배면적 확대가 필요하나 저온성 작물인 방울다다기양배추는 고온기 남부지역에서는 재배가 어려워 제주지역의 겨울재배 작형으로 제한되어 재배가 되고 있다. 따라서 제주지역의 겨울재배 작형과 연계하여 강원도 고랭지의 서늘한 여름 기후를 이용하여 단경기인 9~11월에 생산할 수 있는 여름재배 작형을 개발할 필요성이 있어 시험을 수행하였다. 본 시험은 2018년~2019년 태백 철암의 노지(표고 750 m)에서 시험품종은 "브릴리언트"를 이용하여 3월 하순 파종하여 50일 육묘하여 5월 10일 포장에 정식하였다. 흑색멀칭한 시험포장에 $80{\times}30cm$부터 $80{\times}60cm$까지 10 cm 간격으로 차이를 두고 정식하였으며 정식 전 N-P-K = 20-15-20 kg/10 a를 시비하였고 시험포장은 수확까지 병해충 방제 등 주기적으로 관리하면서 생육특성 및 수량성을 조사하였다. 재식거리별 생육상황을 비교한 결과 초장은 처리간 큰 차이가 없었고 경경은 재식거리가 커질수록 증가하는 경향이었다. 또한 엽장 및 엽폭은 처리간의 경향이 없었으며 엽수는 재식거리가 커질수록 증가하였고 $80{\times}30cm$ 처리시 80매내외였던 것이 $80{\times}50cm$ 이상일 경우 100매 이상으로 증가하였다. 재식거리별 구의 특성을 조사한 결과 구경은 처리간 큰 차이는 없었으나 재식거리가 커질수록 구경이 다소 증가하였으며 구중도 증가하는 경향이었다. 방울다다기양배추의 구의 엽수는 처리간 큰 차이가 없었고 경도 및 당도도 큰 차이가 없었다. 처리별 수량은 $80{\times}40cm$와 $80{\times}50cm$ 처리에서 수량이 다소 증가하였고 상품화율은 $80{\times}50{\sim}60cm$ 처리에서 80%이상으로 높았다. 따라서 방울다다기양배추의 여름 고랭지 재배시 재식거리는 $80{\times}50cm$ 처리시 생육 및 엽수 증가에 따른 구의 형성이 증가하여 수량성이 높아지고 상품화율도 증가하였다.

• Food Engineering Progress
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• v.15 no.4
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• pp.370-375
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• 2011

In this study the polymerase chain reaction (PCR) combined with denaturing gradient gel electrophoresis (DGGE) was evaluated as a method permitting the rapid detection of pathogens in fresh originally grown vegetables. A universal primer (341GCf/534r) was selected for its ability to amplify the V3 region of 16S-rRNA genes in their target pathogens (Salmonella typhimurium, Pseudomonas fluorescens, Bacillus cereus, Listeria monoytogenes, Staphyloocus aureus, E. coli). The 194 bp fragments in PCR were successfully duplicated as expected. The amplified fragments of the same size from six different pathogens also showed good separation upon DGGE. The detection limit of PCR-DGGE for six pathogens in fresh-cut lettuces were over $10^{5}$ CFU/g when sampled by stomaching. However, when the sampling method was changed from stomaching to shaking, the detection limit of six pathogens in organic vegetables was shown to increase by over $10^{1}$ CFU/g, but only those of B. cereus were over $10^{3}$ CFU/g. Therefore, PCR-DGGE was shown to be a reliable method for the detection of pathogens in fresh-cut vegetables.

• The Journal of the Convergence on Culture Technology
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• v.5 no.3
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• pp.229-239
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• 2019

Rapid economic development has changed the dietary habits and patterns. Especially, western style diet has increased the risk of type 2 diabetes (T2DM) in Korea. To provide more specific and appropriate diet guideline for the prevention and for the treatment of T2DM, the investigation on the characteristics of diebetic patients related to the diet needs to be done. In this study, we therefore analyzed eating behaviors, dietary attitudes, and intake of food and nutrients of normal subjects (control, n=26) and diabetic patients (case, n=18) diagnosed T2DM within one year in Daegu Gyeongbuk area. Body mass index of the patients were significantly higher than the control (p<0.05). Overeating, high fried food and low whole grain intake were revealed as risk factors for T2DM. From the food frequency questionnaire analysis, salty foods such as fermented soybean paste (Doenjang) and watery Kimch intake were associated with T2DM. Intake of vegetable lipid, ${\beta}$-carotene, calcium, copper, and vitamin K were also shown to be associated with T2DM. Taken together, these findings suggest that maintaining ideal body weight and intake the foods with low salt, fat, and refined grain in appropriate amount may help to prevent and to manage T2DM.